APOL3 (NM_030644) Human Tagged ORF Clone

CAT#: RC218012

APOL3 (Myc-DDK-tagged)-Human apolipoprotein L, 3 (APOL3), transcript variant alpha/b

Product Images

Circular map for RC218012

• 

  Western blot validation of overexpression lysate (Cat# LY410766) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218012 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: APOL3
• Synonyms: apoL-III; APOLIII; CG12_1; CG121
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218012 representing NM_030644
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCAGAAAAGAAACGCTTTACTGAAGAGGCCACCAAATACTTCCGGGAGAGAGTCAGCCCAGTGC
  ATCTGCAAATCCTGCTGACTAACAATGAAGCCTGGAAGAGATTCGTGACTGCGGCTGAATTGCCCAGGGA
  TGAGGCAGATGCTCTCTACGAAGCTCTGAAGAAGCTTAGAACATATGCAGCTATTGAGGACGAATATGTG
  CAGCAGAAAGATGAGCAGTTTAGGGAATGGTTTTTGAAAGAGTTTCCCCAAGTCAAGAGGAAGATCCAGG
  AGTCCATAGAAAAGCTTCGTGCCCTTGCAAATGGTATTGAAGAGGTCCACAGAGGCTGCACCATCTCCAA
  TGTGGTGTCCAGCTCCACTGGCGCTGCCTCTGGCATCATGTCCCTTGCTGGTCTTGTTTTGGCACCATTT
  ACAGCAGGGACGAGTCTGGCCCTTACTGCAGCTGGGGTAGGGCTGGGAGCAGCGTCTGCTGTGACTGGGA
  TCACCACCAGCATCGTGGAGCACTCATACACATCATCAGCAGAAGCTGAAGCCAGCAGGCTGACTGCAAC
  CAGCATTGACCGATTGAAGGTATTTAAGGAAGTTATGCGTGACATCACACCCAACTTACTTTCCCTTCTT
  AATAATTATTACGAAGCCACACAAACCATTGGGAGTGAAATCCGTGCCATCAGGCAAGCCAGAGCCAGGG
  CCCGACTCCCTGTGACCACCTGGCGAATCTCAGCTGGAAGTGGTGGTCAAGCAGAGAGAACGATTGCAGG
  CACCACCCGGGCAGTGAGCAGAGGAGCCCGGATCCTGAGTGCGACCACTTCAGGCATCTTCCTTGCACTG
  GATGTGGTCAACCTTGTATACGAGTCAAAGCACTTGCATGAGGGGGCAAAGTCTGCATCTGCTGAGGAGC
  TGAGGCGGCAGGCTCAGGAGCTGGAGGAGAATCTAATGGAGCTCACTCAGATCTATCAGCGTCTGAATCC
  ATGCCATACCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218012 representing NM_030644
  Red=Cloning site Green=Tags(s)
  
  MDSEKKRFTEEATKYFRERVSPVHLQILLTNNEAWKRFVTAAELPRDEADALYEALKKLRTYAAIEDEYV
  QQKDEQFREWFLKEFPQVKRKIQESIEKLRALANGIEEVHRGCTISNVVSSSTGAASGIMSLAGLVLAPF
  TAGTSLALTAAGVGLGAASAVTGITTSIVEHSYTSSAEAEASRLTATSIDRLKVFKEVMRDITPNLLSLL
  NNYYEATQTIGSEIRAIRQARARARLPVTTWRISAGSGGQAERTIAGTTRAVSRGARILSATTSGIFLAL
  DVVNLVYESKHLHEGAKSASAEELRRQAQELEENLMELTQIYQRLNPCHTH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030644
• ORF Size: 993 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030644.2
• RefSeq Size: 2328 bp
• RefSeq ORF: 996 bp
• Locus ID: 80833
• UniProt ID: O95236
• Cytogenetics: 22q12.3
• MW: 36.4 kDa
• Gene Summary: This gene is a member of the apolipoprotein L gene family, and it is present in a cluster with other family members on chromosome 22. The encoded protein is found in the cytoplasm, where it may affect the movement of lipids, including cholesterol, and/or allow the binding of lipids to organelles. In addition, expression of this gene is up-regulated by tumor necrosis factor-alpha in endothelial cells lining the normal and atherosclerotic iliac artery and aorta. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]