NHLRC2 (NM_198514) Human Tagged ORF Clone

CAT#: RC216229

NHLRC2 (Myc-DDK-tagged)-Human NHL repeat containing 2 (NHLRC2)

Product Images

Circular map for RC216229

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  Western blot validation of overexpression lysate (Cat# LY404900) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216229 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NHLRC2 protein (Cat# TP316229). The protein was produced from HEK293T cells transfected with NHLRC2 cDNA clone (Cat# RC216229) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NHLRC2
• Synonyms: FINCA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216229 representing NM_198514
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCCCGGAGGCCGGGGCCGCAGCCTCTCCGGCCTGCTCCCCGCGCAGACCTCGCTAGAGTACG
  CCCTGCTCGACGCCGTTACCCAGCAGGAGAAGGACAGCCTGGTCTACCAGTATCTGCAGAAGGTGGACGG
  CTGGGAGCAGGACTTGTCAGTACCCGAGTTTCCGGAAGGATTAGAATGGCTGAACACAGAAGAACCTATT
  TCTGTCTACAAGGATCTATGTGGAAAAATAGTCGTCCTTGATTTCTTCACCTACTGCTGCATAAACTGTA
  TTCACCTATTGCCTGATCTCCATGCATTAGAACACACATACTCTGATAAAGATGGTCTTCTTATTATTGG
  TGTTCACTCGGCTAAGTTTCCAAATGAAAAAGTCCTGGATAACATTAAGAGTGCTGTTCTTCGATACAAC
  ATCACCCACCCTATGGTTAATGATGCAGATGCCAGCCTTTGGCAAGAACTAGAAGTTTCCTGCTGGCCAA
  CTCTAGTCATACTTGGACCTCGTGGAAACATGTTGTTTTCTTTGATTGGAGAGGGACACAAAGATAAATT
  ATTTTTATATACTTCAATTGCTTTAAAGTATTACAAAGACAGGGGGCAGATCAGAGATAATAAAATTGGA
  ATAAAACTCTATAAAGATTCTTTGCCACCTTCACCATTGCTATTTCCTGGCAAAGTAACAGTAGACCAAG
  TTACTGATAGATTGGTAATAGCAGACACTGGACATCATAGAATTTTGGTCGTTTGGAAGAATGGACAAAT
  TCAATATAGCATTGGAGGACCCAACCCTGGAAGAAAAGATGGAATATTTTCAGAATCAACTTTTAATTCT
  CCACAGGGTGTAGCCATAATGAATAATATCATATATGTGGCAGACACTGAAAACCACCTTATAAGAAAGA
  TTGACCTAGAAGCTGAGAAGGTGAGCACTGTAGCTGGTATTGGAATTCAAGGTACAGATAAAGAAGGTGG
  AGCAAAAGGAGAACAACAACCCATTAGTTCCCCTTGGGATGTAGTTTTTGGAACATCAGGTTCAGAGGTC
  CAAAGAGGTGACATTTTATGGATAGCCATGGCAGGGACTCATCAGATATGGGCACTCCTGCTGGACTCTG
  GCAAACTGCCAAAGAAAAATGAGTTAACAAAAGGAACCTGCCTTAGGTTTGCTGGAAGTGGAAATGAAGA
  GAATCGAAACAATGCCTATCCTCACAAGGCAGGTTTTGCCCAACCTTCAGGCCTTTCCTTGGCCTCTGAA
  GATCCCTGGAGCTGCTTGTTTGTAGCAGATAGTGAGAGCAGTACAGTGAGAACCGTTTCACTGAAAGATG
  GAGCAGTGAAGCACCTCGTAGGAGGAGAAAGAGACCCCATGAATTTATTTGCTTTTGGTGATGTTGATGG
  AGTAGGAATCAATGCAAAGCTTCAACACCCCCTTGGAGTAACATGGGACAAAAAAAGGAATTTACTTTAT
  GTTGCAGACTCCTACAATCACAAGATTAAAGTTGTGGATCCAAAAACAAAAAACTGTACAACATTAGCAG
  GAACTGGAGACACAAATAATGTTACCAGTTCCAGTTTTACAGAGTCAACTTTTAATGAACCAGGAGGCTT
  GTGTATTGGAGAGAATGGAGAATTATTATATGTAGCAGACACCAATAATCATCAAATTAAAGTGATGGAT
  TTAGAAACTAAAATGGTATCTGTGCTCCCCATCTTCAGATCTGAAAATGCTGTGGTAGATGGCCCGTTCC
  TAGTAGAAAAACAGAAGACATTACCCAAACTACCTAAATCTGCTCCAAGCATTAGGCTTTCCCCCGTGAC
  TGCGTGTGCTGGCCAGACTCTTCAGTTCAAACTCAGATTAGACCTCCCATCAGGATCAAAGCTAACTGAA
  GGAGTATCCAGTTGCTGGTTTCTAACAGCTGAAGGCAATGAATGGCTACTTCAAGGACAGATAGCAGCTG
  GAGATATAGAGAACATTTCCAGTCAACCAACAATTTCACTACAAATTCCTGATGATTGCTTATCACTTGA
  AGCCATTGTATCTGTCAGTGTGTTTCTTTATTACTGTAGTGCAGACAGCAGTGCTTGTATGATGAAGGCA
  ATTTTGTTCAGTCAGCCTTTACAAATAACGGATACACAGCAAGGTTGCATAGCTCCAGTAGAGCTCAGGT
  ATGTATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216229 representing NM_198514
  Red=Cloning site Green=Tags(s)
  
