ZNF189 (NM_003452) Human Tagged ORF Clone

CAT#: RC217848

ZNF189 (Myc-DDK-tagged)-Human zinc finger protein 189 (ZNF189), transcript variant 1

Product Images

Circular map for RC217848

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF189
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217848 representing NM_003452
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCCCCGAGCCCCCCGCCGGAGTCGAAGGGGTTGCTGACATTTGAGGATGTGGCTGTGTTTTTTA
  CCCAGGAGGAGTGGGATTATCTGGACCCAGCTCAGAGAAGCCTGTATAAAGATGTCATGATGGAGAATTA
  TGGAAACCTGGTCTCACTGGATGTTTTGAACAGAGATAAGGATGAGGAGCCAACTGTAAAACAAGAGATT
  GAAGAAATTGAGGAAGAAGTGGAACCACAGGGTGTAATAGTTACAAGAATCAAAAGTGAAATTGACCAGG
  ATCCTATGGGTAGAGAAACATTTGAACTTGTTGGTAGGTTAGATAAACAAAGAGGGATCTTCCTATGGGA
  AATACCAAGGGAATCTTTGACCCAGGAACAGAGAATGTTCAGAGAAAACACTAACATTATCCGTAAAAGA
  CCAAACTCAGAAGAGAAATGCCATAAATGTGAAGAATGTGGAAAGGGTTTTGTCCGCAAGGCCCATTTCA
  TTCAACATCAAAGGGTCCATACTGGTGAGAAACCTTTTCAGTGCAATGAATGTGGGAAAAGTTTTAGTCG
  CAGTTCATTTGTTATTGAACATCAGAGAATTCACACTGGGGAAAGGCCCTATGAGTGTAATTACTGTGGA
  AAAACCTTTAGTGTGAGCTCAACCCTTATTAGACATCAGAGAATCCACACTGGAGAAAGACCCTATCAGT
  GTAATCAGTGTAAACAGAGCTTCAGCCAGAGAAGGAGCCTTGTTAAACATCAAAGGATTCATACAGGTGA
  GAAACCCCATAAATGTAGTGACTGTGGGAAAGCCTTCAGTTGGAAATCACACCTTATTGAGCATCAAAGA
  ACTCACACTGGTGAGAAACCTTATCACTGTACCAAATGTAAGAAGAGCTTTAGTCGAAATTCATTGCTTG
  TTGAGCATCAAAGAATTCACACTGGGGAAAGACCCCATAAATGTGGTGAATGTGGGAAAGCCTTTCGATT
  AAGCACATACCTTATACAACACCAAAAAATTCACACTGGCGAGAAGCCTTTTCTTTGTATTGAGTGTGGA
  AAAAGTTTCAGTCGGAGCTCATTCCTTATTGAACATCAGAGGATCCATACTGGTGAAAGACCTTATCAGT
  GCAAAGAGTGTGGGAAAAGTTTCAGTCAGCTTTGCAACCTTACTCGTCATCAGAGAATTCACACAGGAGA
  CAAGCCCCATAAATGTGAGGAATGTGGAAAAGCCTTTAGTAGAAGCTCAGGTCTTATTCAGCATCAGAGA
  ATTCACACCAGGGAGAAGACTTATCCATACAATGAAACTAAGGAAAGTTTTGATCCAAATTGCAGTCTTG
  TTATACAGCAGGAAGTCTACCCTAAGGAGAAATCTTATAAATGTGATGAATGTGGGAAAACTTTTAGTGT
  TAGTGCTCATCTTGTACAACATCAAAGAATCCACACTGGTGAAAAGCCCTATCTATGTACTGTCTGTGGG
  AAAAGCTTCAGCCGGAGCTCATTTCTTATTGAACATCAGAGAATCCACACTGGTGAGAGACCCTATCTGT
  GCAGACAGTGTGGAAAAAGCTTTAGTCAGCTTTGTAATCTTATTCGACATCAGGGTGTTCACACAGGTAA
  TAAACCCCATAAATGTGATGAATGTGGAAAGGCCTTTAGCCGGAACTCGGGTCTTATTCAGCATCAGAGA
  ATACACACAGGAGAGAAACCTTATAAGTGTGAGAAGTGCGACAAAAGTTTCAGTCAACAGCGCAGTCTTG
  TCAACCATCAGAAGATCCATGCAGAGGTGAAAACCCAAGAAACCCATGAATGTGACGCTTGTGGTGAAGC
  CTTTAATTGCCGTATTTCTCTTATTCAGCATCAGAAATTGCACACAGCATGGATGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217848 representing NM_003452
  Red=Cloning site Green=Tags(s)
  
  MASPSPPPESKGLLTFEDVAVFFTQEEWDYLDPAQRSLYKDVMMENYGNLVSLDVLNRDKDEEPTVKQEI
  EEIEEEVEPQGVIVTRIKSEIDQDPMGRETFELVGRLDKQRGIFLWEIPRESLTQEQRMFRENTNIIRKR
  PNSEEKCHKCEECGKGFVRKAHFIQHQRVHTGEKPFQCNECGKSFSRSSFVIEHQRIHTGERPYECNYCG
  KTFSVSSTLIRHQRIHTGERPYQCNQCKQSFSQRRSLVKHQRIHTGEKPHKCSDCGKAFSWKSHLIEHQR
  THTGEKPYHCTKCKKSFSRNSLLVEHQRIHTGERPHKCGECGKAFRLSTYLIQHQKIHTGEKPFLCIECG
  KSFSRSSFLIEHQRIHTGERPYQCKECGKSFSQLCNLTRHQRIHTGDKPHKCEECGKAFSRSSGLIQHQR
  IHTREKTYPYNETKESFDPNCSLVIQQEVYPKEKSYKCDECGKTFSVSAHLVQHQRIHTGEKPYLCTVCG
  KSFSRSSFLIEHQRIHTGERPYLCRQCGKSFSQLCNLIRHQGVHTGNKPHKCDECGKAFSRNSGLIQHQR
  IHTGEKPYKCEKCDKSFSQQRSLVNHQKIHAEVKTQETHECDACGEAFNCRISLIQHQKLHTAWMQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003452
• ORF Size: 1878 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003452.4
• RefSeq Size: 3186 bp
• RefSeq ORF: 1881 bp
• Locus ID: 7743
• UniProt ID: O75820
• Cytogenetics: 9q31.1
• Domains: KRAB, zf-C2H2
• Protein Families: Transcription Factors
• MW: 72.8 kDa
• Gene Summary: Kruppel-like zinc finger proteins such as ZNF189 contain a conserved stretch of 7 amino acids that connects a variable number of DNA-binding zinc finger repeats of the cys(2)his(2) (C2H2) type (summarized by Odeberg et al., 1998 [PubMed 9653648]). Approximately 30% of human Kruppel-like zinc finger proteins contain an N-terminal Kruppel-associated box (KRAB) domain. The KRAB domain consists of approximately 75 amino acids that may be subdivided into an A box, which is present in every KRAB domain and is essential for transcriptional repression, and a B box, which is not always present.[supplied by OMIM, May 2010]