PI4KB (NM_002651) Human Tagged ORF Clone

CAT#: RC216570

PI4KB (Myc-DDK-tagged)-Human phosphatidylinositol 4-kinase, catalytic, beta (PI4KB), transcript variant 1

Product Images

Circular map for RC216570

• 

  Coomassie blue staining of purified PI4KB protein (Cat# TP316570). The protein was produced from HEK293T cells transfected with PI4KB cDNA clone (Cat# RC216570) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PI4KB
• Synonyms: NPIK; PI4K-BETA; PI4K92; PI4KBETA; PI4KIII; PI4KIIIBETA; PIK4CB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216570 representing NM_002651
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGATTCTTGGAAGCTCGAAGTCTGGCTGTGGCCATGGGAGATACAGTAGTGGAGCCTGCCCCCTTGA
  AGCCAACTTCTGAGCCCACTTCTGGCCCACCAGGGAATAATGGGGGGTCCCTGCTAAGTGTCATCACGGA
  GGGGGTCGGGGAACTATCAGTGATTGACCCTGAGGTGGCCCAGAAGGCCTGCCAGGAGGTGTTGGAGAAA
  GTCAAGCTTTTGCATGGAGGCGTGGCAGTCTCTAGCAGAGGCACCCCACTGGAGTTGGTCAATGGGGATG
  GTGTGGACAGTGAGATCCGTTGCCTAGATGATCCACCTGCCCAGATCAGGGAGGAGGAAGATGAGATGGG
  GGCCGCTGTGGCCTCAGGCACAGCCAAAGGAGCAAGAAGACGGCGGCAGAACAACTCAGCTAAACAGTCT
  TGGCTGCTGAGGCTGTTTGAGTCAAAACTGTTTGACATCTCCATGGCCATTTCATACCTGTATAACTCCA
  AGGAGCCTGGAGTACAAGCCTACATTGGCAACCGGCTCTTCTGCTTTCGCAACGAGGACGTGGACTTCTA
  TCTGCCCCAGTTGCTTAACATGTACATCCACATGGATGAGGACGTGGGTGATGCCATTAAGCCCTACATA
  GTCCACCGTTGCCGCCAGAGCATTAACTTTTCCCTCCAGTGTGCCCTGTTGCTTGGGGCCTATTCTTCAG
  ACATGCACATTTCCACTCAACGACACTCCCGTGGGACCAAGCTACGGAAGCTGATCCTCTCAGATGAGCT
  AAAGCCAGCTCACAGGAAGAGGGAGCTGCCCTCCTTGAGCCCGGCCCCTGACACAGGGCTGTCTCCCTCC
  AAAAGGACTCACCAGCGCTCTAAGTCAGATGCCACTGCCAGCATAAGTCTCAGCAGCAACCTGAAACGAA
  CAGCCAGCAACCCTAAAGTGGAGAATGAGGATGAGGAGCTCTCCTCCAGCACCGAGAGTATTGATAATTC
  ATTCAGTTCCCCTGTTCGACTGGCTCCTGAGAGAGAATTCATCAAGTCCCTGATGGCGATCGGCAAGCGG
  CTGGCCACGCTCCCCACCAAAGAGCAGAAAACACAGAGGCTGATCTCAGAGCTCTCCCTGCTCAACCATA
  AGCTCCCTGCCCGAGTCTGGCTGCCCACTGCTGGCTTTGACCACCACGTGGTCCGTGTACCCCACACACA
  GGCTGTTGTCCTCAACTCCAAGGACAAGGCTCCCTACCTGATTTATGTGGAAGTCCTTGAATGTGAAAAC
  TTTGACACCACCAGTGTCCCTGCCCGGATCCCCGAGAACCGAATTCGGAGTACGAGGTCCGTAGAAAACT
  TGCCCGAATGTGGTATTACCCATGAGCAGCGAGCTGGCAGCTTCAGCACTGTGCCCAACTATGACAACGA
  TGATGAGGCCTGGTCGGTGGATGACATAGGCGAGCTGCAAGTGGAGCTCCCCGAAGTGCATACCAACAGC
  TGTGACAACATCTCCCAGTTCTCTGTGGACAGCATCACCAGCCAGGAGAGCAAGGAGCCTGTGTTCATTG
  CAGCAGGGGACATCCGCCGGCGCCTTTCGGAACAGCTGGCTCATACCCCGACAGCCTTCAAACGAGACCC
  AGAAGATCCTTCTGCAGTTGCTCTCAAAGAGCCCTGGCAGGAGAAAGTACGGCGGATCAGAGAGGGCTCC
  CCCTACGGCCATCTCCCCAATTGGCGGCTCCTGTCAGTCATTGTCAAGTGTGGGGATGACCTTCGGCAAG
  AGCTTCTGGCCTTTCAGGTGTTGAAGCAACTGCAGTCCATTTGGGAACAGGAGCGAGTGCCCCTTTGGAT
  CAAGCCATACAAGATTCTTGTGATTTCGGCTGATAGTGGCATGATTGAACCAGTGGTCAATGCTGTGTCC
  ATCCATCAGGTGAAGAAACAGTCACAGCTCTCCTTGCTCGATTACTTCCTACAGGAGCACGGCAGTTACA
  CCACTGAGGCATTCCTCAGTGCACAGCGCAATTTTGTGCAAAGTTGTGCTGGGTACTGCTTGGTCTGCTA
  CCTGCTGCAAGTCAAGGACAGACACAATGGGAATATCCTTTTGGACGCAGAAGGCCACATCATCCACATC
  GACTTTGGCTTCATCCTCTCCAGCTCACCCCGAAATCTGGGCTTTGAGACGTCAGCCTTTAAGCTGACCA
  CAGAGTTTGTGGATGTGATGGGCGGCCTGGATGGCGACATGTTCAACTACTATAAGATGCTGATGCTGCA
  AGGGCTGATTGCCGCTCGGAAACACATGGACAAGGTGGTGCAGATCGTGGAGATCATGCAGCAAGGTTCT
  CAGCTTCCTTGCTTCCATGGCTCCAGCACCATTCGAAACCTCAAAGAGAGGTTCCACATGAGCATGACTG
  AGGAGCAGCTGCAGCTGCTGGTGGAGCAGATGGTGGATGGCAGTATGCGGTCTATCACCACCAAACTCTA
  TGACGGCTTCCAGTACCTCACCAACGGCATCATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216570 representing NM_002651
  Red=Cloning site Green=Tags(s)
  
