PCDHB9 (NM_019119) Human Tagged ORF Clone

CAT#: RC217914

PCDHB9 (Myc-DDK-tagged)-Human protocadherin beta 9 (PCDHB9)

Product Images

Circular map for RC217914

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCDHB9
• Synonyms: PCDH-BETA9; PCDH3H
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217914 representing NM_019119
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACCAGGGGGTTCAGCTTTCCAAGACAAAGGCAAGTCCTGTTTCTTTTTCTTTTCTGGGGAGTGT
  CCTTGGCAGGTTCTGGGTTTGGACGTTATTCGGTGACTGAGGAAACAGAGAAAGGATCCTTTGTGGTCAA
  TCTGGCAAAGGATCTGGGACTAGCAGAGGGGGAGCTGGCTGCAAGGGGAACCAGGGTGGTTTCCGATGAT
  AACAAACAATACCTGCTCCTGGATTCACATACCGGGAATTTGCTCACAAATGAGAAACTGGACCGAGAGA
  AGCTGTGTGGCCCTAAAGAGCCCTGTATGCTGTATTTCCAAATTTTAATGGATGATCCCTTTCAGATTTA
  CCGGGCTGAGCTGAGAGTCAGGGATATAAATGATCACTCGCCAGTGTTTCGGCACAAAGAGATGGTCTTA
  AAAATATCAGAAAATACAGCTGAAGGGACAGCATTTAGACTAGAAAGAGCACAGGATCCAGATGAAGGTC
  ATAACAGTATCCAAAACTACACGATCAGCTCCAACTCTTTTTTCCATATTAAAATTAGTGGCAGTGATGA
  AGGCATGATATATCCAGAGCTAGTGTTGGACAAAGCACTGGATCGGGAGGAGCAGGAAGAGCTCAGCTTA
  ACCCTCACAGCGCTGGATGGTGGGTCTCCATCCAGGTCTGGGACCTCCACTATACGCATTGTGGTCTTGG
  ATGTCAATGACAATGCCCCACAGTTTGCCCAGGCTCTGTATGAGACCCAGGCTCCAGAAAACAGTCCAGT
  AGGGTCCCTTATTGTTAAAGTGTCTGCAGGAGATGCAGACTCAGGAGTCAATGCAGAAGTATCCTATTCA
  TTTTTTGATGCTTCTGAAGATATTTTAACAACGTTTCAAATCAATCCTTTTTCTGGGGAAATCTTTCTCA
  GAGAATTGCTTGATTATGAGTTAGTAAATTCTTACAAAATAAATATACAGGCAATGGACGGCGGAGGCCT
  TTCTGCAAGATGTACAGTTTTGATAAAAGTATTAGATTCCAATGACAATCCTCCTGAACTGATCATATCA
  TCACTTTCCAACTCTGTTGCTGAAAACTCTCCTGGGATAGTATTGGCTGTTTTTAAGATTAAAGACAGAG
  ACTCCGGAGAAAATGGAAAGACAATTTGCTATGTTCAAGATAATCTGCCTTTTTTTCTGAAACCGTCTGT
  TGACAATTTTTACATCCTAATGACTGAAGGTGCACTGGACAGAGAGAGCAAAGCTGAGTACAACATCACC
  ATCACCGTCACTGACTTGGGGACACCCAGGCTGAAAACCGAGCACAGCATAACCCTGCAGGTCTCCGACG
  TCAATGACAACGCCCCCGCCTTCACCCAAACCTCCTACACCCTGTTCGTCCGGGAGAACAACAGCCCCGC
  CCTGCACATCGGCAGTGTCAGCGCCACAGACAGAGACTCAGGCACCAACGCCCAGGTCACCTACTCGCTG
  CTGCCGCCCCAGGACCCACACCTGCCCCTCGCCTCCCTGGTCTCCATCAACGCGGACAATGGCCACCTGT
  TTGCCCTCAGGTCGCTGGACTACGAGGCCCTGCAGGCTTTCGACTTCCGCGTGGGCGCCTCAGACCGCGG
  CTCCCCGGCTTTGAGCAGCGAGGCGCTGGTGCGCGTACTGGTGCTGGACGCCAACGACAACTCGCCCTTC
  GTGCTGTACCCGCTGCAGAACGGCTCCGCGCCCTGCACCGAGCTGGTGCCCCGGGCGGCCGAGCCGGGCT
  ACCTGGTGACCAAGGTGGTGGCGGTGGACGGCGACTCGGGCCAGAACGCCTGGCTGTCGTACCAGCTGCT
  CAAGGCCACGGAGCCCGGGCTGTTCGGTGTGTGGGCGCACAATGGGGAGGTGCGCACCGCCAGGCTGCTG
  AGCGAGCGCGACGCGGCCAAGCACAGGCTGGTGGTGCTTGTCAAGGACAATGGCGAGCCTCCTCGCTCGG
  CCACCGCCACGCTGCACGTGCTCCTGGTGGACGGCTTCTCCCAGCCCTACCTGCCTCTCCCGGAGGCGGC
  CCCGGCCCAGGCCCAGGCCGACTTGCTCACCGTCTACCTGGTGGTGGCGTTGGCCTCGGTGTCTTCGCTC
  TTCCTCCTCTCGGTGCTCCTGTTCGTGGCGGTGCGGCTGTGCAGGAGGAGCAGGGCGGCCTCGGTGGGTC
  GCTGCTCGGTGCCCGAGGGTCCTTTTCCAGGGCATCTGGTGGACGTGAGCGGCACCGGGACCCTGTTCCA
  GAGCTACCAGTACGAGGTGTGTCTGACTGGAGGTTCAGAGACCGGCGAGTTCAAGTTCTTGAAGCCGATT
  ACCCCCCACCTCCCGCCCCATAGGGGTGGGAAAGAAATAGAGGAAAATTCTACTCTCCCCAATAGCTTTG
  GATTTAATTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217914 representing NM_019119
  Red=Cloning site Green=Tags(s)
  
