beta 1 Spectrin (SPTB) (NM_001024858) Human Tagged ORF Clone

CAT#: RC216157

SPTB (Myc-DDK-tagged)-Human spectrin, beta, erythrocytic (SPTB), transcript variant 1

Product Images

Circular map for RC216157

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: beta 1 Spectrin
• Synonyms: EL3; HS2; HSPTB1; SPH2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216157 representing NM_001024858
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACATCGGCCACAGAGTTTGAAAATGTGGGCAACCAGCCACCTTACAGCAGGATCAATGCCCGCTGGG
  ACGCCCCAGACGACGAGCTGGATAATGACAACAGCTCAGCCAGGCTCTTTGAGAGGTCCCGGATAAAGGC
  CTTGGCAGATGAGCGGGAAGTTGTTCAGAAAAAGACCTTCACGAAATGGGTGAACTCGCACCTGGCTCGA
  GTGTCCTGCCGCATCACCGATCTCTACAAGGACCTGCGGGATGGGCGCATGCTCATCAAGCTGCTGGAGG
  TGCTCTCTGGAGAGATGCTGCCAAAGCCCACCAAGGGGAAGATGCGCATCCACTGCCTGGAGAATGTGGA
  CAAGGCTCTCCAGTTCCTCAAGGAGCAGCGTGTACACCTGGAGAACATGGGCTCCCACGACATTGTAGAT
  GGCAACCACCGCCTGGTCCTGGGCCTCATCTGGACCATCATCCTCCGCTTCCAGATTCAGGACATTGTGG
  TCCAAACTCAGGAAGGTCGTGAAACACGCTCAGCCAAGGATGCGTTGCTGTTGTGGTGTCAGATGAAGAC
  GGCAGGCTACCCTCATGTTAATGTCACCAACTTTACCTCCAGCTGGAAGGATGGCTTGGCCTTTAATGCC
  CTGATACACAAGCACCGGCCCGACCTGATCGACTTTGATAAGCTGAAGGACTCCAATGCCCGGCACAACC
  TGGAGCACGCATTCAATGTGGCTGAGCGCCAGCTGGGCATCATCCCGCTCCTCGACCCCGAAGATGTCTT
  TACGGAAAACCCTGATGAGAAATCCATCATCACCTATGTGGTGGCCTTTTACCACTACTTCTCCAAGATG
  AAGGTGCTGGCAGTGGAGGGCAAGCGTGTCGGCAAGGTTATTGACCATGCCATTGAGACTGAGAAGATGA
  TTGAAAAGTACAGCGGGCTAGCCTCGGACCTGCTCACCTGGATCGAGCAGACCATCACTGTCCTGAACAG
  CCGCAAGTTTGCCAACTCGCTGACGGGCGTCCAGCAGCAGCTGCAGGCCTTCAGCACCTACCGCACCGTG
  GAGAAGCCGCCCAAGTTTCAAGAGAAGGGGAATCTGGAAGTTCTACTTTTTACCATCCAGTCCCGGATGA
  GAGCCAACAATCAGAAAGTGTACACACCCCACGATGGGAAACTAGTGTCTGACATCAACAGGGCCTGGGA
  AAGCCTGGAGGAAGCTGAGTATCGGCGGGAGCTGGCCCTGAGAAATGAGCTCATTCGGCAGGAGAAGCTA
  GAGCAACTGGCCCGGCGCTTTGACCGGAAGGCCGCAATGAGAGAGACCTGGCTCAGTGAAAACCAGCGCC
