KIAA1543 (CAMSAP3) (NM_001080429) Human Tagged ORF Clone

CAT#: RC216127

CAMSAP3 (Myc-DDK-tagged)-Human KIAA1543 (KIAA1543), transcript variant 1

Product Images

Circular map for RC216127

• 

  Western blot validation of overexpression lysate (Cat# LY421646) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216127 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIAA1543
• Synonyms: KIAA1543; NEZHA; PPP1R80
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216127 representing NM_001080429
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGAGGCGGCGCCCCCCGGGCCCGGGCCGCTGCGGAGGACCTTTCTAGTGCCCGAGATCAAGTCGC
  TGGACCAGTACGATTTCTCGCGGGCCAAGGCGGCGGCCAGCCTGGCGTGGGTGCTGCGGGCCGCGTTCGG
  GGGCGCAGAGCACGTGCCCCCGGAGCTGTGGGAGCCCTTCTATACCGACCAGTACGCGCAGGAGCATGTG
  AAGCCCCCGGTGACACGGCTGCTGCTCTCAGCCGAGCTCTACTGCAGAGCCTGGCGCCAGGCACTGCCAC
  AGCTTGAAACACCCCCCAACCCCTCTGCACTGCTGGCCCTGCTGGCGCGGAGGGGCACAGTGCCTGCTTT
  GCCCGAGCGCCCGGTGCGCGAGGCCGACCTGAGGCACCAGCCCATTCTCATGGGAGCCCACCTAGCTGTC
  ATTGATGCCCTCATGGCTGCCTTTGCCTTCGAGTGGACAAAGACCCTGCCAGGTCCCTTGGCCCTGACCA
  GCTTGGAGCACAAGCTGCTTTTCTGGGTGGACACGACCGTCCGGCGGCTGCAGGAGAAGACCGAGCAGGA
  AGCGGCCCAGCGAGCCTCTCCAGCAGCCCCTGCAGACGGGGCGGCCCCGGCGCAGCCCTCGTGCCCTACG
  CGCTGGTACTGGAAGCTGGTTCCTCACGCAATTGCCTTCTGTTTGAAGGAGTCGGGGAGCAAACCCCCCA
  TGATCCGATACCGCAAGGACCGTGTGGTGGCGCGACGTGCCCCCTGCTTCCCGACGGTGACCAGCCTCCA
  GGACCTGGCCAGTGGGGCCGCGCTGGCCGCCACCATCCACTGCTATTGTCCCCAGCTGCTTCGACTTGAG
  GAGGTGTGCTTGAAGGACCCCATGTCTGTGGCGGACAGCCTGTACAACCTCCAGCTCGTGCAGGATTTCT
  GTGCCTCTCGCCTTCCTCGTGGCTGCCCCCTGTCCCTTGAGGACTTGCTGTACGTCCCACCGCCACTCAA
  GGTCAACTTGGTGGTGATGCTGGCCGAGTTGTTCATGTGTTTTGAGGTGCTCAAGCCCGACTTTGTGCAA
  GTGAAGGACTTGCCCGATGGTCACGCTGCCTCCCCCCGGGGCACTGAGGCCTCCCCACCTCAGAACAACA
  GCGGCAGTAGTTCTCCTGTCTTCACCTTCCGCCACCCGCTTCTGTCATCTGGTGGCCCCCAGTCCCCACT
  CCGAGGATCCACAGGCTCCCTGAAGTCTTCCCCGTCCATGTCCCATATGGAGGCCCTGGGCAAGGCCTGG
  AACCGGCAGCTCAGCCGTCCCCTCTCCCAGGCTGTGTCATTCAGCACCCCCTTTGGCCTGGACAGCGACG
  TGGATGTCGTCATGGGAGACCCTGTGCTCCTCCGCTCTGTGAGCTCGGACAGCCTGGGCCCCCCGCGTCC
  CGCGCCGGCCAGGACCCCCACCCAGCCACCCCCGGAGCCTGGTGACCTGCCCACCATCGAGGAAGCTCTG
  CAGATCATCCACAGTGCCGAGCCCCGGCTCCTCCCAGATGGGGCGGCCGACGGCAGCTTCTACCTCCACT
  CCCCTGAGGGGCCCTCCAAGCCATCCCTGGCCTCCCCCTACCTGCCCGAGGGGACCTCCAAACCACTGTC
  CGACAGGCCCACCAAAGCACCAGTGTACATGCCACACCCCGAGACCCCCTCGAAACCATCTCCCTGTCTG
