L3MBTL3 (NM_032438) Human Tagged ORF Clone

CAT#: RC215097

L3MBTL3 (Myc-DDK-tagged)-Human l(3)mbt-like 3 (Drosophila) (L3MBTL3), transcript variant 1

Product Images

Circular map for RC215097

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L3MBTL3
• Synonyms: MBT-1; MBT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215097 representing NM_032438
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGAATCTGCCTCTAGCACAAGTGGTCAAGAGTTTGATGTGTTCAGTGTTATGGACTGGAAAGATG
  GAGTGGGCACGTTACCAGGAAGTGACTTAAAGTTTCGGGTAAATGAGTTTGGAGCCCTGGAAGTTATTAC
  AGATGAGAATGAGATGGAAAATGTTAAAAAAGCAACTGCTACCACCACTTGGATGGTACCAACTGCTCAA
  GAAGCCCCGACCTCTCCCCCGAGCTCCAGGCCCGTATTTCCACCTGCCTACTGGACATCTCCACCTGGAT
  GTCCCACAGTCTTTTCAGAGAAGACTGGGATGCCTTTCAGGTTGAAGGATCCAGTGAAAGTAGAAGGGCT
  TCAGTTCTGTGAGAACTGTTGTCAGTATGGCAACGTAGATGAGTGTCTTTCTGGAGGAAACTATTGCAGC
  CAGAATTGTGCTCGGCACATCAAAGATAAAGATCAGAAGGAAGAAAGGGACGTAGAAGAAGACAATGAGG
  AAGAAGATCCTAAGTGTAGTCGGAAGAAAAAACCAAAATTATCTCTGAAAGCTGACACCAAGGAGGATGG
  AGAAGAGAGAGATGATGAAATGGAGAACAAACAAGATGTAAGAATCCTGAGGGGTTCGCAGAGAGCACGG
  AGGAAAAGACGAGGGGATTCGGCTGTACTAAAGCAGGGTTTGCCTCCTAAAGGAAAGAAAGCGTGGTGCT
  GGGCATCCTACCTGGAAGAGGAGAAAGCGGTGGCAGTGCCGGCGAAGCTGTTCAAGGAGCATCAATCCTT
  TCCATATAACAAAAATGGATTCAAAGTTGGCATGAAATTAGAAGGCGTGGATCCTGAGCATCAGTCTGTG
  TACTGTGTCCTCACCGTCGCGGAGGTTTGTGGATACCGGATAAAGCTTCACTTTGATGGGTATTCTGATT
  GCTATGACTTCTGGGTGAATGCAGACGCTCTGGATATCCACCCAGTTGGGTGGTGTGAGAAAACCGGCCA
  CAAACTCCATCCTCCAAAAGGGTATAAAGAAGAAGAATTCAATTGGCAGACCTATCTTAAGACATGTAAA
  GCTCAAGCTGCTCCTAAGTCATTATTTGAAAATCAGAATATAACAGTGATCCCATCGGGCTTTCGAGTTG
  GTATGAAGCTTGAGGCAGTAGACAAAAAGAATCCCTCATTCATCTGTGTTGCTACGGTAACAGATATGGT
  GGACAATCGTTTCCTGGTACATTTTGACAACTGGGATGAGAGCTATGACTATTGGTGTGAAGCATCAAGT
  CCACATATTCATCCAGTTGGTTGGTGTAAGGAACATAGAAGAACCCTTATTACTCCACCAGGTTATCCAA
  ATGTGAAACATTTTTCTTGGGATAAATACTTAGAAGAAACCAATTCTTTACCTGCTCCTGCAAGAGCTTT
  CAAAGTGAAACCTCCTCATGGATTCCAGAAAAAAATGAAGCTTGAGGTTGTAGACAAAAGGAACCCTATG
  TTTATTAGAGTAGCAACTGTGGCAGACACAGATGATCACCGGGTAAAAGTTCACTTTGATGGCTGGAACA
  ATTGCTATGATTACTGGATAGATGCAGATTCTCCTGATATTCACCCTGTAGGCTGGTGTTCAAAAACAGG
  ACATCCCCTTCAGCCTCCTTTGAGCCCCTTAGAATTAATGGAAGCTTCAGAACATGGTGGATGCTCAACC
  CCGGGATGTAAAGGGATTGGCCATTTCAAGAGAGCGAGACATCTGGGCCCTCACAGTGCTGCCAACTGTC
  CCTATTCAGAAATCAATTTGAATAAAGACCGTATTTTTCCAGACCGCTTAAGTGGTGAGATGCCTCCGGC
  TAGTCCGTCATTTCCAAGAAATAAAAGGACAGATGCAAATGAAAGCTCTTCTTCCCCTGAAATCAGAGAC
  CAGCATGCTGATGATGTCAAAGAAGACTTTGAAGAGAGAACAGAAAGTGAAATGAGAACATCACATGAAG
  CCAGAGGTGCCCGGGAAGAACCCACCGTCCAGCAGGCACAGCGTCGGTCAGCTGTCTTTCTGTCCTTTAA
  GTCCCCAATTCCATGTCTGCCCTTGCGCTGGGAGCAGCAAAGCAAACTTCTTCCAACTGTCGCAGGAATC
  CCTGCCAGTAAAGTTTCCAAATGGAGCACAGACGAGGTGTCAGAATTTATACAGAGCTTACCTGGGTGTG
  AAGAACATGGAAAGGTATTTAAAGATGAACAAATTGATGGAGAAGCATTTCTACTTATGACTCAAACAGA
  CATTGTTAAAATTATGAGCATTAAACTGGGCCCTGCTCTCAAAATTTTCAATTCCATCCTGATGTTCAAA
  GCTGCAGAGAAGAATTCTCACAATGAACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215097 representing NM_032438
  Red=Cloning site Green=Tags(s)
  