  TCGTGGCCCAGGATAACTTTGGGTATGACCTGGCAGCTGTGGAGGCCGCCAAGAAGAAGCATGAGGCCAT
  CGAGACCGACACGGCTGCCTACGAGGAGCGGGTGAGAGCCCTGGAGGACCTGGCTCAGGAGCTGGAGAAA
  GAGAACTACCATGACCAGAAGCGCATCACGGCCCGCAAGGACAATATACTGCGCCTATGGAGCTACCTGC
  AGGAGCTGCTGCAGTCCCGGCGCCAGAGGCTCGAGACCACCCTGGCACTGCAGAAGCTCTTCCAGGACAT
  GCTGCACAGCATCGACTGGATGGATGAGATCAAGGCTCACCTCTTGTCTGCCGAGTTTGGGAAGCACTTG
  TTGGAGGTTGAAGACCTGCTACAGAAGCACAAGTTGATGGAAGCTGACATCGCCATCCAAGGGGACAAAG
  TGAAGGCCATCACCGCAGCCACCCTGAAGTTCACCGAGGGGAAAGGGTACCAGCCTTGTGACCCCCAGGT
  CATCCAGGACCGCATCAGCCACTTGGAGCAGTGCTTTGAGGAGCTGAGCAACATGGCAGCTGGGCGGAAG
  GCCCAACTGGAGCAGTCCAAACGACTCTGGAAGTTCTTCTGGGAGATGGATGAGGCTGAGAGCTGGATCA
  AGGAGAAGGAGCAGATCTATTCTTCCCTGGACTATGGCAAAGACCTGACCAGTGTGCTCATCTTACAGCG
  CAAGCACAAGGCCTTTGAGGATGAGCTCCGTGGGCTGGATGCTCACCTGGAGCAGATCTTCCAGGAGGCT
  CATGGCATGGTTGCGCGCAAGCAGTTTGGGCACCCGCAGATCGAGGCCCGCATAAAGGAGGTGTCGGCAC
  AGTGGGACCAGCTGAAGGACCTGGCTGCCTTCTGCAAGAAGAACCTCCAGGATGCTGAGAACTTTTTCCA
  GTTCCAGGGCGATGCGGATGACCTGAAGGCTTGGCTGCAAGACGCCCACCGGCTGCTCTCTGGTGAAGAT
  GTGGGGCAGGACGAAGGGGCCACGCGGGCCCTGGGGAAAAAGCACAAGGACTTCCTGGAGGAGCTGGAGG
  AGAGCCGTGGGGTGATGGAGCACCTGGAGCAGCAGGCCCAGGGATTCCCCGAAGAGTTTCGGGATTCCCC
  AGATGTGACCCATCGGCTGCAGGCCCTGCGGGAGCTCTACCAACAGGTGGTGGCCCAGGCGGACCTGCGT
  CAGCAGAGGCTGCAGGAAGCCCTGGACCTGTACACGGTGTTCGGGGAGACAGACGCCTGTGAGCTGTGGA
  TGGGAGAGAAGGAGAAGTGGCTGGCCGAGATGGAAATGCCAGACACCCTGGAGGACCTGGAGGTCGTGCA
  GCACAGGTTCGACATCCTGGACCAGGAGATGAAGACCTTGATGACTCAGATTGATGGTGTGAACCTCGCT
  GCCAACAGCTTGGTAGAGAGTGGCCACCCACGCAGCAGGGAGGTGAAGCAGTACCAGGACCATCTGAACA
  CCAGGTGGCAGGCATTTCAGACCCTGGTGTCGGAGCGGCGGGAGGCTGTGGACTCAGCCCTCCGAGTGCA
  CAACTACTGCGTAGATTGCGAGGAGACCAGCAAGTGGATCACGGACAAGACAAAGGTAGTGGAGTCCACA
  AAAGACCTGGGGCGGGACCTGGCAGGTATCATCGCCATCCAGAGGAAGTTGTCAGGGCTGGAGCGTGACG
  TGGCCGCCATCCAGGCCCGTGTGGATGCCCTGGAGCGTGAGTCCCAGCAGCTGATGGACTCGCACCCTGA
