hSET1 (SETD1A) (NM_014712) Human Tagged ORF Clone

CAT#: RC214996

SETD1A (Myc-DDK-tagged)-Human SET domain containing 1A (SETD1A)

Product Images

Circular map for RC214996

• 

  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY SETD1A (Cat# RC214996, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SETD1A(Cat# TA805877). Positive lysates LY415047 (100ug) and LC415047 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY415047) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214996 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: hSET1
• Synonyms: EPEDD; KMT2F; NEDSID; Set1; Set1A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214996 representing NM_014712
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATCAGGAAGGTGGGGGAGATGGGCAGAAGGCCCCGAGCTTCCAGTGGCGGAACTACAAGCTCATCG
  TGGATCCTGCCTTGGACCCTGCCCTGCGCAGGCCTTCTCAGAAGGTGTACCGCTATGATGGAGTCCACTT
  CAGTGTCAACGACTCAAAGTATATACCAGTCGAAGACCTCCAAGACCCCCGTTGCCATGTCAGGTCCAAA
  AACAGAGACTTTTCCCTCCCAGTCCCTAAGTTTAAGCTGGACGAGTTCTATATTGGACAGATTCCACTGA
  AGGAAGTGACTTTTGCAAGGCTGAATGACAACGTGCGGGAGACCTTCCTGAAGGATATGTGCCGTAAGTA
  CGGTGAGGTGGAAGAGGTAGAGATCCTCCTTCACCCCCGTACGCGCAAGCACCTGGGCCTGGCCCGTGTG
  CTCTTCACCAGCACTCGGGGCGCCAAGGAAACGGTCAAAAACCTCCACCTTACCTCCGTCATGGGCAACA
  TCATCCATGCCCAGCTTGACATCAAAGGACAACAACGAATGAAATACTATGAACTAATTGTCAATGGCTC
  CTACACCCCTCAGACTGTGCCCACTGGGGGCAAGGCCCTGAGTGAGAAGTTCCAAGGCTCGGGTGCAGCC
  ACTGAGACGGCCGAATCCCGCCGCCGCTCTTCCTCTGACACAGCTGCCTACCCAGCAGGCACCACTGCGG
  TGGGCACTCCTGGCAACGGCACCCCCTGCTCCCAGGACACAAGCTTCTCCAGCAGCCGACAAGATACCCC
  ATCTTCCTTTGGCCAGTTCACACCTCAGTCCTCCCAAGGAACCCCCTACACGTCTCGGGGCAGCACCCCC
  TACTCTCAGGACTCTGCCTACTCCAGCAGCACCACTTCAACCTCCTTCAAGCCCCGGCGGTCAGAGAACA
  GCTACCAAGATGCCTTTTCCCGCCGCCACTTCTCTGCATCTTCAGCCTCCACAACCGCCTCCACGGCCAT
  CGCCGCCACCACTGCAGCCACTGCCTCATCCTCCGCCTCTTCCTCCTCATTGTCCTCGTCCTCCTCGTCA
  TCCTCTTCCTCCTCGTCCTCTCAGTTTCGTAGTTCTGATGCAAACTACCCAGCGTATTATGAAAGCTGGA
  ATCGCTACCAGCGCCATACTTCCTACCCACCACGCCGGGCCACACGGGAGGAACCCCCTGGAGCCCCTTT
  TGCTGAAAATACAGCTGAGCGCTTCCCACCTTCTTACACCTCCTACCTGCCCCCCGAGCCCAGCCGGCCC
