BMP8A (NM_181809) Human Tagged ORF Clone

CAT#: RC215047

BMP8A (Myc-DDK-tagged)-Human bone morphogenetic protein 8a (BMP8A)

Product Images

Circular map for RC215047

• 

  Western blot validation of overexpression lysate (Cat# LY405600) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215047 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BMP8A
• Synonyms: OP-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215047 representing NM_181809
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCGCGCCCCGGACCGCTCTGGCTTCTGGGCCTGACGTTGTGCGCGCTGGGCGGGGGCGGCCCCG
  GCCTGCGACCCCCGCCCGGCTGTCCCCAGCGACGTCTGGGCGCGCGCGAGCGCCGGGACGTGCAGCGCGA
  GATCCTGGCGGTGCTCGGGCTACCCGGGCGGCCCCGGCCCCGCGCGCCACCCGCCGCCTCCCGGCTGCCC
  GCGTCCGCGCCGCTCTTCATGCTGGACCTGTACCACGCCATGGCTGGCGACGACGACGAGGACGGCGCGC
  CCGCGGAGCAGCGCCTGGGCCGCGCCGACCTGGTCATGAGCTTCGTCAACATGGTGGAGCGAGACCGTGC
  CCTGGGCCACCAGGAGCCCCATTGGAAGGAGTTCCGCTTTGACCTGACCCAGATCCCGGCTGGGGAGGCG
  GTCACAGCTGCGGAGTTCCGGATTTACAAGGTGCCCAGCATCCACCTGCTCAACAGGACCCTCCACGTCA
  GCATGTTCCAGGTGGTCCAGGAGCAGTCCAACAGGGAGTCTGACTTGTTCTTTTTGGATCTTCAGACGCT
  CCGAGCTGGAGACGAGGGCTGGCTGGTGCTGGATGTCACAGCAGCCAGTGACTGCTGGTTGCTGAAGCGT
  CACAAGGACCTGGGACTCCGCCTCTATGTGGAGACTGAGGATGGGCACAGCGTGGATCCTGGCCTGGCCG
  GCCTGCTGGGTCAACGGGCCCCACGCTCCCAACAGCCTTTCGTGGTCACTTTCTTCAGGGCCAGTCCGAG
  TCCCATCCGCACCCCTCGGGCAGTGAGGCCACTGAGGAGGAGGCAGCCGAAGAAAACCAACGAGCTGCCG
  CAGGCCAACCGACTCCCAGGGATCTTTGATGACGTCCACGGCTCCCACGGCCGGCAGGTCTGCCGTCGGC
  ACGAGCTCTACGTCAGCTTCCAGGACCTTGGCTGGCTGGACTGGGTCATCGCCCCCCAAGGCTACTCAGC
  CTATTACTGTGAGGGGGAGTGCTCCTTCCCGCTGGACTCCTGCATGAACGCCACCAACCACGCCATCCTG
  CAGTCCCTGGTGCACCTGATGAAGCCAAACGCAGTCCCCAAGGCGTGCTGTGCACCCACCAAGCTGAGCG
  CCACCTCTGTGCTCTACTATGACAGCAGCAACAACGTCATCCTGCGCAAGCACCGCAACATGGTGGTCAA
  GGCCTGCGGCTGCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215047 representing NM_181809
  Red=Cloning site Green=Tags(s)
  
  MAARPGPLWLLGLTLCALGGGGPGLRPPPGCPQRRLGARERRDVQREILAVLGLPGRPRPRAPPAASRLP
  ASAPLFMLDLYHAMAGDDDEDGAPAEQRLGRADLVMSFVNMVERDRALGHQEPHWKEFRFDLTQIPAGEA
  VTAAEFRIYKVPSIHLLNRTLHVSMFQVVQEQSNRESDLFFLDLQTLRAGDEGWLVLDVTAASDCWLLKR
  HKDLGLRLYVETEDGHSVDPGLAGLLGQRAPRSQQPFVVTFFRASPSPIRTPRAVRPLRRRQPKKTNELP
  QANRLPGIFDDVHGSHGRQVCRRHELYVSFQDLGWLDWVIAPQGYSAYYCEGECSFPLDSCMNATNHAIL
  QSLVHLMKPNAVPKACCAPTKLSATSVLYYDSSNNVILRKHRNMVVKACGCH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181809
• ORF Size: 1206 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181809.4
• RefSeq Size: 1692 bp
• RefSeq ORF: 1209 bp
• Locus ID: 353500
• UniProt ID: Q7Z5Y6
• Cytogenetics: 1p34.3
• Protein Families: Secreted Protein
• Protein Pathways: Hedgehog signaling pathway, TGF-beta signaling pathway
• MW: 44.6 kDa
• Gene Summary: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein may play a role in development of the reproductive system. This gene may have arose from a gene duplication event and its gene duplicate is also present on chromosome 1. [provided by RefSeq, Jul 2016]