PPTC7 (NM_139283) Human Tagged ORF Clone

CAT#: RC217495

PPTC7 (Myc-DDK-tagged)-Human PTC7 protein phosphatase homolog (S. cerevisiae) (PPTC7)

Product Images

Circular map for RC217495

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  Western blot validation of overexpression lysate (Cat# LY408339) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217495 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPTC7
• Synonyms: TA-PP2C; TAPP2C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217495 representing NM_139283
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTCGGTCCTCTCGTACGGGCGGCTGGTGGCCCGCGCCGTGCTCGGCGGCCTCTCGCAGACCGACC
  CCAGGGCCGGCGGCGGCGGCGGCGGCGACTACGGACTGGTGACGGCCGGCTGCGGCTTCGGGAAGGACTT
  CCGTAAGGGCCTCCTCAAGAAGGGCGCGTGCTACGGGGACGACGCGTGCTTCGTGGCCCGGCACCGTTCC
  GCGGACGTGCTCGGGGTTGCAGATGGTGTAGGAGGCTGGAGAGACTATGGAGTTGATCCATCTCAATTCT
  CAGGGACTTTAATGCGGACGTGTGAACGTTTAGTAAAAGAAGGACGGTTCGTACCTAGTAATCCCATTGG
  AATTCTCACCACAAGCTACTGTGAGTTGCTGCAAAATAAAGTCCCTTTGCTCGGTAGCAGCACCGCCTGC
  ATTGTGGTGCTGGACAGAACCAGCCACCGCTTACACACAGCAAACCTGGGCGATTCAGGCTTCCTGGTTG
  TCAGGGGTGGTGAAGTCGTGCACCGATCAGATGAGCAGCAGCATTACTTCAACACTCCATTCCAGCTCTC
  AATCGCTCCCCCTGAAGCCGAGGGAGTCGTCTTGAGCGACAGTCCGGATGCTGCTGATAGCACGTCTTTC
  GATGTCCAGCTAGGAGACATTATCCTGACGGCAACAGATGGACTCTTTGACAACATGCCTGATTATATGA
  TTCTTCAGGAGCTAAAAAAGTTAAAGAATTCAAATTATGAGAGTATACAACAGACTGCCAGAAGCATTGC
  TGAGCAAGCTCATGAGCTGGCCTATGACCCAAATTATATGTCACCTTTTGCACAGTTTGCATGTGACAAT
  GGATTGAATGTGAGAGGTGGAAAGCCAGATGACATCACCGTCCTTCTTTCAATAGTGGCTGAGTATACAG
  AC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217495 representing NM_139283
  Red=Cloning site Green=Tags(s)
  
  MFSVLSYGRLVARAVLGGLSQTDPRAGGGGGGDYGLVTAGCGFGKDFRKGLLKKGACYGDDACFVARHRS
  ADVLGVADGVGGWRDYGVDPSQFSGTLMRTCERLVKEGRFVPSNPIGILTTSYCELLQNKVPLLGSSTAC
  IVVLDRTSHRLHTANLGDSGFLVVRGGEVVHRSDEQQHYFNTPFQLSIAPPEAEGVVLSDSPDAADSTSF
  DVQLGDIILTATDGLFDNMPDYMILQELKKLKNSNYESIQQTARSIAEQAHELAYDPNYMSPFAQFACDN
  GLNVRGGKPDDITVLLSIVAEYTD
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139283
• ORF Size: 912 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139283.2
• RefSeq Size: 3748 bp
• RefSeq ORF: 915 bp
• Locus ID: 160760
• UniProt ID: Q8NI37
• Cytogenetics: 12q24.11
• Protein Families: Druggable Genome, Phosphatase
• MW: 32.5 kDa
• Gene Summary: Protein phosphatase which positively regulates biosynthesis of the ubiquinone, coenzyme Q (PubMed:30267671). Dephosphorylates the ubiquinone biosynthesis protein COQ7 which is likely to lead to its activation (PubMed:30267671).[UniProtKB/Swiss-Prot Function]