Desmuslin (SYNM) (NM_145728) Human Tagged ORF Clone

CAT#: RC215288

SYNM (Myc-DDK-tagged)-Human synemin, intermediate filament protein (SYNM), transcript variant A

Product Images

Circular map for RC215288

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SYNM (Cat# RC215288, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SYNM(Cat# TA806550). Positive lysates LY407863 (100ug) and LC407863 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY407863) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215288 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Desmuslin
• Synonyms: DMN; SYN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215288 representing NM_145728
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGTCCTGGCGGCTGCAGACGGGCCCCGAGAAGGCCGAGCTCCAGGAGCTCAACGCCCGGCTCTATG
  ACTACGTGTGTCGGGTGCGGGAGCTGGAGCGCGAAAACCTACTCCTGGAGGAGGAGCTGCGCGGCCGGCG
  CGGGCGAGAGGGCCTGTGGGCCGAGGGGCAGGCCCGCTGCGCCGAGGAGGCGCGCAGCTTGCGGCAGCAG
  CTGGACGAGCTGAGCTGGGCCACTGCGCTGGCGGAGGGCGAGCGGGACGCTCTGCGGCGCGAGCTGCGGG
  AGCTGCAGCGCCTGGATGCGGAGGAGCGCGCCGCCCGCGGCCGCCTGGACGCCGAGCTGGGTGCGCAGCA
  GCGCGAGCTGCAGGAGGCGCTGGGCGCGCGCGCCGCCCTCGAGGCGCTGCTGGGCCGGCTGCAGGCCGAG
  CGCCGAGGCCTCGACGCGGCCCACGAACGCGACGTGAGGGAGCTGCGCGCGCGCGCCGCCAGCCTTACCA
  TGCATTTCCGCGCCCGCGCCACCGGCCCCGCCGCGCCGCCGCCACGCCTGCGGGAGGTGCACGACAGCTA
  CGCACTGCTGGTGGCCGAGTCGTGGCGGGAGACGGTGCAGCTGTACGAGGACGAGGTGCGCGAGCTGGAG
  GAGGCGCTGCGGCGCGGCCAGGAGAGCAGACTCCAGGCGGAGGAAGAGACGCGGCTGTGCGCGCAGGAGG
  CAGAGGCGCTGCGGCGCGAGGCGCTCGGGTTGGAGCAGCTGCGCGCGCGGCTGGAGGACGCGCTGCTGCG
  GATGCGCGAGGAGTACGGGATACAGGCCGAGGAGCGGCAGAGAGCGATTGACTGCCTGGAGGATGAGAAG
  GCAACCCTCACCTTGGCCATGGCTGACTGGCTGCGGGACTATCAGGACCTCCTGCAGGTGAAGACCGGCC
  TCAGTCTGGAGGTGGCGACCTACCGGGCCTTATTGGAAGGAGAAAGTAATCCAGAGATAGTGATCTGGGC
  TGAGCACGTTGAAAACATGCCGTCAGAATTCAGAAACAAATCCTATCACTATACCGACTCACTACTACAG
  AGGGAAAATGAATGGAATCTATTTTCAAGGCAGAAAGCACCTTTGGCAAGTTTCAATCACAGCTCGGCAC
  TGTATTCTAACCTGTCAGGGCACCGTGGATCTCAGACGGGCACATCTATTGGAGGTGATGCCAGAAGAGG
  CTTCTTGGGCTCGGGATATTCTTCCTCGGCCACTACCCAGCAGGAAAACTCATACGGAAAAGCCGTCAGC
