SCD5 (NM_001037582) Human Tagged ORF Clone

CAT#: RC216884

SCD5 (Myc-DDK-tagged)-Human stearoyl-CoA desaturase 5 (SCD5), transcript variant 1

Product Images

Circular map for RC216884

• 

  Western blot validation of overexpression lysate (Cat# LY400413) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216884 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SCD5
• Synonyms: ACOD4; FADS4; HSCD5; SCD2; SCD4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216884 representing NM_001037582
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGGCCCGGCCACCGACGCGGGGAAGATCCCTTTCTGCGACGCCAAGGAAGAAATCCGTGCCGGGC
  TCGAAAGCTCTGAGGGCGGCGGCGGCCCGGAGAGGCCAGGCGCGCGCGGGCAGCGGCAGAACATCGTCTG
  GAGGAATGTCGTCCTGATGAGCTTGCTCCACTTGGGGGCCGTGTACTCCCTGGTGCTCATCCCCAAAGCC
  AAGCCACTCACTCTGCTCTGGGCCTACTTCTGCTTCCTCCTGGCCGCTCTGGGTGTGACAGCTGGTGCCC
  ATCGCTTGTGGAGCCACAGGTCCTACCGGGCCAAGCTGCCTCTGAGGATATTTCTGGCTGTCGCCAACTC
  CATGGCTTTCCAGAATGACATCTTCGAGTGGTCCAGGGACCACCGAGCCCACCACAAGTACTCAGAGACG
  GATGCTGACCCCCACAATGCCCGCCGGGGCTTCTTCTTCTCCCATATTGGGTGGCTGTTTGTTCGCAAGC
  ATCGAGATGTTATTGAGAAGGGGAGAAAGCTTGACGTCACTGACCTGCTTGCTGATCCTGTGGTCCGGAT
  CCAGAGAAAGTACTATAAGATCTCCGTGGTGCTCATGTGCTTTGTGGTCCCCACGCTGGTGCCCTGGTAC
  ATCTGGGGAGAGAGTCTGTGGAATTCCTACTTCTTGGCCTCTATTCTCCGCTATACCATCTCACTCAACA
  TCAGCTGGCTGGTCAACAGCGCCGCCCACATGTATGGAAACCGGCCCTATGACAAGCACATCAGCCCTCG
  GCAGAACCCACTCGTCGCTCTGGGTGCCATTGGTGAAGGCTTCCATAATTACCATCACACCTTTCCCTTT
  GACTACTCTGCGAGTGAATTTGGCTTAAATTTTAACCCAACCACCTGGTTCATTGATTTCATGTGCTGGC
  TGGGGCTGGCCACTGACCGCAAACGGGCAACCAAGCCGATGATCGAGGCCCGGAAGGCCAGGACTGGAGA
  CAGCAGTGCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216884 representing NM_001037582
  Red=Cloning site Green=Tags(s)
  
  MPGPATDAGKIPFCDAKEEIRAGLESSEGGGGPERPGARGQRQNIVWRNVVLMSLLHLGAVYSLVLIPKA
  KPLTLLWAYFCFLLAALGVTAGAHRLWSHRSYRAKLPLRIFLAVANSMAFQNDIFEWSRDHRAHHKYSET
  DADPHNARRGFFFSHIGWLFVRKHRDVIEKGRKLDVTDLLADPVVRIQRKYYKISVVLMCFVVPTLVPWY
  IWGESLWNSYFLASILRYTISLNISWLVNSAAHMYGNRPYDKHISPRQNPLVALGAIGEGFHNYHHTFPF
  DYSASEFGLNFNPTTWFIDFMCWLGLATDRKRATKPMIEARKARTGDSSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037582
• ORF Size: 990 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037582.3
• RefSeq Size: 3030 bp
• RefSeq ORF: 993 bp
• Locus ID: 79966
• UniProt ID: Q86SK9
• Cytogenetics: 4q21.22
• Protein Families: Transmembrane
• Protein Pathways: Biosynthesis of unsaturated fatty acids, PPAR signaling pathway
• MW: 37.4 kDa
• Gene Summary: Stearoyl-CoA desaturase (SCD; EC 1.14.99.5) is an integral membrane protein of the endoplasmic reticulum that catalyzes the formation of monounsaturated fatty acids from saturated fatty acids. SCD may be a key regulator of energy metabolism with a role in obesity and dislipidemia. Four SCD isoforms, Scd1 through Scd4, have been identified in mouse. In contrast, only 2 SCD isoforms, SCD1 (MIM 604031) and SCD5, have been identified in human. SCD1 shares about 85% amino acid identity with all 4 mouse SCD isoforms, as well as with rat Scd1 and Scd2. In contrast, SCD5 shares limited homology with the rodent SCDs and appears to be unique to primates (Wang et al., 2005 [PubMed 15907797]).[supplied by OMIM, Mar 2008]