Vasopressin V1b receptor (AVPR1B) (NM_000707) Human Tagged ORF Clone

CAT#: RC215919

AVPR1B (Myc-DDK-tagged)-Human arginine vasopressin receptor 1B (AVPR1B)

Product Images

Circular map for RC215919

• 

  Western blot validation of overexpression lysate (Cat# LY400237) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215919 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Vasopressin V1b receptor
• Synonyms: AVPR3; V1bR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215919 representing NM_000707
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTCTGGGCCTCTGTGGGATGCCAACCCCACCCCTCGGGGCACCCTCTCTGCCCCCAATGCCACAA
  CACCCTGGCTGGGCCGGGATGAGGAGCTGGCCAAGGTGGAGATCGGAGTCCTGGCCACTGTCCTGGTGCT
  GGCGACCGGGGGCAACCTGGCTGTGCTGCTGACCCTGGGCCAGCTGGGCCGCAAGCGCTCCCGCATGCAC
  CTGTTCGTGCTGCACTTAGCCCTGACAGACCTGGCCGTGGCGCTCTTCCAGGTGCTGCCACAGCTGCTGT
  GGGACATCACCTACCGCTTCCAGGGCCCCGACCTCCTGTGCAGGGCCGTCAAGTACCTGCAGGTGCTCAG
  CATGTTTGCCTCCACCTACATGCTGCTGGCCATGACGCTGGACCGCTACCTGGCTGTCTGTCACCCCCTG
  CGCAGCCTCCAGCAGCCAGGCCAGTCCACCTACCTGCTCATCGCTGCTCCCTGGCTGCTGGCCGCCATCT
  TCAGCCTCCCTCAAGTCTTCATTTTTTCCCTGCGGGAGGTGATCCAGGGCTCAGGGGTGCTGGACTGCTG
  GGCAGACTTCGGCTTCCCTTGGGGGCCACGGGCCTACCTCACCTGGACCACCCTGGCTATCTTCGTTCCG
  CCGGTGACCATGCTCACGGCCTGCTACAGCCTCATCTGCCATGAGATCTGTAAAAACCTAAAAGTCAAGA
  CACAGGCCTGGCGGGTGGGAGGAGGGGGCTGGAGGACTTGGGACAGGCCCTCACCTTCCACCTTAGCTGC
  CACCACTCGGGGGCTGCCATCTCGGGTCAGCAGCATCAACACCATCTCACGGGCCAAGATCCGAACAGTG
  AAGATGACCTTTGTCATCGTGCTGGCCTACATCGCTTGCTGGGCTCCCTTCTTCAGTGTCCAGATGTGGT
  CCGTGTGGGACAAGAATGTCCCTGATGAAGATTCCACCAATGTGGCTTTCACCATCTCTATGCTTTTGGG
  CAACCTCAACAGCTGCTGCAACCCCTGGATCTACATGGGCTTCAACAGCCACCTGTTACCGCGGCCCCTG
  CGTCACCTTGCCTGCTGTGGGGGTCCCCAGCCCAGGATGCGCCGGCGGCTCTCCGACGGCAGCCTCTCGA
  GCCGCCACACCACGCTGCTGACCCGCTCCAGCTGCCCGGCCACCCTCAGCCTCAGCCTCAGCCTAACCCT
  CAGTGGGAGGCCCAGGCCTGAAGAGTCACCAAGGGACTTGGAGCTGGCAGATGGGGAAGGCACCGCTGAG
  ACCATCATCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215919 representing NM_000707
  Red=Cloning site Green=Tags(s)
  
  MDSGPLWDANPTPRGTLSAPNATTPWLGRDEELAKVEIGVLATVLVLATGGNLAVLLTLGQLGRKRSRMH
  LFVLHLALTDLAVALFQVLPQLLWDITYRFQGPDLLCRAVKYLQVLSMFASTYMLLAMTLDRYLAVCHPL
  RSLQQPGQSTYLLIAAPWLLAAIFSLPQVFIFSLREVIQGSGVLDCWADFGFPWGPRAYLTWTTLAIFVP
  PVTMLTACYSLICHEICKNLKVKTQAWRVGGGGWRTWDRPSPSTLAATTRGLPSRVSSINTISRAKIRTV
  KMTFVIVLAYIACWAPFFSVQMWSVWDKNVPDEDSTNVAFTISMLLGNLNSCCNPWIYMGFNSHLLPRPL
  RHLACCGGPQPRMRRRLSDGSLSSRHTTLLTRSSCPATLSLSLSLTLSGRPRPEESPRDLELADGEGTAE
  TIIF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000707
• ORF Size: 1272 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_000707.5
• RefSeq Size: 2273 bp
• RefSeq ORF: 1275 bp
• Locus ID: 553
• UniProt ID: P47901
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Vascular smooth muscle contraction
• MW: 46.8 kDa
• Gene Summary: The protein encoded by this gene acts as receptor for arginine vasopressin. This receptor belongs to the subfamily of G-protein coupled receptors which includes AVPR1A, V2R and OXT receptors. Its activity is mediated by G proteins which stimulate a phosphatidylinositol-calcium second messenger system. The receptor is primarily located in the anterior pituitary, where it stimulates ACTH release. It is expressed at high levels in ACTH-secreting pituitary adenomas as well as in bronchial carcinoids responsible for the ectopic ACTH syndrome. A spliced antisense transcript of this gene has been reported but its function is not known. [provided by RefSeq, Jul 2008]