  AGTCAAACCAACGTCAGAACTTTCTCTCCAACCTATGGCCTTTTAAGAAATACTGAGGCTCAAGTGAAAA
  CATTCCCTGACAGACCAAAAGCCGGAGATACAAGGGAGGTCCCCGTTTACATAGGTGAAGATTCCACAAT
  TGCCCGCGAGTCGTACCGGGATCGCCGAGACAAGGTGGCAGCAGGTGCTTCGGAAAGCACACGGTCAAAT
  GAGAGGACCGTCATTCTGGGAAAGAAAACAGAAGTGAAAGCCACGAGGGAGCAAGAAAGAAACAGACCAG
  AAACCATCCGAACAAAGCCAGAAGAGAAAATGTTCGATTCTAAAGAGAAGGCTTCTGAGGAGAGAAACCT
  AAGATGGGAAGAATTGACAAAGTTAGATAAGGAAGCGAGACAGAGAGAAAGCCAGCAGATGAAGGAGAAG
  GCTAAGGAGAAGGACTCACCGAAGGAGAAGAGTGTGCGAGAGAGAGAGGTGCCGATTAGTCTAGAAGTAT
  CCCAGGACAGAAGAGCAGAGGTGTCCCCGAAAGGTTTGCAGACGCCTGTGAAGGATGCTGGTGGTGGGAC
  CGGTAGAGAGGCAGAAGCAAGAGAGCTACGGTTCAGGTTGGGCACCAGTGATGCCACTGGTTCTCTGCAA
  GGCGATTCCATGACAGAAACCGTAGCAGAAAACATCGTTACCAGTATCCTGAAGCAGTTCACTCAGTCTC
  CAGAGACAGAAGCATCTGCTGATTCTTTTCCAGACACAAAAGTCACTTACGTGGACAGGAAAGAGCTTCC
  TGGGGAAAGGAAAACAAAGACTGAAATAGTTGTGGAGTCTAAACTGACTGAGGATGTTGATGTTTCCGAT
  GAAGCTGGCCTGGACTACCTTTTAAGCAAGGATATTAAGGAAGTGGGGCTGAAAGGCAAGTCAGCCGAGC
  AGATGATAGGAGACATCATCAACCTCGGCCTGAAAGGGAGGGAGGGGAGAGCAAAGGTCGTCAACGTGGA
  GATCGTGGAGGAGCCCGTGAGTTATGTCAGCGGGGAGAAGCCGGAGGAGTTTTCCGTCCCATTCAAAGTG
  GAGGAGGTCGAAGATGTGTCGCCAGGCCCCTGGGGGTTGGTTAAGGAGGAGGAAGGTTATGGAGAAAGCG
  ATGTCACATTCTCAGTTAATCAGCATCGAAGGACCAAGCAGCCCCAGGAGAACACGACTCACGTGGAAGA
  AGTGACAGAGGCAGGTGATTCAGAGGGCGAGCAGAGTTATTTTGTGTCCACTCCAGATGAACACCCCGGG
  GGGCACGACAGAGATGACGGCTCGGTGTACGGGCAGATCCACATCGAGGAGGAATCCACCATCAGGTACT
  CTTGGCAGGATGAAATCGTGCAGGGGACTCGAAGGAGGACACAGAAGGACGGTGCAGTGGGCGAGAAGGT
  TGTGAAGCCCTTGGATGTCCCAGCGCCCTCTCTGGAGGGGGACCTGGGTTCCACTCACTGGAAAGAACAA
  GCTAGAAGCGGTGAATTTCATGCCGAACCCACAGTCATTGAAAAAGAAATTAAAATACCCCACGAATTCC
  ACACCTCCATGAAGGGCATCTCCTCCAAGGAGCCCCGGCAGCAGCTGGTGGAGGTCATCGGGCAGCTGGA
  GGAAACCCTTCCCGAGCGCATGAGGGAGGAGCTGTCCGCCCTCACCAGAGAGGGGCAGGGTGGGCCGGGG
  AGCGTTTCCGTGGATGTCAAGAAGGTCCAGGGTGCTGGTGGCAGTTCCGTGACCCTGGTTGCTGAAGTCA
  ACGTCTCACAAACTGTGGATGCCGATCGGTTAGACCTGGAGGAGCTGAGCAAAGATGAGGCCAGTGAGAT
  GGAGAAGGCTGTGGAGTCGGTGGTTCGGGAGAGCCTGAGCAGGCAACGCAGCCCAGCGCCTGGCAGCCCA
  GATGAGGAAGGTGGAGCGGAGGCCCCGGCTGCTGGCATTCGCTTTAGGCGTTGGGCCACCCGGGAGCTGT