  GTGGGGGAGGCATCGAAACCGCCAGCCCCATCCGAGGGGTCCCCGAAGGCGGTGGCTTCGTCCCCAGCAG
  CCACCAACTCCGAGGTGAAAATGACCAGCTTTGCAGAACGCAAGAAACAGCTGGTGAAGGCAGAGGCTGA
  GGCCGGAGCGGGGTCCCCCACGTCCACTCCGGCCCCGCCGGAGGCCCTGAGCTCGGAGATGAGTGAGCTC
  AGCGCCCGGCTGGAGGAGAAACGCAGAGCCATCGAGGCTCAGAAGCGACGGATTGAGGCCATATTCGCCA
  AGCACCGCCAGCGGCTGGGCAAAAGCGCCTTCCTGCAGGTGCAGCCGCGGGAAGCCTCTGGGGAGGCGGA
  AGCAGAGGCGGAGGAGGCCGATTCCGGTCCAGTCCCTGGTGGGGAGCGGCCCGCAGGCGAGGGCCAGGGT
  GAGCCAACCTCACGGCCCAAGGCAGTGACCTTCTCGCCAGACCTGGGCCCGGTGCCCCACGAGGGGCTGG
  GGGAATACAATCGAGCGGTCAGCAAGCTGAGTGCCGCCTTGAGCTCGCTGCAGCGGGACATGCAGAGGCT
  CACGGACCAGCAGCAGCGGCTCCTGGCCCCGCCCGAGGCCCCCGGATCCGCCCCACCACCTGCTGCGTGG
  GTCATCCCTGGCCCCACGACGGGGCCCAAAGCTGCATCCCCCAGCCCCGCCCGGCGAGTCCCGGCCACCC
  GGCGCAGCCCTGGGCCCGGGCCCAGCCAGTCACCCCGCAGCCCGAAACACACGCGGCCAGCGGAGCTGCG
  GCTGGCACCCTTGACCAGGGTGCTTACGCCACCCCACGACGTAGACAGCCTCCCCCACCTGCGCAAGTTC
  TCGCCGAGCCAGGTGCCCGTGCAGACGCGCTCTTCCATCCTCCTGGCGGAGGAGACGCCCCCCGAGGAGC
  CAGCCGCCCGGCCGGGCCTCATCGAGATCCCGCTGGGCAGCCTGGCAGATCCCGCCGCCGAGGACGAGGG
  AGACGGGAGCCCCGCTGGTGCTGAGGATTCCTTGGAGGAGGAGGCGTCTTCGGAGGGGGAGCCCCGGGTG
  GGGCTGGGGTTCTTCTACAAGGATGAAGACAAGCCTGAGGACGAGATGGCCCAAAAGCGGGCCAGCCTGC
  TGGAGCGGCAGCAGCGGCGAGCAGAGGAGGCGCGGCGGCGCAAGCAGTGGCAGGAGGTGGAGAAGGAACA
  GCGGAGGGAGGAGGCCGCGAGGCTGGCCCAAGAGGAGGCCCCGGGCCCAGCCCCGCTTGTGTCCGCAGTC
  CCGATGGCGACTCCAGCCCCTGCTGCCCGGGCTCCAGCCGAGGAGGAGGTGGGCCCCCGGAAGGGGGACT
  TCACGCGGCAGGAGTACGAGCGCCGGGCCCAGCTGAAGCTGATGGACGACCTCGATAAGGTGCTGCGGCC
  CCGGGCTGCGGGGTCCGGGGGTCCAGGTCGGGGCGGGCGGAGGGCCACCCGGCCTCGCTCGGGTTGCTGT
  GACGACTCAGCCCTGGCACGAAGCCCAGCCCGCGGCCTGCTGGGCTCTCGGCTGAGCAAAATCTATTCCC
  AGTCCACCCTGTCACTGTCCACTGTGGCCAACGAGGCCCACAATAACCTCGGGGTGAAGAGGCCCACGTC
  TCGGGCTCCCTCCCCGTCAGGTCTCATGTCCCCAAGCCGCCTGCCTGGAAGCCGCGAACGGGACTGGGAA
  AATGGCAGCAATGCCTCCTCCCCAGCGTCAGTGCCCGAGTACACAGGTCCACGGCTGTACAAAGAACCCA
  GCGCCAAGTCCAACAAGTTCATCATCCACAATGCCCTATCACACTGCTGCCTGGCGGGCAAGGTGAACGA
  ACCGCAGAAGAATCGCATTCTGGAGGAAATTGAGAAAAGCAAGGCCAACCACTTCCTGATCCTCTTTCGC
  GACTCGAGCTGCCAGTTCCGGGCGCTCTACACGCTGTCGGGGGAGACAGAGGAGCTGTCGCGGCTGGCAG
  GGTATGGGCCCCGGACCGTCACGCCCGCCATGGTGGAAGGCATCTACAAGTACAACTCGGACCGCAAGCG
  CTTCACCCAGATCCCCGCCAAGACCATGTCCATGAGCGTCGATGCCTTCACCATCCAGGGACACCTCTGG
  CAGGGCAAGAAACCCACCACTCCCAAGAAGGGCGGCGGCACCCCCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216127 representing NM_001080429
  Red=Cloning site Green=Tags(s)
  
