cadherin 10 (CDH10) (NM_006727) Human Tagged ORF Clone

CAT#: RC217458

CDH10 (Myc-DDK-tagged)-Human cadherin 10, type 2 (T2-cadherin) (CDH10)

Product Images

Circular map for RC217458

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  Western blot validation of overexpression lysate (Cat# LY416465) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217458 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: cadherin 10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217458 representing NM_006727
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAATACATCAATTTTTGCTACTGTTTCTATTCTGGGTATGCCTGCCACATTTCTGCTCTCCAGAAA
  TAATGTTCAGAAGGACGCCTGTGCCACAGCAAAGAATTTTAAGTTCACGTGTACCAAGGAGTGATGGCAA
  AATTCTCCATCGTCAAAAACGTGGTTGGATGTGGAATCAATTTTTCTTACTTGAAGAATATACAGGATCT
  GATTATCAGTACGTAGGCAAGCTACATTCAGACCAAGATAAAGGAGATGGATCACTCAAATATATCTTAT
  CTGGAGATGGAGCTGGTACTCTTTTTATTATTGATGAAAAAACAGGTGATATTCATGCCACAAGGCGAAT
  TGATAGGGAGGAAAAGGCCTTTTATACTCTACGCGCACAAGCTATTAACAGAAGAACTCTGAGGCCAGTA
  GAGCCAGAGTCAGAGTTTGTGATCAAAATTCATGATATCAATGACAATGAGCCAACGTTCCCAGAAGAAA
  TCTATACAGCTAGTGTTCCCGAAATGTCTGTTGTAGGTACTTCTGTGGTGCAAGTCACAGCTACAGATGC
  CGATGACCCTTCATATGGGAACAGCGCCAGAGTCATTTACAGCATACTTCAAGGGCAGCCCTATTTCTCT
  GTGGAGCCTGAAACAGGTATCATCAGGACTGCTTTACCGAACATGAACAGAGAAAACAGAGAGCAATACC
  AAGTGGTCATCCAGGCCAAAGACATGGGCGGCCAGATGGGAGGCTTATCGGGGACAACCACTGTGAACAT
  CACGCTGACAGATGTCAATGACAACCCACCACGTTTCCCCCAGAACACTATTCATCTTCGAGTTCTTGAA
  TCCTCCCCAGTTGGCACAGCCATTGGAAGTGTCAAAGCAACTGATGCTGACACTGGGAAAAATGCTGAAG
  TAGAATACCGAATTATTGATGGTGACGGTACTGATATGTTTGACATCGTGACTGAGAAGGACACACAGGA
  AGGCATCATCACTGTGAAAAAGCCACTCGACTATGAGAGCCGAAGACTTTATACTCTGAAAGTCGAAGCA
  GAAAACACCCATGTAGATCCCCGTTTTTATTACCTAGGACCATTTAAAGATACTACCATAGTGAAAATCT
  CTATAGAAGATGTGGATGAACCTCCTGTTTTTAGTAGGTCCTCCTATCTGTTTGAAGTTCATGAAGATAT
  TGAAGTGGGCACAATCATTGGTACTGTAATGGCAAGGGACCCAGATTCTATTTCCAGCCCCATTAGATTT
  TCCTTGGATCGCCATACTGACCTTGACAGAATCTTTAACATTCATTCAGGAAATGGATCTCTTTATACAT
  CAAAACCTCTTGACCGTGAACTATCTCAGTGGCATAATCTTACTGTTATTGCTGCTGAAATCAACAATCC
  CAAAGAGACAACACGCGTGGCTGTTTTTGTGAGAATTTTGGATGTTAATGACAATGCCCCACAGTTTGCT
  GTGTTCTATGACACTTTTGTATGTGAAAATGCCAGACCAGGGCAGCTAATACAGACTATAAGTGCAGTAG
  ACAAAGATGACCCTTTAGGTGGACAGAAATTTTTTTTCAGTTTAGCTGCTGTCAATCCAAACTTCACAGT
  ACAGGATAATGAAGATAATACTGCCAGAATCTTAACCAGAAAAAATGGATTCAATAGACATGAAATCAGT
  ACCTATCTCTTGCCTGTGGTGATATCAGACAATGATTACCCAATTCAGAGCAGCACAGGCACACTGACCA
  TTCGAGTGTGTGCTTGTGACAGCCAAGGCAACATGCAATCCTGCAGTGCTGAAGCCCTGCTCCTCCCTGC
  CGGCCTCAGCACTGGGGCCTTGATCGCCATCCTCCTCTGCATCATCATTCTACTGGTTATAGTAGTACTG
  TTTGCAGCTCTGAAAAGACAGCGAAAAAAAGAGCCTCTGATCTTGTCAAAAGAAGATATCAGAGACAACA
  TTGTGAGCTATAACGATGAGGGTGGTGGAGAGGAGGACACCCAGGCCTTTGATATCGGCACCCTGAGGAA
  TCCTGCAGCCATTGAGGAAAAAAAGCTCCGGCGAGATATTATTCCAGAAACGTTATTTATTCCTCGGAGG
  ACTCCTACAGCTCCAGATAACACGGACGTCCGGGATTTCATTAATGAAAGGCTAAAAGAGCATGATCTTG
  ACCCCACCGCACCCCCCTACGACTCACTTGCAACCTATGCCTATGAAGGAAATGATTCCATTGCTGAATC
  TCTGAGTTCATTAGAATCAGGTACTACTGAAGGAGACCAAAACTACGATTACCTCCGAGAATGGGGCCCT
  CGGTTTAATAAGCTAGCAGAAATGTATGGTGGTGGGGAAAGTGACAAAGACTCT
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217458 representing NM_006727
  Red=Cloning site Green=Tags(s)
  