  GCAGAAGGAGGATATTGGTCAGCGGCAAAAACACTTGGAGGAGCTGTGGCAGGGCCTGCAGCAATCCCTG
  CAGGGCCAGGAGGACTTGCTGGGGGAAGTCAGCCAGCTGCAGGCCTTCCTGCAGGATCTGGATGACTTCC
  AGGCCTGGCTCTCCATCACCCAGAAGGCTGTGGCCTCTGAGGACATGCCCGAATCCCTCCCAGAGGCTGA
  GCAGCTCCTGCAGCAGCATGCAGGTATCAAGGATGAGATTGACGGGCACCAAGACAGCTACCAGCGTGTT
  AAGGAGTCTGGGGAGAAAGTGATCCAAGGCCAGACGGACCCAGAGTATCTGCTTCTGGGCCAGCGGCTGG
  AGGGCCTGGATACTGGCTGGAATGCCCTGGGCAGGATGTGGGAGAGCCGCAGCCACACCCTCGCTCAGTG
  CCTTGGCTTCCAGGAGTTCCAGAAAGATGCCAAGCAGGCTGAAGCCATCCTCAGCAACCAGGAATACACT
  CTGGCTCACTTGGAGCCCCCAGACTCCCTGGAAGCTGCAGAGGCTGGGATCCGGAAGTTTGAGGATTTCT
  TGGGGTCTATGGAGAACAACCGGGATAAGGTCTTGAGTCCTGTGGACTCTGGAAACAAGCTGGTAGCTGA
  GGGAAACCTATACTCAGACAAGATCAAGGAGAAGGTGCAGCTGATTGAGGACAGGCACAGGAAGAACAAC
  GAGAAGGCCCAGGAGGCCTCTGTCCTACTGAGAGACAACCTGGAGCTACAGAACTTCCTCCAGAACTGCC
  AGGAGCTCACTCTCTGGATCAACGACAAGCTGCTGACATCTCAGGATGTCTCCTATGATGAAGCACGAAA
  CCTTCACAATAAATGGCTAAAGCACCAGGCGTTTGTGGCAGAGCTGGCTTCCCATGAAGGGTGGCTAGAG
  AACATCGATGCGGAAGGAAAGCAGCTGATGGATGAGAAGCCCCAGTTTACAGCCCTGGTGTCCCAAAAGC
  TGGAAGCCCTGCACCGGCTCTGGGACGAGCTGCAGGCCACCACAAAGGAGAAGACCCAGCACCTCTCGGC
  TGCCAGGAGCTCCGACCTGCGCTTGCAGACCCATGCTGACCTCAACAAGTGGATCAGCGCCATGGAGGAC
  CAGCTGCGGTCAGACGACCCGGGCAAGGACCTGACCAGTGTCAATCGGATGTTGGCTAAGCTGAAGCGAG
  TGGAGGACCAAGTGAATGTGCGAAAAGAGGAGCTGGGGGAGCTGTTTGCCCAGGTGCCTTCAATGGGAGA
  GGAGGGAGGAGATGCAGACTTGAGCATCGAGAAGCGGTTCCTGGACCTCCTGGAACCCCTAGGAAGGAGG
  AAGAAGCAGCTGGAATCATCCAGAGCCAAGCTGCAGATCAGCCGGGACTTAGAGGATGAGACGCTTTGGG
  TGGAGGAGAGGCTGCCTCTGGCCCAGTCAGCCGACTATGGCACTAATCTGCAAACTGTGCAACTGTTCAT
  GAAGAAGAACCAGACACTGCAGAATGAGATTCTGGGCCATACGCCGCGGGTTGAGGATGTGCTGCAGAGA
  GGGCAGCAGCTGGTGGAGGCGGCGGAGATCGACTGCCAGGACCTTGAGGAGCGCCTGGGGCACCTGCAGA
  GCTCCTGGGACAGGCTGCGGGAGGCAGCGGCCGGGAGGCTGCAGCGACTGAGGGACGCCAACGAGGCACA
  GCAGTACTACCTGGATGCAGACGAGGCTGAGGCCTGGATTGGCGAGCAGGAGCTCTACGTCATCTCCGAT
