CCBL2 (KYAT3) (NM_001008662) Human Tagged ORF Clone

CAT#: RC217351

CCBL2 (Myc-DDK-tagged)-Human cysteine conjugate-beta lyase 2 (CCBL2), transcript variant 2

Product Images

Circular map for RC217351

• 

  Western blot validation of overexpression lysate (Cat# LY423345) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217351 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified KYAT3 protein (Cat# TP317351). The protein was produced from HEK293T cells transfected with KYAT3 cDNA clone (Cat# RC217351) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCBL2
• Synonyms: CCBL2; KAT3; KATIII
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217351 representing NM_001008662
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACTGAAATTCACAAATGCAAAACGGATTGAAGGACTTGATAGTAATGTGTGGATTGAATTTACCA
  AATTGGCTGCAGACCCTTCTGTTGTGAATCTTGGCCAAGGCTTTCCAGATATATCCCCTCCTACATATGT
  AAAAGAAGAATTATCAAAGATTGCAGCAATCGATAGCCTGAATCAGTATACACGAGGCTTTGGCCATCCA
  TCACTTGTGAAAGCTCTGTCCTATCTGTATGAAAAGCTTTATCAAAAGCAAATTGATTCAAATAAAGAAA
  TCCTTGTGACAGTAGGAGCATATGGATCTCTTTTTAACACCATTCAAGCATTAATTGATGAGGGAGATGA
  AGTCATACTAATAGTGCCTTTCTATGACTGCTATGAGCCCATGGTGAGAATGGCTGGAGCAACACCTGTT
  TTTATTCCCCTGAGATCTAAACCTGTTTATGGAAAAAGATGGTCTAGTTCTGACTGGACATTAGATCCTC
  AAGAACTGGAAAGTAAATTTAATTCCAAAACCAAAGCTATTATACTAAATACTCCACATAACCCACTTGG
  CAAGGTGTATAACAGAGAGGAACTGCAAGTAATTGCTGACCTTTGCATCAAATATGACACACTCTGCATC
  AGCGATGAGGTTTATGAATGGCTTGTATATTCTGGAAATAAGCACTTAAAAATAGCTACTTTTCCAGGTA
  TGTGGGAGAGAACAATAACAATAGGAAGTGCTGGAAAGACTTTCAGTGTAACTGGCTGGAAGCTTGGCTG
  GTCCATTGGTCCAAATCATTTGATAAAACATTTACAGACAGTTCAACAAAACACGATTTATACTTGTGCA
  ACTCCTTTACAGGAAGCCTTGGCTCAAGCTTTCTGGATTGACATCAAGCGCATGGATGACCCAGAATGTT
  ACTTTAATTCTTTGCCAAAAGAGTTAGAAGTAAAAAGAGATCGGATGGTACGTTTACTTGAAAGTGTTGG
  CCTAAAACCCATAGTTCCTGATGGAGGATACTTCATCATCGCTGATGTGTCTTTGCTAGATCCAGACCTC
  TCTGATATGAAGAATAATGAGCCTTATGACTATAAGTTTGTGAAATGGATGACTAAACATAAGAAACTAT
  CAGCCATCCCCGTTTCAGCATTCTGTAACTCAGAGACTAAATCACAGTTTGAGAAGTTTGTGCGTTTTTG
  CTTCATTAAAAAAGACAGCACACTGGATGCTGCTGAAGAAATCATCAAGGCATGGAGTGTACAGAAGTCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217351 representing NM_001008662
  Red=Cloning site Green=Tags(s)
  
  MSLKFTNAKRIEGLDSNVWIEFTKLAADPSVVNLGQGFPDISPPTYVKEELSKIAAIDSLNQYTRGFGHP
  SLVKALSYLYEKLYQKQIDSNKEILVTVGAYGSLFNTIQALIDEGDEVILIVPFYDCYEPMVRMAGATPV
  FIPLRSKPVYGKRWSSSDWTLDPQELESKFNSKTKAIILNTPHNPLGKVYNREELQVIADLCIKYDTLCI
  SDEVYEWLVYSGNKHLKIATFPGMWERTITIGSAGKTFSVTGWKLGWSIGPNHLIKHLQTVQQNTIYTCA
  TPLQEALAQAFWIDIKRMDDPECYFNSLPKELEVKRDRMVRLLESVGLKPIVPDGGYFIIADVSLLDPDL
  SDMKNNEPYDYKFVKWMTKHKKLSAIPVSAFCNSETKSQFEKFVRFCFIKKDSTLDAAEEIIKAWSVQKS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008662
• ORF Size: 1260 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001008662.2, NP_001008662.1
• RefSeq Size: 1938 bp
• RefSeq ORF: 1263 bp
• Locus ID: 56267
• UniProt ID: Q6YP21
• Cytogenetics: 1p22.2
• MW: 47.6 kDa
• Gene Summary: This gene encodes an aminotransferase that transaminates kynurenine to form kynurenic acid, which is a metabolite of tryptophan. Multiple alternatively spliced transcript variants that encode different proteins have been described for this gene. This gene shares 5' exon structure with the RNA binding motif protein, X-linked-like 1 locus on chromosome 1, but the coding sequences are non-overlapping. [provided by RefSeq, Mar 2017]