DTX4 (NM_015177) Human Tagged ORF Clone

CAT#: RC217321

DTX4 (Myc-DDK-tagged)-Human deltex homolog 4 (Drosophila) (DTX4)

Product Images

Circular map for RC217321

• 

  Western blot validation of overexpression lysate (Cat# LY414733) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217321 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DTX4
• Synonyms: RNF155
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217321 representing NM_015177
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCTGGCCTCGGCCGTGGTGGTCTGGGAATGGCTGAACGAGCACGGCCGCTGGCGTCCCTACAGCC
  CAGCGGTGAGCCACCACATCGAGGCGGTGGTCCGCGCCGGCCCCCGCGCGGGGGGCAGCGTGGTGCTGGG
  CCAGGTGGACAGCCGTCTCGCGCCCTACATCATCGACCTGCAGTCCATGAACCAGTTCCGCCAAGACACG
  GGAACTCTCCGCCCAGTTCGCCGCAACTACTACGACCCCTCCTCGGCCCCTGGGAAGGGCGTGGTGTGGG
  AGTGGGAGAACGACAATGGCTCCTGGACGCCCTACGACATGGAAGTGGGCATCACCATCCAGCATGCCTA
  TGAGAAGCAGCACCCCTGGATCGACCTCACTTCCATTGGCTTTAGCTACGTAATTGACTTCAACACCATG
  GGCCAGATCAACCGTCAGACCCAGCGCCAACGCCGCGTCCGCCGGCGCCTCGACCTCATCTACCCCATGG
  TCACAGGGACCTTGCCTAAGGCTCAGTCCTGGCCAGTCAGCCCTGGGCCAGCCACCTCGCCCCCCATGTC
  CCCCTGCTCCTGTCCCCAGTGTGTCTTGGTGATGAGTGTTAAGGCAGCCGTGGTCAATGGCAGCACTGGG
  CCCCTACAGCTGCCAGTGACCCGCAAGAACATGCCGCCTCCTGGAGTGGTCAAGCTACCCCCACTGCCAG
  GCTCTGGGGCCAAGCCACTGGACAGCACAGGCACCATTCGAGGCCCACTGAAGACCGCCCCATCGCAGGT
  GATCCGGAGACAAGCCTCCAGCATGCCCACTGGGACAACCATGGGCTCTCCTGCCAGTCCCCCAGGACCC
  AACAGCAAGACCGGAAGGGTGGCCCTGGCCACCTTGAATCGTACCAACCTGCAGCGACTGGCCATTGCCC
  AGTCCCGGGTGCTGATCGCCTCTGGGGTCCCCACAGTCCCAGTGAAGAACCTAAATGGGTCCAGTCCTGT
  CAACCCTGCCTTGGCAGGAATCACTGGGATCCTCATGAGTGCAGCGGGGCTGCCTGTGTGTCTCACCAGG
  CCACCAAAGCTGGTCCTACACCCACCCCCCGTCAGCAAGAGTGAAATAAAATCCATCCCAGGGGTTTCCA
  ACACAAGCCGCAAGACCACCAAAAAACAAGCCAAGAAAGGTAAAACCCCAGAGGAAGTGCTAAAAAAATA
  TCTACAGAAAGTCCGGCACCCACCAGATGAGGACTGCACCATCTGTATGGAACGCCTCACGGCCCCCTCA
  GGCTACAAGGGCCCGCAGCCTACGGTAAAACCTGACCTGGTAGGGAAGCTGTCCAGATGCGGCCACGTCT
  ACCACATCTACTGCTTGGTTGCCATGTACAACAATGGGAACAAGGATGGAAGTTTGCAGTGTCCAACCTG
  CAAGACCATTTATGGGGTGAAGACAGGCACCCAACCTCCAGGGAAGATGGAGTACCACCTCATCCCCCAC
  TCCTTGCCTGGCCACCCAGACTGCAAAACCATCCGGATCATCTACAGCATCCCCCCCGGCATTCAGGGAC
  CGGAACACCCGAATCCTGGGAAGAGTTTCAGCGCCCGAGGCTTCCCACGACACTGTTACCTTCCGGACAG
  CGAGAAAGGGAGAAAAGTTCTGAAGCTGCTGCTCGTGGCCTGGGATCGCCGCCTCATTTTTGCCATTGGC
  ACCTCCAGCACCACAGGCGAGTCAGACACCGTCATCTGGAATGAGGTCCACCACAAGACAGAGTTTGGCT
  CTAATCTCACTGGCCATGGCTACCCAGATGCCAATTACCTGGATAATGTGCTGGCTGAACTGGCTGCCCA
  GGGCATCTCTGAGGACAGCACTGCCCAGGAGAAGGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217321 representing NM_015177
  Red=Cloning site Green=Tags(s)
  
  MLLASAVVVWEWLNEHGRWRPYSPAVSHHIEAVVRAGPRAGGSVVLGQVDSRLAPYIIDLQSMNQFRQDT
  GTLRPVRRNYYDPSSAPGKGVVWEWENDNGSWTPYDMEVGITIQHAYEKQHPWIDLTSIGFSYVIDFNTM
  GQINRQTQRQRRVRRRLDLIYPMVTGTLPKAQSWPVSPGPATSPPMSPCSCPQCVLVMSVKAAVVNGSTG
  PLQLPVTRKNMPPPGVVKLPPLPGSGAKPLDSTGTIRGPLKTAPSQVIRRQASSMPTGTTMGSPASPPGP
  NSKTGRVALATLNRTNLQRLAIAQSRVLIASGVPTVPVKNLNGSSPVNPALAGITGILMSAAGLPVCLTR
  PPKLVLHPPPVSKSEIKSIPGVSNTSRKTTKKQAKKGKTPEEVLKKYLQKVRHPPDEDCTICMERLTAPS
  GYKGPQPTVKPDLVGKLSRCGHVYHIYCLVAMYNNGNKDGSLQCPTCKTIYGVKTGTQPPGKMEYHLIPH
  SLPGHPDCKTIRIIYSIPPGIQGPEHPNPGKSFSARGFPRHCYLPDSEKGRKVLKLLLVAWDRRLIFAIG
  TSSTTGESDTVIWNEVHHKTEFGSNLTGHGYPDANYLDNVLAELAAQGISEDSTAQEKD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015177
• ORF Size: 1857 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015177.2
• RefSeq Size: 5795 bp
• RefSeq ORF: 1860 bp
• Locus ID: 23220
• UniProt ID: Q9Y2E6
• Cytogenetics: 11q12.1
• Protein Families: Druggable Genome
• Protein Pathways: Notch signaling pathway
• MW: 67.1 kDa
• Gene Summary: Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations (By similarity). Functions as a ubiquitin ligase protein in vivo, mediating 'Lys48'-linked polyubiquitination and promoting degradation of TBK1, targeting to TBK1 requires interaction with NLRP4.[UniProtKB/Swiss-Prot Function]