JRKL (NM_003772) Human Tagged ORF Clone

CAT#: RC215633

JRKL (Myc-DDK-tagged)-Human jerky homolog-like (mouse) (JRKL)

Product Images

Circular map for RC215633

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: JRKL
• Synonyms: HHMJG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215633 representing NM_003772
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGGAAACGGAAGCGTGTGGTGTTGACTATTAAAGATAAGCTTGATATAATAAAGAAACTTGAAG
  ACGGAGGTTCTTCCAAACAACTGGCAGTGATTTATGGAATTGGTGAAACAACAGTTCGGGATATAAGAAA
  AAATAAGGAAAAGATTATAACTTATGCAAGCAGTTCTGATTCCACAAGTCTTTTGGCCAAGAGGAAATCT
  ATGAAGCCATCCATGTATGAGGAATTGGACAGGGCAATGCTGGAATGGTTCAACCAGCAAAGAGCAAAAG
  GGAATCCCATATCTGGACCAATTTGTGCAAAAAGGGCAGAGTTCTTCTTTTATGCTTTGGGAATGGATGG
  TGATTTTAACCCCTCTGCCGGTTGGCTAACTCGTTTTAAGCAGCGGCACAGCATTAGAGAGATTAACATT
  AGAAATGAAAGATTAAATGGAGATGAGACTGCGGTGGAAGATTTTTGTAATAACTTTCGAGATTTTATTG
  AACGAGAGAATTTACAGCCTGAACAAATCTACAATGCAGATGAAACTGGACTCTTTTGGAAGTGCTTGCC
  TTCTAGGATTTCAGTAATCAAAGGTAAATGCACTGTCCCTGGGCACAAATCAATTGAAGAAAGAGTCACA
  ATCATGTGTTGTGCCAATGCAACAGGTTTACACAAACTTAAACTTTGTGTTGTGGGGAAAGCAAAGAAAC
  CTCGCTCCTTTAAATCAACTGACACCTTAAACCTGCCAGTCTCTTATTTCAGCCAAAAAGGTGCATGGAT
  GGATCTTTCCATTTTCCGACAATGGTTTGATAAAATTTTTGTGCCGCAAGTTCGAGAGTATTTAAGATCT
  AAAGGCTTGCAGGAAAAGGCTGTGCTCTTGTTGGATAATTCACCAACACATCCAAATGAAAATGTCCTAA
  GGTCAGATGATGGCCAAATATTTGCTAAATATTTACCACCTAATGTGGCCTCATTGATTCAGCCTTCAGA
  TCAGGGAGTCATAGCAACGATGAAGAGAAATTATCGTGCAGGTCTTCTCCAGAACAACTTGGAAGAAGGT
  AATGACCTGAAATCATTCTGGAAGAAGCTAACTCTGTTGGATGCACTTTATGAAATAGCAATGGCATGGA
  ACTTAGTAAAACCAGTTACCATTAGCAGAGCATGGAAGAAGATTCTCCCTATGGTAGAGGAGAAAGAGAG
  CCTGGACTTTGATGTTGAAGATATTTCTGTGGCTACTGTGGCTGCCATTTTACAACACACCAAAGGATTG
  GAAAATGTGACTACTGAGAACCTTGAAAAATGGCTTGAAGTAGACAGTACTGAACCAGGCTATGAAGTGT
  TAACTGATAGCGAAATCATCAGAAGAGCACAAGGCCAGGCAGATGAATCCAGTGAAAATGAGGAGGAGGA
  AATAGAACTAATTCCAGAGAAACATATTAATCATGCAGCTGCCCTCCAGTGGACTGAAAATTTATTGGAT
  TATCTAGAACAACAAGGTGATATGATTCTACCTGATAGACTGGTAATACGTAAACTTCGAGCCACCATCA
  GAAATAAACAGAAGATGACAAAGTCAAGTCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215633 representing NM_003772
  Red=Cloning site Green=Tags(s)
  
  MSGKRKRVVLTIKDKLDIIKKLEDGGSSKQLAVIYGIGETTVRDIRKNKEKIITYASSSDSTSLLAKRKS
  MKPSMYEELDRAMLEWFNQQRAKGNPISGPICAKRAEFFFYALGMDGDFNPSAGWLTRFKQRHSIREINI
  RNERLNGDETAVEDFCNNFRDFIERENLQPEQIYNADETGLFWKCLPSRISVIKGKCTVPGHKSIEERVT
  IMCCANATGLHKLKLCVVGKAKKPRSFKSTDTLNLPVSYFSQKGAWMDLSIFRQWFDKIFVPQVREYLRS
  KGLQEKAVLLLDNSPTHPNENVLRSDDGQIFAKYLPPNVASLIQPSDQGVIATMKRNYRAGLLQNNLEEG
  NDLKSFWKKLTLLDALYEIAMAWNLVKPVTISRAWKKILPMVEEKESLDFDVEDISVATVAAILQHTKGL
  ENVTTENLEKWLEVDSTEPGYEVLTDSEIIRRAQGQADESSENEEEEIELIPEKHINHAAALQWTENLLD
  YLEQQGDMILPDRLVIRKLRATIRNKQKMTKSSQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003772
• ORF Size: 1572 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003772.4
• RefSeq Size: 3577 bp
• RefSeq ORF: 1575 bp
• Locus ID: 8690
• UniProt ID: Q9Y4A0
• Cytogenetics: 11q21
• Domains: DDE, CENPB, CENP-B_N
• MW: 59.9 kDa
• Gene Summary: The function of this gene has not yet been defined, however, the encoded protein shares similarity with the human (41% identical) and mouse (34% identical) jerky gene products. This protein may act as a nuclear regulatory protein. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]