SH3RF3 (NM_001099289) Human Tagged ORF Clone

CAT#: RC216773

SH3RF3 (Myc-DDK-tagged)-Human SH3 domain containing ring finger 3 (SH3RF3)

Product Images

Circular map for RC216773

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  Western blot validation of overexpression lysate (Cat# LY420427) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216773 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified SH3RF3 protein (Cat# TP316773). The protein was produced from HEK293T cells transfected with SH3RF3 cDNA clone (Cat# RC216773) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SH3RF3
• Synonyms: POSH2; SH3MD4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216773 representing NM_001099289
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTCGGAGCGTCCTGGCTGTGCGCATCCAAGGCGGCCGCCGCTGCTGCGCAGAGCGAGGGCGACG
  AGGACAGGCCAGGCGAGCGACGGCGGCGTCGGGCGGCGGCCACCGCCGCGGGGGCGGGCGAGGACATGGA
  CGAGTCGTCGCTGCTGGACCTGCTGGAGTGCTCCGTGTGTCTGGAGCGCCTGGACACCACGGCCAAGGTG
  CTGCCATGCCAACACACTTTCTGCCGCCGCTGCCTGGAGAGCATCGTGTGCTCGCGCCACGAGCTGCGCT
  GCCCCGAGTGCCGCATCCTGGTGGGCTGCGGCGTGGACGAACTGCCCGCCAACATCTTGCTGGTGCGACT
  GCTGGACGGCATCCGTCAGCGGCCCCGCGCGGGCACCAGCCCCGGCGGCAGCCCGCCCGCGCGTCCCATC
  CCAGGCCAGAGTGCGGCCCCCACGCTCGCGGGCGGCGGGGGCGGCGCGGCAGGCAGCACCCCGGGTTCCC
  CGGTTTTCCTCTCCGCGGCCGCGGGCAGCACCGCCGGCAGTCTGCGGGAGCTGGCGACCAGCAGGACCGC
  GCCGGCGGCAAAGAATCCCTGCCTGCTTCCCTATGGCAAGGCCCTCTACAGCTACGAGGGGAAGGAACCT
  GGTGACCTCAAGTTCAACAAGGGGGACATCATCGTCCTGCGGCGCAAGGTGGATGAACAGTGGTACCACG
  GCGAGCTGCACGGCACACAGGGCTTCCTCCCAGCCAGCTATATCCAGTGCATCCAGCCCTTGCCACACGC
  CCCGCCCCAGGGAAAAGCACTTTATGATTTCGAGATGAAGGACAAAGACCAAGACAAGGACTGTCTGACC
  TTCACCAAGGACGAGATTCTGACGGTGCTCAGGAGAGTGGATGAGAACTGGGCGGAAGGCATGCTGGGAG
  ACAAGATCGGGATCTTCCCGCTCCTGTACGTGGAGCTCAATGACTCCGCCAAGCAGCTCATTGAGATGGA
  CAAGCCATGCCCAGCCGCTGCATCCAGCTGCAATGCCTCCCTGCCCTCTGACTCCGGCGCTGTGGCCAGC
  GTGGCCCCAAGTCCCACTTTAAGCAGCTCAGGGGCGGTCAGTGCCTTTCAGCGGCGTGTGGATGGCAAGA
  AGAACACCAAGAAACGCCACTCCTTCACCGCGCTCAGTGTGACGCACAGATCCTCCCAGGCTGCCAGCCA
  CAGGCATTCCATGGAAATTAGTGCTCCAGTGTTGATCAGCTCCAGCGATCCCCGAGCCGCGGCCAGGATT
  GGAGACCTTGCTCATCTGTCGTGCGCTGCTCCCACCCAGGATGTCTCCTCCTCGGCGGGATCTACCCCCA
  CGGCTGTCCCACGGGCTGCCTCGGTGTCTGGAGAGCAGGGCACGCCTCCCAAGGTCCAGCTGCCCCTCAA
  CGTGTACCTGGCGCTCTACGCCTACAAGCCCCAGAAGAGTGACGAGCTGGAGCTGCACAAGGGAGAGATG
  TACCGGGTCCTCGAGAAGTGTCAGGATGGCTGGTTCAAGGGGGCGTCTCTGAGGACCGGGGTCTCTGGGG
  TGTTCCCCGGAAACTACGTGACACCCGTTTCCAGGGTGCCTGCAGGAGGGGCAGGGCCGCCCCGGAATAA
  TGTAGTCGGAGGGTCTCCACTGGCCAAAGGGATAACCACAACCATGCACCCAGGCAGTGGGAGTCTGAGC
  AGCCTGGCCACTGCCACCAGGCCCGCCCTGCCCATCACCACTCCCCAGGCCCACGCCCAGCACCCCACAG
  CCTCGCCCCCAACAGGCAGCTGTCTACGGCACTCAGCCCAGCCAACGGCCAGCCAAGCCCGGAGCACCAT
  TTCAACAGCTGCCCACTCTGCAGCCCAGGCTCAGGACCGGCCAACTGCCACCGTGTCACCCCTGCGCACC
  CAGAACTCTCCATCCCGCCTGCCTGCCACCAGCCTCAGGCCCCACTCGGTGGTGTCCCCGCAGCACAGCC
  ACCAGCCCCCGGTGCAGATGTGCCCACGGCCGGCCATCCCCCTCACATCAGCAGCATCAGCCATCACACC
  TCCCAACGTCAGTGCCGCAAACCTCAACGGGGAGGCTGGAGGGGGGCCCATCGGTGTTCTGTCCACATCC
  AGCCCCACCAACACGGGATGCAAACTAGACGAGAAGAAAAGTGAAAAGAAAGAGAAGAAGAGTGGGCTCC
  TGAAGCTTCTAGCCGGAGCATCCACCAAGAAGAAGTCACGCTCCCCGCCATCTGTGTCTCCAACCCACGA
  CCCCCAGGTGGCCGTGGACGCCCTGCTCCAAGGTGCAGTGGGCCCCGAAGTGTCCTCACTGTCCATCCAC
  GGCAGGGCAGGGTCCTGCCCCATAGAGAGCGAGATGCAGGGTGCCATGGGGATGGAGCCTCTGCACAGGA
  AGGCAGGCTCCTTGGATCTAAACTTCACATCTCCTTCCCGGCAAGCTCCGCTGTCCATGGCTGCCATCCG
  CCCCGAGCCCAAGCTGTTGCCCAGAGAGAGGTACCGCGTGGTGGTCTCGTACCCACCCCAGAGTGAGGCG
  GAGATCGAGCTGAAGGAAGGCGACATCGTCTTTGTGCACAAGAAGCGTGAGGACGGCTGGTACAAGGGGA
  CCCTGCAGCGGAACGGCCGCACAGGCCTCTTCCCGGGCAGCTTCGTCGAGAGCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216773 representing NM_001099289
  Red=Cloning site Green=Tags(s)
  
