ARHGEF11 (NM_014784) Human Tagged ORF Clone

CAT#: RC217976

ARHGEF11 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 11 (ARHGEF11), transcript variant 1

Product Images

Circular map for RC217976

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARHGEF11
• Synonyms: GTRAP48; PDZ-RHOGEF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217976 representing NM_014784
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTGTAAGGTTACCCCAGAGTATAGACAGGTTAAGTAGCCTGTCTTCTCTGGGAGATTCTGCACCAG
  AGCGCAAGTCCCCTTCCCACCATCGCCAGCCTTCGGATGCCTCTGAGACAACAGGTCTCGTTCAACGCTG
  TGTCATTATCCAAAAGGACCAGCATGGCTTCGGCTTCACAGTCAGTGGGGATCGCATTGTTCTGGTGCAG
  TCTGTGCGGCCTGGAGGTGCAGCCATGAAGGCCGGTGTGAAAGAGGGCGACCGGATCATCAAAGTCAACG
  GCACCATGGTGACCAATAGCTCACACCTGGAAGTGGTAAAGCTGATCAAATCTGGCGCCTATGTCGCACT
  CACCCTCCTGGGCTCTTCACCTTCATCCATGGGCATCTCTGGGCTCCAGCAGGACCCATCCCCAGCAGGA
  GCTCCCCGAATCACGTCAGTGATCCCCTCACCACCACCTCCTCCACCTCTACCACCTCCACAACGCATCA
  CAGGACCCAAACCTCTGCAGGATCCCGAAGTTCAAAAACATGCCACCCAGATCCTCAGGAATATGCTGAG
  GCAGGAAGAAAAAGAATTACAGGACATACTTCCACTATATGGTGACACCAGCCAGAGACCATCAGAAGGC
  CGGCTCTCTCTGGATTCCCAGGAGGGGGACAGTGGCTTGGACTCTGGGACAGAACGCTTTCCTTCCCTCA
  GTGAGTCATTGATGAATCGGAACTCGGTACTGTCAGACCCTGGGCTAGACAGTCCTCGAACCTCCCCTGT
  GATCATGGCCAGGGTGGCCCAGCACCACAGGCGGCAGGGCTCGGATGCAGCAGTCCCCTCAACCGGTGAC
  CAGGGTGTAGATCAAAGCCCAAAGCCTTTAATTATTGGCCCAGAGGAAGACTATGACCCGGGTTATTTCA
  ACAACGAGAGCGACATCATATTCCAGGATCTGGAGAAACTGAAGTCTCGGCCAGCTCACCTGGGGGTTTT
  TCTACGTTACATCTTCTCTCAGGCGGACCCCAGTCCACTGCTTTTTTACCTGTGTGCAGAAGTTTATCAG
  CAGGCAAGCCCCAAGGATTCCCGAAGCTTGGGGAAAGACATCTGGAATATTTTCCTGGAGAAAAATGCGC
  CTCTGAGAGTGAAGATCCCTGAGATGCTACAGGCTGAAATTGACTCGCGCCTGCGGAACAGCGAAGATGC
  CCGTGGTGTTCTCTGTGAAGCTCAAGAGGCAGCCATGCCTGAGATCCAAGAGCAGATCCACGACTACAGA
  ACGAAGCGCACACTGGGGCTGGGCAGCCTGTATGGTGAAAATGACCTGCTGGACCTGGATGGGGACCCTC
  TCCGAGAGCGCCAAGTGGCTGAGAAGCAGCTGGCTGCCCTTGGAGATATTTTGTCCAAGTATGAGGAAGA
  CAGGAGCGCCCCCATGGACTTCGCCCTCAATACCTACATGAGCCATGCTGGGATCCGTCTTCGAGAGGCA
  CGACCTTCCAACACAGCTGAAAAGGCCCAGTCTGCTCCTGACAAGGACAAGTGGCTACCGTTCTTCCCTA
  AGACCAAGAAGAGCAGCAATTCCAAGAAAGAAAAGGATGCCTTGGAGGACAAGAAGCGAAACCCTATCCT
  CAAATACATTGGGAAGCCCAAAAGCTCTTCTCAAAGCACATTTCATATTCCCTTGTCCCCTGTGGAAGTC
  AAACCAGGCAATGTGAGGAACATCATTCAGCACTTTGAGAACAACCAGCAGTATGATGCCCCAGAACCTG
  GGACACAACGACTCTCGACCGGAAGCTTTCCTGAGGACCTGCTGGAGAGTGACAGTTCACGCTCAGAGAT
  TCGCCTGGGCCGCTCTGAAAGCCTCAAGGGCCGGGAAGAGATGAAACGGTCTCGAAAGGCAGAGAACGTG
  CCCCGCTCTCGCAGTGATGTTGACATGGATGCTGCTGCGGAGGCTACTCGCCTGCACCAGTCAGCCTCGT
  CCTCTACCTCCAGCCTCTCCACCAGGTCTCTTGAGAACCCAACCCCTCCATTCACTCCCAAAATGGGCCG
  CAGGAGCATTGAGTCCCCCAGTTTGGGGTTCTGCACAGATACCCTCCTTCCCCACCTCCTAGAGGATGAT
  CTGGGCCAGCTGTCTGACCTGGAGCCAGAGCCAGATGCCCAAAATTGGCAGCATACAGTGGGCAAGGATG
