FCRL4 (NM_031282) Human Tagged ORF Clone

CAT#: RC216335

FCRL4 (Myc-DDK-tagged)-Human Fc receptor-like 4 (FCRL4)

Product Images

Circular map for RC216335

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  Western blot validation of overexpression lysate (Cat# LY410574) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216335 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified FCRL4 protein (Cat# TP316335). The protein was produced from HEK293T cells transfected with FCRL4 cDNA clone (Cat# RC216335) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FCRL4
• Synonyms: CD307d; FCRH4; IGFP2; IRTA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216335 representing NM_031282
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCTGTGGGCGTCCTTGCTGGCCTTTGCTCCAGTCTGTGGACAATCTGCAGCTGCACACAAACCTG
  TGATTTCCGTCCATCCTCCATGGACCACATTCTTCAAAGGAGAGAGAGTGACTCTGACTTGCAATGGATT
  TCAGTTCTATGCAACAGAGAAAACAACATGGTATCATCAGCACTACTGGGGAGAAAAGTTGACCCTGACC
  CCAGGAAACACCCTCGAGGTTCGGGAATCTGGACTGTACAGATGCCAGGCCCGGGGCTCCCCACGAAGTA
  ACCCTGTGCGCTTGCTCTTTTCTTCAGACTCCTTAATCCTGCAGGCACCATATTCTGTGTTTGAAGGTGA
  CACATTGGTTCTGAGATGCCACAGAAGAAGGAAAGAGAAATTGACTGCTGTGAAATATACTTGGAATGGA
  AACATTCTTTCCATTTCTAATAAAAGCTGGGATCTTCTTATCCCACAAGCAAGTTCAAATAACAATGGCA
  ATTATCGATGCATTGGATATGGAGATGAGAATGATGTATTTAGATCAAATTTCAAAATAATTAAAATTCA
  AGAACTATTTCCACATCCAGAGCTGAAAGCTACAGACTCTCAGCCTACAGAGGGGAATTCTGTAAACCTG
  AGCTGTGAAACACAGCTTCCTCCAGAGCGGTCAGACACCCCACTTCACTTCAACTTCTTCAGAGATGGCG
  AGGTCATCCTGTCAGACTGGAGCACGTACCCGGAACTCCAGCTCCCAACCGTCTGGAGAGAAAACTCAGG
  ATCCTATTGGTGTGGTGCTGAAACAGTGAGGGGTAACATCCACAAGCACAGTCCCTCGCTACAGATCCAT
  GTGCAGCGGATCCCTGTGTCTGGGGTGCTCCTGGAGACCCAGCCCTCAGGGGGCCAGGCTGTTGAAGGGG
  AGATGCTGGTCCTTGTCTGCTCCGTGGCTGAAGGCACAGGGGATACCACATTCTCCTGGCACCGAGAGGA
  CATGCAGGAGAGTCTGGGGAGGAAAACTCAGCGTTCCCTGAGAGCAGAGCTGGAGCTCCCTGCCATCAGA
  CAGAGCCATGCAGGGGGATACTACTGTACAGCAGACAACAGCTACGGCCCTGTCCAGAGCATGGTGCTGA
  ATGTCACTGTGAGAGAGACCCCAGGCAACAGAGATGGCCTTGTCGCCGCGGGAGCCACTGGAGGGCTGCT
  CAGTGCTCTTCTCCTGGCTGTGGCCCTGCTGTTTCACTGCTGGCGTCGGAGGAAGTCAGGAGTTGGTTTC
  TTGGGAGACGAAACCAGGCTCCCTCCCGCTCCAGGCCCAGGAGAGTCCTCCCATTCCATCTGCCCTGCCC
  AGGTGGAGCTTCAGTCGTTGTATGTTGATGTACACCCCAAAAAGGGAGATTTGGTATACTCTGAGATCCA
  GACTACTCAGCTGGGAGAAGAAGAGGAAGCTAATACCTCCAGGACACTTCTAGAGGATAAGGATGTCTCA
  GTTGTCTACTCTGAGGTAAAGACACAACACCCAGATAACTCAGCTGGAAAGATCAGCTCTAAGGATGAAG
  AAAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216335 representing NM_031282
  Red=Cloning site Green=Tags(s)
  
  MLLWASLLAFAPVCGQSAAAHKPVISVHPPWTTFFKGERVTLTCNGFQFYATEKTTWYHQHYWGEKLTLT
  PGNTLEVRESGLYRCQARGSPRSNPVRLLFSSDSLILQAPYSVFEGDTLVLRCHRRRKEKLTAVKYTWNG
  NILSISNKSWDLLIPQASSNNNGNYRCIGYGDENDVFRSNFKIIKIQELFPHPELKATDSQPTEGNSVNL
  SCETQLPPERSDTPLHFNFFRDGEVILSDWSTYPELQLPTVWRENSGSYWCGAETVRGNIHKHSPSLQIH
  VQRIPVSGVLLETQPSGGQAVEGEMLVLVCSVAEGTGDTTFSWHREDMQESLGRKTQRSLRAELELPAIR
  QSHAGGYYCTADNSYGPVQSMVLNVTVRETPGNRDGLVAAGATGGLLSALLLAVALLFHCWRRRKSGVGF
  LGDETRLPPAPGPGESSHSICPAQVELQSLYVDVHPKKGDLVYSEIQTTQLGEEEEANTSRTLLEDKDVS
  VVYSEVKTQHPDNSAGKISSKDEES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031282
• ORF Size: 1545 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031282.3
• RefSeq Size: 3459 bp
• RefSeq ORF: 1548 bp
• Locus ID: 83417
• UniProt ID: Q96PJ5
• Cytogenetics: 1q23.1
• Protein Families: Transmembrane
• MW: 55.2 kDa
• Gene Summary: This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein has four extracellular C2-type immunoglobulin domains, a transmembrane domain and a cytoplasmic domain that contains three immune-receptor tyrosine-based inhibitory motifs. This protein may play a role in the function of memory B-cells in the epithelia. Aberrations in the chromosomal region encoding this gene are associated with non-Hodgkin lymphoma and multiple myeloma. [provided by RefSeq, Apr 2009]