PI4KB (NM_002651) Human Untagged Clone

CAT#: SC118488

PI4KB (untagged)-Human phosphatidylinositol 4-kinase, catalytic, beta (PI4KB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PI4KB
• Synonyms: NPIK; PI4K-BETA; PI4K92; PI4KBETA; PI4KIII; PI4KIIIBETA; PIK4CB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002651, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGATTCTTGGAAGCTCGAAGTCTGGCTGTGGCCATGGGAGATACAGTAGTGGAGCCTGCCCCCTTGA
  AGCCAACTTCTGAGCCCACTTCTGGCCCACCAGGGAATAATGGGGGGTCCCTGCTAAGTGTCATCACGGA
  GGGGGTCGGGGAACTATCAGTGATTGACCCTGAGGTGGCCCAGAAGGCCTGCCAGGAGGTGTTGGAGAAA
  GTCAAGCTTTTGCATGGAGGCGTGGCAGTCTCTAGCAGAGGCACCCCACTGGAGTTGGTCAATGGGGATG
  GTGTGGACAGTGAGATCCGTTGCCTAGATGATCCACCTGCCCAGATCAGGGAGGAGGAAGATGAGATGGG
  GGCCGCTGTGGCCTCAGGCACAGCCAAAGGAGCAAGAAGACGGCGGCAGAACAACTCAGCTAAACAGTCT
  TGGCTGCTGAGGCTGTTTGAGTCAAAACTGTTTGACATCTCCATGGCCATTTCATACCTGTATAACTCCA
  AGGAGCCTGGAGTACAAGCCTACATTGGCAACCGGCTCTTCTGCTTTCGCAACGAGGACGTGGACTTCTA
  TCTGCCCCAGTTGCTTAACATGTACATCCACATGGATGAGGACGTGGGTGATGCCATTAAGCCCTACATA
  GTCCACCGTTGCCGCCAGAGCATTAACTTTTCCCTCCAGTGTGCCCTGTTGCTTGGGGCCTATTCTTCAG
  ACATGCACATTTCCACTCAACGACACTCCCGTGGGACCAAGCTACGGAAGCTGATCCTCTCAGATGAGCT
  AAAGCCAGCTCACAGGAAGAGGGAGCTGCCCTCCTTGAGCCCGGCCCCTGACACAGGGCTGTCTCCCTCC
  AAAAGGACTCACCAGCGCTCTAAGTCAGATGCCACTGCCAGCATAAGTCTCAGCAGCAACCTGAAACGAA
  CAGCCAGCAACCCTAAAGTGGAGAATGAGGATGAGGAGCTCTCCTCCAGCACCGAGAGTATTGATAATTC
  ATTCAGTTCCCCTGTTCGACTGGCTCCTGAGAGAGAATTCATCAAGTCCCTGATGGCGATCGGCAAGCGG
  CTGGCCACGCTCCCCACCAAAGAGCAGAAAACACAGAGGCTGATCTCAGAGCTCTCCCTGCTCAACCATA
  AGCTCCCTGCCCGAGTCTGGCTGCCCACTGCTGGCTTTGACCACCACGTGGTCCGTGTACCCCACACACA
  GGCTGTTGTCCTCAACTCCAAGGACAAGGCTCCCTACCTGATTTATGTGGAAGTCCTTGAATGTGAAAAC
  TTTGACACCACCAGTGTCCCTGCCCGGATCCCCGAGAACCGAATTCGGAGTACGAGGTCCGTAGAAAACT
  TGCCCGAATGTGGTATTACCCATGAGCAGCGAGCTGGCAGCTTCAGCACTGTGCCCAACTATGACAACGA
  TGATGAGGCCTGGTCGGTGGATGACATAGGCGAGCTGCAAGTGGAGCTCCCCGAAGTGCATACCAACAGC
  TGTGACAACATCTCCCAGTTCTCTGTGGACAGCATCACCAGCCAGGAGAGCAAGGAGCCTGTGTTCATTG
  CAGCAGGGGACATCCGCCGGCGCCTTTCGGAACAGCTGGCTCATACCCCGACAGCCTTCAAACGAGACCC
  AGAAGATCCTTCTGCAGTTGCTCTCAAAGAGCCCTGGCAGGAGAAAGTACGGCGGATCAGAGAGGGCTCC
  CCCTACGGCCATCTCCCCAATTGGCGGCTCCTGTCAGTCATTGTCAAGTGTGGGGATGACCTTCGGCAAG
  AGCTTCTGGCCTTTCAGGTGTTGAAGCAACTGCAGTCCATTTGGGAACAGGAGCGAGTGCCCCTTTGGAT
  CAAGCCATACAAGATTCTTGTGATTTCGGCTGATAGTGGCATGATTGAACCAGTGGTCAATGCTGTGTCC
  ATCCATCAGGTGAAGAAACAGTCACAGCTCTCCTTGCTCGATTACTTCCTACAGGAGCACGGCAGTTACA
  CCACTGAGGCATTCCTCAGTGCACAGCGCAATTTTGTGCAAAGTTGTGCTGGGTACTGCTTGGTCTGCTA
  CCTGCTGCAAGTCAAGGACAGACACAATGGGAATATCCTTTTGGACGCAGAAGGCCACATCATCCACATC
  GACTTTGGCTTCATCCTCTCCAGCTCACCCCGAAATCTGGGCTTTGAGACGTCAGCCTTTAAGCTGACCA
  CAGAGTTTGTGGATGTGATGGGCGGCCTGGATGGCGACATGTTCAACTACTATAAGATGCTGATGCTGCA
