PITPNM2 (NM_020845) Human Tagged ORF Clone

CAT#: RC216422

PITPNM2 (Myc-DDK-tagged)-Human phosphatidylinositol transfer protein, membrane-associated 2 (PITPNM2)

Product Images

Circular map for RC216422

• 

  Western blot validation of overexpression lysate (Cat# LY412266) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216422 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PITPNM2 protein (Cat# TP316422). The protein was produced from HEK293T cells transfected with PITPNM2 cDNA clone (Cat# RC216422) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PITPNM2
• Synonyms: NIR-3; NIR3; RDGB2; RDGBA2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216422 representing NM_020845
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTATAAAGGAATATCGGATTCCTCTGCCAATGACCGTGGAGGAGTACCGCATCGCCCAGCTGTACA
  TGATACAGAAGAAGAGCCGTAACGAGACATATGGCGAAGGCAGCGGCGTGGAGATCCTGGAGAACCGGCC
  GTACACAGATGGCCCAGGCGGCTCTGGGCAGTACACACACAAGGTGTATCATGTGGGCATGCACATTCCC
  AGCTGGTTCCGCTCCATCCTGCCCAAGGCAGCCCTGCGGGTGGTGGAGGAGTCTTGGAATGCCTACCCCT
  ACACCCGAACCAGGTTCACCTGTCCTTTCGTGGAGAAATTCTCCATCGACATTGAAACCTTTTATAAAAC
  TGATGCTGGAGAAAACCCCGACGTGTTCAACCTCTCTCCTGTGGAAAAGAACCAGCTGACAATCGACTTC
  ATCGACATTGTCAAAGACCCTGTGCCCCACAACGAGTATAAGACAGAAGAGGACCCCAAGCTGTTCCAGT
  CAACCAAGACCCAGCGGGGGCCCCTGTCCGAGAACTGGATCGAGGAGTACAAGAAGCAGGTCTTCCCCAT
  CATGTGCGCATACAAGCTCTGCAAGGTGGAGTTCCGCTACTGGGGCATGCAGTCCAAGATCGAGAGGTTC
  ATCCACGACACCGGACTACGGAGGGTGATGGTGCGGGCTCACCGGCAGGCCTGGTGCTGGCAGGACGAGT
  GGTATGGGCTGAGCATGGAGAACATCCGGGAGCTGGAGAAGGAGGCACAGCTCATGCTTTCCCGTAAGAT
  GGCCCAGTTCAATGAGGATGGTGAGGAGGCCACTGAGCTCGTCAAGCACGAAGCCGTCTCGGACCAGACC
  TCTGGGGAGCCCCCGGAGCCCAGCAGCAGCAATGGGGAGCCCCTAGTGGGGCGCGGCCTCAAGAAACAGT
  GGTCCACATCCTCCAAGTCGTCTCGGTCGTCCAAGCGGGGAGCGAGTCCTTCCCGCCACAGCATCTCAGA
  GTGGAGGATGCAGAGTATTGCCAGGGACTCGGATGAGAGCTCAGATGATGAGTTCTTCGATGCGCACGAG
  GACCTGTCCGACACAGAGGAAATGTTCCCCAAGGACATCACCAAGTGGAGCTCCAATGACCTCATGGACA
  AGATCGAGAGCCCAGAGCCGGAAGACACACAAGATGGTCTGTACCGCCAGGGTGCCCCTGAGTTCAGGGT
  GGCCTCCAGTGTGGAGCAGCTGAACATCATAGAGGACGAGGTTAGCCAGCCGCTGGCTGCACCGCCCTCC
  AAGATCCACGTGCTGCTACTGGTGCTGCACGGAGGCACCATCCTGGACACAGGCGCCGGGGACCCCAGCT
  CCAAGAAGGGCGATGCTAACACCATCGCCAACGTGTTCGACACCGTCATGCGCGTGCACTACCCCAGCGC
  CCTGGGCCGCCTTGCCATCCGCCTGGTGCCCTGCCCGCCCGTCTGCTCTGACGCCTTTGCCCTGGTCTCC
  AACCTCAGCCCCTACAGCCATGACGAAGGCTGTCTGTCCAGCAGTCAGGACCACATTCCCCTGGCTGCCC
  TCCCCCTGCTGGCCACCTCCTCCCCCCAGTACCAGGAGGCAGTTGCCACAGTGATTCAGCGAGCCAACCT
  TGCCTATGGGGACTTCATCAAGTCCCAGGAGGGCATGACCTTCAATGGGCAGGTCTGCCTGATTGGGGAC
  TGCGTCGGGGGCATCCTGGCATTTGATGCCCTGTGCTACAGTAACCAGCCGGTGTCTGAGAGTCAGAGCA
