LRSAM1 (NM_138361) Human Tagged ORF Clone

CAT#: RC215025

LRSAM1 (Myc-DDK-tagged)-Human leucine rich repeat and sterile alpha motif containing 1 (LRSAM1), transcript variant 1

Product Images

Circular map for RC215025

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  Western blot validation of overexpression lysate (Cat# LY423707) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223778 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified LRSAM1 protein (Cat# TP315025). The protein was produced from HEK293T cells transfected with LRSAM1 cDNA clone (Cat# RC215025) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LRSAM1
• Synonyms: CMT2P; RIFLE; TAL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215025 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCTCTTCTTCCGGAAGCGGAAACCCAGTGAGGAGGCTCGGAAACGCCTGGAGTACCAGATGTGTT
  TGGCAAAAGAAGCTGGGGCAGATGACATTCTCGACATCTCTAAATGTGAGCTCTCAGAGATTCCATTTGG
  AGCTTTTGCAACATGCAAAGTTCTGCAGAAGAAGGTGCTGATCGTCCACACGAATCACCTCACTTCCCTG
  CTTCCCAAATCCTGCAGCCTCCTGAGTCTGGCAACCATTAAGGTTCTAGATCTCCACGATAATCAGCTGA
  CAGCCCTTCCTGACGATCTGGGGCAGCTGACTGCCCTCCAGGTCTTAAACGTGGAAAGGAATCAACTGAT
  GCAGCTCCCACGTTCCATTGGGAACCTGACCCAGCTCCAGACTCTCAATGTTAAAGACAACAAGCTGAAG
  GAGCTTCCAGACACCGTGGGGGAGCTTCGAAGCCTGCGTACCCTCAACATCAGTGGAAACGAGATCCAGA
  GATTGCCGCAGATGCTGGCTCACGTTCGAACCCTGGAGATGCTGAGCCTTGACGCCTCGGCCATGGTCTA
  CCCGCCGCGGGAGGTGTGTGGTGCCGGCACTGCGGCCATCTTGCAGTTCCTCTGCAAAGAGTCAGGGCTG
  GAATACTACCCCCCTTCTCAGTACTTGCTGCCAATTCTGGAGCAAGATGGAATCGAGAACTCTCGGGACA
  GCCCTGATGGGCCCACGGACAGATTCTCAAGGGAGGAGTTAGAGTGGCAGAACAGGTTCTCAGACTATGA
  GAAGAGGAAGGAACAGAAGATGCTGGAGAAACTCGAGTTTGAACGGCGCCTGGAACTGGGGCAGCGGGAG
  CACACCCAGCTCCTTCAGCAGAGCAGCAGCCAGAAGGATGAGATCCTTCAGACGGTCAAGGAGGAGCAGT
  CCCGGCTGGAGCAGGGCCTGAGTGAGCACCAGCGCCACCTCGACGCAGAGCGGCAGCGGCTGCAGGAGCA
  GCTGAAGCAGACGGAACAGAACATTTCCAGCCGGATCCAGAAGCTGCTGCAGGACAATCAGAGACAAAAG
  AAAAGCTCCGAGATTTTGAAATCGCTGGAAAATGAAAGAATAAGAATGGAACAGTTGATGTCCATAACCC
  AGGAGGAGACTGAGAGCCTGCGGCGACGTGACGTTGCCTCCGCCATGCAGCAGATGCTGACTGAGAGCTG
  TAAGAACCGGCTCATCCAGATGGCCTACGAATCTCAGAGGCAGAACTTGGTCCAGCAGGCCTGTTCCAGC
  ATGGCCGAAATGGATGAACGATTCCAGCAGATTCTGTCGTGGCAGCAAATGGATCAGAACAAAGCCATCA
  GCCAGATCCTGCAGGAGAGCGCGATGCAGAAGGCTGCGTTCGAGGCACTCCAGGTGAAGAAAGACCTGAT
  GCATCGGCAGATCAGGAGCCAGATTAAGTTAATAGAAACTGAGTTATTGCAGCTGACACAGCTGGAGTTA
