USP31 (NM_020718) Human Tagged ORF Clone

CAT#: RC215820

USP31 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 31 (USP31)

Product Images

Circular map for RC215820

• 

  Western blot validation of overexpression lysate (Cat# LY412348) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215820 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: USP31
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215820 representing NM_020718
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCAAGGTAACGGCGCCTGGGTCCGGGCCGCCGGCGGCGGCGAGCGGGAAGGAGAAGCGCTCCTTCA
  GCAAGCGGCTGTTTCGGAGCGGCCGCGCTGGCGGCGGCGGCGCGGGGGGCCCCGGGGCGTCCGGGCCGGC
  CGCGCCTTCCTCGCCCTCCTCGCCCTCCTCTGCACGCTCGGTGGGCAGCTTCATGAGCCGCGTTCTCAAG
  ACGCTGTCCACGCTCTCGCACCTTAGCTCTGAGGGCGCCGCCCCAGACCGCGGCGGCCTCCGCAGCTGCT
  TCCCGCCCGGGCCGGCCGCCGCGCCCACGCCGCCGCCGTGCCCGCCGCCGCCCGCCTCTCCCGCGCCGCC
  CGCTTGCGCCGCCGAGCCGGTGCCCGGCGTGGCGGGGCTCCGCAACCACGGCAACACGTGCTTCATGAAC
  GCCACGCTGCAGTGCCTCAGCAACACCGAGCTCTTCGCCGAGTACCTGGCGCTGGGCCAGTACCGGGCGG
  GGCGGCCCGAGCCCTCGCCTGACCCGGAGCAGCCTGCGGGCCGCGGCGCGCAGGGCCAGGGCGAGGTCAC
  TGAGCAGCTGGCGCACCTGGTGCGGGCCCTCTGGACCCTGGAGTACACCCCGCAGCACAGCCGCGACTTC
  AAGACTATTGTGTCAAAGAATGCACTGCAGTACCGGGGAAATTCCCAACATGATGCCCAGGAGTTTCTGC
  TGTGGCTTTTGGACCGAGTTCATGAAGACCTCAACCATTCAGTGAAGCAGAGTGGCCAGCCTCCTCTGAA
  GCCACCATCAGAGACTGATATGATGCCTGAGGGACCATCTTTCCCTGTCTGTAGCACTTTTGTACAAGAA
  CTCTTTCAAGCGCAATACAGATCTTCTTTGACGTGTCCTCATTGTCAGAAACAGAGCAACACTTTTGATC
  CTTTCCTTTGCATTTCTTTGCCAATTCCTCTGCCCCACACAAGGCCTCTCTATGTCACTGTAGTGTATCA
  AGGCAAATGTTCTCACTGCATGAGGATTGGTGTGGCCGTACCTCTGTCTGGGACTGTCGCCAGACTTCGG
  GAAGCAGTGTCTATGGAAACAAAGATCCCCACTGATCAGATTGTGTTAACAGAAATGTACTATGATGGGT
  TCCATCGTTCCTTTTGTGATACAGACGACCTGGAAACAGTCCATGAAAGCGACTGCATTTTTGCCTTTGA
  GACTCCCGAAATATTTAGGCCTGAAGGAATTCTCAGTCAAAGAGGAATTCATTTAAACAACAACCTAAAC
  CACTTGAAATTTGGCTTGGATTATCATAGACTGTCTTCTCCTACACAAACAGCAGCAAAGCAGGGGAAAA
  TGGATTCTCCCACATCAAGAGCAGGCAGCGACAAGATTGTCCTGTTGGTGTGTAACCGAGCCTGCACTGG
  GCAACAAGGGAAAAGATTTGGACTGCCTTTTGTGCTGCACTTAGAGAAGACAATAGCTTGGGACCTTCTG
  CAGAAGGAAATCTTGGAGAAGATGAAGTATTTCTTGAGGCCCACGGTTTGCATTCAGGTGTGTCCATTCA
  GCTTGCGTGTGGTCAGTGTTGTTGGAATAACATATTTGCTGCCCCAGGAGGAGCAGCCCTTGTGCCACCC
  AATAGTAGAAAGGGCATTAAAATCTTGTGGACCAGGTGGCACTGCTCATGTGAAATTAGTAGTCGAGTGG
  GACAAGGAGACAAGAGATTTCTTATTTGTAAATACTGAGGATGAGTATATTCCTGATGCAGAAAGTGTTC
  GTCTGCAAAGGGAGCGTCATCATCAGCCTCAAACCTGCACTTTATCCCAGTGTTTCCAACTGTACACCAA
