ARHGAP28 (NM_030672) Human Tagged ORF Clone

CAT#: RC217562

ARHGAP28 (Myc-DDK-tagged)-Human Rho GTPase activating protein 28 (ARHGAP28), transcript variant 2

Product Images

Circular map for RC217562

• 

  Western blot validation of overexpression lysate (Cat# LY410740) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217562 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARHGAP28
• Synonyms: DKFZp686A2038; FLJ10312; Rho GTPase activating protein 28
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217562 representing NM_030672
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAAAAAGGATAAGCAATCTATCAGGGATGTCAGAGACATTTTTGGAGTCAGTGAATCTCCTCCTC
  GTGATACCTGTGGCAACCACACTAATCAGCTGGATGGCACCAAGGAAGAAAGAGAGCTTCCAAGAGTTAT
  CAAGACAAGTGGTTCCATGCCAGATGATGCTTCTCTCAACAGTACTACCCTGTCTGACGCATCCCAGGAT
  AAAGAAGGGAGTTTTGCGGTTCCCAGGAGTGACTCTGTGGCTATACTTGAGACCATTCCAGTTCTACCAG
  TTCATTCCAATGGATCACCGGAGCCTGGACAGCCAGTTCAGAATGCGATAAGTGATGATGATTTTCTGGA
  AAAGAACATTCCACCAGAGGCTGAAGAGCTGTCATTTGAAGTGTCTTATTCAGAAATGGTTACGGAGGCT
  CTAAAAAGAAATAAACTTAAGAAATCAGAGATTAAGAAAGAAGACTATGTTTTAACTAAATTTAATGTTC
  AGAAAACCAGATTTGGCTTAACTGAAGCAGGAGATCTGTCTGCTGAAGACATGAAGAAAATCCGCCATCT
  CTCTCTGATTGAATTGACTGCCTTTTTTGATGCCTTTGGAATTCAACTGAAAAGGAACAAAACAGAGAAA
  GTAAAAGGACGAGACAATGGGATTTTTGGAGTTCCACTTACAGTCCTCCTGGACGGTGACCGAAAGAAAG
  ACCCTGGAGTGAAAGTTCCCCTGGTATTACAAAAATTTTTTGAGAAAGTTGAGGAATCAGGTCTGGAATC
  TGAAGGAATTTTTCGACTTTCAGGATGTACTGCTAAAGTCAAGCAATACCGTGAAGAACTTGATGCCAAG
  TTTAATGCTGATAAATTTAAATGGGACAAAATGTGCCATAGAGAAGCTGCAGTAATGTTGAAAGCGTTTT
  TCAGAGAACTACCCACCTCTCTCTTCCCTGTGGAATATATACCTGCCTTCATCAGTCTAATGGAAAGAGG
  GCCTCACGTCAAAGTACAGTTTCAAGCCTTACACCTCATGGTCATGGCGCTGCCTGATGCCAACAGAGAT
  GCAGCTCAGGCCCTCATGACATTCTTCAATAAAGTGATTGCCAATGAATCAAAAAACCGAATGAGTCTGT
  GGAACATTTCTACAGTGATGGCACCAAACCTTTTCTTCAGTAGAAGCAAACACTCTGATTATGAAGAATT
  ACTGTTAGCAAACACTGCGGCCCACATCATCCGCCTAATGCTTAAGTACCAGAAGATTTTGTGGAAGGTT
  CCATCTTTCTTAATCACTCAAGTAAGAAGAATGAATGAAGCCACGATGCTATTGAAGAAGCAGCTCCCAA
  GTGTCAGGAAGCTGCTCAGGAGGAAGACCCTCGAGCGGGAGACTGCAAGCCCCAAGACTTCAAAGGTACT
  GCAAAAATCACCCTCGGCAAGACGAATGTCTGACGTGCCGGAAGGAGTCATACGGGTCCATGCTCCACTT
  CTCTCCAAGGTGTCCATGGCCATTCAACTCAACAATCAAACCAAAGCCAAAGACATATTGGCAAAATTTC
  AATATGAAAACAGAATACTGCACTGGCAGCGAGCTGCACTTTCCTTCCTAAATGGAAAATGGGTTAAAAA
  AGAAAGAGAAGAGTCGACAGAGACCAACAGGAGCCCCAAACATGTATTCCTCTTCACTATTGGCCTGGAC
  ATCTCCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217562 representing NM_030672
  Red=Cloning site Green=Tags(s)
  
  MRKKDKQSIRDVRDIFGVSESPPRDTCGNHTNQLDGTKEERELPRVIKTSGSMPDDASLNSTTLSDASQD
  KEGSFAVPRSDSVAILETIPVLPVHSNGSPEPGQPVQNAISDDDFLEKNIPPEAEELSFEVSYSEMVTEA
  LKRNKLKKSEIKKEDYVLTKFNVQKTRFGLTEAGDLSAEDMKKIRHLSLIELTAFFDAFGIQLKRNKTEK
  VKGRDNGIFGVPLTVLLDGDRKKDPGVKVPLVLQKFFEKVEESGLESEGIFRLSGCTAKVKQYREELDAK
  FNADKFKWDKMCHREAAVMLKAFFRELPTSLFPVEYIPAFISLMERGPHVKVQFQALHLMVMALPDANRD
  AAQALMTFFNKVIANESKNRMSLWNISTVMAPNLFFSRSKHSDYEELLLANTAAHIIRLMLKYQKILWKV
  PSFLITQVRRMNEATMLLKKQLPSVRKLLRRKTLERETASPKTSKVLQKSPSARRMSDVPEGVIRVHAPL
  LSKVSMAIQLNNQTKAKDILAKFQYENRILHWQRAALSFLNGKWVKKEREESTETNRSPKHVFLFTIGLD
  IST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030672
• ORF Size: 1689 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030672.2, NP_109597.2
• RefSeq Size: 2059 bp
• RefSeq ORF: 1691 bp
• Locus ID: 79822
• Cytogenetics: 18p11.31
• MW: 63.9 kDa