UBTD2 (NM_152277) Human Tagged ORF Clone

CAT#: RC214938

UBTD2 (Myc-DDK-tagged)-Human ubiquitin domain containing 2 (UBTD2)

Product Images

Circular map for RC214938

• 

  Western blot validation of overexpression lysate (Cat# LY407697) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214938 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UBTD2
• Synonyms: DCUBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214938 representing NM_152277
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGGGTGTGTGGGCGCCCAGCACGACTCCTCGGGCAGCCTCAACGAGAACTCGGAGGGCACCGGAG
  TTGCTCTAGGTCGTAACCAGCCTTTGAAAAAGGAGAAACCAAAATGGAAAAGCGATTATCCTATGACAGA
  TGGACAACTACGCAGCAAGAGGGATGAATTTTGGGATACAGCACCAGCTTTTGAAGGCCGGAAAGAGATT
  TGGGATGCCTTGAAGGCTGCTGCACATGCTTTTGAGAGCAATGATCATGAACTGGCACAAGCAATCATTG
  ATGGTGCAAACATAACATTACCACATGGTGCACTTACAGAGTGCTACGATGAACTGGGGAACAGATATCA
  GCTTCCAGTGTATTGCTTGGCACCGCCAATCAACATGATAGAGGAAAAGAGCGACATAGAGACTCTGGAT
  ATTCCTGAGCCACCACCCAATTCTGGATATGAATGTCAGCTTCGTTTGCGCCTTTCCACAGGCAAAGACC
  TCAAGCTTGTGGTTCGCAGCACAGACACAGTATTCCACATGAAGAGACGGTTGCATGCAGCAGAGGGAGT
  GGAACCAGGTAGTCAGCGGTGGTTTTTTTCTGGCAGACCTCTCACTGACAAAATGAAGTTCGAAGAGCTG
  AAGATCCCAAAGGACTATGTTGTACAGGTTATAGTGAGCCAACCTGTGCAGAACCCAACACCAGTGGAGA
  AC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214938 representing NM_152277
  Red=Cloning site Green=Tags(s)
  
  MGGCVGAQHDSSGSLNENSEGTGVALGRNQPLKKEKPKWKSDYPMTDGQLRSKRDEFWDTAPAFEGRKEI
  WDALKAAAHAFESNDHELAQAIIDGANITLPHGALTECYDELGNRYQLPVYCLAPPINMIEEKSDIETLD
  IPEPPPNSGYECQLRLRLSTGKDLKLVVRSTDTVFHMKRRLHAAEGVEPGSQRWFFSGRPLTDKMKFEEL
  KIPKDYVVQVIVSQPVQNPTPVEN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152277
• ORF Size: 702 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152277.3
• RefSeq Size: 3015 bp
• RefSeq ORF: 705 bp
• Locus ID: 92181
• UniProt ID: Q8WUN7
• Cytogenetics: 5q35.1
• Domains: UBQ
• Protein Families: Druggable Genome
• MW: 26 kDa