  ACATCCCTTCAGGCGAGAGCGAGGTTGCTGGTGGGGCCTCTCACAGCTCGGGACAGCGCACTCCCCAGGG
  CCCAGTGTCGGCCACTGTGGAGGTCAGCAGCCCCACAGGCTTTGCCCAGTCACAGGTGCTGGAGGATGTG
  AGCCAGGCTGCAAGGCACATAAAACTCGGCCCCTCTGAAGTCTGGAGGACTGAGCGAATGTCATATGAAG
  GACCCACTGCAGAAGTGGTGGAGGTAAGTGCGGGAGGTGACCTAAGTCAGGCAGCGAGCCCGACCGGAGC
  CAGCCGGTCTGTGAGGCATGTCACGCTGGGTCCCGGTCAAAGTCCACTGTCCAGAGAAGTCATCTTCCTA
  GGCCCTGCCCCTGCCTGTCCAGAGGCATGGGGCTCGCCAGAACCTGGCCCAGCAGAGTCTTCTGCAGATA
  TGGACGGATCAGGGAGGCACAGCACATTTGGCTGCAGACAATTTCATGCTGAAAAGGAGATTATTTTTCA
  GGGCCCCATTTCTGCTGCAGGGAAGGTTGGTGATTATTTTGCAACAGAAGAGTCAGTGGGTACCCAGACT
  TCTGTCAGGCAACTCCAGTTAGGCCCTAAAGAAGGGTTCAGTGGGCAAATCCAGTTCACAGCTCCACTTT
  CAGACAAGGTGGAGTTGGGTGTCATAGGAGATTCTGTACACATGGAAGGGTTGCCAGGGAGCAGCACATC
  CATCAGGCACATCAGCATTGGGCCTCAGAGGCATCAGACCACCCAGCAGATAGTTTACCATGGGCTGGTT
  CCCCAACTGGGGGAATCTGGTGACTCAGAGAGCACTGTGCACGGAGAGGGCTCAGCAGATGTGCACCAGG
  CCACTCACAGTCATACCTCGGGTAGACAAACCGTTATGACTGAAAAGAGCACCTTCCAAAGTGTCGTTTC
  TGAATCTCCCCAGGAGGATAGTGCAGGGGACACATCAGGGGCAGAAATGACATCGGGTGTTAGCAGATCC
  TTTAGGCACATTCGACTAGGTCCTACAGAAACGGAAACCTCTGAACACATTGCCATCCGTGGACCCGTGT
  CCAGAACATTTGTGCTTGCTGGTTCAGCGGACTCCCCTGAGCTAGGCAAGTTAGCAGACAGCAGCAGAAC
  GCTAAGGCACATTGCACCAGGGCCCAAAGAAACTTCGTTTACCTTTCAGATGGATGTGAGTAACGTAGAG
  GCGATCCGCAGCCGGACACAGGAAGCGGGAGCTCTCGGTGTGTCTGACCGTGGTTCCTGGAGAGACGCGG
  ACAGTAGGAATGACCAGGCAGTTGGTGTGAGCTTTAAGGCCTCTGCTGGGGAAGGAGACCAGGCCCACAG
  AGAACAGGGCAAGGAGCAGGCCATGTTTGATAAGAAGGTGCAGCTCCAGAGAATGGTAGACCAAAGGTCG
  GTGATTTCAGATGAAAAGAAAGTTGCCCTCCTCTATCTAGACAATGAGGAGGAGGAGAATGATGGGCATT
  GGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215288 representing NM_145728
  Red=Cloning site Green=Tags(s)
  
  MLSWRLQTGPEKAELQELNARLYDYVCRVRELERENLLLEEELRGRRGREGLWAEGQARCAEEARSLRQQ
  LDELSWATALAEGERDALRRELRELQRLDAEERAARGRLDAELGAQQRELQEALGARAALEALLGRLQAE
  RRGLDAAHERDVRELRARAASLTMHFRARATGPAAPPPRLREVHDSYALLVAESWRETVQLYEDEVRELE
  EALRRGQESRLQAEEETRLCAQEAEALRREALGLEQLRARLEDALLRMREEYGIQAEERQRAIDCLEDEK
  ATLTLAMADWLRDYQDLLQVKTGLSLEVATYRALLEGESNPEIVIWAEHVENMPSEFRNKSYHYTDSLLQ