  AGAGGAGCGGCTTGCCCCCGATGATGCCTGGCGTTGCCCACACTGTAAGCAGCTGCAGCAGGGAAGCATT
  ACGTTAAGCCTCTGGACTCTGCCTGATGTGCTTATTATACATCTAAAGAGATTTCGGCAGGAAGGAGACA
  GGCGCATGAAACTTCAGAACATGGTCAAATTCCCCTTGACTGGCCTGGACATGACACCTCACGTGGTTAA
  GAGGAGCCAGAGCAGCTGGAGTTTGCCATCGCATTGGTCCCCGTGGAGACGGCCCTATGGACTCGGGAGG
  GACCCTGAGGACTACATCTATGACCTGTATGCTGTGTGCAATCACCATGGCACCATGCAAGGGGGGCACT
  ACACAGCGTACTGTAAGAACTCTGTGGACGGCCTCTGGTACTGCTTCGATGACAGCGATGTGCAGCAGCT
  GTCAGAAGATGAGGTCTGCACGCAGACAGCATACATCCTCTTCTACCAGAGGCGGACAGCCATCCCGTCA
  TGGTCAGCCAACAGCTCGGTGGCAGGCTCCACAAGTTCTTCCCTGTGTGAACACTGGGTGAGCCGGCTCC
  CGGGCAGCAAGCCAGCCAGCGTGACCTCTGCAGCTTCCTCCAGACGCACCTCCCTGGCGTCGCTCTCTGA
  GTCCGTGGAGATGACTGGAGAAAGGAGTGAAGATGATGGAGGCTTTTCAACTCGACCATTTGTGAGAAGT
  GTCCAGCGTCAGAGTTTGTCATCCAGATCTTCTGTCACCAGCCCCTTGGCCGTCAATGAAAATTGCATGA
  GACCTTCATGGTCCCTGTCTGCTAAGCTGCAGATGCGCTCCAATTCTCCATCCCGATTTTCAGGGGATTC
  GCCAATTCACAGCTCTGCTTCCACCTTGGAGAAGATTGGGGAGGCAGCAGATGACAAGGTCTCCATCTCT
  TGCTTTGGTAGCTTGCGGAACCTTTCTAGCAGTTACCAGGAACCAAGCGACAGTCATAGTCGCCGTGAGC
  ACAAGGCTGTGGGCCGGGCCCCTCTGGCTGTCATGGAAGGCGTGTTCAAAGACGAATCGGACACCCGCAG
  ATTGAACTCCAGTGTCGTAGATACACAGAGCAAACATTCAGCACAAGGGGACCGCCTGCCCCCGCTCTCT
  GGTCCATTTGATAACAATAATCAGATCGCTTATGTGGATCAGAGCGACTCCGTAGACAGCTCTCCAGTCA
  AAGAGGTGAAAGCCCCCAGCCACCCAGGCTCACTCGCAAAGAAACCAGAGAGCACAACTAAGAGATCCCC
  CAGTTCCAAAGGCACTTCTGAGCCAGAGAAAAGCTTGCGGAAGGGGAGACCAGCCTTGGCAAGCCAGGAG
  TCATCCCTTTCAAGTACATCCCCTTCTTCTCCTCTTCCTGTAAAAGTCTCTCTAAAGCCCTCCCGCTCCC
  GCAGCAAAGCAGATTCTTCTTCCAGGGGCAGTGGACGGCATTCATCCCCTGCCCCTGCCCAACCCAAAAA
  GGAGTCATCCCCGAAATCTCAGGACTCCGTGTCATCTCCTTCGCCACAGAAGCAGAAGTCAGCCTCGGCC
  CTCACCTACACTGCTTCCTCCACATCTGCCAAAAAGGCCTCGGGCCCTGCCACAAGGAGCCCTTTCCCAC
  CTGGGAAGAGCAGGACTTCAGACCACAGCTTGAGTAGAGAGGGCTCCAGACAAAGCTTGGGTTCTGACAG
  AGCCAGCGCCACCTCCACCTCCAAACCCAATTCCCCTCGGGTGAGCCAGGCCCGAGCAGGGGAGGGCAGG
  GGGGCCGGGAAGCACGTGCGGAGCTCCTCCATGGCCAGCCTGCGCTCCCCCAGCACAAGCATCAAGTCTG
  GTTTGAAGAGGGACAGCAAGTCTGAGGACAAGGGGCTGTCCTTCTTCAAATCAGCCTTGAGACAGAAGGA
  AACCCGGCGCTCGACGGATCTTGGCAAGACAGCCTTGCTCTCTAAAAAGGCTGGTGGGAGCTCTGTTAAG
  TCTGTCTGTAAGAACACCGGGGACGACGAGGCAGAGAGAGGCCACCAGCCTCCAGCTTCCCAGCAGCCAA
  ATGCAAATACAACGGGAAAAGAGCAGCTTGTCACCAAGGACCCTGCTTCTGCCAAACATTCCCTGCTGTC
  CGCTCGCAAATCCAAGTCTTCCCAACTAGACTCTGGAGTTCCCTCGTCTCCGGGTGGCAGGCAGTCTGCA
  GAGAAATCCTCAAAAAAGTTATCTTCTAGCATGCAAACCTCTGCACGGCCTTCTCAAAAACCTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215820 representing NM_020718
  Red=Cloning site Green=Tags(s)
  
