INTS8 (NM_017864) Human Tagged ORF Clone

CAT#: RC216855

INTS8 (Myc-DDK-tagged)-Human integrator complex subunit 8 (INTS8)

Product Images

Circular map for RC216855

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: INTS8
• Synonyms: C8orf52; INT8; NEDCHS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216855 representing NM_017864
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGCGGAGGCGGCGGACCGGGAGGCGGCCACCTCCAGCCGGCCCTGCACCCCGCCGCAGACCTGCT
  GGTTTGAGTTTCTGCTGGAGGAGTCACTGTTGGAGAAACATCTGCGCAAGCCCTGCCCGGATCCTGCACC
  AGTTCAACTTATAGTTCAGTTTTTGGAACAGGCTTCCAAACCTTCAGTTAATGAACAAAACCAAGTTCAA
  CCTCCGCCTGATAACAAGAGAAATCGTATTTTAAAACTACTTGCTCTTAAAGTTGCTGCACATTTGAAGT
  GGGACTTAGACATATTAGAGAAAAGTTTGTCTGTTCCAGTATTGAATATGCTACTAAATGAACTACTCTG
  CATCAGTAAAGTTCCTCCTGGGACAAAGCATGTAGACATGGATCTGGCCACTTTACCTCCGACCACTGCC
  ATGGCTGTACTTCTCTATAATAGATGGGCAATTAGGACAATTGTTCAAAGTAGTTTTCCAGTCAAACAGG
  CAAAACCCGGACCCCCTCAGTTAAGTGTTATGAATCAAATGCAACAGGAAAAAGAGCTAACAGAAAACAT
  TTTGAAAGTGCTCAAAGAACAAGCTGCTGATTCTATTTTGGTACTAGAAGCAGCCCTAAAATTAAACAAG
  GATCTTTATGTCCATACGATGAGAACTCTAGATTTATTGGCCATGGAACCAGGCATGGTAAATGGAGAAA
  CTGAGAGTTCTACTGCTGGATTGAAAGTCAAAACCGAAGAAATGCAGTGCCAGGTGTGCTATGATTTGGG
  CGCAGCATACTTCCAGCAAGGCTCCACAAATTCAGCTGTCTATGAAAATGCCAGGGAAAAATTTTTTAGA
  ACCAAAGAACTAATTGCAGAGATAGGTTCATTATCTCTTCATTGTACCATAGATGAGAAGCGGTTAGCTG
  GCTATTGTCAAGCATGTGATGTTCTTGTACCTTCTTCTGATAGTACATCTCAACAGTTGACTCCATATAG
  TCAAGTCCATATTTGTTTGAGATCTGGCAACTATCAGGAGGTAATACAGATTTTCATTGAAGACAACTTA
  ACCTTGAGTTTACCTGTCCAGTTCCGACAGTCAGTCCTAAGAGAACTCTTTAAGAAAGCTCAACAGGGAA
  ATGAAGCTCTAGATGAAATCTGTTTTAAAGTTTGTGCCTGTAATACAGTCCGTGATATACTGGAAGGCAG
  AACAATTAGTGTTCAATTTAACCAGCTATTTCTTAGACCAAATAAAGAGAAAATAGACTTTCTTCTTGAG
  GTATGTTCAAGATCAGTAAATTTAGAAAAAGCTTCAGAGTCTTTGAAAGGAAACATGGCTGCTTTTCTAA
  AGAATGTGTGTCTGGGGTTGGAAGATCTGCAGTATGTTTTCATGATTTCTTCACATGAGCTTTTCATTAC
  ATTGTTGAAAGATGAAGAACGAAAGCTACTTGTTGATCAGATGAGGAAGAGATCCCCTAGAGTAAATCTG
  TGCATTAAACCTGTAACTTCATTTTATGATATCCCAGCTTCAGCAAGTGTCAACATTGGTCAGTTAGAGC
  ATCAACTTATATTGTCAGTGGATCCTTGGAGGATTAGACAAATTTTAATTGAATTACATGGTATGACTTC
  AGAGCGCCAGTTCTGGACAGTGTCTAATAAGTGGGAAGTACCTTCTGTCTATAGTGGTGTTATCCTGGGA
  ATTAAAGACAATTTAACAAGAGATTTGGTTTATATTCTTATGGCCAAAGGTTTGCACTGCAGTACTGTTA
  AGGACTTTTCCCATGCTAAACAGCTCTTTGCTGCTTGTTTGGAGTTGGTAACAGAGTTCTCACCGAAGCT
  TCGTCAGGTCATGCTGAATGAGATGTTGCTTTTGGATATTCATACACACGAAGCTGGGACAGGGCAGGCA
  GGAGAGAGACCGCCATCCGACCTTATAAGTAGAGTACGAGGCTATCTGGAAATGAGGCTTCCTGATATTC
  CTCTTCGTCAAGTTATAGCTGAGGAATGTGTTGCCTTTATGTTAAACTGGAGAGAAAATGAATACCTTAC
  ACTCCAAGTTCCTGCATTTTTGCTTCAGAGTAATCCATATGTAAAGCTTGGACAGCTTTTAGCAGCTACA
  TGCAAAGAACTTCCAGGCCCTAAAGAAAGTAGACGGACTGCCAAAGACCTTTGGGAAGTTGTTGTTCAAA
  TCTGTAGTGTGTCCAGTCAGCACAAACGAGGAAATGATGGCAGAGTTAGTTTAATAAAACAGAGGGAATC
  TACGTTAGGTATCATGTATCGGAGTGAACTGCTTTCTTTTATCAAAAAATTACGAGAACCACTCGTTTTG
  ACTATTATTTTATCACTCTTTGTGAAACTTCACAATGTTCGGGAGGACATTGTGAATGATATTACAGCTG
  AACACATTTCTATTTGGCCATCTTCCATTCCCAACCTCCAGTCTGTGGACTTTGAAGCTGTGGCAATCAC
  AGTGAAAGAGCTAGTTCGATATACACTCAGTATAAATCCAAATAACCATTCTTGGTTAATTATCCAGGCA
  GATATTTACTTTGCAACGAATCAGTATTCAGCAGCTCTTCACTATTACCTCCAGGCAGGAGCTGTGTGTT
  CTGACTTCTTTAACAAGGCTGTGCCCCCTGATGTTTATACAGACCAGGTAATAAAACGAATGATAAAATG
  TTGTTCTTTGCTGAATTGCCACACACAGGTGGCCATTTTATGTCAGTTCCTCAGAGAAATTGACTACAAA
  ACAGCGTTTAAATCTCTGCAAGAACAAAACAGTCATGATGCTATGGACTCCTACTACGACTACATATGGG
  ATGTTACCATTTTGGAATACTTGACTTATCTTCATCATAAAAGAGGAGAAACAGATAAAAGACAAATTGC
  AATCAAAGCCATCGGCCAGACAGAGTTGAATGCAAGCAATCCAGAAGAAGTGTTACAGCTGGCAGCGCAG
  AGAAGGAAAAAAAAGTTTCTCCAAGCAATGGCAAAACTTTACTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216855 representing NM_017864
  Red=Cloning site Green=Tags(s)
  