  ACCGACCAGGACTACCGGCCTCCTGCCTCAGAGGCTCCACCCCCGGAGCCTCCAGAACCTGGTGGAGGCG
  GGGGTGGAGGAGGGCCCAGCCCTGAGAGAGAAGAAGTTCGGACTTCCCCCCGCCCAGCCTCCCCTGCCCG
  CTCTGGCTCCCCAGCCCCGGAGACCACCAATGAGAGTGTGCCCTTCGCCCAGCACAGCAGCCTGGATTCC
  CGCATCGAGATGCTGCTGAAGGAGCAGCGCTCCAAGTTTTCCTTCTTGGCCTCTGACACAGAGGAGGAGG
  AAGAGAACAGCAGCATGGTCCTTGGGGCCAGAGATACAGGGAGTGAGGTGCCTTCTGGGTCAGGGCATGG
  GCCCTGCACACCCCCTCCGGCCCCAGCTAATTTTGAGGATGTGGCACCTACAGGGAGCGGGGAGCCAGGG
  GCTACCCGGGAGTCTCCCAAGGCAAATGGACAGAACCAGGCTTCTCCATGCTCTTCTGGAGACGACATGG
  AGATCTCCGACGACGACCGGGGTGGCTCACCCCCTCCGGCCCCGACGCCCCCTCAGCAGCCTCCGCCACC
  TCCCCCTCCCCCGCCGCCTCCTCCTCCCTACCTGGCGTCCCTTCCTCTTGGTTATCCTCCCCACCAACCT
  GCCTACCTCCTCCCACCCAGACCTGATGGGCCGCCGCCCCCTGAGTACCCCCCACCTCCTCCACCACCCC
  CGCACATCTATGACTTTGTGAACTCCTTGGAGCTCATGGACCGACTTGGGGCTCAGTGGGGAGGGATGCC
  CATGTCCTTCCAGATGCAGACCCAGATGTTAACTCGGCTCCATCAGCTGCGGCAGGGCAAGGGATTGATT
  GCCGCCTCAGCTGGCCCCCCCGGTGGGGCCTTTGGGGAGGCCTTCCTCCCGTTTCCACCCCCGCAGGAGG
  CAGCCTACGGCTTGCCGTATGCTCTATATGCACAGGGGCAGGAGGGCAGAGGGGCATACTCACGGGAGGC
  CTACCACCTGCCCATGCCAATGGCAGCCGAGCCCCTGCCCTCCTCCTCAGTCTCGGGAGAGGAGGCCCGG
  CTGCCACCCAGGGAAGAAGCAGAGCTGGCAGAGGGCAAGACCCTCCCGACAGCAGGCACCGTGGGCCGTG
  TGCTCGCCATGCTGGTCCAGGAGATGAAGAGCATCATGCAGCGAGACCTCAACCGCAAGATGGTGGAGAA
  CGTGGCCTTCGGAGCCTTTGACCAGTGGTGGGAGAGCAAGGAGGAGAAGGCCAAGCCATTCCAGAACGCG
  GCCAAGCAGCAAGCCAAGGAGGAGGATAAAGAGAAGACGAAGCTGAAGGAGCCTGGCCTGCTGTCCCTCG
  TGGACTGGGCCAAGAGCGGGGGCACTACGGGCATCGAGGCTTTCGCCTTTGGGTCAGGGCTGAGAGGGGC
  CCTGCGGCTGCCTTCATTCAAGGTAAAGCGGAAAGAGCCATCGGAAATTTCCGAGGCCAGTGAGGAAAAG
  AGGCCTCGTCCCTCCACTCCTGCTGAGGAAGATGAAGACGACCCTGAACAAGAGAAGGAGGCTGGAGAGC
  CAGGACGTCCGGGGACCAAGCCCCCGAAGCGGGACGAAGAGCGAGGCAAGACCCAGGGCAAGCACCGCAA
  GTCCTTTGCTCTGGACAGCGAAGGGGAGGAGGCATCCCAGGAGTCCTCCTCGGAGAAGGATGAGGAGGAT
  GACGAGGAAGATGAGGAAGATGAAGATCGAGAGGAAGCTGTGGATACCACAAAGAAGGAGACAGAGGTGT
  CGGATGGCGAGGACGAGGAAAGCGATTCGTCTTCCAAATGTTCTCTGTATGCTGACTCAGATGGCGAAAA
  TGACAGCACATCAGACTCCGAGAGCAGCAGCTCTTCCAGCTCCTCATCCTCCTCCTCCTCCTCGTCCTCA
  TCCTCCTCGTCCTCTTCATCCTCTGAGTCCTCCTCTGAAGATGAAGAGGAAGAGGAGCGGCCAGCAGCCC
