Bile Acid Receptor (NR1H4) (NM_005123) Human Untagged Clone

CAT#: SC116941

NR1H4 (untagged)-Human nuclear receptor subfamily 1, group H, member 4 (NR1H4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Bile Acid Receptor
• Synonyms: BAR; FXR; HRR-1; HRR1; PFIC5; RIP14
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005123 edited
  TAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTT
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGAACTCTCTCCTCCTC
  CTCACCTCATTGTCTCCCCGACTTATCCTAATGCGAAATTGGATTCTGAGCATTTGTAGC
  AAAATCGCTGGGATCTGGAGAGGAAGACTCAGTCCAGAATCCTCCCAGGGCCTTGAAAGT
  CCATCTCTGACCCAAAACAATCCAAGGAGGTAGAAGACATCGTAGAAGGAGTGAAAGAAG
  AAAAGAAGACTTAGAAACATAGCTCAAAGTGAACACTGCTTCTCTTAGTTTCCTGGATTT
  CTTCTGGACATTTCCTCAAGATGAAACTTCAGACACTTTGGAGTTTTTTTTGAAGACCAC
  CATAAAGAAAGTGCATTTCAATTGAAAAATTTGGATGGGATCAAAAATGAATCTCATTGA
  ACATTCCCATTTACCTACCACAGATGAATTTTCTTTTTCTGAAAATTTATTTGGTGTTTT
  AACAGAACAAGTGGCAGGTCCTCTGGGACAGAACCTGGAAGTGGAACCATACTCGCAATA
  CAGCAATGTTCAGTTTCCCCAAGTTCAACCACAGATTTCCTCGTCATCCTATTATTCCAA
  CCTGGGTTTCTACCCCCAGCAGCCTGAAGAGTGGTACTCTCCTGGAATATATGAACTCAG
  GCGTATGCCAGCTGAGACTCTCTACCAGGGAGAAACTGAGGTAGCAGAGATGCCTGTAAC
  AAAGAAGCCCCGCATGGGCGCGTCAGCAGGGAGGATCAAAGGGGATGAGCTGTGTGTTGT
  TTGTGGAGACAGAGCCTCTGGATACCACTATAATGCACTGACCTGTGAGGGGTGTAAAGG
  TTTCTTCAGGAGAAGCATTACCAAAAACGCTGTGTACAAGTGTAAAAACGGGGGCAACTG
  TGTGATGGATATGTACATGCGAAGAAAGTGTCAAGAGTGTCGACTAAGGAAATGCAAAGA
  GATGGGAATGTTGGCTGAATGCTTGTTAACTGAAATTCAGTGTAAATCTAAGCGACTGAG
  AAAAAATGTGAAGCAGCATGCAGATCAGACCGTGAATGAAGACAGTGAAGGTCGTGACTT
  GCGACAAGTGACCTCGACAACAAAGTCATGCAGGGAGAAAACTGAACTCACCCCAGATCA
  ACAGACTCTTCTACATTTTATTATGGATTCATATAACAAACAGAGGATGCCTCAGGAAAT
  AACAAATAAAATTTTAAAAGAAGAATTCAGTGCAGAAGAAAATTTTCTCATTTTGACGGA
  AATGGCAACCAATCATGTACAGGTTCTTGTAGAATTCACAAAAAAGCTACCAGGATTTCA
  GACTTTGGACCATGAAGACCAGATTGCTTTGCTGAAAGGGTCTGCGGTTGAAGCTATGTT
  CCTTCGTTCAGCTGAGATTTTCAATAAGAAACTTCCGTCTGGGCATTCTGACCTATTGGA
  AGAAAGAATTCGAAATAGTGGTATCTCTGATGAATATATAACACCTATGTTTAGTTTTTA
  TAAAAGTATTGGGGAACTGAAAATGACTCAAGAGGAGTATGCTCTGCTTACAGCAATTGT
  TATCCTGTCTCCAGATAGACAATACATAAAGGATAGAGAGGCAGTAGAGAAGCTTCAGGA
  GCCACTTCTTGATGTGCTACAAAAGTTGTGTAAGATTCACCAGCCTGAAAATCCTCAACA
  CTTTGCCTGTCTCCTGGGTCGCCTGACTGAATTACGGACATTCAATCATCACCACGCTGA
  GATGCTGATGTCATGGAGAGTAAACGACCACAAGTTTACCCCACTTCTCTGTGAAATCTG
  GGACGTGCAGTGATGGGGATTACAGGGGAGGGGTCTAGCTCCTTTTTCTCTCTCATATTA
  ATCTGATGTATAACTTTCCTTTATTTCACTTGTACCCAGTTTCACTCAAGAAATCTTGAT
  GAATATTTATGTTGTAATTACATGTGTAACTTCCACAACTGTAAATATTGGGCTAGATAG
  AACAACTTTCTCTACATTGTGTTTTAAAAGGCTCCAGGGAATCCTGCATTCTAATTGGCA
  AGCCCTGTTTGCCTAATTAAATTGATTGTTACTTCAATTCTATCTGTTGAACTAGGGAAA
  ATCTCATTTTGCTCATCTTACCATATTGCATATATTTTATTAAAGAGTTGTATTCAATCT