  MAAPGGRGRSLSGLLPAQTSLEYALLDAVTQQEKDSLVYQYLQKVDGWEQDLSVPEFPEGLEWLNTEEPI
  SVYKDLCGKIVVLDFFTYCCINCIHLLPDLHALEHTYSDKDGLLIIGVHSAKFPNEKVLDNIKSAVLRYN
  ITHPMVNDADASLWQELEVSCWPTLVILGPRGNMLFSLIGEGHKDKLFLYTSIALKYYKDRGQIRDNKIG
  IKLYKDSLPPSPLLFPGKVTVDQVTDRLVIADTGHHRILVVWKNGQIQYSIGGPNPGRKDGIFSESTFNS
  PQGVAIMNNIIYVADTENHLIRKIDLEAEKVSTVAGIGIQGTDKEGGAKGEQQPISSPWDVVFGTSGSEV
  QRGDILWIAMAGTHQIWALLLDSGKLPKKNELTKGTCLRFAGSGNEENRNNAYPHKAGFAQPSGLSLASE
  DPWSCLFVADSESSTVRTVSLKDGAVKHLVGGERDPMNLFAFGDVDGVGINAKLQHPLGVTWDKKRNLLY
  VADSYNHKIKVVDPKTKNCTTLAGTGDTNNVTSSSFTESTFNEPGGLCIGENGELLYVADTNNHQIKVMD
  LETKMVSVLPIFRSENAVVDGPFLVEKQKTLPKLPKSAPSIRLSPVTACAGQTLQFKLRLDLPSGSKLTE
  GVSSCWFLTAEGNEWLLQGQIAAGDIENISSQPTISLQIPDDCLSLEAIVSVSVFLYYCSADSSACMMKA
  ILFSQPLQITDTQQGCIAPVELRYVF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198514
• ORF Size: 2178 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198514.4
• RefSeq Size: 2550 bp
• RefSeq ORF: 2181 bp
• Locus ID: 374354
• UniProt ID: Q8NBF2
• Cytogenetics: 10q25.3
• Protein Families: Druggable Genome
• MW: 79.3 kDa
• Gene Summary: Required for normal embryonic development.[UniProtKB/Swiss-Prot Function]