  RENEWNLFSRQKAPLASFNHSSALYSNLSGHRGSQTGTSIGGDARRGFLGSGYSSSATTQQENSYGKAVS
  SQTNVRTFSPTYGLLRNTEAQVKTFPDRPKAGDTREVPVYIGEDSTIARESYRDRRDKVAAGASESTRSN
  ERTVILGKKTEVKATREQERNRPETIRTKPEEKMFDSKEKASEERNLRWEELTKLDKEARQRESQQMKEK
  AKEKDSPKEKSVREREVPISLEVSQDRRAEVSPKGLQTPVKDAGGGTGREAEARELRFRLGTSDATGSLQ
  GDSMTETVAENIVTSILKQFTQSPETEASADSFPDTKVTYVDRKELPGERKTKTEIVVESKLTEDVDVSD
  EAGLDYLLSKDIKEVGLKGKSAEQMIGDIINLGLKGREGRAKVVNVEIVEEPVSYVSGEKPEEFSVPFKV
  EEVEDVSPGPWGLVKEEEGYGESDVTFSVNQHRRTKQPQENTTHVEEVTEAGDSEGEQSYFVSTPDEHPG
  GHDRDDGSVYGQIHIEEESTIRYSWQDEIVQGTRRRTQKDGAVGEKVVKPLDVPAPSLEGDLGSTHWKEQ
  ARSGEFHAEPTVIEKEIKIPHEFHTSMKGISSKEPRQQLVEVIGQLEETLPERMREELSALTREGQGGPG
  SVSVDVKKVQGAGGSSVTLVAEVNVSQTVDADRLDLEELSKDEASEMEKAVESVVRESLSRQRSPAPGSP
  DEEGGAEAPAAGIRFRRWATRELYIPSGESEVAGGASHSSGQRTPQGPVSATVEVSSPTGFAQSQVLEDV
  SQAARHIKLGPSEVWRTERMSYEGPTAEVVEVSAGGDLSQAASPTGASRSVRHVTLGPGQSPLSREVIFL
  GPAPACPEAWGSPEPGPAESSADMDGSGRHSTFGCRQFHAEKEIIFQGPISAAGKVGDYFATEESVGTQT
  SVRQLQLGPKEGFSGQIQFTAPLSDKVELGVIGDSVHMEGLPGSSTSIRHISIGPQRHQTTQQIVYHGLV
  PQLGESGDSESTVHGEGSADVHQATHSHTSGRQTVMTEKSTFQSVVSESPQEDSAGDTSGAEMTSGVSRS
  FRHIRLGPTETETSEHIAIRGPVSRTFVLAGSADSPELGKLADSSRTLRHIAPGPKETSFTFQMDVSNVE
  AIRSRTQEAGALGVSDRGSWRDADSRNDQAVGVSFKASAGEGDQAHREQGKEQAMFDKKVQLQRMVDQRS
  VISDEKKVALLYLDNEEEENDGHWF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145728
• ORF Size: 4695 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145728.2
• RefSeq Size: 7343 bp
• RefSeq ORF: 4698 bp
• Locus ID: 23336
• UniProt ID: O15061
• Cytogenetics: 15q26.3
• MW: 172.7 kDa
• Gene Summary: The protein encoded by this gene is an intermediate filament (IF) family member. IF proteins are cytoskeletal proteins that confer resistance to mechanical stress and are encoded by a dispersed multigene family. This protein has been found to form a linkage between desmin, which is a subunit of the IF network, and the extracellular matrix, and provides an important structural support in muscle. Two alternatively spliced variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]