CRYBB3 (NM_004076) Human Tagged ORF Clone

CAT#: RC216611

CRYBB3 (Myc-DDK-tagged)-Human crystallin, beta B3 (CRYBB3)

Product Images

Circular map for RC216611

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  Western blot validation of overexpression lysate (Cat# LY418242) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216611 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CRYBB3 protein (Cat# TP316611). The protein was produced from HEK293T cells transfected with CRYBB3 cDNA clone (Cat# RC216611) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRYBB3
• Synonyms: CATCN2; CRYB3; CTRCT22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216611 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAACAGCACGGAGCACCCGAACAGGCTGCAGCTGGCAAGAGCCATGGAGACCTTGGGGGCAGCT
  ACAAGGTGATCTTGTACGAACTAGAGAACTTCCAAGGCAAACGCTGCGAGCTCTCGGCCGAGTGCCCCAG
  CCTGACCGACAGCCTGCTGGAGAAGGTGGGCTCCATCCAAGTGGAGTCCGGGCCGTGGCTGGCATTTGAG
  TCCAGGGCCTTCCGCGGGGAGCAGTTTGTTCTGGAGAAGGGGGATTATCCTCGCTGGGATGCCTGGTCCA
  ACAGCCGTGATAGTGACAGCCTTCTGTCCCTCCAGCCTCTGAATATTGATAGTCCAGATCACAAGCTGCA
  TCTGTTTGAGAACCCAGCTTTCAGTGGCCGCAAGATGGAGATAGTGGATGATGACGTGCCCAGCCTGTGG
  GCTCATGGCTTCCAGGACCGTGTGGCGAGTGTCCGTGCCATCAACGGGACGTGGGTTGGCTATGAGTTCC
  CCGGCTACCGTGGGCGCCAGTACGTGTTTGAGCGGGGCGAGTACCGCCACTGGAATGAGTGGGACGCCAG
  CCAGCCGCAGCTGCAGTCTGTGCGCCGCATCCGTGACCAGAAGTGGCACAAGCGGGGCCGCTTCCCCAGC
  AGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216611 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEQHGAPEQAAAGKSHGDLGGSYKVILYELENFQGKRCELSAECPSLTDSLLEKVGSIQVESGPWLAFE
  SRAFRGEQFVLEKGDYPRWDAWSNSRDSDSLLSLQPLNIDSPDHKLHLFENPAFSGRKMEIVDDDVPSLW
  AHGFQDRVASVRAINGTWVGYEFPGYRGRQYVFERGEYRHWNEWDASQPQLQSVRRIRDQKWHKRGRFPS
  S
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004076
• ORF Size: 633 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004076.5
• RefSeq Size: 896 bp
• RefSeq ORF: 636 bp
• Locus ID: 1417
• UniProt ID: P26998
• Cytogenetics: 22q11.23
• MW: 24.2 kDa
• Gene Summary: Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension (present in the basic group, none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene, a beta basic group member, is part of a gene cluster with beta-A4, beta-B1, and beta-B2. Mutations in this gene result in cataract congenital nuclear autosomal recessive type 2. [provided by RefSeq, Feb 2013]