  GAGATCCCCAAGGATGAAGAGGGCGCCATTGTGATGCTGAAGCGACATTTGCGGCAGCAGCGTGCGGTGG
  AGGACTACGGCCGGAACATCAAGCAGCTGGCCAGCCGGGCCCAGGGCCTGCTGTCTGCAGGCCACCCTGA
  GGGGGAACAGATCATCAGACTTCAGGGGCAAGTGGACAAGCACTACGCAGGGCTGAAGGACGTGGCGGAA
  GAGCGCAAGCGCAAGCTGGAGAACATGTACCACCTGTTCCAGCTCAAGCGGGAGACCGACGACCTGGAGC
  AGTGGATTTCAGAAAAGGAGCTAGTGGCCTCTTCCCCGGAAATGGGGCAAGACTTTGACCACGTGACTCT
  TCTGCGGGACAAGTTCCGGGACTTTGCCCGGGAGACCGGGGCGATTGGGCAGGAGCGGGTGGACAATGTG
  AATGCCTTCATCGAGCGACTCATCGACGCGGGCCACAGCGAGGCGGCCACCATCGCCGAGTGGAAGGACG
  GGCTGAACGAGATGTGGGCAGACCTCCTGGAGCTCATTGACACGCGCATGCAGCTGCTGGCCGCCTCCTA
  TGACCTGCACCGCTACTTCTACACGGGTGCCGAGATCCTGGGCCTCATCGACGAGAAGCACCGCGAGCTG
  CCCGAGGACGTGGGGCTGGACGCCAGCACGGCCGAGTCCTTCCACCGGGTGCACACAGCCTTCGAGCGGG
  AGCTCCACCTGCTGGGTGTCCAGGTGCAGCAGTTCCAGGACGTGGCCACCCGTCTGCAGACAGCATATGC
  TGGGGAGAAGGCAGAGGCCATCCAGAACAAGGAGCAGGAGGTGTCTGCCGCGTGGCAGGCGCTGCTCGAT
  GCCTGTGCCGGGCGCCGGACCCAGCTAGTGGACACGGCGGATAAATTCCGCTTCTTCAGCATGGCCCGTG
  ACCTCCTCTCCTGGATGGAGAGCATCATCCGGCAGATCGAGACCCAGGAGAGGCCCAGGGATGTCTCCTC
  TGTGGAACTGCTCATGAAGTATCACCAGGGCATCAATGCAGAGATTGAAACCCGGAGCAAGAACTTCAGT
  GCCTGCCTGGAGCTTGGCGAGTCCCTGCTGCAGCGGCAGCACCAGGCCTCAGAGGAGATCCGCGAGAAAC
  TGCAGCAGGTGATGTCCAGGAGGAAAGAGATGAATGAGAAGTGGGAAGCCCGCTGGGAGCGGCTCCGCAT
  GTTGCTGGAGGTGTGCCAGTTCTCGAGGGATGCCTCTGTGGCTGAGGCGTGGCTGATTGCCCAGGAGCCC
  TACCTGGCCAGCGGGGACTTTGGACACACAGTGGACAGTGTGGAGAAGCTCATCAAGAGGCATGAGGCTT
  TTGAGAAGTCCACGGCCAGCTGGGCAGAGCGCTTTGCTGCCCTGGAGAAGCCCACCACGCTTGAGCTGAA
  AGAACGCCAGATTGCAGAGAGACCCGCAGAGGAGACTGGGCCTCAAGAGGAGGAAGGCGAGACAGCAGGG
  GAGGCTCCAGTTTCCCACCATGCGGCCACCGAGAGAACGTCCCCGGGGGAAGAAGAGGGAACGTGGCCTC
  AGAACCTGCAGCAGCCACCACCGCCGGGTCAGCACAAGGATGGGCAGAAATCCACTGGGGATGAGAGGCC
  CACCACGGAGCCCCTCTTTAAGGTCCTAGATACGCCTCTGAGCGAGGGTGATGAGCCCGCAACGCTGCCG
  GCCCCGCGGGACCATGGGCAGAGTGTGCAGATGGAAGGCTACCTGGGCCGCAAGCATGACCTGGAGGGGC
