SLC26A1 (NM_213613) Human Tagged ORF Clone

CAT#: RC217255

SLC26A1 (Myc-DDK-tagged)-Human solute carrier family 26 (sulfate transporter), member 1 (SLC26A1), transcript variant 3

Product Images

Circular map for RC217255

• 

  Western blot validation of overexpression lysate (Cat# LY403889) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC217255 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC26A1
• Synonyms: CAON; EDM4; SAT-1; SAT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC217255 representing NM_213613
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGAGTCCCCTGAGCCTCTGCAGCAGGGCAGAGGGCCGGTGCCGGTCCGACGGCAGCGCCCAGCAC
  CCCGGGGTCTGCGTGAGATGCTGAAGGCCAGGCTGTGGTGCAGCTGCTCGTGCAGTGTGCTGTGCGTCCG
  GGCGCTGGTGCAGGACCTGCTCCCCGCCACGCGCTGGCTGCGTCAGTACCGCCCGCGGGAGTACCTGGCA
  GGCGACGTCATGTCTGGGCTGGTCATCGGCATCATCCTGGTGCCGCAGGCCATCGCCTACTCATTGCTGG
  CCGGGCTGCAGCCCATCTACAGCCTCTATACGTCCTTCTTCGCCAACCTCATCTACTTCCTCATGGGCAC
  CTCACGGCATGTCTCCGTGGGCATCTTCAGCCTGCTTTGCCTCATGGTGGGGCAGGTGGTGGACCGGGAG
  CTCCAGCTGGCCGGCTTTGACCCCTCCCAGGACGGCCTGCAGCCCGGAGCCAACAGCAGCACCCTCAACG
  GCTCGGCTGCCATGCTGGACTGCGGGCGTGACTGCTACGCCATCCGTGTCGCCACCGCCCTCACGCTGAT
  GACCGGGCTTTACCAGGTCCTCATGGGCGTCCTCCGGCTGGGCTTCGTGTCCGCCTACCTCTCACAGCCA
  CTGCTCGATGGCTTTGCCATGGGGGCCTCCGTGACCATCCTGACCTCGCAGCTCAAACACCTGCTGGGCG
  TGCGGATCCCGCGGCACCAGGGGCCCGGCATGGTGGTCCTCACATGGCTGAGCCTGCTGCGCGGCGCCGG
  GCAGGCCAACGTGTGCGACGTGGTCACCAGCACGGTGTGCCTGGCGGTGCTGCTAGCCGCGAAGGAGCTC
  TCAGACCGCTACCGACACCGCCTGAGGGTGCCGCTGCCCACGGAGCTGCTGGTCATCGTGGTGGCCACAC
  TCGTGTCGCACTTCGGGCAGCTCCACAAGCGCTTTGGCTCGAGCGTGGCTGGCGACATCCCCACGGGTTT
  CATGCCCCCTCAGGTCCCAGAGCCCAGGCTGATGCAGCGTGTGGCTTTGGATGCCGTGGCCCTGGCCCTC
  GTGGCTGCCGCCTTCTCCATCTCGCTGGCGGAGATGTTCGCCCGCAGTCACGGCTACTCTGTGCGTGCCA
  ACCAGGAGCTGCTGGCTGTGGGCTGCTGCAACGTGCTACCCGCCTTCCTCCACTGCTTCGCCACCAGCGC
  CGCCCTGGCCAAGAGCCTGGTGAAGACAGCCACTGGCTGCCGGACACAGCTGTCCAGCGTGGTCAGCGCC
  ACCGTGGTGCTGCTGGTGCTGCTGGCGCTGGCACCGCTGTTCCACGACCTACAGCGAAGCGTGCTGGCCT
  GCGTCATCGTGGTCAGCCTGCGGGGGGCCCTGCGCAAGGTGTGGGACCTCCCGCGGCTGTGGCGGATGAG
  CCCGGCTGACGCGCTGGTCTGGGCAGGCACCGCGGCCACCTGTATGCTGGTCAGCACAGAGGCCGGGCTG
  CTGGCTGGCGTCATCCTCTCGCTGCTCAGCCTGGCCGGCCGCACCCAACGCCCACGCACCGCCCTGCTGG
  CCCGCATCGGGGACACGGCCTTCTACGAGGATGCCACAGAGTTCGAGGGCCTCGTCCCTGAGCCCGGCGT
  GCGGGTGTTCCGCTTTGGGGGGCCGCTGTACTATGCCAACAAGGACTTCTTCCTGCAGTCACTCTACAGC
  CTCACGGGGCTGGACGCAGGGTGCATGGCTGCCAGGAGGAAGGAGGGGGGCTCAGAGACGGGGGTCGGTG
  AGGGAGGCCCTGCCCAGGGCGAGGACCTGGGCCCGGTTAGCACCAGGGCTGCGCTGGTGCCCGCAGCGGC
  CGGCTTCCACACAGTGGTCATCGACTGCGCCCCGCTGCTGTTCCTAGACGCAGCCGGTGTGAGCACGCTG
  CAGGACCTGCGCCGAGACTACGGGGCCCTGGGCATCAGCCTGCTGCTAGCCTGCTGCAGCCCGCCTGTGA
  GAGACATTCTGAGCAGAGGAGGCTTCCTCGGGGAGGGCCCCGGGGACACGGCTGAGGAGGAGCAGCTGTT
  CCTCAGTGTGCACGATGCCGTGCAGACAGCACGAGCCCGCCACAGGGAGCTGGAGGCCACCGATGCCCAT
  CTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC217255 representing NM_213613
  Red=Cloning site Green=Tags(s)
  