  TTCCCTCAGCCTCCCCGCCCCCCAGAGAAGTCCCAGTGCCCACGCCAGCACCTGTGGAGGTGCCAGTGCC
  GGAAAGGGTTGCAGGCTCCCCAGTCACACCCCTGCCCGAACAGGAGGCGTCTCCAGCAAGGCCTGCAGGC
  CCCACGGAGGAGTCACCCCCCAGTGCGCCTCTGCGTCCCCCAGAACCACCTGCTGGGCCCCCGGCCCCTG
  CCCCACGCCCCGATGAGCGTCCCTCTTCTCCCATCCCCCTCCTGCCCCCACCCAAGAAACGCCGGAAAAC
  TGTCTCCTTCTCTGCCATCGAGGTGGTGCCAGCCCCGGAGCCCCCTCCAGCCACACCGCCGCAGGCCAAG
  TTTCCCGGCCCAGCCTCCCGCAAGGCTCCCCGGGGCGTGGAGCGGACCATCCGCAACCTGCCCCTGGACC
  ACGCATCTCTGGTCAAGAGTTGGCCCGAGGAGGTGTCCCGAGGAGGCCGGAGCCGGGCTGGAGGCCGAGG
  CCGCCTCACCGAGGAAGAGGAGGCTGAGCCAGGGACAGAGGTGGACCTGGCGGTCCTGGCCGACCTGGCC
  CTGACCCCTGCCCGGCGCGGGCTGCCTGCCCTGCCTGCTGTTGAAGACTCAGAGGCCACAGAGACATCGG
  ACGAGGCCGAGCGCCCTAGGCCCCTGCTCAGCCACATCCTCCTGGAGCACAACTATGCCCTGGCCGTCAA
  GCCCACGCCCCCTGCGCCAGCCCTGCGGCCCCCGGAGCCAGTGCCCGCACCCGCCGCCCTCTTCAGTTCC
  CCAGCTGATGAGGTCCTGGAGGCCCCCGAGGTGGTGGTGGCTGAGGCGGAGGAGCCCAAGCCGCAGCAAC
  TGCAGCAGCAGCGGGAGGAGGGCGAAGAGGAGGGGGAGGAAGAGGGGGAGGAAGAGGAGGAGGAGTCCTC
  TGACAGCAGCAGCAGCAGCGATGGGGAGGGCGCCCTCCGGAGGCGCAGCCTCCGCTCCCACGCCCGGCGC
  CGCCGCCCTCCGCCCCCACCCCCGCCGCCACCGCCCCGCGCCTACGAGCCACGCAGTGAGTTTGAACAGA
  TGACCATCCTGTATGACATTTGGAACTCGGGCCTGGACTCAGAGGACATGAGTTACCTGCGGCTTACGTA
  CGAGCGGCTGCTGCAGCAGACAAGCGGGGCTGACTGGCTCAACGACACTCACTGGGTCCATCACACAATC
  ACCAACCTGACCACCCCAAAACGCAAGCGGCGGCCCCAGGATGGGCCCCGGGAGCACCAGACAGGCTCAG
  CCCGCAGCGAAGGCTACTACCCCATCAGCAAGAAGGAGAAGGACAAGTACCTGGACGTGTGCCCAGTCTC
  GGCCCGGCAGCTGGAGGGCGTGGACACTCAGGGGACGAACCGCGTGCTGTCCGAGCGCCGGTCCGAGCAG
  CGGCGGCTGCTGAGCGCCATCGGTACCTCCGCCATCATGGACAGTGACCTGCTGAAACTCAACCAGCTCA
  AGTTCCGGAAGAAGAAGCTCCGATTTGGCCGGAGCCGGATCCACGAGTGGGGTCTGTTTGCCATGGAACC
  CATTGCTGCTGACGAGATGGTCATCGAATACGTGGGTCAGAACATCCGTCAGATGGTGGCCGACATGCGG
  GAGAAGCGCTACGTGCAGGAGGGCATTGGCAGCAGCTACCTGTTCCGGGTGGACCACGACACCATCATCG
  ATGCCACCAAGTGTGGCAACCTGGCCAGATTCATCAACCACTGCTGCACGCCTAACTGCTACGCCAAGGT
  CATCACCATCGAGTCCCAGAAGAAGATCGTGATCTACTCCAAGCAGCCCATTGGCGTGGACGAGGAGATC
  ACCTACGACTACAAGTTCCCACTGGAAGACAACAAGATCCCGTGTCTGTGTGGCACAGAGAGCTGCCGGG
  GCTCCCTAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214996 representing NM_014712
  Red=Cloning site Green=Tags(s)
  
