FUT2 (NM_001097638) Human Tagged ORF Clone

CAT#: RC215830

FUT2 (Myc-DDK-tagged)-Human fucosyltransferase 2 (secretor status included) (FUT2), transcript variant 2

Product Images

Circular map for RC215830

• 

  Western blot validation of overexpression lysate (Cat# LY424669) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219898 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FUT2
• Synonyms: B12QTL1; SE; Se2; SEC2; sej
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215830 representing NM_001097638
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGTCGTTCAGATGCCTTTCTCCTTTCCCATGGCCCACTTCATCCTCTTTGTCTTTACGGTTTCCA
  CTATATTTCACGTTCAGCAGCGGCTAGCGAAGATTCAAGCCATGTGGGAGTTACCGGTGCAGATACCAGT
  GCTAGCCTCAACATCAAAGGCACTGGGACCCAGCCAGCTCAGGGGGATGTGGACGATCAATGCAATAGGC
  CGCCTGGGGAACCAGATGGGCGAGTACGCCACACTGTACGCCCTGGCCAAGATGAACGGGCGGCCCGCCT
  TCATCCCGGCCCAGATGCACAGCACCCTGGCCCCCATCTTCAGAATCACCCTGCCGGTGCTGCACAGCGC
  CACGGCCAGCAGGATCCCCTGGCAGAACTACCACCTGAACGACTGGATGGAGGAGGAATACCGCCACATC
  CCGGGGGAGTACGTCCGCTTCACCGGCTACCCCTGCTCCTGGACCTTCTACCACCACCTCCGCCAGGAGA
  TCCTCCAGGAGTTCACCCTGCACGACCACGTGCGGGAGGAGGCCCAGAAGTTCCTGCGGGGCCTGCAGGT
  GAACGGGAGCCGGCCGGGCACCTTTGTAGGGGTCCATGTTCGCCGAGGGGACTATGTCCATGTCATGCCA
  AAAGTGTGGAAGGGGGTGGTGGCCGACCGGCGATACCTACAGCAGGCCCTGGACTGGTTCCGAGCTCGCT
  ACAGCTCCCTCATCTTCGTGGTCACCAGTAATGGCATGGCCTGGTGTCGGGAGAACATTGACACCTCCCA
  CGGTGATGTGGTGTTTGCTGGCGATGGCATTGAGGGCTCACCTGCCAAAGATTTTGCTCTACTCACACAG
  TGTAACCACACCATCATGACCATTGGGACGTTCGGGATCTGGGCCGCATACCTCACGGGCGGAGACACCA
  TCTACCTGGCCAATTACACCCTCCCCGACTCCCCTTTCCTCAAAATCTTTAAGCCAGAGGCAGCCTTCCT
  GCCGGAGTGGACAGGGATTGCCGCAGACCTGTCCCCCTTACTCAAGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215830 representing NM_001097638
  Red=Cloning site Green=Tags(s)
  
  MLVVQMPFSFPMAHFILFVFTVSTIFHVQQRLAKIQAMWELPVQIPVLASTSKALGPSQLRGMWTINAIG
  RLGNQMGEYATLYALAKMNGRPAFIPAQMHSTLAPIFRITLPVLHSATASRIPWQNYHLNDWMEEEYRHI
  PGEYVRFTGYPCSWTFYHHLRQEILQEFTLHDHVREEAQKFLRGLQVNGSRPGTFVGVHVRRGDYVHVMP
  KVWKGVVADRRYLQQALDWFRARYSSLIFVVTSNGMAWCRENIDTSHGDVVFAGDGIEGSPAKDFALLTQ
  CNHTIMTIGTFGIWAAYLTGGDTIYLANYTLPDSPFLKIFKPEAAFLPEWTGIAADLSPLLKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001097638
• ORF Size: 1029 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001097638.3
• RefSeq Size: 3105 bp
• RefSeq ORF: 1032 bp
• Locus ID: 2524
• UniProt ID: Q10981
• Cytogenetics: 19q13.33
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - globo series, Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways
• MW: 38.8 kDa
• Gene Summary: The protein encoded by this gene is a Golgi stack membrane protein that is involved in the creation of a precursor of the H antigen, which is required for the final step in the soluble A and B antigen synthesis pathway. This gene is one of two encoding the galactoside 2-L-fucosyltransferase enzyme. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]