  CCAACAAGAAGGCTTCCAACAGGTCCTGGAACAACCTGTACTGTGTGCTCAGGAACAGTGAGCTAACCTT
  CTACAAGGATGCCAAGAACCTGGCCCTGGGGATGCCCTACCATGGGGAGGAACCCCTGGCCCTGAGACAT
  GCCATCTGTGAGATTGCTGCCAACTACAAGAAGAAGAAGCACGTCTTTAAGCTGAGGCTGAGTAATGGCA
  GCGAGTGGCTCTTCCATGGCAAGGATGAGGAGGAGATGCTGTCCTGGCTGCAGGGCGTGAGCACCGCCAT
  CAACGAGTCCCAGAGCATCCGCGTCAAGGCGCAGAGCCTGCCCCTGCCTTCCCTCTCCGGCCCCGACGCC
  AGCCTCGGCAAGAAAGACAAGGAGAAGAGATTCAGCTTCTTCCCCAAAAAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216157 representing NM_001024858
  Red=Cloning site Green=Tags(s)
  
  MTSATEFENVGNQPPYSRINARWDAPDDELDNDNSSARLFERSRIKALADEREVVQKKTFTKWVNSHLAR
  VSCRITDLYKDLRDGRMLIKLLEVLSGEMLPKPTKGKMRIHCLENVDKALQFLKEQRVHLENMGSHDIVD
  GNHRLVLGLIWTIILRFQIQDIVVQTQEGRETRSAKDALLLWCQMKTAGYPHVNVTNFTSSWKDGLAFNA
  LIHKHRPDLIDFDKLKDSNARHNLEHAFNVAERQLGIIPLLDPEDVFTENPDEKSIITYVVAFYHYFSKM
  KVLAVEGKRVGKVIDHAIETEKMIEKYSGLASDLLTWIEQTITVLNSRKFANSLTGVQQQLQAFSTYRTV
  EKPPKFQEKGNLEVLLFTIQSRMRANNQKVYTPHDGKLVSDINRAWESLEEAEYRRELALRNELIRQEKL
  EQLARRFDRKAAMRETWLSENQRLVAQDNFGYDLAAVEAAKKKHEAIETDTAAYEERVRALEDLAQELEK
  ENYHDQKRITARKDNILRLWSYLQELLQSRRQRLETTLALQKLFQDMLHSIDWMDEIKAHLLSAEFGKHL
  LEVEDLLQKHKLMEADIAIQGDKVKAITAATLKFTEGKGYQPCDPQVIQDRISHLEQCFEELSNMAAGRK
  AQLEQSKRLWKFFWEMDEAESWIKEKEQIYSSLDYGKDLTSVLILQRKHKAFEDELRGLDAHLEQIFQEA
  HGMVARKQFGHPQIEARIKEVSAQWDQLKDLAAFCKKNLQDAENFFQFQGDADDLKAWLQDAHRLLSGED
  VGQDEGATRALGKKHKDFLEELEESRGVMEHLEQQAQGFPEEFRDSPDVTHRLQALRELYQQVVAQADLR
  QQRLQEALDLYTVFGETDACELWMGEKEKWLAEMEMPDTLEDLEVVQHRFDILDQEMKTLMTQIDGVNLA
  ANSLVESGHPRSREVKQYQDHLNTRWQAFQTLVSERREAVDSALRVHNYCVDCEETSKWITDKTKVVEST
  KDLGRDLAGIIAIQRKLSGLERDVAAIQARVDALERESQQLMDSHPEQKEDIGQRQKHLEELWQGLQQSL
  QGQEDLLGEVSQLQAFLQDLDDFQAWLSITQKAVASEDMPESLPEAEQLLQQHAGIKDEIDGHQDSYQRV
  KESGEKVIQGQTDPEYLLLGQRLEGLDTGWNALGRMWESRSHTLAQCLGFQEFQKDAKQAEAILSNQEYT
  LAHLEPPDSLEAAEAGIRKFEDFLGSMENNRDKVLSPVDSGNKLVAEGNLYSDKIKEKVQLIEDRHRKNN
