CTNNAL1 (NM_003798) Human Tagged ORF Clone

CAT#: RC217961

CTNNAL1 (Myc-DDK-tagged)-Human catenin (cadherin-associated protein), alpha-like 1 (CTNNAL1)

Product Images

Circular map for RC217961

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  Western blot validation of overexpression lysate (Cat# LY418424) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217961 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CTNNAL1 protein (Cat# TP317961). The protein was produced from HEK293T cells transfected with CTNNAL1 cDNA clone (Cat# RC217961) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CTNNAL1
• Synonyms: ACRP; alpha-CATU; CLLP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217961 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCCTCTCCCGGACCCGCCGGCGTTGGCGGCGCCGGAGCAGTCTACGGCTCCGGCTCTTCGGGCT
  TCGCCCTCGACTCGGGACTGGAGATCAAAACTCGCTCGGTGGAGCAGACGCTACTCCCGCTGGTTTCTCA
  GATCACCACGCTTATTAATCATAAAGATAATACCAAAAAGTCTGATAAAACTCTGCAAGCAATTCAGCGT
  GTAGGACAAGCTGTCAACTTGGCAGTTGGAAGATTTGTTAAAGTAGGAGAAGCTATAGCCAATGAAAACT
  GGGATTTGAAAGAAGAAATAAATATTGCTTGTATTGAAGCTAAACAAGCAGGAGAAACAATTGCAGCACT
  TACAGACATAACCAACTTGAACCATCTGGAATCTGATGGGCAGATCACAATTTTTACAGACAAAACAGGA
  GTGATAAAGGCTGCAAGATTACTTCTTTCTTCAGTGACAAAAGTGTTGTTGCTGGCAGACCGAGTAGTCA
  TTAAACAGATAATAACATCAAGAAATAAGGTTCTCGCAACTATGGAAAGACTAGAGAAAGTGAATAGCTT
  TCAAGAGTTTGTCCAAATATTCAGTCAATTTGGAAATGAAATGGTGGAGTTTGCACATCTGAGTGGAGAT
  AGACAAAATGATTTGAAAGATGAAAAGAAAAAGGCAAAAATGGCAGCAGCTAGGGCAGTTCTTGAAAAGT
  GTACAATGATGCTTCTCACAGCTTCAAAGACATGTCTGAGGCATCCTAACTGCGAATCAGCCCATAAAAA
  CAAAGAAGGAGTATTTGACCGTATGAAAGTGGCATTGGATAAGGTCATTGAAATTGTGACTGACTGTAAA
  CCGAATGGAGAGACTGACATTTCATCTATCAGTATTTTTACTGGAATTAAGGAATTCAAGATGAATATTG
  AAGCTCTTCGGGAGAATCTTTATTTTCAGTCCAAAGAGAACCTTTCTGTGACATTGGAAGTCATCTTGGA
  GCGTATGGAGGACTTTACTGATTCTGCCTACACCAGCCATGAGCACAGAGAACGCATCTTGGAACTGTCA
  ACTCAGGCGAGAATGGAACTGCAGCAGTTAATTTCTGTGTGGATTCAAGCTCAAAGCAAGAAAACAAAAA
  GCATCGCTGAAGAACTGGAACTCAGTATTTTGAAAATCAGTCACAGTCTTAATGAACTTAAGAAAGAACT
  TCATAGTACAGCGACACAGCTGGCAGCAGATCTATTAAAATACCATGCTGATCATGTGGTTCTAAAAGCA
  TTAAAACTTACTGGAGTAGAAGGAAATTTAGAAGCTTTGGCTGAATATGCCTGTAAACTCTCTGAACAGA
  AAGAGCAGCTTGTTGAGACCTGTCGATTGTTACGACACATATCTGGGACAGAACCTCTGGAAATAACCTG