  MTESASSTSGQEFDVFSVMDWKDGVGTLPGSDLKFRVNEFGALEVITDENEMENVKKATATTTWMVPTAQ
  EAPTSPPSSRPVFPPAYWTSPPGCPTVFSEKTGMPFRLKDPVKVEGLQFCENCCQYGNVDECLSGGNYCS
  QNCARHIKDKDQKEERDVEEDNEEEDPKCSRKKKPKLSLKADTKEDGEERDDEMENKQDVRILRGSQRAR
  RKRRGDSAVLKQGLPPKGKKAWCWASYLEEEKAVAVPAKLFKEHQSFPYNKNGFKVGMKLEGVDPEHQSV
  YCVLTVAEVCGYRIKLHFDGYSDCYDFWVNADALDIHPVGWCEKTGHKLHPPKGYKEEEFNWQTYLKTCK
  AQAAPKSLFENQNITVIPSGFRVGMKLEAVDKKNPSFICVATVTDMVDNRFLVHFDNWDESYDYWCEASS
  PHIHPVGWCKEHRRTLITPPGYPNVKHFSWDKYLEETNSLPAPARAFKVKPPHGFQKKMKLEVVDKRNPM
  FIRVATVADTDDHRVKVHFDGWNNCYDYWIDADSPDIHPVGWCSKTGHPLQPPLSPLELMEASEHGGCST
  PGCKGIGHFKRARHLGPHSAANCPYSEINLNKDRIFPDRLSGEMPPASPSFPRNKRTDANESSSSPEIRD
  QHADDVKEDFEERTESEMRTSHEARGAREEPTVQQAQRRSAVFLSFKSPIPCLPLRWEQQSKLLPTVAGI
  PASKVSKWSTDEVSEFIQSLPGCEEHGKVFKDEQIDGEAFLLMTQTDIVKIMSIKLGPALKIFNSILMFK
  AAEKNSHNEL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032438
• ORF Size: 2340 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032438.4
• RefSeq Size: 4217 bp
• RefSeq ORF: 2343 bp
• Locus ID: 84456
• UniProt ID: Q96JM7
• Cytogenetics: 6q23.1
• MW: 88.3 kDa
• Gene Summary: This gene encodes a member of the malignant brain tumor (MBT) family of chromatin interacting transcriptional repressors. Members of this family function as methyl-lysine readers, which recognize methylated lysine residues on histone protein tails, and are associated with the repression of gene expression. The encoded protein may regulate hematopoiesis. Homozygous deletion of this gene has been observed in human patients with medulloblastoma. [provided by RefSeq, Oct 2016]