  TGGC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005123 unedited
  CACCAGGNACTCTCTCCTCCTCCTCACCTCATTGTCTCCCCGACTTATCCTAATGCGAAA
  TTGGATTCTGAGCATTTGTAGCAAAATCGCTGGGATCTGGAGAGGAAGACTCAGTCCAGA
  ATCCTCCCAGGGCCTTGAAAGTCCATCTCTGACCCAAAACAATCCAAGGAGGTAGAAGAC
  ATCGTAGAAGGAGTGAAAGAAGAAAAGAAGACTTAGAAACATAGCTCAAAGTGAACACTG
  CTTCTCTTAGTTTCCTGGATTTCTTCTGGACATTTCCTCAAGATGAAACTTCAGACACTT
  TGGAGTTTTTTTTGAAGACCACCATAAAGAAAGTGCATTTCAATTGAAAAATTTGGATGG
  GATCAAAAATGAATCTCATTGAACATTCCCATTTACCTACCACAGATGAATTTTCTTTTT
  CTGAAAATTTATTTGGTGTTTTAACAGAACAAGTGGCAGGTCCTCTGGGACAGAACCTGG
  AAGTGGAACCATACTCGCAATACAGCAATGTTCAGTTTCCCCAAGTTCAACCACAGATTT
  CCTCGTCATCCTATTATTCCAACCTGNGTTTCTACCCCCAGCAGCCTGAAGAGTGGTACT
  CTCCTGNAATATATGAACTCANGCGTATGCCAGCTGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005123 unedited
  TATCTCTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTGCCATTATGTTTGCTTTATTGCCAAAATTGAATACAACTCTTTAATAAAATATATGC
  AATATGGTAAGATGAGCAAAATGAGATTTTCCCTAGTTCAACAGATAGAATTGAAGTAAC
  AATCAATTTAATTAGGCAAACAGGGCTTGCCAATTAAAATGCAGGATTCCCTGGAGCCTT
  TTAAAACACAATGTAAAGAAAGTTGTTCTATCTAGCCCAATATTTACAGTTGTGGAAGTT
  ACACATGTAATTACAACATAAATATTCATCAAGATTTCTTGAGTGAAACTGGGTACAAGT
  GAAATAAAGGAAAGTTATACATCAGATTAATATGAGAGAGAAAAAGGAGCTAGACCCCTC
  CCCTGTAATCCCCATCACTGCACGTCCCAGATTTCACAGAGAAGTGGGGTAAACTTGTGG
  TCGTTTACTCTCCATGACATCAGCATCTCAGCGTGGTGATGATTGAATGTCCGTAATTCA
  GTCAGGCGACCCAGGAGACAGGCAAAGTGTTGAGGATTTTCAGGCTGGTGAATCTTACAC
  AACTTTTGTAGCACATCAAGAAGTGGCTCCTGAAGCTTCTCTACTGCCTCTCTATCCTTT
  ATGTATTGGCTATCTGGAGACAGGATAACAATTGCTGNTAGCAGAGCATACTNCTCTTGA
  GTCATTTTCAGTTCCCCAATACTTTTATAAAAACTAAACATANNNGTGTATATATTCATC
  AGAGATACCACTATTTTCGAATTCTTTCTTNCCATAGGTCAGAATGCCCAGACGGAAAGT
  TTCTTATTGAAATCTCAGCTGAACGAAGGAACATAGCTCAACCGCAGACCCTTTCAGCAA
  GCATCTGGGCTTCATGGNCCAAGTCTGAAATCTGGAGCTTTTTGGGGATCTACAGAACTG
  ACATGGATGGTGCCATTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005123
• Insert Size: 2240 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005123.1, NP_005114.1
• RefSeq Size: 2218 bp
• RefSeq ORF: 1419 bp
• Locus ID: 9971
• UniProt ID: Q96RI1
• Cytogenetics: 12q23.1
• Domains: HOLI, zf-C4
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Gene Summary: This gene encodes a ligand-activated transcription factor that shares structural features in common with nuclear hormone receptor family members. This protein functions as a receptor for bile acids, and when bound to bile acids, binds to DNA and regulates the expression of genes involved in bile acid synthesis and transport. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. This results in a shorter isoform (2, also known as FXRalpha-) compared to isoform 1.