  MLLGASWLCASKAAAAAAQSEGDEDRPGERRRRRAAATAAGAGEDMDESSLLDLLECSVCLERLDTTAKV
  LPCQHTFCRRCLESIVCSRHELRCPECRILVGCGVDELPANILLVRLLDGIRQRPRAGTSPGGSPPARPI
  PGQSAAPTLAGGGGGAAGSTPGSPVFLSAAAGSTAGSLRELATSRTAPAAKNPCLLPYGKALYSYEGKEP
  GDLKFNKGDIIVLRRKVDEQWYHGELHGTQGFLPASYIQCIQPLPHAPPQGKALYDFEMKDKDQDKDCLT
  FTKDEILTVLRRVDENWAEGMLGDKIGIFPLLYVELNDSAKQLIEMDKPCPAAASSCNASLPSDSGAVAS
  VAPSPTLSSSGAVSAFQRRVDGKKNTKKRHSFTALSVTHRSSQAASHRHSMEISAPVLISSSDPRAAARI
  GDLAHLSCAAPTQDVSSSAGSTPTAVPRAASVSGEQGTPPKVQLPLNVYLALYAYKPQKSDELELHKGEM
  YRVLEKCQDGWFKGASLRTGVSGVFPGNYVTPVSRVPAGGAGPPRNNVVGGSPLAKGITTTMHPGSGSLS
  SLATATRPALPITTPQAHAQHPTASPPTGSCLRHSAQPTASQARSTISTAAHSAAQAQDRPTATVSPLRT
  QNSPSRLPATSLRPHSVVSPQHSHQPPVQMCPRPAIPLTSAASAITPPNVSAANLNGEAGGGPIGVLSTS
  SPTNTGCKLDEKKSEKKEKKSGLLKLLAGASTKKKSRSPPSVSPTHDPQVAVDALLQGAVGPEVSSLSIH
  GRAGSCPIESEMQGAMGMEPLHRKAGSLDLNFTSPSRQAPLSMAAIRPEPKLLPRERYRVVVSYPPQSEA
  EIELKEGDIVFVHKKREDGWYKGTLQRNGRTGLFPGSFVESF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001099289
• ORF Size: 2646 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001099289.3
• RefSeq Size: 5608 bp
• RefSeq ORF: 2649 bp
• Locus ID: 344558
• UniProt ID: Q8TEJ3
• Cytogenetics: 2q13
• MW: 92.6 kDa
• Gene Summary: Has E3 ubiquitin-protein ligase activity.[UniProtKB/Swiss-Prot Function]