  MSKVTAPGSGPPAAASGKEKRSFSKRLFRSGRAGGGGAGGPGASGPAAPSSPSSPSSARSVGSFMSRVLK
  TLSTLSHLSSEGAAPDRGGLRSCFPPGPAAAPTPPPCPPPPASPAPPACAAEPVPGVAGLRNHGNTCFMN
  ATLQCLSNTELFAEYLALGQYRAGRPEPSPDPEQPAGRGAQGQGEVTEQLAHLVRALWTLEYTPQHSRDF
  KTIVSKNALQYRGNSQHDAQEFLLWLLDRVHEDLNHSVKQSGQPPLKPPSETDMMPEGPSFPVCSTFVQE
  LFQAQYRSSLTCPHCQKQSNTFDPFLCISLPIPLPHTRPLYVTVVYQGKCSHCMRIGVAVPLSGTVARLR
  EAVSMETKIPTDQIVLTEMYYDGFHRSFCDTDDLETVHESDCIFAFETPEIFRPEGILSQRGIHLNNNLN
  HLKFGLDYHRLSSPTQTAAKQGKMDSPTSRAGSDKIVLLVCNRACTGQQGKRFGLPFVLHLEKTIAWDLL
  QKEILEKMKYFLRPTVCIQVCPFSLRVVSVVGITYLLPQEEQPLCHPIVERALKSCGPGGTAHVKLVVEW
  DKETRDFLFVNTEDEYIPDAESVRLQRERHHQPQTCTLSQCFQLYTKEERLAPDDAWRCPHCKQLQQGSI
  TLSLWTLPDVLIIHLKRFRQEGDRRMKLQNMVKFPLTGLDMTPHVVKRSQSSWSLPSHWSPWRRPYGLGR
  DPEDYIYDLYAVCNHHGTMQGGHYTAYCKNSVDGLWYCFDDSDVQQLSEDEVCTQTAYILFYQRRTAIPS
  WSANSSVAGSTSSSLCEHWVSRLPGSKPASVTSAASSRRTSLASLSESVEMTGERSEDDGGFSTRPFVRS
  VQRQSLSSRSSVTSPLAVNENCMRPSWSLSAKLQMRSNSPSRFSGDSPIHSSASTLEKIGEAADDKVSIS
  CFGSLRNLSSSYQEPSDSHSRREHKAVGRAPLAVMEGVFKDESDTRRLNSSVVDTQSKHSAQGDRLPPLS
  GPFDNNNQIAYVDQSDSVDSSPVKEVKAPSHPGSLAKKPESTTKRSPSSKGTSEPEKSLRKGRPALASQE
  SSLSSTSPSSPLPVKVSLKPSRSRSKADSSSRGSGRHSSPAPAQPKKESSPKSQDSVSSPSPQKQKSASA
  LTYTASSTSAKKASGPATRSPFPPGKSRTSDHSLSREGSRQSLGSDRASATSTSKPNSPRVSQARAGEGR
  GAGKHVRSSSMASLRSPSTSIKSGLKRDSKSEDKGLSFFKSALRQKETRRSTDLGKTALLSKKAGGSSVK
  SVCKNTGDDEAERGHQPPASQQPNANTTGKEQLVTKDPASAKHSLLSARKSKSSQLDSGVPSSPGGRQSA
  EKSSKKLSSSMQTSARPSQKPQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020718
• ORF Size: 4056 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020718.3, NP_065769.3
• RefSeq Size: 10698 bp
• RefSeq ORF: 4059 bp
• Locus ID: 57478
• UniProt ID: Q70CQ4
• Cytogenetics: 16p12.2
• Protein Families: Druggable Genome, Protease
• MW: 146.5 kDa
• Gene Summary: May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).[UniProtKB/Swiss-Prot Function]