  MRFLEARSLAVAMGDTVVEPAPLKPTSEPTSGPPGNNGGSLLSVITEGVGELSVIDPEVAQKACQEVLEK
  VKLLHGGVAVSSRGTPLELVNGDGVDSEIRCLDDPPAQIREEEDEMGAAVASGTAKGARRRRQNNSAKQS
  WLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFCFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYI
  VHRCRQSINFSLQCALLLGAYSSDMHISTQRHSRGTKLRKLILSDELKPAHRKRELPSLSPAPDTGLSPS
  KRTHQRSKSDATASISLSSNLKRTASNPKVENEDEELSSSTESIDNSFSSPVRLAPEREFIKSLMAIGKR
  LATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECEN
  FDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNS
  CDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQLAHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGS
  PYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVS
  IHQVKKQSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHI
  DFGFILSSSPRNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGS
  QLPCFHGSSTIRNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGIM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002651
• ORF Size: 2484 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002651.4
• RefSeq Size: 3471 bp
• RefSeq ORF: 2487 bp
• Locus ID: 5298
• UniProt ID: Q9UBF8
• Cytogenetics: 1q21.3
• Domains: PI3_PI4_kinase
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 92.5 kDa
• Gene Summary: Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity).[UniProtKB/Swiss-Prot Function]