USP42 (NM_032172) Human Tagged ORF Clone

CAT#: RC217405

USP42 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 42 (USP42)

Product Images

Circular map for RC217405

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: USP42
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217405 representing NM_032172
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCATAGTTGACAAAGCTTCTGAATCTTCAGACCCATCAGCCTATCAGAATCAGCCTGGCAGCTCCG
  AGGCAGTCTCACCTGGAGACATGGATGCAGGTTCTGCCAGCTGGGGTGCTGTGTCTTCATTGAATGATGT
  GTCAAATCACACACTTTCTTTAGGACCAGTACCTGGTGCTGTAGTTTATTCGAGTTCATCTGTACCTGAT
  AAATCAAAACCATCACCACAAAAGGATCAAGCCCTAGGTGATGGCATCGCTCCTCCACAGAAAGTTCTTT
  TCCCATCTGAGAAGATTTGTCTTAAGTGGCAACAAACTCATAGAGTTGGAGCTGGGCTCCAGAATTTGGG
  CAATACCTGTTTTGCCAATGCAGCACTGCAGTGTTTAACCTACACACCACCTCTTGCCAATTACATGCTA
  TCACATGAACACTCCAAAACATGTCATGCAGAAGGCTTTTGTATGATGTGTACAATGCAAGCACATATTA
  CCCAGGCACTCAGTAATCCTGGGGACGTTATTAAACCAATGTTTGTCATCAATGAGATGCGGCGTATAGC
  TAGGCACTTCCGTTTTGGAAACCAAGAAGATGCCCATGAATTCCTTCAATACACTGTTGATGCTATGCAG
  AAAGCATGCTTGAATGGCAGCAATAAATTAGACAGACACACCCAGGCCACCACTCTTGTTTGTCAGATAT
  TTGGAGGATACCTAAGATCTAGAGTCAAATGTTTAAATTGCAAGGGCGTTTCAGATACTTTTGATCCATA
  TCTTGATATAACATTGGAGATAAAGGCTGCTCAGAGTGTCAACAAGGCATTGGAGCAGTTTGTGAAGCCG
  GAACAGCTTGATGGAGAAAACTCGTACAAGTGCAGCAAGTGTAAAAAGATGGTTCCAGCTTCAAAGAGGT
  TCACTATCCATAGATCCTCTAATGTTCTTACACTTTCTCTGAAACGTTTTGCAAATTTTACCGGTGGAAA
  AATTGCTAAGGATGTGAAATACCCTGAGTATCTTGATATTCGGCCATATATGTCTCAACCCAACGGAGAG
  CCAATTGTCTACGTCTTGTATGCAGTGCTGGTCCACACTGGTTTTAATTGCCATGCTGGCCATTACTTCT
  GCTACATAAAAGCTAGCAATGGCCTCTGGTATCAAATGAATGACTCCATTGTATCTACCAGTGATATTAG
  ATCGGTACTCAGCCAACAAGCCTATGTGCTCTTTTATATCAGGTCCCATGATGTGAAAAATGGAGGTGAA
  CTTACTCATCCCACCCATAGCCCCGGCCAGTCCTCTCCCCGCCCCGTCATCAGTCAGCGGGTTGTCACCA
  ACAAACAGGCTGCGCCAGGCTTTATCGGACCACAGCTTCCCTCTCACATGATAAAGAATCCACCTCACTT
  AAATGGGACTGGACCATTGAAAGACACGCCAAGCAGTTCCATGTCGAGTCCTAACGGGAATTCCAGTGTC
  AACAGGGCTAGTCCTGTTAATGCTTCAGCTTCTGTCCAAAACTGGTCAGTTAATAGGTCCTCAGTGATCC
  CAGAACATCCTAAGAAACAAAAAATTACAATCAGTATTCACAACAAGTTGCCTGTTCGCCAGTGTCAGTC
  TCAACCTAACCTTCATAGTAATTCTTTGGAGAACCCTACCAAGCCCGTTCCCTCTTCTACCATTACCAAT
  TCTGCAGTACAGTCTACCTCGAACGCATCTACGATGTCAGTTTCTAGTAAAGTAACAAAACCGATCCCCC
  GCAGTGAATCCTGCTCCCAGCCCGTGATGAATGGCAAATCCAAGCTGAACTCCAGCGTGCTGGTGCCCTA
  TGGCGCCGAGTCCTCTGAGGACTCTGACGAGGAGTCAAAGGGGCTGGGCAAGGAGAATGGGATTGGTACG
