Bile Acid Receptor (NR1H4) (NM_005123) Human Tagged ORF Clone
NR1H4 (Myc-DDK-tagged)-Human nuclear receptor subfamily 1, group H, member 4 (NR1H4), transcript variant 2
|Type||Human Tagged ORF Clone|
|Synonyms||BAR; FXR; HRR-1; HRR1; PFIC5; RIP14|
|E. coli Selection||Kanamycin (25 ug/mL)|
|Mammalian Cell Selection||Neomycin|
>RC217443 representing NM_005123
Red=Cloning site Blue=ORF Green=Tags(s)
>RC217443 representing NM_005123
Red=Cloning site Green=Tags(s)
Sequencher program is needed, download here.
|Restriction Sites||SgfI-MluI Cloning Scheme for this gene Plasmid Map|
|ORF Size||1416 bp|
|OTI Disclaimer||Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at firstname.lastname@example.org or by calling 301.340.3188 option 3 for pricing and delivery.
The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
|OTI Annotation||This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.|
|Product Components||The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).|
|Reconstitution||1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
|RefSeq Size||2218 bp|
|RefSeq ORF||1419 bp|
|Protein Families||Druggable Genome, Nuclear Hormone Receptor, Transcription Factors|
|Gene Summary||This gene encodes a ligand-activated transcription factor that shares structural features in common with nuclear hormone receptor family members. This protein functions as a receptor for bile acids, and when bound to bile acids, binds to DNA and regulates the expression of genes involved in bile acid synthesis and transport. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Feb 2016]|
|cDNA Clone Resources|
|RC217443L1||Lenti ORF clone of Human nuclear receptor subfamily 1, group H, member 4 (NR1H4), transcript variant 2, Myc-DDK-tagged||
|RC217443L2||Lenti ORF clone of Human nuclear receptor subfamily 1, group H, member 4 (NR1H4), transcript variant 2, mGFP tagged||
|RC217443L3||Lenti ORF clone of Human nuclear receptor subfamily 1, group H, member 4 (NR1H4), transcript variant 2, Myc-DDK-tagged||
|RC217443L4||Lenti ORF clone of Human nuclear receptor subfamily 1, group H, member 4 (NR1H4), transcript variant 2, mGFP tagged||
|RG217443||NR1H4 (GFP-tagged) - Human nuclear receptor subfamily 1, group H, member 4 (NR1H4), transcript variant 2||
|SC116941||NR1H4 (untagged)-Human nuclear receptor subfamily 1, group H, member 4 (NR1H4), transcript variant 2||