  MTIHQFLLLFLFWVCLPHFCSPEIMFRRTPVPQQRILSSRVPRSDGKILHRQKRGWMWNQFFLLEEYTGS
  DYQYVGKLHSDQDKGDGSLKYILSGDGAGTLFIIDEKTGDIHATRRIDREEKAFYTLRAQAINRRTLRPV
  EPESEFVIKIHDINDNEPTFPEEIYTASVPEMSVVGTSVVQVTATDADDPSYGNSARVIYSILQGQPYFS
  VEPETGIIRTALPNMNRENREQYQVVIQAKDMGGQMGGLSGTTTVNITLTDVNDNPPRFPQNTIHLRVLE
  SSPVGTAIGSVKATDADTGKNAEVEYRIIDGDGTDMFDIVTEKDTQEGIITVKKPLDYESRRLYTLKVEA
  ENTHVDPRFYYLGPFKDTTIVKISIEDVDEPPVFSRSSYLFEVHEDIEVGTIIGTVMARDPDSISSPIRF
  SLDRHTDLDRIFNIHSGNGSLYTSKPLDRELSQWHNLTVIAAEINNPKETTRVAVFVRILDVNDNAPQFA
  VFYDTFVCENARPGQLIQTISAVDKDDPLGGQKFFFSLAAVNPNFTVQDNEDNTARILTRKNGFNRHEIS
  TYLLPVVISDNDYPIQSSTGTLTIRVCACDSQGNMQSCSAEALLLPAGLSTGALIAILLCIIILLVIVVL
  FAALKRQRKKEPLILSKEDIRDNIVSYNDEGGGEEDTQAFDIGTLRNPAAIEEKKLRRDIIPETLFIPRR
  TPTAPDNTDVRDFINERLKEHDLDPTAPPYDSLATYAYEGNDSIAESLSSLESGTTEGDQNYDYLREWGP
  RFNKLAEMYGGGESDKDS
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006727
• ORF Size: 2364 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006727.5
• RefSeq Size: 3261 bp
• RefSeq ORF: 2367 bp
• Locus ID: 1008
• UniProt ID: Q9Y6N8
• Cytogenetics: 5p14.2-p14.1
• Domains: Cadherin_C_term, CA
• Protein Families: Transmembrane
• MW: 88.3 kDa
• Gene Summary: This gene encodes a type II classical cadherin of the cadherin superfamily. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature cadherin protein. These integral membrane proteins mediate calcium-dependent cell-cell adhesion and are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a histidine-alanine-valine (HAV) cell adhesion recognition sequence specific to type I cadherins. This particular cadherin is predominantly expressed in brain and is putatively involved in synaptic adhesions, axon outgrowth and guidance. Mutations in this gene may be associated with lung squamous cell carcinoma and colorectal cancer in human patients. [provided by RefSeq, Nov 2015]