  EKAQEASVLLRDNLELQNFLQNCQELTLWINDKLLTSQDVSYDEARNLHNKWLKHQAFVAELASHEGWLE
  NIDAEGKQLMDEKPQFTALVSQKLEALHRLWDELQATTKEKTQHLSAARSSDLRLQTHADLNKWISAMED
  QLRSDDPGKDLTSVNRMLAKLKRVEDQVNVRKEELGELFAQVPSMGEEGGDADLSIEKRFLDLLEPLGRR
  KKQLESSRAKLQISRDLEDETLWVEERLPLAQSADYGTNLQTVQLFMKKNQTLQNEILGHTPRVEDVLQR
  GQQLVEAAEIDCQDLEERLGHLQSSWDRLREAAAGRLQRLRDANEAQQYYLDADEAEAWIGEQELYVISD
  EIPKDEEGAIVMLKRHLRQQRAVEDYGRNIKQLASRAQGLLSAGHPEGEQIIRLQGQVDKHYAGLKDVAE
  ERKRKLENMYHLFQLKRETDDLEQWISEKELVASSPEMGQDFDHVTLLRDKFRDFARETGAIGQERVDNV
  NAFIERLIDAGHSEAATIAEWKDGLNEMWADLLELIDTRMQLLAASYDLHRYFYTGAEILGLIDEKHREL
  PEDVGLDASTAESFHRVHTAFERELHLLGVQVQQFQDVATRLQTAYAGEKAEAIQNKEQEVSAAWQALLD
  ACAGRRTQLVDTADKFRFFSMARDLLSWMESIIRQIETQERPRDVSSVELLMKYHQGINAEIETRSKNFS
  ACLELGESLLQRQHQASEEIREKLQQVMSRRKEMNEKWEARWERLRMLLEVCQFSRDASVAEAWLIAQEP
  YLASGDFGHTVDSVEKLIKRHEAFEKSTASWAERFAALEKPTTLELKERQIAERPAEETGPQEEEGETAG
  EAPVSHHAATERTSPGEEEGTWPQNLQQPPPPGQHKDGQKSTGDERPTTEPLFKVLDTPLSEGDEPATLP
  APRDHGQSVQMEGYLGRKHDLEGPNKKASNRSWNNLYCVLRNSELTFYKDAKNLALGMPYHGEEPLALRH
  AICEIAANYKKKKHVFKLRLSNGSEWLFHGKDEEEMLSWLQGVSTAINESQSIRVKAQSLPLPSLSGPDA
  SLGKKDKEKRFSFFPKKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024858
• ORF Size: 6984 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024858.3
• RefSeq Size: 10064 bp
• RefSeq ORF: 6987 bp
• Locus ID: 6710
• UniProt ID: P11277
• Cytogenetics: 14q23.3
• Protein Families: Druggable Genome
• MW: 267.6 kDa
• Gene Summary: This locus encodes a member of the spectrin gene family. Spectrin proteins, along with ankyrin, play a role in cell membrane organization and stability. The protein encoded by this locus functions in stability of erythrocyte membranes, and mutations in this gene have been associated with spherocytosis type 2, hereditary elliptocytosis, and neonatal hemolytic anemia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]