SLC8A2 (NM_015063) Human Tagged ORF Clone

CAT#: RC216354

SLC8A2 (Myc-DDK-tagged)-Human solute carrier family 8 (sodium/calcium exchanger), member 2 (SLC8A2)

Product Images

Circular map for RC216354

• 

  Western blot validation of overexpression lysate (Cat# LY414804) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216354 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC8A2
• Synonyms: NCX2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216354 representing NM_015063
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCCCCTGGCCTTGGTGGGGGTCACACTCCTCCTGGCGGCTCCCCCATGCTCCGGGGCAGCCACCC
  CAACCCCCTCCCTGCCGCCTCCCCCGGCCAATGACAGCGACACCAGCACAGGGGGCTGCCAGGGGTCCTA
  CCGCTGCCAGCCGGGGGTGCTGCTGCCCGTGTGGGAGCCCGACGACCCGTCGCTGGGTGACAAGGCGGCA
  CGGGCAGTGGTGTACTTTGTGGCCATGGTCTACATGTTTCTGGGAGTGTCCATCATCGCCGACCGTTTCA
  TGGCGGCCATCGAGGTCATCACGTCAAAAGAGAAGGAGATCACCATCACCAAGGCCAACGGTGAGACCAG
  CGTGGGCACCGTTCGCATCTGGAATGAGACGGTGTCCAACCTCACGCTCATGGCCCTGGGCTCCTCCGCA
  CCTGAGATCCTGCTGTCAGTCATCGAAGTCTGCGGCCACAACTTCCAGGCGGGTGAGCTGGGCCCAGGCA
  CCATCGTGGGCAGCGCTGCCTTCAACATGTTTGTGGTCATCGCCGTGTGCATCTACGTCATCCCAGCCGG
  CGAGAGCCGCAAGATCAAGCACCTGAGAGTCTTCTTTGTCACTGCCTCTTGGAGCATCTTCGCCTATGTC
  TGGCTTTATCTCATCCTTGCTGTTTTTTCCCCCGGTGTGGTCCAGGTGTGGGAGGCGCTGCTGACCCTGG
  TCTTCTTCCCGGTGTGCGTGGTATTCGCCTGGATGGCCGACAAGCGGCTGCTCTTCTACAAGTACGTGTA
  CAAGCGCTACCGCACCGACCCACGCAGCGGCATCATCATAGGCGCCGAGGGCGACCCCCCGAAGAGCATC
  GAGCTGGACGGCACGTTCGTGGGCGCCGAGGCCCCAGGTGAGCTGGGCGGCCTGGGCCCGGGCCCCGCCG
  AGGCGCGCGAGCTGGACGCCAGCCGCCGCGAGGTCATCCAGATCCTCAAGGACCTCAAGCAGAAGCACCC
  GGACAAGGATCTGGAGCAGCTGGTGGGCATCGCCAACTACTACGCGCTGCTGCACCAGCAGAAGAGCCGC
  GCCTTCTACCGCATCCAGGCCACGCGGCTGATGACCGGCGCCGGGAACGTGCTGCGCAGACACGCGGCGG
  ACGCCTCGCGCAGGGCGGCGCCGGCCGAGGGCGCGGGCGAGGACGAAGACGACGGCGCCAGCCGCATCTT
  CTTCGAGCCTAGCCTCTACCACTGCCTGGAGAACTGCGGCTCCGTGCTGCTGTCCGTCACGTGCCAGGGC
  GGCGAGGGCAACAGCACCTTCTACGTGGACTACCGCACTGAGGACGGCTCTGCCAAGGCGGGCTCCGACT
  ACGAGTACAGCGAGGGCACGCTGGTGTTCAAACCAGGCGAGACGCAGAAGGAGCTGCGCATCGGCATCAT
  CGACGACGACATCTTCGAGGAGGACGAGCATTTCTTCGTGCGGCTGCTGAACCTGCGCGTGGGCGACGCG
  CAGGGCATGTTCGAGCCGGACGGCGGCGGGCGGCCCAAGGGGCGGCTGGTGGCGCCGCTGCTGGCCACCG
  TCACCATCCTGGACGACGACCACGCAGGCATCTTCTCCTTCCAGGACCGCCTGCTGCACGTGAGCGAGTG
  CATGGGCACCGTGGACGTGCGCGTCGTGCGCAGCTCGGGCGCGCGCGGCACCGTGCGCCTTCCCTACCGC
  ACGGTGGACGGCACGGCGCGCGGCGGCGGCGTGCACTACGAGGACGCGTGCGGAGAGCTGGAGTTTGGCG
  ACGACGAGACCATGAAAACTCTTCAGGTGAAGATAGTTGATGACGAGGAATATGAGAAAAAGGATAATTT
  CTTCATTGAGCTGGGCCAGCCCCAGTGGCTTAAGCGAGGGATTTCAGCTCTGCTACTCAATCAAGGGGAT
  GGGGACAGGAAGCTAACAGCCGAGGAGGAGGAGGCTCGGAGGATAGCAGAGATGGGCAAGCCAGTTCTTG
  GGGAGAACTGCCGGCTGGAGGTCATCATCGAGGAGTCATATGATTTTAAGAACACGGTGGATAAACTCAT
  CAAGAAAACGAACTTGGCCTTGGTAATTGGGACCCATTCATGGAGGGAGCAGTTTTTAGAGGCAATTACG
  GTGAGCGCAGGGGACGAGGAGGAGGAGGAGGACGGGTCCCGGGAGGAGCGGCTGCCGTCGTGCTTTGACT
  ACGTGATGCACTTCCTGACGGTGTTCTGGAAGGTGCTCTTCGCCTGTGTGCCCCCCACCGAGTACTGCCA
  CGGCTGGGCCTGCTTTGGTGTCTCCATCCTGGTCATCGGCCTGCTCACCGCCCTCATTGGGGACCTCGCC
  TCCCACTTCGGCTGCACCGTTGGCCTCAAGGACTCTGTCAATGCTGTTGTCTTCGTTGCCCTGGGCACCT
  CCATCCCTGACACGTTCGCCAGCAAGGTGGCGGCGCTGCAGGACCAGTGCGCCGACGCGTCCATCGGCAA
  CGTGACCGGCTCCAACGCGGTGAACGTGTTCCTTGGCCTGGGCGTCGCCTGGTCTGTGGCCGCCGTGTAC
  TGGGCGGTGCAGGGCCGCCCCTTCGAGGTGCGCACTGGCACGCTGGCCTTCTCCGTCACGCTCTTCACCG
  TCTTCGCCTTCGTGGGCATTGCCGTGCTGCTGTACCGGCGCCGGCCGCACATCGGCGGCGAGCTGGGCGG
  CCCGCGCGGACCCAAGCTCGCCACCACCGCGCTCTTCCTGGGCCTCTGGCTCCTGTACATCCTCTTCGCC
  AGCCTGGAGGCGTACTGCCACATCCGGGGCTTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216354 representing NM_015063
  Red=Cloning site Green=Tags(s)
  
