SYTL2 (NM_032943) Human Tagged ORF Clone

CAT#: RC217584

SYTL2 (Myc-DDK-tagged)-Human synaptotagmin-like 2 (SYTL2), transcript variant a

Product Images

Circular map for RC217584

• 

  Western blot validation of overexpression lysate (Cat# LY403206) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217584 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SYTL2 protein (Cat# TP317584). The protein was produced from HEK293T cells transfected with SYTL2 cDNA clone (Cat# RC217584) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SYTL2
• Synonyms: CHR11SYT; EXO4; PPP1R151; SGA72M; SLP2; SLP2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217584 representing NM_032943
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTGACTTAAGCTTCCTGACTGAAGAGGAACAAGAGGCCATCATGAAGGTTTTGCAGCGGGATGCTG
  CTCTGAAGAGGGCCGAAGAAGAGAGAGTCAGACATTTGCCTGAAAAAATTAAGGATGACCAGCAGCTGAA
  GAATATGAGTGGCCAATGGTTTTATGAAGCCAAGGCAAAAAGGCACAGGGACAAAATCCATGGCGCAGAT
  ATCATCAGAGCATCTATGAGAAAGAAGAGGCCCCAGATAGCAGCTGAGCAGAGTAAAGACAGAGAAAATG
  GGGCAAAGGAAAGCTGGGTGAATAATGTCAACAAAGATGCTTTCCTTCCTCCAGAGCTGGCTGGCGTTGT
  AGAAGAGCCAGAAGAAGATGCAGCACCAGCAAGCCCGAGTTCCAGTGTGGTAAATCCAGCTTCCAGTGTG
  ATTGATATGTCCCAGGAAAACACAAGGAAACCAAATGTGTCTCCAGAGAAGCAGAGGAAGAATCCGTTTA
  ATAGCTCCAAGTTGCCAGAAGGTCACTCGTCACAACAAACTAAAAATGAACAGTCAAAAAATGGAAGAAC
  TGGTTTATTTCAGACTTCAAAAGAGGATGAATTGTCAGAGTCAAAAGAAAAGTCAACTGTCGCAGATACT
  TCAATCCAAAAGTTAGAGAAATCAAAGCAGACTTTGCCAGGCCTTTCAAATGGGTCCCAAATCAAGGCTC
  CAATCCCCAAAGCCAGGAAGATGATCTACAAATCAACTGATTTAAACAAAGATGATAACCAGTCTTTTCC
  TAGACAAAGGACAGACTCCCTGAAAGCGAGAGGGGCTCCGAGAGGGATCCTCAAGCGCAACTCCAGTTCC
  AGTAGCACAGACTCAGAAACCCTTCGTTATAATCACAACTTTGAACCCAAAAGCAAAATTGTGTCACCTG
  GCCTAACCATCCATGAGAGAATTTCTGAGAAGGAGCATTCTTTAGAAGACAACTCTTCCCCAAACTCCCT
  GGAGCCATTAAAGCATGTGAGATTCTCTGCAGTGAAGGATGAGCTTCCACAGAGTCCTGGGCTAATCCAT
  GGTCGGGAAGTAGGAGAATTTAGTGTTTTAGAATCTGACAGATTGAAAAATGGAATGGAAGATGCAGGGG
  ACACAGAAGAGTTTCAGAGTGACCCTAAGCCTTCTCAATACAGAAAGCCTTCGCTTTTTCATCAATCAAC
  CTCAAGCCCATATGTATCAAAAAGTGAAACACATCAGCCAATGACTTCTGGTTCTTTTCCAATTAATGGG
  CTGCATTCTCATTCAGAAGTTTTAACTGCAAGACCACAGTCTATGGAGAATTCACCAACCATCAATGAAC
  CCAAAGATAAATCATCAGAATTAACAAGGCTTGAATCTGTATTACCCAGAAGCCCTGCTGATGAACTGTC
  TCATTGTGTTGAGCCTGAGCCATCTCAGGTGCCAGGTGGCAGTTCTAGAGACCGTCAGCAAGGTTCAGAA
  GAAGAACCCAGTCCTGTTTTGAAAACTTTGGAAAGGAGTGCCGCTAGGAAAATGCCTTCCAAAAGTCTAG
  AAGACATTTCATCAGATTCATCAAATCAAGCAAAAGTAGATAATCAGCCAGAAGAATTAGTGCGTAGTGC
  TGAAGATGATGAGAAACCAGATCAGAAGCCAGTTACAAATGAATGCGTACCAAGAATTTCCACAGTGCCT
  ACACAACCTGATAATCCATTTTCTCACCCTGACAAACTCAAAAGGATGAGCAAGTCTGTTCCAGCATTTC
  TCCAAGATGAGGTGAGTGGCAGTGTGATGAGTGTTTATAGTGGAGACTTTGGCAATCTGGAAGTTAAAGG
  AAATATTCAGTTTGCAATTGAATATGTGGAGTCACTGAAGGAGTTGCATGTTTTTGTGGCCCAGTGTAAG
  GACTTAGCAGCAGCGGATGTAAAAAAACAGCGTTCAGACCCATATGTAAAGGCCTATTTGCTACCAGACA
  AAGGCAAAATGGGCAAGAAGAAAACACTCGTAGTGAAGAAAACCTTGAATCCTGTGTATAACGAAATACT
  GCGGTATAAAATTGAAAAACAAATCTTAAAGACACAGAAATTGAACCTGTCCATTTGGCATCGGGATACA
  TTTAAGCGCAATAGTTTCCTAGGGGAGGTGGAACTTGATTTGGAAACATGGGACTGGGATAACAAACAGA
  ATAAACAATTGAGATGGTACCCTCTGAAGCGGAAGACAGCACCAGTTGCCCTTGAAGCAGAAAACAGAGG
  TGAAATGAAACTAGCTCTCCAGTATGTCCCAGAGCCAGTCCCTGGTAAAAAGCTTCCTACAACTGGAGAA
  GTGCACATCTGGGTGAAGGAATGCCTTGATCTACCACTGCTAAGGGGAAGTCATCTAAATTCTTTTGTTA
  AATGTACCATCCTTCCAGATACAAGTAGGAAAAGTCGCCAGAAGACAAGAGCTGTAGGGAAAACCACCAA
  CCCTATCTTCAACCACACTATGGTGTATGATGGGTTCAGGCCTGAAGATCTGATGGAAGCCTGTGTAGAG
  CTTACTGTCTGGGACCATTACAAATTAACCAACCAATTTTTGGGAGGTCTTCGTATTGGCTTTGGAACAG
  GTAAAAGTTATGGGACTGAAGTGGACTGGATGGACTCTACTTCAGAGGAAGTTGCTCTCTGGGAGAAGAT
  GGTAAACTCCCCCAATACTTGGATTGAAGCAACACTGCCTCTCAGAATGCTTTTGATTGCCAAGATTTCC
  AAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217584 representing NM_032943
  Red=Cloning site Green=Tags(s)
  
