RNF169 (NM_001098638) Human Tagged ORF Clone

CAT#: RC216450

RNF169 (Myc-DDK-tagged)-Human ring finger protein 169 (RNF169)

Product Images

Circular map for RC216450

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RNF169
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216450 representing NM_001098638
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTGCAGGTCCGAGTACTCGGGCCTCTTCCGCGGCGGCAGCAGCCGCTCTGAGTCGGCGGGGCC
  GGCGGGGCCGCTGTGACGAGACGGCGGCAGCTAAGACTGGGGCCCCAGGCCCGGCTTCTGGACCTTCGCT
  GTTGGTGTTGTCGCCGCCGTTGCTGCAGCCGCCGCTGCCGCCGCGGCCGGAGGAATCGGGCTGCGCCGGG
  TGCCTGGAGCCCCCCGGAGAAGCAGCGGCCCTGCCGTGCGGCCACTCGCTTTGCCGAGGCTGCGCCCAAC
  GCGCCGCCGACGCGGCGGGCCCGGGTTGCCCTCGCTGCCGCGCCCGCGGCCCAGGCTGGGCCCGCCGTCG
  GGCCCGCGACGACGGCCAGGCCGACTCAGAGGTGCTGGGCGAGTGCGCCCGCCGCAGCCAACCCGAGCGC
  TGCCGCCCGCGCCGGGACGGGGGCGCGGCTGCCGCGGGGCCCAGGCCAGAGCAGGAGCCGCGTGCCGCGC
  CTGCGGAGCCAGACTTTATATTCAGAGCACCAATCAAATTAAGCAAGCCTGGGGAACTTCGTGAGGAATA
  TGAAAGCTTGAGAAAGCTGAGAGAAGAAAAGTTACAAGAGGAAAAACCCTCTGAAGATCAAATCCACAAG
  CTGTTACCAGAGGATACAGAAACAGGGAAAAGGAAAATGGATGAACAGAAAAAAAGAGATGAACCATTAG
  TACTGAAAACAAATCTGGAACGTTGTCCTGCACGTCTCTCAGATTCAGAGAATGAAGAACCTTCTCGAGG
  CCAGATGACACAGACACATCGCTCGGCATTTGTTTCCAAGAACAACTCCTACTCCTTAGCTTTCCTGGCA
  GGGAAGCTAAACTCCAAGGTGGAAAGGAGTCAGAGCTGTAGTGACACAGCCCAGGAAAGAGCGAAGAGCA
  GAGTCAGAGCAGTTCCAGGCAACAAAGCCAAGGTCACAACTATGACTCCAGCCTCCAACCCCATCATTGG
  TGTCCTCTTGTCAACTCAAAACAACCGCTGCGTCTCGGCCCCTGACTTAACCATCGAAAAGCGTCTACCC
  TTCAGCTCCCTTTCATCCTTGGCTTCCCTGCATAAGCCAGAGCGTTCTGTCAGCCCTGAGAGCAATGACA
  GCATCTCCGAAGAACTAAACCATTTCAAGCCCATTGTCTGCTCACCATGTACTCCTCCCAAGAGACTCCC
  TGATGGCCGTGTGCTAAGTCCTCTCATCATCAAATCAACTCCACGCAACCTAAACAGAAGCCTGCAGAAG
  CAGACTTCTTATGAGGCCAGTCCACGGATCCTCAAAAAGTGGGAACAGATCTTTCAGGAGCGGCAGATCA
  AAAAGACCCTTTCAAAAGCCACTCTTACCTCTCTGGCTCCTGAAATGGGGGAAGAGTTACTAGGCTCTGA
  AGGTATCCATTCTAGCAAGGAGAAGCCACTTGTGGCTGTAAATACAAGATTATCTGGTGGGCAGGTCCTC
  TCTGAGTATACTGGACCCACCTCTGCTGATCTTGATCATTTCCCCTCTGTTAGCCAAACAAAAGCAGAAC
  AGGACAGTGATAATAAAAGTAGCACTGAGATCCCACTGGAAACCTGCTGTTCCTCAGAACTCAAAGGGGG
  AGGCAGTGGGACTTCTTTGGAGAGGGAGCAGTTTGAGGGGTTAGGGTCAACTCCAGATGCCAAGTTAGAC
  AAAACCTGTATAAGCAGAGCCATGAAAATCACCACAGTTAATTCAGTGCTACCCCAAAACAGTGTTTTGG
  GTGGAGTCCTCAAAACAAAGCAACAATTGAAGACATTAAATCATTTTGATCTGACTAATGGTGTTCTAGT
  TGAGAGCCTAAGTGAAGAGCCACTTCCTTCTTTGCGTCGAGGCCGGAAAAGACACTGCAAGACCAAGCAC
  TTAGAACAAAATGGCTCCCTTAAAAAACTGCGACAAACCAGTGGGGAGGTGGGTCTGGCCCCAACAGACC
  CAGTCCTGCGAGAGATGGAGCAGAAGCTTCAGCAAGAGGAAGAAGACCGACAGTTGGCTCTGCAGTTGCA
  GCGCATGTTCGACAATGAGAGGCGGACTGTGAGCCGGCGAAAAGGAAGTGTGGATCAGTATCTCCTACGG
  TCCAGCAACATGGCCGGGGCCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216450 representing NM_001098638
  Red=Cloning site Green=Tags(s)
  
