RLTPR (CARMIL2) (NM_001013838) Human Tagged ORF Clone

CAT#: RC217662

RLTPR (Myc-DDK-tagged)-Human RGD motif, leucine rich repeats, tropomodulin domain and proline-rich containing (RLTPR)

Product Images

Western blot validation of overexpression lysate (Cat# LY423039) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217662 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RLTPR
• Synonyms: CARMIL2b; IMD58; LRRC16C; RLTPR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217662 representing NM_001013838
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGACCCCCGACGGCATCTCCTGTGAGCTCCGAGGCGAGATCACCAGGTTCCTGTGGCCCAAAG
  AGGTGGAGCTGCTGCTGAAAACCTGGCTACCCGGGGAGGGTGCTGTGCAAAACCATGTCCTGGCACTGCT
  ACGATGGAGAGCCTACCTGCTGCACACCACCTGCCTCCCGCTGAGGGTGGACTGCACGTTCAGCTACCTG
  GAGGTCCAGGCCATGGCGCTGCAGGAGACACCCCCTCAGGTCACCTTTGAGCTGGAGTCCCTGCGTGAGC
  TGGTCCTGGAGTTTCCTGGTGTGGCCGCCCTGGAACAGCTGGCCCAGCACGTGGCTGCAGCCATCAAGAA
  GGTCTTCCCTCGCTCGACCCTTGGGAAGCTATTCCGGAGGCCCACACCAGCCTCCATGCTGGCTCGGCTG
  GAGAGAAGCAGCCCCTCGGAGTCCACTGACCCCTGCAGCCCCTGTGGTGGCTTCTTGGAGACATACGAGG
  CTCTGTGTGACTACAATGGCTTCCCTTTCCGAGAGGAGATTCAGTGGGACGTGGACACCATTTACCATCG
  CCAGGGCTGCCGCCATTTCAGCCTGGGAGACTTCAGCCACCTCGGCAGTCGGGACCTGGCCTTGAGTGTG
  GCTGCCCTGTCCTACAACCTGTGGTTCCGGTGCCTCTCCTGTGTGGACATGAAGCTGAGCCTTGAGGTCT
  CAGAACAGATTCTGCACATGATGAGTCAGTCATCACACCTGGAGGAGCTGGTGCTGGAGACCTGCAGCCT
  GAGGGGAGACTTTGTCCGACGACTGGCCCAGGCGCTGGCGGGACACTCAAGCTCTGGGCTGCGGGAGCTC
  AGCCTCGCGGGGAACCTGCTGGATGACCGAGGCATGACTGCACTCAGCAGACACCTCGAGCGTTGTCCAG
  GAGCCCTGAGGAGACTCAGCCTGGCCCAGACAGGGTTGACACCGCGAGGAATGAGGGCTCTGGGCCGGGC
  ACTGGCCACCAATGCCGCCTTCGACTCCACCCTGACCCACCTGGACCTTTCTGGGAATCCTGGGGCGCTG
  GGGGCCTCCGAGGACAGTGGGGGCCTCTATAGCTTCCTGAGCCGTCCTAACGTACTGTCGTTCCTGAATC
  TCGCAGGCACCGACACTGCCCTGGACACTGTGAGGGGGTGCTCCGTGGGGGGATGGATGACCGGCAGGGC
  GGACTGGAGGGCGGGACGGGGAGGGCTCGGTCCCCCCGCGGGTGTAGCCAACAGCCTCCCCCCGCAGCTC
  TTCGCAGCGGTATCCCGAGGCTGCTGCACCAGCCTTACCCACCTCGACGCTTCGAGGAACGTCTTCTCCC
  GCACGAAGTCCCGCGCCGCGCCGGCCGCGCTGCAGCTCTTCCTCAGCCGCGCGCGGACGCTGCGGCACCT
  GGGCCTGGCGGGCTGCAAGCTGCCGCCCGACGCGCTCAGAGCCCTTTTGGATGGCCTCGCGCTCAACACG
  CACCTCCGCGACCTGCACCTGGACCTCAGCGCTTGCGAGCTGCGCTCGGCCGGCGCCCAGGTGATACAAG
  ACTTAGTGTGCGACGCAGGCGCTGTGAGCTCCCTGGATCTGGCGGATAACGGCTTCGGCTCAGACATGGT
  GACTCTGGTGCTGGCCATCGGGAGAAGCCGGTCCCTGAGACATGTGGCGCTTGGAAGGAACTTCAACGTC
  CGGTGCAAGGAGACCCTGGACGACGTCCTGCACCGGATTGTCCAGCTCATGCAGGACGACGATTGTCCTC
  TTCAGTCTCTGTCGGTGGCTGAGTCTCGGCTGAAGCTGGGTGCCAGCGTCCTACTCCGGGCCCTAGCCAC
  CAATCCTAACCTGACCGCGCTGGATATCAGCGGCAACGCCATGGGGGACGCGGGCGCCAAGTTGCTGGCC
  AAGGCGCTGCGGGTCAACTCGAGGCTCCGGTCTGTGGTCTGGGACCGGAACCACACATCTGCTTTGGGTC
