Villin (VIL1) (NM_007127) Human Tagged ORF Clone

CAT#: RC216340

VIL1 (Myc-DDK-tagged)-Human villin 1 (VIL1)

Product Images

Circular map for RC216340

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY VIL1 (Cat# RC216340, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VIL1(Cat# TA803236). Positive lysates LY416173 (100ug) and LC416173 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY416173) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216340 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified VIL1 protein (Cat# TP316340). The protein was produced from HEK293T cells transfected with VIL1 cDNA clone (Cat# RC216340) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Villin
• Synonyms: D2S1471; VIL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216340 representing NM_007127
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAAGCTGAGCGCCCAAGTCAAAGGCTCTCTCAACATCACCACCCCGGGGCTGCAGATATGGAGGA
  TCGAGGCCATGCAGATGGTGCCTGTTCCTTCCAGCACCTTTGGAAGCTTCTTCGATGGTGACTGCTACAT
  CATCCTGGCTATCCACAAGACAGCCAGCAGCCTGTCCTATGACATCCACTACTGGATTGGCCAGGACTCA
  TCCCTGGATGAGCAGGGGGCAGCTGCCATCTACACCACACAGATGGATGACTTCCTGAAGGGCCGGGCTG
  TGCAGCACCGCGAGGTCCAGGGCAACGAGAGCGAGGCCTTCCGAGGCTACTTCAAGCAAGGCCTTGTGAT
  CCGGAAAGGGGGCGTGGCTTCTGGCATGAAGCACGTGGAGACCAACTCCTATGACGTCCAGAGGCTGCTG
  CATGTCAAGGGCAAGAGGAACGTGGTAGCTGGAGAGGTAGAGATGTCCTGGAAGAGTTTCAACCGAGGGG
  ATGTTTTCCTCCTGGACCTTGGGAAGCTTATCATCCAGTGGAATGGACCGGAAAGCACCCGTATGGAGAG
  ACTCAGGGGCATGACTCTGGCCAAGGAGATCCGAGACCAGGAGCGGGGAGGGCGCACCTATGTAGGCGTG
  GTGGACGGAGAGAATGAATTGGCATCCCCGAAGCTGATGGAGGTGATGAACCACGTGCTGGGCAAGCGCA
  GGGAGCTGAAGGCGGCCGTGCCCGACACGGTGGTGGAGCCGGCACTCAAGGCTGCACTCAAACTGTACCA
  TGTGTCTGACTCCGAGGGGAATCTGGTGGTGAGGGAAGTCGCCACACGGCCACTGACACAGGACCTGCTC
  AGTCACGAGGACTGTTACATCCTGGACCAGGGGGGCCTGAAGATCTACGTGTGGAAAGGGAAGAAAGCCA
  ATGAGCAGGAGAAGAAGGGAGCCATGAGCCATGCGCTGAACTTCATCAAAGCCAAGCAGTACCCACCAAG
  CACACAGGTGGAGGTGCAGAATGATGGGGCTGAGTCGGCCGTCTTTCAGCAGCTCTTCCAGAAGTGGACA
  GCGTCCAACCGGACCTCAGGCCTAGGCAAAACCCACACTGTGGGCTCCGTGGCCAAAGTGGAACAGGTGA
  AGTTCGATGCCACATCCATGCATGTCAAGCCTCAGGTGGCTGCCCAGCAGAAGATGGTAGATGATGGGAG
  TGGGGAAGTGCAGGTGTGGCGCATTGAGAACCTAGAGCTGGTACCTGTGGATTCCAAGTGGCTAGGCCAC
  TTCTATGGGGGCGACTGCTACCTGCTGCTCTACACCTACCTCATCGGCGAGAAGCAGCATTACCTGCTCT
  ACGTTTGGCAGGGCAGCCAGGCCAGCCAAGATGAAATTACAGCATCAGCTTATCAAGCCGTCATCCTGGA
  CCAGAAGTACAATGGTGAACCAGTCCAGATCCGGGTCCCAATGGGCAAGGAGCCACCTCATCTTATGTCC
  ATCTTCAAGGGACGCATGGTGGTCTACCAGGGAGGCACCTCCCGAACTAACAACTTGGAGACCGGGCCCT
  CCACACGGCTGTTCCAGGTCCAGGGAACTGGCGCCAACAACACCAAGGCCTTTGAGGTCCCAGCGCGGGC
  CAATTTCCTCAATTCCAATGATGTCTTTGTCCTCAAGACCCAGTCTTGCTGCTATCTATGGTGTGGGAAG
  GGTTGTAGCGGGGACGAGCGGGAGATGGCCAAGATGGTTGCTGACACCATCTCCCGGACGGAGAAGCAAG
  TGGTGGTGGAAGGGCAGGAGCCAGCCAACTTCTGGATGGCCCTGGGTGGGAAGGCCCCCTATGCCAACAC
  CAAGAGACTACAGGAAGAAAACCTGGTCATCACCCCCCGGCTCTTTGAGTGTTCCAACAAGACTGGGCGC
  TTCCTGGCCACAGAGATCCCTGACTTCAATCAGGATGACTTGGAAGAGGATGATGTGTTCCTACTAGATG
  TCTGGGACCAGGTCTTCTTCTGGATTGGGAAACATGCCAACGAGGAGGAGAAGAAGGCCGCAGCAACCAC
  TGCACAGGAATACCTCAAGACCCATCCCAGCGGGCGTGACCCTGAGACCCCCATCATTGTGGTGAAGCAG
  GGACACGAGCCCCCCACCTTCACAGGCTGGTTCCTGGCTTGGGATCCCTTCAAGTGGAGTAACACCAAAT
  CCTATGAGGACCTGAAGGCGGAGCTTGGCAACTCTAGGGACTGGAGCCAGATCACTGCTGAGGTCACAAG
  CCCCAAAGTGGACGTGTTCAATGCTAACAGCAACCTCAGTTCTGGGCCTCTGCCCATCTTCCCCCTGGAG
  CAGCTAGTGAACAAGCCTGTAGAGGAGCTCCCCGAGGGTGTGGACCCCAGCAGGAAGGAGGAACACCTGT
  CCATTGAAGATTTCACTCAGGCCTTTGGGATGACTCCAGCTGCCTTCTCTGCTCTGCCTCGATGGAAGCA
  ACAAAACCTCAAGAAAGAAAAAGGACTATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216340 representing NM_007127
  Red=Cloning site Green=Tags(s)
  