  AAGAGGAAGTCCCTGGACACAGAGTCACTCCAGGAGATGATCTCGGAGCAGCGCTGGGCCCTCAGCTCCC
  TGCTCCAGCAGCTGCTCAAAGAGAAGCAGCAGCGAGAGGAAGAGCTCCGGGAAATCCTGACGGAGTTAGA
  AGCCAAAAGTGAAACCAGGCAGGAAAATTACTGGCTGATTCAGTATCAACGGCTTTTGAACCAGAAGCCC
  TTGTCCTTGAAGCTGCAAGAAGAGGGGATGGAGCGCCAGCTGGTGGCCCTCCTGGAGGAGCTGTCGGCTG
  AGCACTACCTGCCCATCTTTGCGCACCACCGCCTCTCACTGGACCTGCTGAGCCAAATGAGCCCAGGGGA
  CCTGGCCAAGGTGGGCGTCTCAGAAGCTGGCCTGCAGCACGAGATCCTCCGGAGAGTCCAGGAACTGCTG
  GATGCAGCCAGGATCCAGCCAGAGCTGAAACCACCAATGGGTGAGGTCGTCACCCCTACGGCCCCCCAGG
  AGCCTCCTGAGTCTGTGAGGCCATCCGCTCCCCCTGCAGAGCTGGAGGTGCAGGCCTCAGAGTGTGTCGT
  GTGCCTGGAACGGGAGGCCCAGATGATCTTCCTCAACTGTGGCCACGTCTGCTGCTGCCAGCAGTGCTGC
  CAGCCACTGCGCACCTGCCCGCTGTGCCGCCAGGACATCGCCCAGCGCCTCCGCATCTACCACAGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215025 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPLFFRKRKPSEEARKRLEYQMCLAKEAGADDILDISKCELSEIPFGAFATCKVLQKKVLIVHTNHLTSL
  LPKSCSLLSLATIKVLDLHDNQLTALPDDLGQLTALQVLNVERNQLMQLPRSIGNLTQLQTLNVKDNKLK
  ELPDTVGELRSLRTLNISGNEIQRLPQMLAHVRTLEMLSLDASAMVYPPREVCGAGTAAILQFLCKESGL
  EYYPPSQYLLPILEQDGIENSRDSPDGPTDRFSREELEWQNRFSDYEKRKEQKMLEKLEFERRLELGQRE
  HTQLLQQSSSQKDEILQTVKEEQSRLEQGLSEHQRHLDAERQRLQEQLKQTEQNISSRIQKLLQDNQRQK
  KSSEILKSLENERIRMEQLMSITQEETESLRRRDVASAMQQMLTESCKNRLIQMAYESQRQNLVQQACSS
  MAEMDERFQQILSWQQMDQNKAISQILQESAMQKAAFEALQVKKDLMHRQIRSQIKLIETELLQLTQLEL
  KRKSLDTESLQEMISEQRWALSSLLQQLLKEKQQREEELREILTELEAKSETRQENYWLIQYQRLLNQKP
  LSLKLQEEGMERQLVALLEELSAEHYLPIFAHHRLSLDLLSQMSPGDLAKVGVSEAGLQHEILRRVQELL
  DAARIQPELKPPMGEVVTPTAPQEPPESVRPSAPPAELEVQASECVVCLEREAQMIFLNCGHVCCCQQCC
  QPLRTCPLCRQDIAQRLRIYHSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138361
• ORF Size: 2169 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138361.1
• RefSeq Size: 3405 bp
• RefSeq ORF: 2172 bp
• Locus ID: 90678
• UniProt ID: Q6UWE0
• Cytogenetics: 9q33.3-q34.11
• Domains: LRR, SAM, LRR_TYP, LRR_BAC, LRR_PS
• Protein Families: Druggable Genome
• MW: 83.6 kDa
• Gene Summary: This gene encodes a ring finger protein involved in a variety of functions, including regulation of signaling pathways and cell adhesion, mediation of self-ubiquitylation, and involvement in cargo sorting during receptor endocytosis. Mutations in this gene have been associated with Charcot-Marie-Tooth disease. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jan 2012]