  TGCTGGACGTGGCGCAGGCGCTGGAGCAGAACCACAGCCTGAAGGCCATGCCTCTGCCACTGAACGACGT
  GGCCCAGGCGCAGCGCAGCCGCCCGGAACTGACAGCACGTGCAGTCCATCAGATCCAAGCCTGTCTCTTG
  AGGAACAACCGCGCAGACCCTGCCTCTTCTGACCACACGACCCGCCTTCAGCCACTTGGTCTGGTCTCAG
  ACCCCTCAGAGCAGGAAGTGAATGAATTGTGTCAGTCGGTGCAGGAGCATGTGGAGCTGCTGGGCTGTGG
  GGCTGGGCCCCAGGGTGAAGCCGCTGTGCGCCAGGCCGAGGATGCCATCCAAAATGCCAACTTCTCTCTC
  AGCATTCTCCCCATTCTATATGAAGCTGGAAGCTCCCCAAGCCATCACTGGCAGCTTGGGCAGAAGCTGG
  AGGGCCTTCTGAGACAGGTGGGCGAGGTCTGCCGCCAGGACATCCAGGACTTCACTCAGGCCACACTGGA
  CACAGCAAGGAGCCTCTGCCCACAGATGCTGCAGGGATCCAGCTGGAGGGAGCAGCTAGAGGGGGTCCTG
  GCAGGCTCGAGGGGCCTCCCGGAGCTGCTCCCAGAGCAGCTGCTGCAAGATGCCTTCACTAGGCTCAGGG
  ACATGCGGCTATCAATCACGGGGACCTTGGCAGAGAGCATTGTGGCTCAGGCTTTGGCAGGCCTGAGTGC
  AGCCCGGGATCAGCTGGTGGAGAGTCTGGCTCAGCAGGCAACAGTGACAATGCCCCCTGCCCTACCAGCA
  CCGGATGGAGGTGAGCCCAGCCTCCTTGAGCCTGGGGAATTGGAAGGTCTTTTCTTCCCCGAGGAGAAGG
  AAGAGGAGAAGGAGAAGGATGACAGTCCTCCACAGAAATGGCCTGAGCTCAGCCACGGTCTTCACCTGGT
  CCCCTTCATTCACAGTGCTGCTGAGGAAGCGGAGCCGGAGCCCGAGCTGGCGGCTCCGGGAGAAGATGCA
  GAGCCGCAGGCGGGGCCGTCCGCGCGCGGCTCTCCGAGCCCTGCCGCCCCTGGGCCCCCGGCCGGCCCGC
  TGCCCCGCATGGACCTGCCACTGGCGGGGCAGCCCCTGCGCCATCCGACCCGGGCCCGGCCGCGGCCGCG
  CCGCCAGCACCACCACCGCCCGCCGCCGGGGGGCCCCCAGGTACCCCCAGCCTTGCCGCAGGAAGGGAAT
  GGGCTCAGTGCCCGCGTGGACGAGGGCGTGGAGGAATTCTTCTCCAAAAGGCTGATCCAGCAGGATCGCC
  TCTGGGCCCCCGAGGAGGACCCGGCCACTGAGGGGGGCGCCACTCCTGTCCCCCGTACACTGCGAAAGAA
  GCTGGGCACCCTCTTTGCCTTCAAGAAGCCTCGTTCAACGCGGGGTCCACGGACTGATCTAGAGACCAGC
  CCTGGGGCAGCTCCCCGAACCCGAAAAACTACATTTGGCGACCTACTGCGGCCGCCAACCCGTCCCAGCC
  GTGGTGAGGAGCTTGGTGGGGCTGAGGGGGACACCAGCAGCCCTGACCCTGCCGGCAGGAGCCGACCTCG
  CTACACAAGAGATAGCAAGGCCTACTCGATGATACTGCTGCCTGCCGAGGAGGAGGCAACGCTGGGTGCC
  AGACCCGACAAGCGGCGGCCCCTGGAGCGGGGAGAAACAGAACTGGCTCCATCCTTTGAACAGCGGGTAC
  AAGTAATGCTGCAGAGGATAGGCGTCAGCCGAGGCAGCGGGGGTGCCGAAGGCAAGAGGAAGCAAAGCAA
  AGATGGCGAGATCAAGAAAGCTGGCTCAGATGGTGACATTATGGACAGTTCCACGGAGGCCCCTCCCATC
  TCGATCAAGTCCCGCACCCACTCTGTGTCTGCTGACCCTTCCTGCAGACCTGGCCCAGGGAGCCAGGGGC
  CTGAGTCTGCCACCTGGAAGACACTGGGGCAGCAGTTGAATGCGGAGCTCAGGAGCCGTGGTTGGGGCCA
  ACAGGATGGTCCAGGCCCTCCCTCCCCTGGTCAAAGCCCAAGTCCCTGCAGAACCAGCCCCTCCCCAGAC
  AGCCTGGGCCTCCCAGAGGACCCTTGCTTGGGCCCCAGAAATGAAGATGGCCAGCTGAGGCCGAGGCCTC
  TCTCGGCAGGGCGGCGAGCAGTGTCTGTGCATGAGGACCAGCTCCAGGCCCCTGCTGAACGGCCCCTGAG
  GCTGCAGCGCTCCCCCGTCCTCAAACGCAGGCCAAAACTCGAGGCACCTCCATCCCCAAGCCTAGGATCT
  GGCCTTGGAACCGAGCCTCTGCCCCCACAGCCCACAGAGCCCTCCAGCCCTGAGCGGAGCCCACCCTCCC
  CAGCCACAGACCAAAGAGGCGGCGGCCCCAATCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217662 representing NM_001013838
  Red=Cloning site Green=Tags(s)
  