  MIDLSFLTEEEQEAIMKVLQRDAALKRAEEERVRHLPEKIKDDQQLKNMSGQWFYEAKAKRHRDKIHGAD
  IIRASMRKKRPQIAAEQSKDRENGAKESWVNNVNKDAFLPPELAGVVEEPEEDAAPASPSSSVVNPASSV
  IDMSQENTRKPNVSPEKRKNPFNSSKLPEGHSSQQTKNEQSKNGRTGLFQTSKEDELSESKEKSTVADTS
  IQKLEKSKQTLPGLSNGSQIKAPIPKARKMIYKSTDLNKDDNQSFPRQRTDSLKARGAPRGILKRNSSSS
  STDSETLRYNHNFEPKSKIVSPGLTIHERISEKEHSLEDNSSPNSLEPLKHVRFSAVKDELPQSPGLIHG
  REVGEFSVLESDRLKNGMEDAGDTEEFQSDPKPSQYRKPSLFHQSTSSPYVSKSETHQPMTSGSFPINGL
  HSHSEVLTARPQSMENSPTINEPKDKSSELTRLESVLPRSPADELSHCVEPEPSQVPGGSSRDRQQGSEE
  EPSPVLKTLERSAARKMPSKSLEDISSDSSNQAKVDNQPEELVRSAEDDEKPDQKPVTNECVPRISTVPT
  QPDNPFSHPDKLKRMSKSVPAFLQDEVSGSVMSVYSGDFGNLEVKGNIQFAIEYVESLKELHVFVAQCKD
  LAAADVKKQRSDPYVKAYLLPDKGKMGKKKTLVVKKTLNPVYNEILRYKIEKQILKTQKLNLSIWHRDTF
  KRNSFLGEVELDLETWDWDNKQNKQLRWYPLKRKTAPVALEAENRGEMKLALQYVPEPVPGKKLPTTGEV
  HIWVKECLDLPLLRGSHLNSFVKCTILPDTSRKSRQKTRAVGKTTNPIFNHTMVYDGFRPEDLMEACVEL
  TVWDHYKLTNQFLGGLRIGFGTGKSYGTEVDWMDSTSEEVALWEKMVNSPNTWIEATLPLRMLLIAKISK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032943
• ORF Size: 2733 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032943.4
• RefSeq Size: 3985 bp
• RefSeq ORF: 2733 bp
• Locus ID: 54843
• UniProt ID: Q9HCH5
• Cytogenetics: 11q14.1
• Domains: C2
• MW: 102.4 kDa
• Gene Summary: The protein encoded by this gene is a synaptotagmin-like protein (SLP) that belongs to a C2 domain-containing protein family. The SLP homology domain (SHD) of this protein has been shown to specifically bind the GTP-bound form of Ras-related protein Rab-27A (RAB27A). This protein plays a role in RAB27A-dependent vesicle trafficking and controls melanosome distribution in the cell periphery. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jun 2009]