  MDQEGGGDGQKAPSFQWRNYKLIVDPALDPALRRPSQKVYRYDGVHFSVNDSKYIPVEDLQDPRCHVRSK
  NRDFSLPVPKFKLDEFYIGQIPLKEVTFARLNDNVRETFLKDMCRKYGEVEEVEILLHPRTRKHLGLARV
  LFTSTRGAKETVKNLHLTSVMGNIIHAQLDIKGQQRMKYYELIVNGSYTPQTVPTGGKALSEKFQGSGAA
  TETAESRRRSSSDTAAYPAGTTAVGTPGNGTPCSQDTSFSSSRQDTPSSFGQFTPQSSQGTPYTSRGSTP
  YSQDSAYSSSTTSTSFKPRRSENSYQDAFSRRHFSASSASTTASTAIAATTAATASSSASSSSLSSSSSS
  SSSSSSSQFRSSDANYPAYYESWNRYQRHTSYPPRRATREEPPGAPFAENTAERFPPSYTSYLPPEPSRP
  TDQDYRPPASEAPPPEPPEPGGGGGGGGPSPEREEVRTSPRPASPARSGSPAPETTNESVPFAQHSSLDS
  RIEMLLKEQRSKFSFLASDTEEEEENSSMVLGARDTGSEVPSGSGHGPCTPPPAPANFEDVAPTGSGEPG
  ATRESPKANGQNQASPCSSGDDMEISDDDRGGSPPPAPTPPQQPPPPPPPPPPPPPYLASLPLGYPPHQP
  AYLLPPRPDGPPPPEYPPPPPPPPHIYDFVNSLELMDRLGAQWGGMPMSFQMQTQMLTRLHQLRQGKGLI
  AASAGPPGGAFGEAFLPFPPPQEAAYGLPYALYAQGQEGRGAYSREAYHLPMPMAAEPLPSSSVSGEEAR
  LPPREEAELAEGKTLPTAGTVGRVLAMLVQEMKSIMQRDLNRKMVENVAFGAFDQWWESKEEKAKPFQNA
  AKQQAKEEDKEKTKLKEPGLLSLVDWAKSGGTTGIEAFAFGSGLRGALRLPSFKVKRKEPSEISEASEEK
  RPRPSTPAEEDEDDPEQEKEAGEPGRPGTKPPKRDEERGKTQGKHRKSFALDSEGEEASQESSSEKDEED
  DEEDEEDEDREEAVDTTKKETEVSDGEDEESDSSSKCSLYADSDGENDSTSDSESSSSSSSSSSSSSSSS
  SSSSSSSSESSSEDEEEEERPAALPSASPPPREVPVPTPAPVEVPVPERVAGSPVTPLPEQEASPARPAG
  PTEESPPSAPLRPPEPPAGPPAPAPRPDERPSSPIPLLPPPKKRRKTVSFSAIEVVPAPEPPPATPPQAK
  FPGPASRKAPRGVERTIRNLPLDHASLVKSWPEEVSRGGRSRAGGRGRLTEEEEAEPGTEVDLAVLADLA
  LTPARRGLPALPAVEDSEATETSDEAERPRPLLSHILLEHNYALAVKPTPPAPALRPPEPVPAPAALFSS
  PADEVLEAPEVVVAEAEEPKPQQLQQQREEGEEEGEEEGEEEEEESSDSSSSSDGEGALRRRSLRSHARR
  RRPPPPPPPPPPRAYEPRSEFEQMTILYDIWNSGLDSEDMSYLRLTYERLLQQTSGADWLNDTHWVHHTI
  TNLTTPKRKRRPQDGPREHQTGSARSEGYYPISKKEKDKYLDVCPVSARQLEGVDTQGTNRVLSERRSEQ
  RRLLSAIGTSAIMDSDLLKLNQLKFRKKKLRFGRSRIHEWGLFAMEPIAADEMVIEYVGQNIRQMVADMR
  EKRYVQEGIGSSYLFRVDHDTIIDATKCGNLARFINHCCTPNCYAKVITIESQKKIVIYSKQPIGVDEEI
  TYDYKFPLEDNKIPCLCGTESCRGSLN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014712
• ORF Size: 5121 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014712.3
• RefSeq Size: 6447 bp
• RefSeq ORF: 5124 bp
• Locus ID: 9739
• UniProt ID: O15047
• Cytogenetics: 16p11.2
• Protein Families: Druggable Genome
• Protein Pathways: Lysine degradation
• MW: 185.9 kDa
• Gene Summary: The protein encoded by this gene is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. Trimethylation of histone H3 at lysine 4 (H3K4me3) is a chromatin modification known to generally mark the transcription start sites of active genes. The protein contains SET domains, a RNA recognition motif domain and is a member of the class V-like SAM-binding methyltransferase superfamily. [provided by RefSeq, Dec 2016]