TLR1 (NM_003263) Human Tagged ORF Clone

CAT#: RC215218

TLR1 (Myc-DDK-tagged)-Human toll-like receptor 1 (TLR1)

Product Images

Circular map for RC215218

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TLR1
• Synonyms: CD281; rsc786; TIL; TIL. LPRS5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215218 representing NM_003263
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTAGCATCTTCCATTTTGCCATTATCTTCATGTTAATACTTCAGATCAGAATACAATTATCTGAAG
  AAAGTGAATTTTTAGTTGATAGGTCAAAAAACGGTCTCATCCACGTTCCTAAAGACCTATCCCAGAAAAC
  AACAATCTTAAATATATCGCAAAATTATATATCTGAGCTTTGGACTTCTGACATCTTATCACTGTCAAAA
  CTGAGGATTTTGATAATTTCTCATAATAGAATCCAGTATCTTGATATCAGTGTTTTCAAATTCAACCAGG
  AATTGGAATACTTGGATTTGTCCCACAACAAGTTGGTGAAGATTTCTTGCCACCCTACTGTGAACCTCAA
  GCACTTGGACCTGTCATTTAATGCATTTGATGCCCTGCCTATATGCAAAGAGTTTGGCAATATGTCTCAA
  CTAAAATTTCTGGGGTTGAGCACCACACACTTAGAAAAATCTAGTGTGCTGCCAATTGCTCATTTGAATA
  TCAGCAAGGTCTTGCTGGTCTTAGGAGAGACTTATGGGGAAAAAGAAGACCCTGAGGGCCTTCAAGACTT
  TAACACTGAGAGTCTGCACATTGTGTTCCCCACAAACAAAGAATTCCATTTTATTTTGGATGTGTCAGTC
  AAGACTGTAGCAAATCTGGAACTATCTAATATCAAATGTGTGCTAGAAGATAACAAATGTTCTTACTTCC
  TAAGTATTCTGGCGAAACTTCAAACAAATCCAAAGTTATCAAATCTTACCTTAAACAACATTGAAACAAC
  TTGGAATTCTTTCATTAGGATCCTCCAGCTGGTTTGGCATACAACTGTATGGTATTTCTCAATTTCAAAC
  GTGAAGCTACAGGGTCAGCTGGACTTCAGAGATTTTGATTATTCTGGCACTTCCTTGAAGGCCTTGTCTA
  TACACCAAGTTGTCAGCGATGTGTTCGGTTTTCCGCAAAGTTATATCTATGAAATCTTTTCGAATATGAA
  CATCAAAAATTTCACAGTGTCTGGTACACGCATGGTCCACATGCTTTGCCCATCCAAAATTAGCCCGTTC
  CTGCATTTGGATTTTTCCAATAATCTCTTAACAGACACGGTTTTTGAAAATTGTGGGCACCTTACTGAGT
  TGGAGACACTTATTTTACAAATGAATCAATTAAAAGAACTTTCAAAAATAGCTGAAATGACTACACAGAT
  GAAGTCTCTGCAACAATTGGATATTAGCCAGAATTCTGTAAGCTATGATGAAAAGAAAGGAGACTGTTCT
  TGGACTAAAAGTTTATTAAGTTTAAATATGTCTTCAAATATACTTACTGACACTATTTTCAGATGTTTAC
  CTCCCAGGATCAAGGTACTTGATCTTCACAGCAATAAAATAAAGAGCATTCCTAAACAAGTCGTAAAACT
  GGAAGCTTTGCAAGAACTCAATGTTGCTTTCAATTCTTTAACTGACCTTCCTGGATGTGGCAGCTTTAGC
  AGCCTTTCTGTATTGATCATTGATCACAATTCAGTTTCCCACCCATCGGCTGATTTCTTCCAGAGCTGCC
  AGAAGATGAGGTCAATAAAAGCAGGGGACAATCCATTCCAATGTACCTGTGAGCTAGGAGAATTTGTCAA
  AAATATAGACCAAGTATCAAGTGAAGTGTTAGAGGGCTGGCCTGATTCTTATAAGTGTGACTACCCGGAA
  AGTTATAGAGGAACCCTACTAAAGGACTTTCACATGTCTGAATTATCCTGCAACATAACTCTGCTGATCG
  TCACCATCGTTGCCACCATGCTGGTGTTGGCTGTGACTGTGACCTCCCTCTGCAGCTACTTGGATCTGCC
  CTGGTATCTCAGGATGGTGTGCCAGTGGACCCAGACCCGGCGCAGGGCCAGGAACATACCCTTAGAAGAA
  CTCCAAAGAAATCTCCAGTTTCATGCATTTATTTCATATAGTGGGCACGATTCTTTCTGGGTGAAGAATG
  AATTATTGCCAAACCTAGAGAAAGAAGGTATGCAGATTTGCCTTCATGAGAGAAACTTTGTTCCTGGCAA
  GAGCATTGTGGAAAATATCATCACCTGCATTGAGAAGAGTTACAAGTCCATCTTTGTTTTGTCTCCCAAC
  TTTGTCCAGAGTGAATGGTGCCATTATGAACTCTACTTTGCCCATCACAATCTCTTTCATGAAGGATCTA
  ATAGCTTAATCCTGATCTTGCTGGAACCCATTCCTCAGTACTCCATTCCTAGCAGTTATCACAAGCTCAA
  AAGTCTCATGGCCAGGAGGACTTATTTGGAATGGCCCAAGGAAAAGAGCAAACGTGGCCTTTTTTGGGCT
  AACTTAAGGGCAGCCATTAATATTAAGCTGACAGAGCAAGCAAAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215218 representing NM_003263
  Red=Cloning site Green=Tags(s)
  
