FOXK1 (NM_001037165) Human Tagged ORF Clone

CAT#: RC215896

FOXK1 (Myc-DDK-tagged)-Human forkhead box K1 (FOXK1)

Product Images

Circular map for RC215896

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FOXK1
• Synonyms: FOXK1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215896 representing NM_001037165
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCAGATCTCAAGCTTAACTAGCTAGCGGACCGAC
  
  ATGGCCGAAGTCGGCGAGGACAGCGGCGCCCGCGCCCTGCTCGCGCTGCGCTCGGCGCCCTGCAGCCCAG
  TGCTGTGCGCCGCAGCCGCCGCCGCCGCCTTCCCCGCGGCCGCACCCCCGCCGGCCCCCGCGCAGCCCCA
  GCCTCCGCCCGGGCCGCCGCCGCCGCCGCCACCGCCGCTGCCTCCGGGCGCGATCGCGGGCGCGGGCTCC
  TCCGGGGGCTCCTCCGGGGTATCCGGGGACTCCGCGGTCGCGGGCGCGGCGCCGGCCCTGGTGGCCGCGG
  CGGCCGCCTCGGTACGGCAGAGCCCGGGGCCGGCGCTGGCGCGGCTGGAGGGCCGCGAGTTCGAGTTCCT
  CATGCGCCAGCCCAGCGTCACCATCGGCCGCAACTCGTCGCAGGGCTCGGTGGACTTGAGCATGGGCCTG
  TCCAGCTTCATCTCGCGGCGCCACCTGCAGCTCAGCTTCCAGGAGCCGCACTTCTACCTGCGCTGCCTCG
  GCAAGAACGGCGTCTTCGTGGACGGGGCCTTCCAGAGACGCGGCGCGCCCGCCCTGCAGCTGCCCAAGCA
  GTGTACCTTCCGGTTTCCCAGCACGGCCATCAAGATCCAGTTCACGTCGCTCTATCACAAAGAAGAGGCC
  CCAGCCTCCCCGCTGCGGCCACTGTACCCCCAGATCTCCCCTCTGAAGATCCACATCCCGGAGCCGGACC
  TCCGGAGCATGGTCAGCCCCGTCCCCTCCCCGACGGGCACCATCAGTGTCCCCAACTCCTGCCCAGCCAG
  TCCACGCGGTGCCGGCTCCTCCAGTTACCGCTTTGTGCAGAACGTGACCTCGGACCTGCAGCTGGCAGCA
  GAGTTTGCAGCAAAGGCCGCGTCGGAGCAGCAGGCAGACACGTCTGGAGGAGACAGCCCCAAGGATGAGT
  CAAAGCCGCCGTTCTCCTACGCGCAGCTGATCGTGCAGGCCATCTCCTCCGCCCAGGACCGGCAGCTGAC
  CCTGAGCGGGATCTACGCCCACATCACCAAGCATTACCCCTACTACCGGACGGCCGACAAAGGCTGGCAG
  AATTCTATCCGGCACAACCTCTCTTTGAACCGTTACTTTATCAAAGTCCCACGTTCCCAGGAGGAGCCTG
  GGAAGGGGTCCTTTTGGCGAATAGACCCTGCCTCTGAAGCCAAGCTCGTGGAACAGGCATTCCGGAAACG
  GAGGCAGAGGGGTGTCTCCTGCTTCCGCACCCCCTTCGGGCCTCTGTCCTCAAGGAGCGCTCCAGCTTCG
  CCCACACACCCCGGGCTGATGTCCCCTCGCTCCGGCGGCCTGCAGACCCCAGAGTGCCTGTCTCGGGAGG
  GCTCCCCCATTCCACACGACCCTGAGTTTGGGTCCAAGTTAGCTTCTGTCCCAGAGTACCGGTATTCCCA
  AAGCGCACCCGGCTCCCCCGTCAGCGCCCAGCCAGTGATCATGGCCGTGCCTCCCCGACCGTCCAGCCTC
  GTGGCCAAGCCCGTGGCCTACATGCCCGCCTCCATCGTAACCTCACAGCAGCCCGCGGGCCACGCCATCC
  ACGTCGTGCAGCAGGCCCCCACCGTCACCATGGTCAGGGTGGTCACCACATCTGCCAACTCGGCCAACGG
  ATACATCCTCACCAGCCAGGGCGCGGCGGGGGGCTCCCATGATGCGGCGGGCGCAGCCGTGCTGGACCTG
  GGCAGCGAGGCCAGAGGCCTGGAGGAGAAACCCACCATTGCGTTTGCCACAATCCCCGCGGCTGGTGGAG
  TCATCCAGACGGTGGCCAGCCAGATGGCCCCCGGGGTCCCCGGACACACGGTCACCATCCTGCAGCCCGC
  CACACCCGTGACCCTCGGGCAGCACCACCTTCCAGTCCGGGCCGTGACCCAGAACGGAAAGCATGCGGTT
  CCCACGAACAGTTTAGCCGGCAACGCTTACGCCCTCACCAGCCCTTTGCAGCTCCTTGCGACCCAAGCGA
  GTTCATCCGCGCCGGTGGTGGTCACCCGGGTGTGCGAGGTGGGGCCCAAGGAGCCAGCAGCAGCCGTCGC
  GGCCACGGCCACCACCACCCCAGCCACTGCCACCACCGCCTCTGCCTCCGCCTCTTCCACTGGAGAGCCC
  GAGGTCAAAAGGTCCCGGGTGGAGGAGCCCAGTGGGGCTGTAACCACACCGGCTGGAGTGATCGCAGCTG
  CCGGCCCCCAGGGGCCAGGCACCGGGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215896 representing NM_001037165
  Red=Cloning site Green=Tags(s)
  