  TGGTGGCTGGGCTAACCCAGCGGGAGATTGACCGGCAAGAGGTCATCAATGAGCTGTTTGTGACTGAAGC
  TTCCCACCTGCGCACACTCCGGGTCCTGGACCTGATCTTCTACCAGCGAATGAAGAAGGAGAACCTGATG
  CCCCGGGAGGAGCTGGCCCGGCTCTTCCCGAACCTGCCTGAACTCATAGAGATTCACAATTCCTGGTGTG
  AAGCCATGAAGAAGCTCCGGGAGGAAGGCCCCATCATCAAAGAGATCAGTGACCTCATGCTGGCCCGGTT
  TGATGGCCCTGCCCGAGAGGAACTCCAGCAAGTGGCTGCACAGTTCTGTTCCTATCAGTCAATAGCCCTA
  GAGCTAATCAAGACCAAGCAACGCAAGGAGAGTCGATTCCAGCTCTTCATGCAGGAGGCTGAGAGCCACC
  CTCAGTGTCGGCGGCTGCAGCTGAGAGACCTCATCATCTCTGAGATGCAGCGGCTCACCAAGTACCCGCT
  GCTGCTGGAGAGCATCATCAAGCACACAGAGGGTGGCACCTCTGAGCATGAGAAGCTGTGCCGGGCCCGG
  GACCAGTGCCGGGAGATTCTCAAGTATGTGAATGAAGCGGTAAAACAAACAGAGAACCGCCACCGTTTAG
  AGGGCTACCAGAAACGCCTGGATGCCACCGCCCTGGAGAGGGCCAGCAACCCCCTGGCAGCAGAGTTCAA
  GAGCCTGGATCTTACAACCAGAAAAATGATCCATGAGGGACCCCTGACCTGGAGGATCAGCAAGGATAAG
  ACCTTGGACCTCCACGTGCTGCTGCTGGAGGACCTCCTAGTGCTGCTACAGAAACAGGATGAGAAGCTAT
  TGCTGAAGTGCCACAGCAAGACTGCTGTGGGCTCCTCAGACAGCAAGCAGACCTTCAGCCCCGTGCTCAA
  GCTCAATGCTGTGCTCATCCGCTCTGTGGCCACAGATAAACGGGCCTTCTTCATCATCTGCACCTCCAAG
  CTGGGCCCACCCCAGATCTATGAGCTGGTTGCATTGACGTCATCAGACAAGAACACATGGATGGAGCTCT
  TAGAAGAGGCCGTGCGGAATGCCACCAGGCACCCCGGAGCTGCCCCAATGCCCGTCCATCCTCCACCCCC
  AGGTCCCCGGGAGCCAGCCCAGCAGGGCCCCACACCCAGCAGGGTAGAACTGGATGACTCAGACGTGTTC
  CATGGTGAACCTGAACCTGAGGAGCTGCCTGGAGGCACTGGGTCCCAGCAGAGGGTCCAAGGGAAGCACC
  AGGTCCTGCTAGAGGACCCTGAGCAGGAGGGCAGTGCAGAGGAAGAGGAACTGGGTGTCCTGCCTTGCCC
  TTCCACATCCCTGGATGGAGAGAACAGGGGCATCAGGACAAGGAACCCCATCCACTTGGCCTTCCCAGGC
  CCTCTGTTCATGGAAGGGCTCGCTGACTCCGCTCTGGAAGATGTGGAGAACCTGCGACATCTGATCCTGT
  GGAGCCTGCTGCCAGGTCACACCATGGAAACTCAGGCTGCCCAGGAGCCCGAGGACGACCTGACACCCAC
  ACCTTCTGTCATCAGCGTCACCTCTCACCCCTGGGACCCAGGCTCCCCAGGGCAAGCACCCCCTGGGGGT
  GAAGGGGACAACACCCAGCTTGCAGGGCTGGAGGGGGAACGGCCAGAGCAGGAAGACATGGGTCTCTGTT
  CTCTGGAACACCTACCCCCAAGGACCAGAAATTCTGGGATATGGGAGTCTCCAGAACTGGACAGGAATCT
  GGCTGAAGATGCTTCAAGCACAGAGGCAGCAGGAGGTTACAAAGTTGTGAGAAAAGCTGAGGTGGCAGGC
  AGCAAGGTTGTCCCTGCACTACCAGAGAGTGGCCAGTCAGAGCCTGGGCCACCTGAAGTGGAAGGCGGAA
  CAAAGGCTACGGGGAACTGCTTTTATGTCAGCATGCCATCAGGACCCCCGGACTCAAGCACCGACCACTC
  AGAGGCACCCATGAGCCCCCCTCAGCCTGACAGCCTCCCTGCAGGGCAGACAGAGCCTCAGCCTCAGCTG
  CAGGGAGGCAACGATGATCCAAGACGCCCCAGCCGCTCTCCTCCAAGCCTGGCCCTCAGGGACGTGGGCA
  TGATCTTCCATACCATTGAGCAGCTCACTCTCAAGCTCAACAGGCTCAAGGATATGGAGCTGGCCCACAG
  AGAGCTGCTCAAGTCCCTTGGGGGAGAGTCATCTGGTGGCACCACGCCTGTGGGCAGTTTCCACACAGAA
  GCAGCTAGATGGACAGATGGCTCCCTCTCACCTCCCGCTAAGGAGCCCCTAGCTTCTGACTCCAGGAACA
  GCCATGAACTGGGGCCCTGCCCTGAGGATGGCTCTGACGCCCCCCTGGAAGACAGCACAGCAGACGCAGC
  CGCGTCACCAGGACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217976 representing NM_014784
  Red=Cloning site Green=Tags(s)
  