  MAPLALVGVTLLLAAPPCSGAATPTPSLPPPPANDSDTSTGGCQGSYRCQPGVLLPVWEPDDPSLGDKAA
  RAVVYFVAMVYMFLGVSIIADRFMAAIEVITSKEKEITITKANGETSVGTVRIWNETVSNLTLMALGSSA
  PEILLSVIEVCGHNFQAGELGPGTIVGSAAFNMFVVIAVCIYVIPAGESRKIKHLRVFFVTASWSIFAYV
  WLYLILAVFSPGVVQVWEALLTLVFFPVCVVFAWMADKRLLFYKYVYKRYRTDPRSGIIIGAEGDPPKSI
  ELDGTFVGAEAPGELGGLGPGPAEARELDASRREVIQILKDLKQKHPDKDLEQLVGIANYYALLHQQKSR
  AFYRIQATRLMTGAGNVLRRHAADASRRAAPAEGAGEDEDDGASRIFFEPSLYHCLENCGSVLLSVTCQG
  GEGNSTFYVDYRTEDGSAKAGSDYEYSEGTLVFKPGETQKELRIGIIDDDIFEEDEHFFVRLLNLRVGDA
  QGMFEPDGGGRPKGRLVAPLLATVTILDDDHAGIFSFQDRLLHVSECMGTVDVRVVRSSGARGTVRLPYR
  TVDGTARGGGVHYEDACGELEFGDDETMKTLQVKIVDDEEYEKKDNFFIELGQPQWLKRGISALLLNQGD
  GDRKLTAEEEEARRIAEMGKPVLGENCRLEVIIEESYDFKNTVDKLIKKTNLALVIGTHSWREQFLEAIT
  VSAGDEEEEEDGSREERLPSCFDYVMHFLTVFWKVLFACVPPTEYCHGWACFGVSILVIGLLTALIGDLA
  SHFGCTVGLKDSVNAVVFVALGTSIPDTFASKVAALQDQCADASIGNVTGSNAVNVFLGLGVAWSVAAVY
  WAVQGRPFEVRTGTLAFSVTLFTVFAFVGIAVLLYRRRPHIGGELGGPRGPKLATTALFLGLWLLYILFA
  SLEAYCHIRGF
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015063
• ORF Size: 2763 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015063.3
• RefSeq Size: 4282 bp
• RefSeq ORF: 2766 bp
• Locus ID: 6543
• UniProt ID: Q9UPR5
• Cytogenetics: 19q13.32
• Protein Families: Transmembrane
• Protein Pathways: Calcium signaling pathway
• MW: 100.2 kDa
• Gene Summary: Mediates the electrogenic exchange of Ca(2+) against Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes. Contributes to cellular Ca(2+) homeostasis in excitable cells. Contributes to the rapid decrease of cytoplasmic Ca(2+) levels back to baseline after neuronal activation, and thereby contributes to modulate synaptic plasticity, learning and memory. Plays a role in regulating urinary Ca(2+) and Na(+) excretion.[UniProtKB/Swiss-Prot Function]