  MTSIFHFAIIFMLILQIRIQLSEESEFLVDRSKNGLIHVPKDLSQKTTILNISQNYISELWTSDILSLSK
  LRILIISHNRIQYLDISVFKFNQELEYLDLSHNKLVKISCHPTVNLKHLDLSFNAFDALPICKEFGNMSQ
  LKFLGLSTTHLEKSSVLPIAHLNISKVLLVLGETYGEKEDPEGLQDFNTESLHIVFPTNKEFHFILDVSV
  KTVANLELSNIKCVLEDNKCSYFLSILAKLQTNPKLSNLTLNNIETTWNSFIRILQLVWHTTVWYFSISN
  VKLQGQLDFRDFDYSGTSLKALSIHQVVSDVFGFPQSYIYEIFSNMNIKNFTVSGTRMVHMLCPSKISPF
  LHLDFSNNLLTDTVFENCGHLTELETLILQMNQLKELSKIAEMTTQMKSLQQLDISQNSVSYDEKKGDCS
  WTKSLLSLNMSSNILTDTIFRCLPPRIKVLDLHSNKIKSIPKQVVKLEALQELNVAFNSLTDLPGCGSFS
  SLSVLIIDHNSVSHPSADFFQSCQKMRSIKAGDNPFQCTCELGEFVKNIDQVSSEVLEGWPDSYKCDYPE
  SYRGTLLKDFHMSELSCNITLLIVTIVATMLVLAVTVTSLCSYLDLPWYLRMVCQWTQTRRRARNIPLEE
  LQRNLQFHAFISYSGHDSFWVKNELLPNLEKEGMQICLHERNFVPGKSIVENIITCIEKSYKSIFVLSPN
  FVQSEWCHYELYFAHHNLFHEGSNSLILILLEPIPQYSIPSSYHKLKSLMARRTYLEWPKEKSKRGLFWA
  NLRAAINIKLTEQAKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003263
• ORF Size: 2358 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003263.4
• RefSeq Size: 2867 bp
• RefSeq ORF: 2361 bp
• Locus ID: 7096
• UniProt ID: Q15399
• Cytogenetics: 4p14
• Domains: TIR, LRRCT, LRR, LRR_TYP
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Toll-like receptor signaling pathway
• MW: 90.1 kDa
• Gene Summary: The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene. [provided by RefSeq, Jul 2008]