  MSVRLPQSIDRLSSLSSLGDSAPERKSPSHHRQPSDASETTGLVQRCVIIQKDQHGFGFTVSGDRIVLVQ
  SVRPGGAAMKAGVKEGDRIIKVNGTMVTNSSHLEVVKLIKSGAYVALTLLGSSPSSMGISGLQQDPSPAG
  APRITSVIPSPPPPPPLPPPQRITGPKPLQDPEVQKHATQILRNMLRQEEKELQDILPLYGDTSQRPSEG
  RLSLDSQEGDSGLDSGTERFPSLSESLMNRNSVLSDPGLDSPRTSPVIMARVAQHHRRQGSDAAVPSTGD
  QGVDQSPKPLIIGPEEDYDPGYFNNESDIIFQDLEKLKSRPAHLGVFLRYIFSQADPSPLLFYLCAEVYQ
  QASPKDSRSLGKDIWNIFLEKNAPLRVKIPEMLQAEIDSRLRNSEDARGVLCEAQEAAMPEIQEQIHDYR
  TKRTLGLGSLYGENDLLDLDGDPLRERQVAEKQLAALGDILSKYEEDRSAPMDFALNTYMSHAGIRLREA
  RPSNTAEKAQSAPDKDKWLPFFPKTKKSSNSKKEKDALEDKKRNPILKYIGKPKSSSQSTFHIPLSPVEV
  KPGNVRNIIQHFENNQQYDAPEPGTQRLSTGSFPEDLLESDSSRSEIRLGRSESLKGREEMKRSRKAENV
  PRSRSDVDMDAAAEATRLHQSASSSTSSLSTRSLENPTPPFTPKMGRRSIESPSLGFCTDTLLPHLLEDD
  LGQLSDLEPEPDAQNWQHTVGKDVVAGLTQREIDRQEVINELFVTEASHLRTLRVLDLIFYQRMKKENLM
  PREELARLFPNLPELIEIHNSWCEAMKKLREEGPIIKEISDLMLARFDGPAREELQQVAAQFCSYQSIAL
  ELIKTKQRKESRFQLFMQEAESHPQCRRLQLRDLIISEMQRLTKYPLLLESIIKHTEGGTSEHEKLCRAR
  DQCREILKYVNEAVKQTENRHRLEGYQKRLDATALERASNPLAAEFKSLDLTTRKMIHEGPLTWRISKDK
  TLDLHVLLLEDLLVLLQKQDEKLLLKCHSKTAVGSSDSKQTFSPVLKLNAVLIRSVATDKRAFFIICTSK
  LGPPQIYELVALTSSDKNTWMELLEEAVRNATRHPGAAPMPVHPPPPGPREPAQQGPTPSRVELDDSDVF
  HGEPEPEELPGGTGSQQRVQGKHQVLLEDPEQEGSAEEEELGVLPCPSTSLDGENRGIRTRNPIHLAFPG
  PLFMEGLADSALEDVENLRHLILWSLLPGHTMETQAAQEPEDDLTPTPSVISVTSHPWDPGSPGQAPPGG
  EGDNTQLAGLEGERPEQEDMGLCSLEHLPPRTRNSGIWESPELDRNLAEDASSTEAAGGYKVVRKAEVAG
  SKVVPALPESGQSEPGPPEVEGGTKATGNCFYVSMPSGPPDSSTDHSEAPMSPPQPDSLPAGQTEPQPQL
  QGGNDDPRRPSRSPPSLALRDVGMIFHTIEQLTLKLNRLKDMELAHRELLKSLGGESSGGTTPVGSFHTE
  AARWTDGSLSPPAKEPLASDSRNSHELGPCPEDGSDAPLEDSTADAAASPGP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014784
• ORF Size: 4566 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014784.4
• RefSeq Size: 6784 bp
• RefSeq ORF: 4569 bp
• Locus ID: 9826
• UniProt ID: O15085
• Cytogenetics: 1q23.1
• Domains: RhoGEF, RGS, PDZ, PH
• Protein Families: Druggable Genome
• Protein Pathways: Vascular smooth muscle contraction
• MW: 167.7 kDa
• Gene Summary: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. A similar protein in rat interacts with glutamate transporter EAAT4 and modulates its glutamate transport activity. Expression of the rat protein induces the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]