  GCAGCCGCCGGGGCAGCGTGGTCAGCATGCAGGACAATGACCTGCTGTCCCCGGGCATCCTGATGAATGC
  AGCACACTGCTGCGGTGGTGGCGGTGGCGGCGGTGGCGGTGGTGGCAGCAGTGGTGGTGGTGGCAGTAGT
  GGTGGCTCCAGCCTGGAGAGCAGTCGGCACCTGAGCCGAAGCAACGTCGACATCCCCCGCAGCAACGGCA
  CTGAGGACCCCAAAAGGCAACTGCCCCGCAAGAGGAGCGACTCATCCACCTACGAGCTGGATACCATCCA
  GCAGCACCAGGCCTTCCTGTCCAGCCTCCATGCCAGCGTGCTGAGGACTGAGCCCTGCTCACGCCATTCC
  AGCAGCTCCACCATGCTGGATGGCACAGGTGCCCTGGGCAGGTTTGACTTTGAGATCACCGACCTCTTCC
  TCTTCGGGTGCCCGCTGGGGCTGGTCCTGGCCTTGAGGAAGACTGTCATCCCAGCCCTGGATGTTTTCCA
  GCTGCGGCCGGCCTGCCAGCAAGTCTACAACCTCTTCCACCCCGCGGACCCGTCAGCTTCACGCCTGGAG
  CCGCTGCTGGAACGGCGCTTTCACGCCCTGCCGCCTTTCAGCGTCCCCCGCTACCAACGCTACCCGCTGG
  GGGATGGCTGCTCCACGCTGCTGGCGGATGTGCTCCAGACCCACAATGCAGCCTTCCAAGAGCATGGCGC
  CCCCTCCTCGCCGGGCACTGCCCCTGCCAGTCGTGGCTTCCGCCGAGCCAGTGAGATCAGCATCGCCAGC
  CAGGTGTCAGGCATGGCTGAGAGCTACACGGCATCCAGCATCGCCCAGAAGGCCCCCGATGCGCTCAGCC
  ATACCCCCAGCGTCAGGCGTCTGTCCCTGCTCGCCCTGCCCGCCCCCAGCCCCACCACCCCTGGCCCCCA
  CCCTCCAGCCAGGAAGGCAAGCCCTGGCCTGGAGAGGGCCCCTGGCCTCCCTGAGCTGGACATTGGAGAA
  GTCGCTGCAAAGTGGTGGGGCCAGAAGCGGATCGACTACGCCCTGTACTGCCCTGACGCCCTCACGGCCT
  TCCCCACGGTGGCTCTGCCTCACCTCTTCCACGCCAGCTACTGGGAGTCAACAGACGTGGTCTCCTTTCT
  GCTGAGACAGGTCATGAGGCATGACAACTCCAGCATCTTGGAGCTGGATGGCAAGGAAGTGTCGGTGTTC
  ACCCCCTCAAAGCCAAGGGAGAAGTGGCAGCGCAAGCGGACCCACGTGAAGCTGCGGAACGTGACGGCCA
  ACCACCGGATCAATGATGCCCTTGCCAATGAGGACGGCCCCCAGGTTCTGACGGGCAGGTTCATGTATGG
  GCCCCTGGACATGGTCACCCTGACTGGGGAGAAGGTGGATGTGCACATCATGACCCAGCCGCCCTCAGGC
  GAGTGGCTCTACCTGGATACGCTGGTGACCAACAACAGTGGGCGTGTCTCCTACACCATCCCTGAGTCGC
  ACCGCCTGGGCGTGGGTGTCTACCCTATCAAGATGGTGGTCAGGGGAGACCACACGTTTGCCGACAGCTA
  CATCACCGTGCTGCCCAAGGGCACAGAGTTCGTGGTCTTCAGCATCGACGGTTCCTTTGCCGCTAGCGTG
  TCCATCATGGGCAGCGACCCCAAGGTGCGGGCCGGGGCCGTGGACGTGGTGCGGCACTGGCAGGACCTGG
  GCTACCTCATCATCTACGTGACGGGCCGGCCCGACATGCAGAAGCAGCGGGTGGTGGCGTGGCTGGCCCA
  GCACAACTTCCCCCATGGCGTGGTGTCCTTCTGTGACGGCCTGGTGCATGACCCGCTGCGGCACAAGGCC
  AACTTCCTGAAGCTGCTCATCTCCGAGCTGCACCTGCGCGTGCACGCGGCCTATGGCTCCACCAAGGACG
  TGGCGGTGTACAGCGCCATTAGCCTGTCCCCCATGCAGATCTACATCGTGGGCCGGCCCACCAAGAAGCT
  GCAGCAGCAGTGCCAGTTCATCACGGATGGCTACGCGGCCCACCTGGCGCAGCTGAAGTACAGCCACCGG
  GCGCGGCCCGCTCGCAACACGGCCACCCGCATGGCGCTGCGCAAGGGCAGCTTCGGCCTGCCCGGCCAGG
  GCGACTTTCTGCGCTCCCGGAACCACCTGCTTCGCACCATCTCGGCCCAGCCCAGCGGGCCCAGCCACCG
  GCACGAGCGGACACAGAGCCAGGCGGATGGCGAGCAGCGGGGCCAGCGCAGCATGAGTGTGGCGGCCGGC
  TGCTGGGGCCGCGCCATGACTGGCCGCCTGGAGCCGGGGGCAGCCGCGGGCCCCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216422 representing NM_020845
  Red=Cloning site Green=Tags(s)
  