  MAEVGEDSGARALLALRSAPCSPVLCAAAAAAAFPAAAPPPAPAQPQPPPGPPPPPPPPLPPGAIAGAGS
  SGGSSGVSGDSAVAGAAPALVAAAAASVRQSPGPALARLEGREFEFLMRQPSVTIGRNSSQGSVDLSMGL
  SSFISRRHLQLSFQEPHFYLRCLGKNGVFVDGAFQRRGAPALQLPKQCTFRFPSTAIKIQFTSLYHKEEA
  PASPLRPLYPQISPLKIHIPEPDLRSMVSPVPSPTGTISVPNSCPASPRGAGSSSYRFVQNVTSDLQLAA
  EFAAKAASEQQADTSGGDSPKDESKPPFSYAQLIVQAISSAQDRQLTLSGIYAHITKHYPYYRTADKGWQ
  NSIRHNLSLNRYFIKVPRSQEEPGKGSFWRIDPASEAKLVEQAFRKRRQRGVSCFRTPFGPLSSRSAPAS
  PTHPGLMSPRSGGLQTPECLSREGSPIPHDPEFGSKLASVPEYRYSQSAPGSPVSAQPVIMAVPPRPSSL
  VAKPVAYMPASIVTSQQPAGHAIHVVQQAPTVTMVRVVTTSANSANGYILTSQGAAGGSHDAAGAAVLDL
  GSEARGLEEKPTIAFATIPAAGGVIQTVASQMAPGVPGHTVTILQPATPVTLGQHHLPVRAVTQNGKHAV
  PTNSLAGNAYALTSPLQLLATQASSSAPVVVTRVCEVGPKEPAAAVAATATTTPATATTASASASSTGEP
  EVKRSRVEEPSGAVTTPAGVIAAAGPQGPGTGE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: RsrII-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001037165
• ORF Size: 2199 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001037165.1, NP_001032242.1
• RefSeq Size: 11191 bp
• RefSeq ORF: 2202 bp
• Locus ID: 221937
• UniProt ID: P85037
• Cytogenetics: 7p22.1
• MW: 75.3 kDa
• Gene Summary: Transcriptional regulator involved in different processes such as glucose metabolism, aerobic glycolysis, muscle cell differentiation and autophagy (By similarity). Recognizes and binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can both act as a transcription activator or repressor, depending on the context (PubMed:17670796). Together with FOXK2, acts as a key regulator of metabolic reprogramming towards aerobic glycolysis, a process in which glucose is converted to lactate in the presence of oxygen (By similarity). Acts by promoting expression of enzymes for glycolysis (such as hexokinase-2 (HK2), phosphofructokinase, pyruvate kinase (PKLR) and lactate dehydrogenase), while suppressing further oxidation of pyruvate in the mitochondria by up-regulating pyruvate dehydrogenase kinases PDK1 and PDK4 (By similarity). Probably plays a role in gluconeogenesis during overnight fasting, when lactate from white adipose tissue and muscle is the main substrate (By similarity). Involved in mTORC1-mediated metabolic reprogramming: in response to mTORC1 signaling, translocates into the nucleus and regulates the expression of genes associated with glycolysis and downstream anabolic pathways, such as HIF1A, thereby regulating glucose metabolism (By similarity). Together with FOXK2, acts as a negative regulator of autophagy in skeletal muscle: in response to starvation, enters the nucleus, binds the promoters of autophagy genes and represses their expression, preventing proteolysis of skeletal muscle proteins (By similarity). Acts as a transcriptional regulator of the myogenic progenitor cell population in skeletal muscle (By similarity). Binds to the upstream enhancer region (CCAC box) of myoglobin (MB) gene, regulating the myogenic progenitor cell population (By similarity). Promotes muscle progenitor cell proliferation by repressing the transcriptional activity of FOXO4, thereby inhibiting myogenic differentiation (By similarity). Involved in remodeling processes of adult muscles that occur in response to physiological stimuli (By similarity). Required to correct temporal orchestration of molecular and cellular events necessary for muscle repair (By similarity). Represses myogenic differentiation by inhibiting MEFC activity (By similarity). Positively regulates Wnt/beta-catenin signaling by translocating DVL into the nucleus (PubMed:25805136). Reduces virus replication, probably by binding the interferon stimulated response element (ISRE) to promote antiviral gene expression (PubMed:25852164).[UniProtKB/Swiss-Prot Function]