  TATACATGCAGAGGAGACATTTCAGGTGACTGGCCAACAGATAATTTCTGCTGCTGAAACATTGACATTG
  CATCCATCTAGTAAAATTGCTAAAGAAAACCTAGATGTATTTTGTGAAGCTTGGGAATCCCAAATTAGTG
  ACATGTCAACACTGCTGAGAGAAATCAATGACGTGTTTGAAGGAAGACGAGGAGAGAAGTATGGCTACCT
  TTCACTTCCAAAGCCAATGAAGAATAATGCAAACCTGAAATCATTAAAGCCAGACAAGCCTGACTCTGAG
  GAGCAAGCCAAGATAGCAAAGCTTGGACTTAAGCTGGGTTTGCTCACCTCTGACGCTGACTGCGAAATTG
  AGAAGTGGGAAGATCAGGAGAATGAGATTGTTCAATATGGACGGAACATGTCCAGTATGGCCTATTCTCT
  GTATTTATTTACTAGAGGAGAGGGGCCACTGAAAACTTCCCAGGATTTAATTCATCAACTAGAGGTTTTT
  GCTGCAGAGGGTTTAAAGCTTACTTCCAGTGTTCAAGCTTTTTCAAAACAGCTGAAAGACGATGACAAGC
  TTATGCTTCTCCTGGAAATAAACAAGCTAATTCCTCTATGCCACCAGCTCCAGACAGTAACTAAGACTTC
  TTTGCAGAATAAAGTATTTCTAAAGGTTGACAAGTGTATTACGAAGACAAGATCCATGATGGCTCTCTTA
  GTCCAACTTCTTTCACTTTGTTATAAACTGCTGAAGAAGCTTCAGATGGAAAATAACGGATGGGTCTCAG
  TTACAAATAAGGACACTATGGATAGTAAAACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217961 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAASPGPAGVGGAGAVYGSGSSGFALDSGLEIKTRSVEQTLLPLVSQITTLINHKDNTKKSDKTLQAIQR
  VGQAVNLAVGRFVKVGEAIANENWDLKEEINIACIEAKQAGETIAALTDITNLNHLESDGQITIFTDKTG
  VIKAARLLLSSVTKVLLLADRVVIKQIITSRNKVLATMERLEKVNSFQEFVQIFSQFGNEMVEFAHLSGD
  RQNDLKDEKKKAKMAAARAVLEKCTMMLLTASKTCLRHPNCESAHKNKEGVFDRMKVALDKVIEIVTDCK
  PNGETDISSISIFTGIKEFKMNIEALRENLYFQSKENLSVTLEVILERMEDFTDSAYTSHEHRERILELS
  TQARMELQQLISVWIQAQSKKTKSIAEELELSILKISHSLNELKKELHSTATQLAADLLKYHADHVVLKA
  LKLTGVEGNLEALAEYACKLSEQKEQLVETCRLLRHISGTEPLEITCIHAEETFQVTGQQIISAAETLTL
  HPSSKIAKENLDVFCEAWESQISDMSTLLREINDVFEGRRGEKYGYLSLPKPMKNNANLKSLKPDKPDSE
  EQAKIAKLGLKLGLLTSDADCEIEKWEDQENEIVQYGRNMSSMAYSLYLFTRGEGPLKTSQDLIHQLEVF
  AAEGLKLTSSVQAFSKQLKDDDKLMLLLEINKLIPLCHQLQTVTKTSLQNKVFLKVDKCITKTRSMMALL
  VQLLSLCYKLLKKLQMENNGWVSVTNKDTMDSKT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003798
• ORF Size: 2202 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003798.4
• RefSeq Size: 2573 bp
• RefSeq ORF: 2205 bp
• Locus ID: 8727
• UniProt ID: Q9UBT7
• Cytogenetics: 9q31.3
• Protein Families: Druggable Genome
• MW: 81.9 kDa
• Gene Summary: May modulate the Rho pathway signaling by providing a scaffold for the Lbc Rho guanine nucleotide exchange factor (ARHGEF1).[UniProtKB/Swiss-Prot Function]