CARD10 (NM_014550) Human Tagged ORF Clone

CAT#: RC217652

CARD10 (Myc-DDK-tagged)-Human caspase recruitment domain family, member 10 (CARD10)

Product Images

Circular map for RC217652

• 

  Western blot validation of overexpression lysate (Cat# LY415227) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217652 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CARD10 protein (Cat# TP317652). The protein was produced from HEK293T cells transfected with CARD10 cDNA clone (Cat# RC217652) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CARD10
• Synonyms: BIMP1; CARMA3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217652 representing NM_014550
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGGCCGGGCGGAGGCGGGGGAGGCCGAGGAGGAGGCCGGGGCCGGCTCGGGGTCTGAGGCGGAGG
  AGGACGCGCTGTGGGAGCGAATCGAGGGCGTCCGGCATCGGCTGGCTCGCGCCCTGAACCCGGCCAAGCT
  CACGCCGTATCTGCGCCAGTGCCGGGTCATCGACGAGCAGGACGAGGAGGAGGTGCTGAGCACCTACCGC
  TTCCCGTGCCGCGTCAACCGCACCGGGCGCCTGATGGACATCTTGCGCTGCCGTGGCAAGAGGGGCTATG
  AGGCCTTCCTGGAAGCCCTGGAGTTCTACTACCCCGAACACTTCACGCTGCTCACGGGCCAGGAACCCGC
  CCAGCGCTGCTCCATGATCCTCGATGAGGAGGGGCCTGAGGGCCTGACCCAATTCTTGATGACAGAGGTG
  CGACGGCTGCGGGAAGCTCGCAAGAGCCAGCTGCAGCGGGAGCAGCAACTGCAGGCCCGGGGCCGGGTGC
  TCGAGGAGGAGCGGGCAGGGCTGGAGCAGCGGCTGCGGGACCAGCAGCAGGCTCAGGAGCGCTGTCAACG
  GCTGCGGGAGGACTGGGAGGCGGGCAGCCTGGAGCTGCTGCGGCTCAAGGATGAGAACTACATGATCGCC
  ATGCGCCTGGCACAGCTCAGTGAGGAGAAGAACTCGGCTGTACTTCGCAGCCGTGACCTGCAGCTGGCGG
  TGGATCAGCTCAAGCTCAAAGTGAGTCGGCTGGAGGAAGAGTGTGCACTGCTTCGAAGGGCCAGGGGCCC
  GCCCCCTGGGGCAGAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGAGCCAGACAATGTGGACCTTGTC
  TCTGAGCTGCGTGCTGAGAACCAGCGGCTGACGGCGTCACTGCGGGAGTTGCAGGAGGGCCTGCAGCAGG
  AGGCGAGCCGGCCGGGGGCCCCGGGCTCCGAGCGCATCCTGCTGGACATCCTAGAGCATGACTGGCGGGA
  GGCGCAGGACAGCAGGCAGGAGCTGTGCCAGAAGCTGCATGCCGTGCAGGGGGAGCTGCAGTGGGCCGAG
  GAGCTGCGCGACCAGTACCTGCAGGAGATGGAAGACCTGCGGCTCAAGCACCGCACGCTGCAGAAGGACT
  GTGACCTGTACAAGCACCGCATGGCCACTGTCCTGGCCCAACTGGAGGAGATTGAGAAGGAGCGAGACCA
  GGCCATCCAGAGCCGTGACCGGATCCAGTTGCAGTACTCACAGAGCCTCATCGAGAAGGACCAGTACCGC
  AAGCAGGTGCGGGGCCTGGAGGCGGAGCGGGATGAGCTGCTGACAACGCTCACCAGCCTGGAGGGCACCA
  AGGCTCTGCTGGAGGTTCAGCTGCAGCGGGCCCAGGGTGGCACCTGCCTCAAGGCCTGTGCCTCCTCCCA
  TTCCCTGTGCTCCAACCTCAGCAGCACTTGGAGCCTGAGCGAGTTCCCCTCCCCTCTGGGAGGCCCAGAA
  GCAACTGGGGAGGCAGCTGTCATGGGGGGACCTGAGCCTCACAACTCGGAGGAAGCCACAGACAGTGAAA
  AGGAGATCAATCGGCTCTCCATCCTGCCCTTCCCCCCCAGTGCCGGCTCCATCCTCCGCCGGCAGCGTGA
  GGAAGACCCCGCACCCCCTAAGAGATCCTTCAGCAGCATGTCAGACATCACAGGGAGTGTGACACTTAAG
  CCCTGGTCCCCTGGCCTCTCTTCGTCCTCATCCTCTGACAGCGTGTGGCCTTTGGGAAAGCCGGAAGGCC
  TCCTGGCTCGGGGCTGTGGCCTGGACTTCCTCAACAGGTCTCTGGCTATTCGGGTGTCTGGCCGGAGCCC
  CCCAGGGGGCCCAGAGCCGCAGGACAAGGGACCAGATGGACTGTCGTTTTATGGGGACAGATGGTCTGGG
  GCTGTGGTGCGCAGGGTGCTGTCTGGGCCTGGGTCCGCCAGGATGGAACCAAGAGAGCAAAGGGTGGAAG
  CTGCTGGTCTGGAGGGGGCGTGCCTGGAAGCCGAGGCCCAGCAGAGAACCTTGCTCTGGAATCAGGGGTC
  CACACTCCCCTCCCTGATGGACTCGAAGGCCTGCCAGTCCTTCCACGAGGCCCTAGAAGCCTGGGCAAAG
  GGACCAGGTGCCGAGCCCTTCTACATTCGTGCCAACCTCACCTTGCCTGAGAGGGCAGATCCCCATGCCC
  TTTGCGTGAAAGCCCAAGAGATCCTTCGACTGGTGGACTCGGCATACAAGCGGAGGCAGGAATGGTTCTG
  CACCCGGGTTGACCCCCTCACTCTGCGGGACCTGGACCGGGGCACCGTGCCCAATTATCAGAGAGCCCAG
  CAGCTCCTAGAAGTTCAGGAGAAATGCCTGCCCTCCAGCCGGCACCGAGGCCCCCGCAGTAATCTGAAGA
  AGAGAGCCCTGGACCAGCTGCGGCTGGTGAGGCCCAAGCCCGTGGGGGCGCCTGCAGGGGACTCCCCGGA
  TCAGCTGCTGCTGGAGCCCTGTGCAGAGCCGGAGCGGAGCCTCAGACCCTACAGTTTGGTGCGGCCGCTA
  CTGGTGTCTGCCCTGCGGCCCGTGGTGCTGTTGCCTGAGTGCCTGGCGCCCCGGCTCATCCGTAACCTGC
  TAGACCTGCCCAGCTCCCGGCTGGACTTCCAAGTGTGCCCAGCGGAAAGCCTCTCTGGGGAGGAACTGTG
  CCCATCCTCAGCGCCTGGAGCCCCCAAGGCTCAGCCTGCCACCCCTGGGCTAGGCAGCAGGATCCGGGCC
  ATCCAGGAGTCTGTTGGGAAGAAGCACTGCCTGCTGGAGCTGGGTGCTCGGGGTGTGCGGGAGCTGGTGC
  AGAACGAGATCTACCCCATCGTCATCCACGTGGAGGTGACTGAGAAGAATGTCCGGGAAGTCAGGGGTCT
  GCTGGGCCGGCCGGGCTGGCGGGACTCAGAGCTGCTGCGGCAGTGCCGTGGCTCAGAGCAGGTGCTCTGG
  GGGCTGCCCTGCTCCTGGGTGCAGGTGCCCGCCCATGAGTGGGGACACGCAGAGGAGCTGGCCAAGGTGG
  TGCGCGGCCGCATCCTGCAGGAGCAGGCCCGCCTCGTGTGGGTGGAGTGCGGCAGCAGCAGAGGCTGCCC
  CAGCAGCAGTGAGGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217652 representing NM_014550
  Red=Cloning site Green=Tags(s)
  