  AGGGCTGATTGCCGCTCGGAAACACATGGACAAGGTGGTGCAGATCGTGGAGATCATGCAGCAAGGTTCT
  CAGCTTCCTTGCTTCCATGGCTCCAGCACCATTCGAAACCTCAAAGAGAGGTTCCACATGAGCATGACTG
  AGGAGCAGCTGCAGCTGCTGGTGGAGCAGATGGTGGATGGCAGTATGCGGTCTATCACCACCAAACTCTA
  TGACGGCTTCCAGTACCTCACCAACGGCATCATGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002651 unedited
  NGGGGGGTCAAATTTGTACACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGATTTT
  ATTTTATTTTATTATTTTTTTGGTCTTGGTTGTACTGAGGGAGGAAGAAGAGGTTGTGTG
  GCCCGGTCGAACTTGTGGCAGCCTGAAGGCCCCCTCAGGCGGCGCCGCGGGCAGCCCCGC
  AGCCGGGGCCTGGTGCAGCCTCCGCGGCCGCTGTCAGGGAAGCGCAGGCGGCCAATGGAA
  CCCGGGAGCGGTCGCTGCTGCTGAGGCGGCAGTGTCGGCAGTCCAACCGCGACTGCCCGC
  ACCCCCTCCGCGGGGGTCCCCCAGAGGATCAACTAAACCTTGAACTAAGAAGAAAAATGT
  GTTGTGAGCAGGGGGAGCCTCAGCTGCCTCAGGCCGTTCAGGACAGAAGGGTGTTTCTGA
  AGGCCGGAGCAAGTTTTGAAGAAGTCCCTATCAGATTACACTTGGTTGACTACTCCGGAG
  CAGCCACTAAGAGGGATGAACAGGCCTGCGTGGAAATTGAATGAGATTCTTGGAAGCTCG
  AAGTCTGGCTGTGGCCATGGGAGATACAGTAGTGGAGCCTGCCCCCTTGAAGCCAACTTC
  TGAGCCCACTTCTGGCCCACCAGGGAATAATGGGGGGTCCCTGCTAAGTGTCATCACGGA
  GGGGGTCGGNGAACTATCAGTGATTGACCCTTGAGTGGCCCAGAAGCCTGCCAGGAGGTG
  TTGGAGAAAGTCAAGCTTTTGCATGGAGGCGTGGCAGTCTCTAGCAGAGGCACCCACTGG
  GAGTTGTCATGGGGGATGGTGTGGACCGTGANTATCCGTGCCTANATGATCCACCTGCCC
  AATCAGGGAGGAGGAATAGATTAGGCGGCCCGTTTTGCCCTAGGCCACTCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002651 unedited
  TAGAGGCATTTTTTCAACAAAATTAAAAATAAAAAATTGANAGCTCTTTNNTCCCTGGGT
  TTGGGNGAAGGGGAGTCAGGGTAGGGGAATTCCACATGGCTAGGCCAGTACCCTCAGTAC
  ACTGGAGGGCTGTGGGAATTCCCTTCCTTGCCCATGATGGGGAACCTCAGCAAGTGCAGG
  GCCCACGCAGCGCCCGCCAGCAAGGGGAGCTTGGGCCAGGGGTCGGTCCCTGGGAGGGGT
  AGGTCCCTGGGAGGGGTAGAGCTGGGCAGCAGCATGGCCAGTGGCCAACCACAGGATCAA
  GTCCTAAGTACCGAGAACCTACTCTTCCCCACTTCCTGGGCCCCAGAACGGAATAAGAGG
  ATGGCCTGTGGAAAGAACCTCTGAGAGACCCCTACAAGGAGAAGAAAGGCCCCAGTGTCC
  CTCACATTTGTCACCTCTGTTTTCTGGAGGGCAGCGAGTCCTGGAGTCAGTCTGGTGGTA
  ATACTGACACAGACCAGGAGGGGCATGGAAGCCCCAACAAGTCTGGACCCCACAGCTGGC
  TCTCCCACCCCTTAGCAAGCTCTCGCAGTTACCACATGATCCTTCGTGCCTCTTGCCCTC
  CATTTCCCTTGGGCGGATGGCTGGCGCAGGGGGGCCTCCTGGCTTCGGCGCTCCCAGAAA
  AGGCCCTCTTAGGAAGGTGCCCTGCACCCCCCCCATTCTGGGACTGCCACTGCGTCCACT
  TATCCTCCTCGTGACCCCTCCACCCCCCCACCCTTCGCGTTTCACACCCCTCCTCCCTTC
  CCTCTTTCTCCCCCTTACTCTCCCTCCCCCTCAACCTCCCATGATCCCACATCTCCGTTC
  CAAACCGTCTGCTTCTCCTCTCCCCACCCATAATCTTCTCAAAAAACTGCCTACCTCCCT
  CTCCCCCTTCTCTCTCCCATCCACCCCCCCACCNTTACCTTCTTTCCCCCCTCTCCCCCC
  TTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002651
• Insert Size: 3780 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002651.1, NP_002642.1
• RefSeq Size: 3471 bp
• RefSeq ORF: 2487 bp
• Locus ID: 5298
• UniProt ID: Q9UBF8
• Cytogenetics: 1q21.3
• Domains: PI3_PI4_kinase
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.