  MAQTPDGISCELRGEITRFLWPKEVELLLKTWLPGEGAVQNHVLALLRWRAYLLHTTCLPLRVDCTFSYL
  EVQAMALQETPPQVTFELESLRELVLEFPGVAALEQLAQHVAAAIKKVFPRSTLGKLFRRPTPASMLARL
  ERSSPSESTDPCSPCGGFLETYEALCDYNGFPFREEIQWDVDTIYHRQGCRHFSLGDFSHLGSRDLALSV
  AALSYNLWFRCLSCVDMKLSLEVSEQILHMMSQSSHLEELVLETCSLRGDFVRRLAQALAGHSSSGLREL
  SLAGNLLDDRGMTALSRHLERCPGALRRLSLAQTGLTPRGMRALGRALATNAAFDSTLTHLDLSGNPGAL
  GASEDSGGLYSFLSRPNVLSFLNLAGTDTALDTVRGCSVGGWMTGRADWRAGRGGLGPPAGVANSLPPQL
  FAAVSRGCCTSLTHLDASRNVFSRTKSRAAPAALQLFLSRARTLRHLGLAGCKLPPDALRALLDGLALNT
  HLRDLHLDLSACELRSAGAQVIQDLVCDAGAVSSLDLADNGFGSDMVTLVLAIGRSRSLRHVALGRNFNV
  RCKETLDDVLHRIVQLMQDDDCPLQSLSVAESRLKLGASVLLRALATNPNLTALDISGNAMGDAGAKLLA
  KALRVNSRLRSVVWDRNHTSALGLLDVAQALEQNHSLKAMPLPLNDVAQAQRSRPELTARAVHQIQACLL
  RNNRADPASSDHTTRLQPLGLVSDPSEQEVNELCQSVQEHVELLGCGAGPQGEAAVRQAEDAIQNANFSL
  SILPILYEAGSSPSHHWQLGQKLEGLLRQVGEVCRQDIQDFTQATLDTARSLCPQMLQGSSWREQLEGVL
  AGSRGLPELLPEQLLQDAFTRLRDMRLSITGTLAESIVAQALAGLSAARDQLVESLAQQATVTMPPALPA
  PDGGEPSLLEPGELEGLFFPEEKEEEKEKDDSPPQKWPELSHGLHLVPFIHSAAEEAEPEPELAAPGEDA
  EPQAGPSARGSPSPAAPGPPAGPLPRMDLPLAGQPLRHPTRARPRPRRQHHHRPPPGGPQVPPALPQEGN
  GLSARVDEGVEEFFSKRLIQQDRLWAPEEDPATEGGATPVPRTLRKKLGTLFAFKKPRSTRGPRTDLETS
  PGAAPRTRKTTFGDLLRPPTRPSRGEELGGAEGDTSSPDPAGRSRPRYTRDSKAYSMILLPAEEEATLGA
  RPDKRRPLERGETELAPSFEQRVQVMLQRIGVSRGSGGAEGKRKQSKDGEIKKAGSDGDIMDSSTEAPPI
  SIKSRTHSVSADPSCRPGPGSQGPESATWKTLGQQLNAELRSRGWGQQDGPGPPSPGQSPSPCRTSPSPD
  SLGLPEDPCLGPRNEDGQLRPRPLSAGRRAVSVHEDQLQAPAERPLRLQRSPVLKRRPKLEAPPSPSLGS
  GLGTEPLPPQPTEPSSPERSPPSPATDQRGGGPNP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001013838
• ORF Size: 4305 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013838.3
• RefSeq Size: 4512 bp
• RefSeq ORF: 4308 bp
• Locus ID: 146206
• UniProt ID: Q6F5E8
• Cytogenetics: 16q22.1
• MW: 154.5 kDa
• Gene Summary: This gene encodes a member of the CARMIL (capping protein, Arp2/3, myosin-I linker) family of proteins. The encoded protein interacts with and negatively regulates the heterodimeric capping protein and promotes cell migration. Reduced expression of this gene has been observed in human psoriasis patients. Mutations in this gene cause a human immunodeficiency syndrome characterized by smooth muscle tumors and impaired T-cell function. [provided by RefSeq, May 2017]