  MSAEAADREAATSSRPCTPPQTCWFEFLLEESLLEKHLRKPCPDPAPVQLIVQFLEQASKPSVNEQNQVQ
  PPPDNKRNRILKLLALKVAAHLKWDLDILEKSLSVPVLNMLLNELLCISKVPPGTKHVDMDLATLPPTTA
  MAVLLYNRWAIRTIVQSSFPVKQAKPGPPQLSVMNQMQQEKELTENILKVLKEQAADSILVLEAALKLNK
  DLYVHTMRTLDLLAMEPGMVNGETESSTAGLKVKTEEMQCQVCYDLGAAYFQQGSTNSAVYENAREKFFR
  TKELIAEIGSLSLHCTIDEKRLAGYCQACDVLVPSSDSTSQQLTPYSQVHICLRSGNYQEVIQIFIEDNL
  TLSLPVQFRQSVLRELFKKAQQGNEALDEICFKVCACNTVRDILEGRTISVQFNQLFLRPNKEKIDFLLE
  VCSRSVNLEKASESLKGNMAAFLKNVCLGLEDLQYVFMISSHELFITLLKDEERKLLVDQMRKRSPRVNL
  CIKPVTSFYDIPASASVNIGQLEHQLILSVDPWRIRQILIELHGMTSERQFWTVSNKWEVPSVYSGVILG
  IKDNLTRDLVYILMAKGLHCSTVKDFSHAKQLFAACLELVTEFSPKLRQVMLNEMLLLDIHTHEAGTGQA
  GERPPSDLISRVRGYLEMRLPDIPLRQVIAEECVAFMLNWRENEYLTLQVPAFLLQSNPYVKLGQLLAAT
  CKELPGPKESRRTAKDLWEVVVQICSVSSQHKRGNDGRVSLIKQRESTLGIMYRSELLSFIKKLREPLVL
  TIILSLFVKLHNVREDIVNDITAEHISIWPSSIPNLQSVDFEAVAITVKELVRYTLSINPNNHSWLIIQA
  DIYFATNQYSAALHYYLQAGAVCSDFFNKAVPPDVYTDQVIKRMIKCCSLLNCHTQVAILCQFLREIDYK
  TAFKSLQEQNSHDAMDSYYDYIWDVTILEYLTYLHHKRGETDKRQIAIKAIGQTELNASNPEEVLQLAAQ
  RRKKKFLQAMAKLYF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017864
• ORF Size: 2985 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017864.4
• RefSeq Size: 3393 bp
• RefSeq ORF: 2988 bp
• Locus ID: 55656
• UniProt ID: Q75QN2
• Cytogenetics: 8q22.1
• MW: 112.9 kDa
• Gene Summary: This gene encodes a subunit of the Integrator complex which is involved in the cleavage of small nuclear RNAs U1 and U2 within the nucleus. The encoded protein associates with RNA polymerase II and is recruited to the U1 and U2 small nuclear RNA genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012]