SLC8A2 (NM_015063) Human Untagged Clone

CAT#: SC304218

SLC8A2 (untagged)-Human solute carrier family 8 (sodium/calcium exchanger), member 2 (SLC8A2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC8A2
• Synonyms: NCX2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304218 representing NM_015063.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCTCCCCTGGCCTTGGTGGGGGTCACACTCCTCCTGGCGGCTCCCCCATGCTCCGGGGCAGCCACC
  CCAACCCCCTCCCTGCCGCCTCCCCCGGCCAATGACAGCGACACCAGCACAGGGGGCTGCCAGGGGTCC
  TACCGCTGCCAGCCGGGGGTGCTGCTGCCCGTGTGGGAGCCCGACGACCCGTCGCTGGGTGACAAGGCG
  GCACGGGCAGTGGTGTACTTTGTGGCCATGGTCTACATGTTTCTGGGAGTGTCCATCATCGCCGACCGT
  TTCATGGCGGCCATCGAGGTCATCACGTCAAAAGAGAAGGAGATCACCATCACCAAGGCCAACGGTGAG
  ACCAGCGTGGGCACCGTTCGCATCTGGAATGAGACGGTGTCCAACCTCACGCTCATGGCCCTGGGCTCC
  TCCGCACCTGAGATCCTGCTGTCAGTCATCGAAGTCTGCGGCCACAACTTCCAGGCGGGTGAGCTGGGC
  CCAGGCACCATCGTGGGCAGCGCTGCCTTCAACATGTTTGTGGTCATCGCCGTGTGCATCTACGTCATC
  CCAGCCGGCGAGAGCCGCAAGATCAAGCACCTGAGAGTCTTCTTTGTCACTGCCTCTTGGAGCATCTTC
  GCCTATGTCTGGCTTTATCTCATCCTTGCTGTTTTTTCCCCCGGTGTGGTCCAGGTGTGGGAGGCGCTG
  CTGACCCTGGTCTTCTTCCCGGTGTGCGTGGTATTCGCCTGGATGGCCGACAAGCGGCTGCTCTTCTAC
  AAGTACGTGTACAAGCGCTACCGCACCGACCCACGCAGCGGCATCATCATAGGCGCCGAGGGCGACCCC
  CCGAAGAGCATCGAGCTGGACGGCACGTTCGTGGGCGCCGAGGCCCCAGGTGAGCTGGGCGGCCTGGGC
  CCGGGCCCCGCCGAGGCGCGCGAGCTGGACGCCAGCCGCCGCGAGGTCATCCAGATCCTCAAGGACCTC
  AAGCAGAAGCACCCGGACAAGGATCTGGAGCAGCTGGTGGGCATCGCCAACTACTACGCGCTGCTGCAC
  CAGCAGAAGAGCCGCGCCTTCTACCGCATCCAGGCCACGCGGCTGATGACCGGCGCCGGGAACGTGCTG
  CGCAGACACGCGGCGGACGCCTCGCGCAGGGCGGCGCCGGCCGAGGGCGCGGGCGAGGACGAAGACGAC
  GGCGCCAGCCGCATCTTCTTCGAGCCTAGCCTCTACCACTGCCTGGAGAACTGCGGCTCCGTGCTGCTG
  TCCGTCACGTGCCAGGGCGGCGAGGGCAACAGCACCTTCTACGTGGACTACCGCACTGAGGACGGCTCT
  GCCAAGGCGGGCTCCGACTACGAGTACAGCGAGGGCACGCTGGTGTTCAAACCAGGCGAGACGCAGAAG
  GAGCTGCGCATCGGCATCATCGACGACGACATCTTCGAGGAGGACGAGCATTTCTTCGTGCGGCTGCTG
  AACCTGCGCGTGGGCGACGCGCAGGGCATGTTCGAGCCGGACGGCGGCGGGCGGCCCAAGGGGCGGCTG
  GTGGCGCCGCTGCTGGCCACCGTCACCATCCTGGACGACGACCACGCAGGCATCTTCTCCTTCCAGGAC
  CGCCTGCTGCACGTGAGCGAGTGCATGGGCACCGTGGACGTGCGCGTCGTGCGCAGCTCGGGCGCGCGC
  GGCACCGTGCGCCTTCCCTACCGCACGGTGGACGGCACGGCGCGCGGCGGCGGCGTGCACTACGAGGAC
  GCGTGCGGAGAGCTGGAGTTTGGCGACGACGAGACCATGAAAACTCTTCAGGTGAAGATAGTTGATGAC
  GAGGAATATGAGAAAAAGGATAATTTCTTCATTGAGCTGGGCCAGCCCCAGTGGCTTAAGCGAGGGATT
  TCAGCTCTGCTACTCAATCAAGGGGATGGGGACAGGAAGCTAACAGCCGAGGAGGAGGAGGCTCGGAGG
  ATAGCAGAGATGGGCAAGCCAGTTCTTGGGGAGAACTGCCGGCTGGAGGTCATCATCGAGGAGTCATAT
  GATTTTAAGAACACGGTGGATAAACTCATCAAGAAAACGAACTTGGCCTTGGTAATTGGGACCCATTCA
  TGGAGGGAGCAGTTTTTAGAGGCAATTACGGTGAGCGCAGGGGACGAGGAGGAGGAGGAGGACGGGTCC
  CGGGAGGAGCGGCTGCCGTCGTGCTTTGACTACGTGATGCACTTCCTGACGGTGTTCTGGAAGGTGCTC
  TTCGCCTGTGTGCCCCCCACCGAGTACTGCCACGGCTGGGCCTGCTTTGGTGTCTCCATCCTGGTCATC
  GGCCTGCTCACCGCCCTCATTGGGGACCTCGCCTCCCACTTCGGCTGCACCGTTGGCCTCAAGGACTCT
  GTCAATGCTGTTGTCTTCGTTGCCCTGGGCACCTCCATCCCTGACACGTTCGCCAGCAAGGTGGCGGCG
  CTGCAGGACCAGTGCGCCGACGCGTCCATCGGCAACGTGACCGGCTCCAACGCGGTGAACGTGTTCCTT
  GGCCTGGGCGTCGCCTGGTCTGTGGCCGCCGTGTACTGGGCGGTGCAGGGCCGCCCCTTCGAGGTGCGC
  ACTGGCACGCTGGCCTTCTCCGTCACGCTCTTCACCGTCTTCGCCTTCGTGGGCATTGCCGTGCTGCTG
  TACCGGCGCCGGCCGCACATCGGCGGCGAGCTGGGCGGCCCGCGCGGACCCAAGCTCGCCACCACCGCG
  CTCTTCCTGGGCCTCTGGCTCCTGTACATCCTCTTCGCCAGCCTGGAGGCGTACTGCCACATCCGGGGC
  TTCTAG
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_015063
• Insert Size: 2766 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015063.2
• RefSeq Size: 5111 bp
• RefSeq ORF: 2766 bp
• Locus ID: 6543
• UniProt ID: Q9UPR5
• Cytogenetics: 19q13.32
• Protein Families: Transmembrane
• Protein Pathways: Calcium signaling pathway
• MW: 100.4 kDa
• Gene Summary: Mediates the electrogenic exchange of Ca(2+) against Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes. Contributes to cellular Ca(2+) homeostasis in excitable cells. Contributes to the rapid decrease of cytoplasmic Ca(2+) levels back to baseline after neuronal activation, and thereby contributes to modulate synaptic plasticity, learning and memory. Plays a role in regulating urinary Ca(2+) and Na(+) excretion.[UniProtKB/Swiss-Prot Function]