KRTAP13-1 (NM_181599) Human Tagged ORF Clone

CAT#: RC216152

KRTAP13 (Myc-DDK-tagged)-Human keratin associated protein 13-1 (KRTAP13-1)

Product Images

Circular map for RC216152

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KRTAP13-1
• Synonyms: KAP13.1; KRTAP13.1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216152 representing NM_181599
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGCATGCAAACTCAGAATCTTCTCAGTGTAACTCAGCTGAACTCACATCTCCCATCAACATGTCCT
  ACAACTGCTGCTCTGGAAACTTCTCCTCCCGCTCCTGTGGTGGCTACCTGCACTACCCAGCCTCCTCCTG
  TGGCTTTTCCTACCCCAGCAACCAGGTCTACAGCACTGACCTCTGCTCTCCCAGCACGTGCCAGCTGGGT
  TCCTCTCTCTATAGGGGCTGTCAGCAGACCTGCTGGGAGCCCACCAGCTGCCAGACATCCTATGTGGAGT
  CCAGCCCCTGCCAGACCTCCTGCTACCGTCCCAGAACCTCCTTGCTCTGCAGTCCCTGCCAGACAACTTA
  CTCTGGGTCTCTAGGCTTTGGATCCAGCAGCTGCCGCTCCCTGGGCTATGGATCGAGGAGCTGCTACTCA
  GTGGGCTGTGGGTCCAGTGGCTTCAGATCCCTGGGTTATGGAGGCTGTGGCTTCCCTTCCCTGGGCTATG
  GCGTTGGATTCTGCCGCCCAACCTACTTGGCTTCTAGGAGCTGCCAGTCTTCTTGCTACAGACCAACTTG
  TGGATCAGGCTTCTACTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216152 representing NM_181599
  Red=Cloning site Green=Tags(s)
  
  MWHANSESSQCNSAELTSPINMSYNCCSGNFSSRSCGGYLHYPASSCGFSYPSNQVYSTDLCSPSTCQLG
  SSLYRGCQQTCWEPTSCQTSYVESSPCQTSCYRPRTSLLCSPCQTTYSGSLGFGSSSCRSLGYGSRSCYS
  VGCGSSGFRSLGYGGCGFPSLGYGVGFCRPTYLASRSCQSSCYRPTCGSGFYY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181599
• ORF Size: 579 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181599.1, NP_853630.1
• RefSeq Size: 763 bp
• RefSeq ORF: 519 bp
• Locus ID: 140258
• UniProt ID: Q8IUC0
• Cytogenetics: 21q22.11
• MW: 21.1 kDa
• Gene Summary: Hair keratins and hair keratin-associated proteins (KAPs), such as KRTAP13-1, are the main structural proteins of hair fibers (Rogers et al., 2002 [PubMed 12359730]).[supplied by OMIM, Mar 2008]