  MKTRGFSFPRQRQVLFLFLFWGVSLAGSGFGRYSVTEETEKGSFVVNLAKDLGLAEGELAARGTRVVSDD
  NKQYLLLDSHTGNLLTNEKLDREKLCGPKEPCMLYFQILMDDPFQIYRAELRVRDINDHSPVFRHKEMVL
  KISENTAEGTAFRLERAQDPDEGHNSIQNYTISSNSFFHIKISGSDEGMIYPELVLDKALDREEQEELSL
  TLTALDGGSPSRSGTSTIRIVVLDVNDNAPQFAQALYETQAPENSPVGSLIVKVSAGDADSGVNAEVSYS
  FFDASEDILTTFQINPFSGEIFLRELLDYELVNSYKINIQAMDGGGLSARCTVLIKVLDSNDNPPELIIS
  SLSNSVAENSPGIVLAVFKIKDRDSGENGKTICYVQDNLPFFLKPSVDNFYILMTEGALDRESKAEYNIT
  ITVTDLGTPRLKTEHSITLQVSDVNDNAPAFTQTSYTLFVRENNSPALHIGSVSATDRDSGTNAQVTYSL
  LPPQDPHLPLASLVSINADNGHLFALRSLDYEALQAFDFRVGASDRGSPALSSEALVRVLVLDANDNSPF
  VLYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFGVWAHNGEVRTARLL
  SERDAAKHRLVVLVKDNGEPPRSATATLHVLLVDGFSQPYLPLPEAAPAQAQADLLTVYLVVALASVSSL
  FLLSVLLFVAVRLCRRSRAASVGRCSVPEGPFPGHLVDVSGTGTLFQSYQYEVCLTGGSETGEFKFLKPI
  TPHLPPHRGGKEIEENSTLPNSFGFNY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019119
• ORF Size: 2391 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_019119.3, NP_061992.2
• RefSeq Size: 4220 bp
• RefSeq ORF: 2394 bp
• Locus ID: 56127
• UniProt ID: Q9Y5E1
• Cytogenetics: 5q31.3
• Domains: CA
• Protein Families: Transmembrane
• MW: 83.9 kDa
• Gene Summary: This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections. [provided by RefSeq, Jul 2008]