  ATTGTGAGCTCCCACTCTCCCGGCCAAGATGCCGAAGATGAGGAGGCCACTCCGCACGAGCTTCAAGAAC
  CCATGACCCTAAACGGTGCTAATAGTGCAGACAGCGACAGTGACCCGAAAGAAAACGGCCTAGCGCCTGA
  TGGTGCCAGCTGCCAAGGCCAGCCTGCCCTGCACTCAGAAAATCCCTTTGCTAAGGCAAACGGTCTTCCT
  GGAAAGTTGATGCCTGCTCCTTTGCTGTCTCTCCCAGAAGACAAAATCTTAGAGACCTTCAGGCTTAGCA
  ACAAACTGAAAGGCTCGACGGATGAAATGAGTGCACCTGGAGCAGAGAGGGGCCCTCCCGAGGACCGCGA
  CGCCGAGCCTCAGCCTGGCAGCCCCGCCGCCGAATCCCTGGAGGAGCCAGATGCGGCCGCCGGCCTCAGC
  AGCACCAAGAAGGCTCCGCCGCCCCGCGATCCCGGCACCCCCGCTACCAAAGAAGGCGCCTGGGAGGCCA
  TGGCCGTCGCCCCCGAGGAGCCTCCGCCCAGCGCCGGCGAGGACATCGTGGGGGACACAGCACCCCCTGA
  CCTGTGTGATCCCGGGAGCTTAACAGGCGATGCGAGCCCGTTGTCCCAGGACGCAAAGGGGATGATCGCG
  GAGGGCCCGCGGGACTCGGCGTTGGCGGAAGCCCCGGAAGGGTTGAGTCCGGCTCCGCCTGCGCGGTCGG
  AGGAGCCCTGCGAGCAGCCACTCCTTGTTCACCCCAGCGGGGACCACGCCCGGGACGCTCAGGACCCATC
  CCAGAGCTTGGGCGCACCCGAGGCCGCAGAGCGGCCGCCAGCTCCTGTGCTGGACATGGCCCCGGCCGGT
  CACCCGGAAGGGGACGCTGAGCCTAGCCCCGGCGAGAGGGTCGAGGACGCCGCGGCGCCGAAAGCCCCAG
  GCCCTTCCCCAGCGAAGGAGAAAATCGGCAGCCTCAGAAAGGTGGACCGAGGCCACTACCGCAGCCGGAG
  AGAGCGCTCGTCCAGCGGGGAGCCCGCCAGAGAGAGCAGGAGCAAGACTGAGGGCCACCGTCACCGGCGG
  CGCCGCACCTGCCCCCGGGAGCGCGACCGCCAGGACCGCCACGCCCCGGAGCACCACCCCGGCCACGGCG
  ACAGGCTCAGCCCTGGCGAGCGCCGCTCTCTGGGCAGGTGCAGTCACCACCACTCCCGACACCGGAGCGG
  GGTGGAGCTGGACTGGGTCAGACACCACTACACCGAGGGCGAGCGTGGCTGGGGCCGGGAGAAGTTCTAC
  CCCGACAGGCCGCGCTGGGACAGGTGCCGGTACTACCATGACAGGTACGCCCTGTACGCTGCCCGGGACT
  GGAAGCCCTTCCACGGCGGCCGCGAGCACGAGCGGGCCGGGCTGCACGAGCGGCCGCACAAGGACCACAA
  CCGGGGCCGTAGGGGCTGCGAGCCGGCCCGGGAGAGGGAGCGGCACCGCCCCAGCAGCCCCCGCGCAGGC
  GCGCCCCACGCCCTCGCCCCGCACCCCGACCGCTTCTCCCACGACAGAACTGCACTTGTAGCCGGAGACA
  ACTGTAACCTCTCTGATCGGTTTCACGAACACGAAAATGGAAAGTCCCGGAAACGGAGACACGACAGTGT
  GGAGAACAGTGACAGTCATGTTGAAAAGAAAGCCCGGAGGAGCGAACAGAAGGATCCTCTAGAAGAGCCT
  AAAGCAAAGAAGCACAAAAAATCAAAGAAGAAAAAGAAATCCAAAGACAAACACCGAGACCGCGACTCCA
  GGCATCAGCAGGACTCAGACCTCTCAGCAGCGTGCTCTGACGCTGACCTCCACAGACACAAAAAAAAGAA
  GAAGAAAAAGAAGAGACATTCAAGAAAATCAGAGGACTTTGTTAAAGATTCAGAACTGCACTTACCCAGG
  GTCACCAGCTTGGAGACTGTCGCCCAGTTCCGGAGAGCCCAGGGTGGCTTTCCTCTCTCTGGTGGCCCGC
  CTCTGGAAGGCGTCGGACCTTTCCGTGAGAAAACGAAACACTTACGGATGGAAAGCAGGGATGACAGGTG
  TCGTCTCTTTGAGTATGGCCAGGGTGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217405 representing NM_032172
  Red=Cloning site Green=Tags(s)
  