  MIIKEYRIPLPMTVEEYRIAQLYMIQKKSRNETYGEGSGVEILENRPYTDGPGGSGQYTHKVYHVGMHIP
  SWFRSILPKAALRVVEESWNAYPYTRTRFTCPFVEKFSIDIETFYKTDAGENPDVFNLSPVEKNQLTIDF
  IDIVKDPVPHNEYKTEEDPKLFQSTKTQRGPLSENWIEEYKKQVFPIMCAYKLCKVEFRYWGMQSKIERF
  IHDTGLRRVMVRAHRQAWCWQDEWYGLSMENIRELEKEAQLMLSRKMAQFNEDGEEATELVKHEAVSDQT
  SGEPPEPSSSNGEPLVGRGLKKQWSTSSKSSRSSKRGASPSRHSISEWRMQSIARDSDESSDDEFFDAHE
  DLSDTEEMFPKDITKWSSNDLMDKIESPEPEDTQDGLYRQGAPEFRVASSVEQLNIIEDEVSQPLAAPPS
  KIHVLLLVLHGGTILDTGAGDPSSKKGDANTIANVFDTVMRVHYPSALGRLAIRLVPCPPVCSDAFALVS
  NLSPYSHDEGCLSSSQDHIPLAALPLLATSSPQYQEAVATVIQRANLAYGDFIKSQEGMTFNGQVCLIGD
  CVGGILAFDALCYSNQPVSESQSSSRRGSVVSMQDNDLLSPGILMNAAHCCGGGGGGGGGGGSSGGGGSS
  GGSSLESSRHLSRSNVDIPRSNGTEDPKRQLPRKRSDSSTYELDTIQQHQAFLSSLHASVLRTEPCSRHS
  SSSTMLDGTGALGRFDFEITDLFLFGCPLGLVLALRKTVIPALDVFQLRPACQQVYNLFHPADPSASRLE
  PLLERRFHALPPFSVPRYQRYPLGDGCSTLLADVLQTHNAAFQEHGAPSSPGTAPASRGFRRASEISIAS
  QVSGMAESYTASSIAQKAPDALSHTPSVRRLSLLALPAPSPTTPGPHPPARKASPGLERAPGLPELDIGE
  VAAKWWGQKRIDYALYCPDALTAFPTVALPHLFHASYWESTDVVSFLLRQVMRHDNSSILELDGKEVSVF
  TPSKPREKWQRKRTHVKLRNVTANHRINDALANEDGPQVLTGRFMYGPLDMVTLTGEKVDVHIMTQPPSG
  EWLYLDTLVTNNSGRVSYTIPESHRLGVGVYPIKMVVRGDHTFADSYITVLPKGTEFVVFSIDGSFAASV
  SIMGSDPKVRAGAVDVVRHWQDLGYLIIYVTGRPDMQKQRVVAWLAQHNFPHGVVSFCDGLVHDPLRHKA
  NFLKLLISELHLRVHAAYGSTKDVAVYSAISLSPMQIYIVGRPTKKLQQQCQFITDGYAAHLAQLKYSHR
  ARPARNTATRMALRKGSFGLPGQGDFLRSRNHLLRTISAQPSGPSHRHERTQSQADGEQRGQRSMSVAAG
  CWGRAMTGRLEPGAAAGPK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020845
• ORF Size: 4047 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020845.2, NP_065896.1
• RefSeq Size: 6754 bp
• RefSeq ORF: 4050 bp
• Locus ID: 57605
• UniProt ID: Q9BZ72
• Cytogenetics: 12q24.31
• Protein Families: Druggable Genome
• MW: 148.8 kDa
• Gene Summary: PITPNM2 belongs to a family of membrane-associated phosphatidylinositol transfer domain-containing proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein (Ocaka et al., 2005 [PubMed 15627748]).[supplied by OMIM, Mar 2008]