CENTG3 (AGAP3) (NM_001042535) Human Tagged ORF Clone

CAT#: RC215506

AGAP3 (Myc-DDK-tagged)-Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 3 (AGAP3), transcript variant 2

Product Images

Circular map for RC215506

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CENTG3
• Synonyms: AGAP-3; CENTG3; cnt-g3; CRAG; MRIP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215506 representing NM_001042535
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTTCCAGGCGGGCGGGGGGCAGAGCCCGCAGCAGCAGCAGAGCCTGGCGGCTCCGGGGGGCGGCG
  GCGCTGCCGCGCAGCAGCTCGTCTGCGGCGGGCAGTTCGGCGGCGCGGGGCCCGGGGCCGGGGGCGGCGG
  CGGCCCCTCGCAGCAGCTGGCCGGCGGGCCCCCCCAGCAGTTCGCGCTCTCCAACTCCGCGGCCATCCGG
  GCCGAGATCCAGCGCTTCGAGTCCGTGCATCCCAATATCTACGCCATCTACGACCTGATCGAGCGCATCG
  AGGATTTGGCGCTGCAGAACCAGATCCGGGAGCACGTCATCTCCATCGAGGACTCGTTTGTGAACAGCCA
  GGAGTGGACGCTGAGCCGCTCCGTACCGGAGCTTAAAGTGGGCATAGTGGGGAACCTGTCTAGCGGGAAG
  TCAGCCCTGGTGCACCGCTATCTGACGGGGACCTATGTCCAGGAGGAGTCCCCTGAAGGGGGGCGGTTTA
  AGAAGGAGATTGTGGTGGATGGCCAGAGTTACCTGCTGCTGATCCGAGATGAAGGAGGCCCCCCTGAGCT
  CCAGTTTGCTGCCTGGGTGGATGCAGTGGTGTTTGTGTTCAGCCTGGAGGATGAAATCAGTTTCCAGACG
  GTGTACAACTACTTCCTGCGTCTCTGCAGCTTCCGCAACGCCAGCGAGGTGCCCATGGTGCTTGTGGGCA
  CGCAGGATGCCATCAGCGCTGCGAATCCCCGGGTTATCGACGACAGCAGAGCCCGCAAGCTCTCCACAGA
  TCTGAAGCGGTGCACCTACTATGAGACGTGCGCGACCTACGGGCTCAATGTGGAGCGTGTCTTCCAGGAC
  GTGGCCCAGAAGGTAGTGGCCTTGCGAAAGAAGCAGCAACTGGCCATCGGGCCCTGCAAGTCACTGCCCA
  ACTCGCCCAGCCACTCGGCCGTGTCCGCCGCCTCCATCCCGGCCGTGCACATCAACCAGGCCACGAATGG
  CGGCGGCAGCGCCTTCAGCGACTACTCGTCCTCAGTCCCCTCCACCCCCAGCATCAGCCAGCGGGAGCTG
  CGCATCGAGACCATCGCTGCCTCCTCCACCCCCACACCCATCCGAAAGCAGTCCAAGCGGCGCTCCAACA
  TCTTCACGATATGTGCCACTGTTTCCAACTTTTCATCAACAAAAAGGCCTTTCCAACTCCTTCCAAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215506 representing NM_001042535
  Red=Cloning site Green=Tags(s)
  
  MNFQAGGGQSPQQQQSLAAPGGGGAAAQQLVCGGQFGGAGPGAGGGGGPSQQLAGGPPQQFALSNSAAIR
  AEIQRFESVHPNIYAIYDLIERIEDLALQNQIREHVISIEDSFVNSQEWTLSRSVPELKVGIVGNLSSGK
  SALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPELQFAAWVDAVVFVFSLEDEISFQT
  VYNYFLRLCSFRNASEVPMVLVGTQDAISAANPRVIDDSRARKLSTDLKRCTYYETCATYGLNVERVFQD
  VAQKVVALRKKQQLAIGPCKSLPNSPSHSAVSAASIPAVHINQATNGGGSAFSDYSSSVPSTPSISQREL
  RIETIAASSTPTPIRKQSKRRSNIFTICATVSNFSSTKRPFQLLPN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042535
• ORF Size: 1188 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042535.4
• RefSeq Size: 2063 bp
• RefSeq ORF: 1191 bp
• Locus ID: 116988
• UniProt ID: Q96P47
• Cytogenetics: 7q36.1
• MW: 42.3 kDa
• Gene Summary: This gene encodes an essential component of the N-methyl-D-aspartate (NMDA) receptor signaling complex which mediates long-term potentiation in synapses by linking activation of NMDA receptor to alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor trafficking. The encoded protein contains an N-terminal GTPase-like domain, a pleckstrin homology domain, an ArfGAP domain and several C-terminal ankryn repeat domains. [provided by RefSeq, Apr 2017]