  MDESPEPLQQGRGPVPVRRQRPAPRGLREMLKARLWCSCSCSVLCVRALVQDLLPATRWLRQYRPREYLA
  GDVMSGLVIGIILVPQAIAYSLLAGLQPIYSLYTSFFANLIYFLMGTSRHVSVGIFSLLCLMVGQVVDRE
  LQLAGFDPSQDGLQPGANSSTLNGSAAMLDCGRDCYAIRVATALTLMTGLYQVLMGVLRLGFVSAYLSQP
  LLDGFAMGASVTILTSQLKHLLGVRIPRHQGPGMVVLTWLSLLRGAGQANVCDVVTSTVCLAVLLAAKEL
  SDRYRHRLRVPLPTELLVIVVATLVSHFGQLHKRFGSSVAGDIPTGFMPPQVPEPRLMQRVALDAVALAL
  VAAAFSISLAEMFARSHGYSVRANQELLAVGCCNVLPAFLHCFATSAALAKSLVKTATGCRTQLSSVVSA
  TVVLLVLLALAPLFHDLQRSVLACVIVVSLRGALRKVWDLPRLWRMSPADALVWAGTAATCMLVSTEAGL
  LAGVILSLLSLAGRTQRPRTALLARIGDTAFYEDATEFEGLVPEPGVRVFRFGGPLYYANKDFFLQSLYS
  LTGLDAGCMAARRKEGGSETGVGEGGPAQGEDLGPVSTRAALVPAAAGFHTVVIDCAPLLFLDAAGVSTL
  QDLRRDYGALGISLLLACCSPPVRDILSRGGFLGEGPGDTAEEEQLFLSVHDAVQTARARHRELEATDAH
  L
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_213613
• ORF Size: 2103 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_213613.4
• RefSeq Size: 3673 bp
• RefSeq ORF: 2106 bp
• Locus ID: 10861
• UniProt ID: Q9H2B4
• Cytogenetics: 4p16.3
• Protein Families: Transmembrane
• MW: 74.8 kDa
• Gene Summary: This gene is a member of a family of sulfate/anion transporter genes. Family members are well conserved in their genomic (number and size of exons) and protein (aa length among species) structures, but have markedly different tissue expression patterns. This gene is primarily expressed in the liver, pancreas, and brain. Three splice variants that encode different isoforms have been identified. [provided by RefSeq, Jul 2008]