  MPGRAEAGEAEEEAGAGSGSEAEEDALWERIEGVRHRLARALNPAKLTPYLRQCRVIDEQDEEEVLSTYR
  FPCRVNRTGRLMDILRCRGKRGYEAFLEALEFYYPEHFTLLTGQEPAQRCSMILDEEGPEGLTQFLMTEV
  RRLREARKSQLQREQQLQARGRVLEEERAGLEQRLRDQQQAQERCQRLREDWEAGSLELLRLKDENYMIA
  MRLAQLSEEKNSAVLRSRDLQLAVDQLKLKVSRLEEECALLRRARGPPPGAEEKEKEKEKEKEPDNVDLV
  SELRAENQRLTASLRELQEGLQQEASRPGAPGSERILLDILEHDWREAQDSRQELCQKLHAVQGELQWAE
  ELRDQYLQEMEDLRLKHRTLQKDCDLYKHRMATVLAQLEEIEKERDQAIQSRDRIQLQYSQSLIEKDQYR
  KQVRGLEAERDELLTTLTSLEGTKALLEVQLQRAQGGTCLKACASSHSLCSNLSSTWSLSEFPSPLGGPE
  ATGEAAVMGGPEPHNSEEATDSEKEINRLSILPFPPSAGSILRRQREEDPAPPKRSFSSMSDITGSVTLK
  PWSPGLSSSSSSDSVWPLGKPEGLLARGCGLDFLNRSLAIRVSGRSPPGGPEPQDKGPDGLSFYGDRWSG
  AVVRRVLSGPGSARMEPREQRVEAAGLEGACLEAEAQQRTLLWNQGSTLPSLMDSKACQSFHEALEAWAK
  GPGAEPFYIRANLTLPERADPHALCVKAQEILRLVDSAYKRRQEWFCTRVDPLTLRDLDRGTVPNYQRAQ
  QLLEVQEKCLPSSRHRGPRSNLKKRALDQLRLVRPKPVGAPAGDSPDQLLLEPCAEPERSLRPYSLVRPL
  LVSALRPVVLLPECLAPRLIRNLLDLPSSRLDFQVCPAESLSGEELCPSSAPGAPKAQPATPGLGSRIRA
  IQESVGKKHCLLELGARGVRELVQNEIYPIVIHVEVTEKNVREVRGLLGRPGWRDSELLRQCRGSEQVLW
  GLPCSWVQVPAHEWGHAEELAKVVRGRILQEQARLVWVECGSSRGCPSSSEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014550
• ORF Size: 3096 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014550.4
• RefSeq Size: 3912 bp
• RefSeq ORF: 3099 bp
• Locus ID: 29775
• UniProt ID: Q9BWT7
• Cytogenetics: 22q13.1
• MW: 115.8 kDa
• Gene Summary: The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM, Mar 2008]