  MTKLSAQVKGSLNITTPGLQIWRIEAMQMVPVPSSTFGSFFDGDCYIILAIHKTASSLSYDIHYWIGQDS
  SLDEQGAAAIYTTQMDDFLKGRAVQHREVQGNESEAFRGYFKQGLVIRKGGVASGMKHVETNSYDVQRLL
  HVKGKRNVVAGEVEMSWKSFNRGDVFLLDLGKLIIQWNGPESTRMERLRGMTLAKEIRDQERGGRTYVGV
  VDGENELASPKLMEVMNHVLGKRRELKAAVPDTVVEPALKAALKLYHVSDSEGNLVVREVATRPLTQDLL
  SHEDCYILDQGGLKIYVWKGKKANEQEKKGAMSHALNFIKAKQYPPSTQVEVQNDGAESAVFQQLFQKWT
  ASNRTSGLGKTHTVGSVAKVEQVKFDATSMHVKPQVAAQQKMVDDGSGEVQVWRIENLELVPVDSKWLGH
  FYGGDCYLLLYTYLIGEKQHYLLYVWQGSQASQDEITASAYQAVILDQKYNGEPVQIRVPMGKEPPHLMS
  IFKGRMVVYQGGTSRTNNLETGPSTRLFQVQGTGANNTKAFEVPARANFLNSNDVFVLKTQSCCYLWCGK
  GCSGDEREMAKMVADTISRTEKQVVVEGQEPANFWMALGGKAPYANTKRLQEENLVITPRLFECSNKTGR
  FLATEIPDFNQDDLEEDDVFLLDVWDQVFFWIGKHANEEEKKAAATTAQEYLKTHPSGRDPETPIIVVKQ
  GHEPPTFTGWFLAWDPFKWSNTKSYEDLKAELGNSRDWSQITAEVTSPKVDVFNANSNLSSGPLPIFPLE
  QLVNKPVEELPEGVDPSRKEEHLSIEDFTQAFGMTPAAFSALPRWKQQNLKKEKGLF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007127
• ORF Size: 2481 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007127.3
• RefSeq Size: 2702 bp
• RefSeq ORF: 2484 bp
• Locus ID: 7429
• UniProt ID: P09327
• Cytogenetics: 2q35
• MW: 92.5 kDa
• Gene Summary: This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008]