ZNF264 (NM_003417) Human Tagged ORF Clone

CAT#: RC214965

ZNF264 (Myc-DDK-tagged)-Human zinc finger protein 264 (ZNF264)

Product Images

Circular map for RC214965

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ZNF264 (Cat# RC214965, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZNF264 (Cat# TA810495)(1:2000). Positive lysates LY418709 (100ug) and LC418709 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY418709) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214965 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF264
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214965 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCAGCGGTGCTGACGGACCGGGCCCAGGTGTCTGTGACCTTTGATGATGTGGCTGTGACTTTCA
  CCAAGGAGGAGTGGGGGCAGCTGGACCTAGCTCAGCGGACCCTGTACCAGGAGGTGATGCTGGAAAACTG
  TGGGCTCCTGGTGTCTCTGGGGTGTCCTGTTCCCAAAGCTGAGCTGATCTGCCACCTAGAGCATGGGCAG
  GAGCCATGGACCAGGAAGGAAGACCTCTCCCAAGACACCTGTCCAGGCGACAAAGGAAAACCTAAGACCA
  CAGAACCTACCACTTGTGAGCCAGCCTTGTCAGAGGGAATCTCACTTCAGGGACAAGTGACACAAGGAAA
  CTCAGTGGACTCACAGTTGGGGCAAGCCGAGGATCAGGATGGGCTATCAGAAATGCAGGAAGGACACTTC
  AGACCAGGAATAGATCCCCAGGAGAAGTCTCCTGGGAAGATGAGCCCTGAATGTGATGGTTTAGGGACAG
  CTGATGGTGTGTGTTCAAGGATTGGACAGGAGCAAGTCTCTCCAGGAGATACAGTCCATAGCCATAACTC
  ATGTGAGTCAGGTAAAGATCCCATGATTCAGGAAGAGGAAAATAACTTTAAATGCAGTGAATGTGGAAAA
  GTATTTAACAAGAAACACCTCCTTGCTGGACATGAGAAAATTCACTCTGGAGTTAAGCCCTATGAATGCA
  CAGAATGTGGGAAAACCTTTATTAAGAGCACACATCTCCTGCAACATCACATGATCCACACTGGGGAGAG
  GCCCTATGAGTGCATGGAGTGTGGAAAGGCCTTCAACCGCAAGTCATACCTTACCCAGCACCAGCGGATT
  CACAGTGGAGAGAAGCCTTACAAGTGCAATGAATGCGGAAAGGCCTTCACCCACCGCTCCAATTTTGTCT
  TGCATAACAGGAGACACACTGGAGAAAAATCCTTTGTGTGCACAGAATGTGGCCAAGTCTTTCGACATAG
  GCCAGGCTTTCTCCGGCACTATGTTGTCCACAGTGGTGAGAATCCCTATGAGTGCTTGGAGTGTGGCAAG
  GTCTTCAAACACAGGTCATATCTCATGTGGCACCAGCAGACTCATACCGGGGAGAAGCCCTATGAGTGCA
  GTGAATGTGGGAAGGTCTTCTTGGAGAGTGCAGCCCTGATTCACCACTATGTCATCCACACTGGAGAGAA
  GCCCTTTGAGTGCCTCGAGTGTGGGAAGGCTTTCAACCACCGATCCTACCTCAAGAGGCACCAGCGGATT
  CACACTGGGGAGAAGCCCTTCGTGTGCAGTGAATGTGGAAAGGCCTTCACCCACTGCTCTACTTTTATCT
  TGCATAAAAGGGCCCACACTGGAGAAAAGCCTTTCGAGTGCAAAGAGTGTGGGAAAGCCTTTAGCAATCG
  GAAGGACCTCATTCGCCACTTCAGCATCCACACTGGAGAGAAGCCCTATGAGTGCGTGGAGTGTGGAAAG
  GCCTTCACCCGCATGTCGGGCCTCACGAGGCACAAGCGGATTCATAGTGGAGAGAAGCCCTATGAATGTG
  TTGAGTGTGGGAAATCGTTTTGCTGGAGCACAAACCTCATTCGACATGCCATTATCCACACTGGAGAGAA
  GCCCTATAAATGTAGTGAATGTGGAAAGGCCTTCAGTCGCAGCTCGTCCCTCACTCAGCATCAAAGGATG
  CATACTGGGAAAAATCCCATCAGTGTAACAGATGTGGGAAGACCTTTTACAAGTGGACAAACCTCAGTTA
  CCCTTCGAGAACTTCTTTTAGGGAAGGACTTTTTGAATGTAACCACTGAGGCAAATATTTTGCCAGAGGA
  AACATCTTCCTCTGCATCTGATCAACCATACCAAAGAGAAACCCCACAAGTGTCTTCACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214965 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAAVLTDRAQVSVTFDDVAVTFTKEEWGQLDLAQRTLYQEVMLENCGLLVSLGCPVPKAELICHLEHGQ
  EPWTRKEDLSQDTCPGDKGKPKTTEPTTCEPALSEGISLQGQVTQGNSVDSQLGQAEDQDGLSEMQEGHF
  RPGIDPQEKSPGKMSPECDGLGTADGVCSRIGQEQVSPGDTVHSHNSCESGKDPMIQEEENNFKCSECGK
  VFNKKHLLAGHEKIHSGVKPYECTECGKTFIKSTHLLQHHMIHTGERPYECMECGKAFNRKSYLTQHQRI
  HSGEKPYKCNECGKAFTHRSNFVLHNRRHTGEKSFVCTECGQVFRHRPGFLRHYVVHSGENPYECLECGK
  VFKHRSYLMWHQQTHTGEKPYECSECGKVFLESAALIHHYVIHTGEKPFECLECGKAFNHRSYLKRHQRI
  HTGEKPFVCSECGKAFTHCSTFILHKRAHTGEKPFECKECGKAFSNRKDLIRHFSIHTGEKPYECVECGK
  AFTRMSGLTRHKRIHSGEKPYECVECGKSFCWSTNLIRHAIIHTGEKPYKCSECGKAFSRSSSLTQHQRM
  HTGKNPISVTDVGRPFTSGQTSVTLRELLLGKDFLNVTTEANILPEETSSSASDQPYQRETPQVSSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003417
• ORF Size: 1881 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003417.5
• RefSeq Size: 12172 bp
• RefSeq ORF: 1884 bp
• Locus ID: 9422
• UniProt ID: O43296
• Cytogenetics: 19q13.43
• Domains: KRAB, zf-C2H2
• Protein Families: Transcription Factors
• MW: 70.5 kDa
• Gene Summary: This gene encodes a zinc finger protein and belongs to the krueppel C2H2-type zinc-finger protein family. Zinc finger proteins are often localized in the nucleus, bind nucleic acids, and regulate transcription. [provided by RefSeq, Jan 2010]