  MAAAGPSTRASSAAAAAALSRRGRRGRCDETAAAKTGAPGPASGPSLLVLSPPLLQPPLPPRPEESGCAG
  CLEPPGEAAALPCGHSLCRGCAQRAADAAGPGCPRCRARGPGWARRRARDDGQADSEVLGECARRSQPER
  CRPRRDGGAAAAGPRPEQEPRAAPAEPDFIFRAPIKLSKPGELREEYESLRKLREEKLQEEKPSEDQIHK
  LLPEDTETGKRKMDEQKKRDEPLVLKTNLERCPARLSDSENEEPSRGQMTQTHRSAFVSKNNSYSLAFLA
  GKLNSKVERSQSCSDTAQERAKSRVRAVPGNKAKVTTMTPASNPIIGVLLSTQNNRCVSAPDLTIEKRLP
  FSSLSSLASLHKPERSVSPESNDSISEELNHFKPIVCSPCTPPKRLPDGRVLSPLIIKSTPRNLNRSLQK
  QTSYEASPRILKKWEQIFQERQIKKTLSKATLTSLAPEMGEELLGSEGIHSSKEKPLVAVNTRLSGGQVL
  SEYTGPTSADLDHFPSVSQTKAEQDSDNKSSTEIPLETCCSSELKGGGSGTSLEREQFEGLGSTPDAKLD
  KTCISRAMKITTVNSVLPQNSVLGGVLKTKQQLKTLNHFDLTNGVLVESLSEEPLPSLRRGRKRHCKTKH
  LEQNGSLKKLRQTSGEVGLAPTDPVLREMEQKLQQEEEDRQLALQLQRMFDNERRTVSRRKGSVDQYLLR
  SSNMAGAK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001098638
• ORF Size: 2124 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098638.2
• RefSeq Size: 7840 bp
• RefSeq ORF: 2127 bp
• Locus ID: 254225
• UniProt ID: Q8NCN4
• Cytogenetics: 11q13.4
• MW: 77 kDa
• Gene Summary: Probable E3 ubiquitin-protein ligase that acts as a negative regulator of double-strand breaks (DSBs) repair following DNA damage. Recruited to DSB repair sites by recognizing and binding ubiquitin catalyzed by RNF168 and competes with TP53BP1 and BRCA1 for association with RNF168-modified chromatin, thereby acting as a negative regulator of DSBs repair. E3 ubiquitin-protein ligase activity is not required for regulation of DSBs repair.[UniProtKB/Swiss-Prot Function]