  MVEAAPPGPGPLRRTFLVPEIKSLDQYDFSRAKAAASLAWVLRAAFGGAEHVPPELWEPFYTDQYAQEHV
  KPPVTRLLLSAELYCRAWRQALPQLETPPNPSALLALLARRGTVPALPERPVREADLRHQPILMGAHLAV
  IDALMAAFAFEWTKTLPGPLALTSLEHKLLFWVDTTVRRLQEKTEQEAAQRASPAAPADGAAPAQPSCPT
  RWYWKLVPHAIAFCLKESGSKPPMIRYRKDRVVARRAPCFPTVTSLQDLASGAALAATIHCYCPQLLRLE
  EVCLKDPMSVADSLYNLQLVQDFCASRLPRGCPLSLEDLLYVPPPLKVNLVVMLAELFMCFEVLKPDFVQ
  VKDLPDGHAASPRGTEASPPQNNSGSSSPVFTFRHPLLSSGGPQSPLRGSTGSLKSSPSMSHMEALGKAW
  NRQLSRPLSQAVSFSTPFGLDSDVDVVMGDPVLLRSVSSDSLGPPRPAPARTPTQPPPEPGDLPTIEEAL
  QIIHSAEPRLLPDGAADGSFYLHSPEGPSKPSLASPYLPEGTSKPLSDRPTKAPVYMPHPETPSKPSPCL
  VGEASKPPAPSEGSPKAVASSPAATNSEVKMTSFAERKKQLVKAEAEAGAGSPTSTPAPPEALSSEMSEL
  SARLEEKRRAIEAQKRRIEAIFAKHRQRLGKSAFLQVQPREASGEAEAEAEEADSGPVPGGERPAGEGQG
  EPTSRPKAVTFSPDLGPVPHEGLGEYNRAVSKLSAALSSLQRDMQRLTDQQQRLLAPPEAPGSAPPPAAW
  VIPGPTTGPKAASPSPARRVPATRRSPGPGPSQSPRSPKHTRPAELRLAPLTRVLTPPHDVDSLPHLRKF
  SPSQVPVQTRSSILLAEETPPEEPAARPGLIEIPLGSLADPAAEDEGDGSPAGAEDSLEEEASSEGEPRV
  GLGFFYKDEDKPEDEMAQKRASLLERQQRRAEEARRRKQWQEVEKEQRREEAARLAQEEAPGPAPLVSAV
  PMATPAPAARAPAEEEVGPRKGDFTRQEYERRAQLKLMDDLDKVLRPRAAGSGGPGRGGRRATRPRSGCC
  DDSALARSPARGLLGSRLSKIYSQSTLSLSTVANEAHNNLGVKRPTSRAPSPSGLMSPSRLPGSRERDWE
  NGSNASSPASVPEYTGPRLYKEPSAKSNKFIIHNALSHCCLAGKVNEPQKNRILEEIEKSKANHFLILFR
  DSSCQFRALYTLSGETEELSRLAGYGPRTVTPAMVEGIYKYNSDRKRFTQIPAKTMSMSVDAFTIQGHLW
  QGKKPTTPKKGGGTPK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001080429
• ORF Size: 3828 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080429.3
• RefSeq Size: 4084 bp
• RefSeq ORF: 3831 bp
• Locus ID: 57662
• UniProt ID: Q9P1Y5
• Cytogenetics: 19p13.2
• MW: 137.7 kDa
• Gene Summary: Key microtubule-organizing protein that specifically binds the minus-end of non-centrosomal microtubules and regulates their dynamics and organization (PubMed:19041755, PubMed:23169647). Specifically recognizes growing microtubule minus-ends and autonomously decorates and stabilizes microtubule lattice formed by microtubule minus-end polymerization (PubMed:24486153). Acts on free microtubule minus-ends that are not capped by microtubule-nucleating proteins or other factors and protects microtubule minus-ends from depolymerization (PubMed:24486153). In addition, it also reduces the velocity of microtubule polymerization (PubMed:24486153). Required for the biogenesis and the maintenance of zonula adherens by anchoring the minus-end of microtubules to zonula adherens and by recruiting the kinesin KIFC3 to those junctional sites (PubMed:19041755). Required for orienting the apical-to-basal polarity of microtubules in epithelial cells: acts by tethering non-centrosomal microtubules to the apical cortex, leading to their longitudinal orientation (PubMed:27802168, PubMed:26715742). Plays a key role in early embryos, which lack centrosomes: accumulates at the microtubule bridges that connect pairs of cells and enables the formation of a non-centrosomal microtubule-organizing center that directs intracellular transport in the early embryo (By similarity). Couples non-centrosomal microtubules with actin: interaction with MACF1 at the minus ends of non-centrosomal microtubules, tethers the microtubules to actin filaments, regulating focal adhesion size and cell migration (PubMed:27693509). Plays a key role in the generation of non-centrosomal microtubules by accumulating in the pericentrosomal region and cooperating with KATNA1 to release non-centrosomal microtubules from the centrosome (PubMed:28386021). Through the microtubule cytoskeleton, also regulates the organization of cellular organelles including the Golgi and the early endosomes (PubMed:28089391). Through interaction with AKAP9, involved in translocation of Golgi vesicles in epithelial cells, where microtubules are mainly non-centrosomal (PubMed:28089391).[UniProtKB/Swiss-Prot Function]