  MTIVDKASESSDPSAYQNQPGSSEAVSPGDMDAGSASWGAVSSLNDVSNHTLSLGPVPGAVVYSSSSVPD
  KSKPSPQKDQALGDGIAPPQKVLFPSEKICLKWQQTHRVGAGLQNLGNTCFANAALQCLTYTPPLANYML
  SHEHSKTCHAEGFCMMCTMQAHITQALSNPGDVIKPMFVINEMRRIARHFRFGNQEDAHEFLQYTVDAMQ
  KACLNGSNKLDRHTQATTLVCQIFGGYLRSRVKCLNCKGVSDTFDPYLDITLEIKAAQSVNKALEQFVKP
  EQLDGENSYKCSKCKKMVPASKRFTIHRSSNVLTLSLKRFANFTGGKIAKDVKYPEYLDIRPYMSQPNGE
  PIVYVLYAVLVHTGFNCHAGHYFCYIKASNGLWYQMNDSIVSTSDIRSVLSQQAYVLFYIRSHDVKNGGE
  LTHPTHSPGQSSPRPVISQRVVTNKQAAPGFIGPQLPSHMIKNPPHLNGTGPLKDTPSSSMSSPNGNSSV
  NRASPVNASASVQNWSVNRSSVIPEHPKKQKITISIHNKLPVRQCQSQPNLHSNSLENPTKPVPSSTITN
  SAVQSTSNASTMSVSSKVTKPIPRSESCSQPVMNGKSKLNSSVLVPYGAESSEDSDEESKGLGKENGIGT
  IVSSHSPGQDAEDEEATPHELQEPMTLNGANSADSDSDPKENGLAPDGASCQGQPALHSENPFAKANGLP
  GKLMPAPLLSLPEDKILETFRLSNKLKGSTDEMSAPGAERGPPEDRDAEPQPGSPAAESLEEPDAAAGLS
  STKKAPPPRDPGTPATKEGAWEAMAVAPEEPPPSAGEDIVGDTAPPDLCDPGSLTGDASPLSQDAKGMIA
  EGPRDSALAEAPEGLSPAPPARSEEPCEQPLLVHPSGDHARDAQDPSQSLGAPEAAERPPAPVLDMAPAG
  HPEGDAEPSPGERVEDAAAPKAPGPSPAKEKIGSLRKVDRGHYRSRRERSSSGEPARESRSKTEGHRHRR
  RRTCPRERDRQDRHAPEHHPGHGDRLSPGERRSLGRCSHHHSRHRSGVELDWVRHHYTEGERGWGREKFY
  PDRPRWDRCRYYHDRYALYAARDWKPFHGGREHERAGLHERPHKDHNRGRRGCEPARERERHRPSSPRAG
  APHALAPHPDRFSHDRTALVAGDNCNLSDRFHEHENGKSRKRRHDSVENSDSHVEKKARRSEQKDPLEEP
  KAKKHKKSKKKKKSKDKHRDRDSRHQQDSDLSAACSDADLHRHKKKKKKKKRHSRKSEDFVKDSELHLPR
  VTSLETVAQFRRAQGGFPLSGGPPLEGVGPFREKTKHLRMESRDDRCRLFEYGQGD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032172
• ORF Size: 3948 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032172.3
• RefSeq Size: 5109 bp
• RefSeq ORF: 3951 bp
• Locus ID: 84132
• UniProt ID: Q9H9J4
• Cytogenetics: 7p22.1
• Protein Families: Druggable Genome, Protease
• MW: 144.2 kDa
• Gene Summary: Deubiquitinating enzyme which may play an important role during spermatogenesis.[UniProtKB/Swiss-Prot Function]