TDP1 (NM_018319) Human Tagged ORF Clone

CAT#: RC214927

TDP1 (Myc-DDK-tagged)-Human tyrosyl-DNA phosphodiesterase 1 (TDP1), transcript variant 1

Product Images

Circular map for RC214927

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TDP1 (Cat# RC214927, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TDP1(Cat# TA503768). Positive lysates LY413162 (100ug) and LC413162 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY413162) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214927 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TDP1 protein (Cat# TP314927). The protein was produced from HEK293T cells transfected with TDP1 cDNA clone (Cat# RC214927) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TDP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214927 representing NM_018319
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCAGGAAGGCGATTATGGGAGGTGGACCATATCTAGTAGTGATGAAAGTGAGGAAGAAAAGCCAA
  AACCAGACAAGCCATCTACCTCTTCTCTTCTCTGTGCCAGGCAAGGAGCAGCAAATGAGCCCAGGTACAC
  CTGTTCCGAGGCCCAGAAAGCTGCACACAAGAGGAAAATATCACCTGTGAAATTCAGCAATACAGATTCA
  GTTTTACCTCCCAAAAGGCAGAAAAGCGGTTCCCAGGAGGACCTCGGCTGGTGTCTGTCCAGCAGTGATG
  ATGAGCTGCAACCAGAAATGCCGCAGAAGCAGGCTGAGAAAGTGGTGATCAAAAAGGAGAAAGACATCTC
  TGCTCCCAATGACGGCACTGCCCAAAGAACTGAAAATCATGGCGCTCCCGCCTGCCACAGGCTCAAAGAG
  GAGGAAGACGAGTATGAGACATCAGGGGAGGGCCAGGACATTTGGGACATGCTGGATAAAGGGAACCCCT
  TCCAGTTTTACCTCACTAGAGTCTCTGGAGTTAAGCCAAAGTATAACTCTGGAGCCCTCCACATCAAGGA
  TATTTTATCTCCTTTATTTGGGACGCTTGTTTCTTCAGCTCAGTTTAACTACTGCTTTGACGTGGACTGG
  CTCGTAAAACAGTATCCACCAGAGTTCAGGAAGAAGCCAATCCTGCTTGTGCATGGTGATAAGCGAGAGG
  CTAAGGCTCACCTCCATGCCCAGGCCAAGCCTTACGAGAACATCTCTCTCTGCCAGGCAAAGTTGGATAT
  TGCGTTTGGAACACACCACACGAAAATGATGCTGCTGCTCTATGAAGAAGGCCTCCGGGTTGTCATACAC
  ACCTCCAACCTCATCCATGCTGACTGGCACCAGAAAACTCAAGGAATATGGTTGAGCCCCTTATACCCAC
  GAATTGCTGATGGAACCCACAAATCTGGAGAGTCGCCAACACATTTTAAAGCTGATCTCATCAGTTACTT
  GATGGCTTATAATGCCCCTTCTCTCAAGGAGTGGATAGATGTCATTCACAAGCACGATCTCTCTGAAACA
  AATGTTTATCTTATTGGTTCAACCCCAGGACGCTTTCAAGGAAGTCAAAAAGATAATTGGGGACATTTTA
  GACTTAAGAAGCTTCTGAAAGACCATGCCTCATCCATGCCTAACGCAGAGTCCTGGCCTGTCGTAGGTCA
  GTTTTCAAGCGTTGGCTCCTTGGGAGCCGATGAATCAAAGTGGTTATGTTCTGAGTTTAAAGAGAGCATG
  CTGACACTGGGGAAGGAAAGCAAGACTCCAGGAAAAAGCTCTGTTCCTCTTTACTTGATCTATCCTTCTG
  TGGAAAATGTGCGGACCAGTTTAGAAGGATATCCTGCTGGGGGCTCTCTTCCCTATAGCATCCAGACAGC
  TGAAAAACAGAATTGGCTGCATTCCTATTTTCACAAATGGTCAGCTGAGACTTCTGGCCGCAGCAATGCC
  ATGCCACATATTAAGACATATATGAGGCCTTCTCCAGACTTCAGTAAAATTGCTTGGTTCCTTGTCACAA
  GCGCAAATCTGTCCAAGGCTGCCTGGGGAGCATTGGAGAAGAATGGCACCCAGCTGATGATCCGCTCCTA
  CGAGCTCGGGGTCCTTTTCCTCCCTTCAGCATTTGGTCTAGACAGTTTCAAAGTGAAACAGAAGTTCTTC
  GCTGGCAGCCAGGAGCCAATGGCCACCTTTCCTGTGCCATATGATTTGCCTCCAGAACTGTATGGAAGTA
  AAGATCGGCCATGGATATGGAACATTCCTTATGTCAAAGCACCGGATACGCATGGGAACATGTGGGTGCC
  CTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214927 representing NM_018319
  Red=Cloning site Green=Tags(s)
  
  MSQEGDYGRWTISSSDESEEEKPKPDKPSTSSLLCARQGAANEPRYTCSEAQKAAHKRKISPVKFSNTDS
  VLPPKRQKSGSQEDLGWCLSSSDDELQPEMPQKQAEKVVIKKEKDISAPNDGTAQRTENHGAPACHRLKE
  EEDEYETSGEGQDIWDMLDKGNPFQFYLTRVSGVKPKYNSGALHIKDILSPLFGTLVSSAQFNYCFDVDW
  LVKQYPPEFRKKPILLVHGDKREAKAHLHAQAKPYENISLCQAKLDIAFGTHHTKMMLLLYEEGLRVVIH
  TSNLIHADWHQKTQGIWLSPLYPRIADGTHKSGESPTHFKADLISYLMAYNAPSLKEWIDVIHKHDLSET
  NVYLIGSTPGRFQGSQKDNWGHFRLKKLLKDHASSMPNAESWPVVGQFSSVGSLGADESKWLCSEFKESM
  LTLGKESKTPGKSSVPLYLIYPSVENVRTSLEGYPAGGSLPYSIQTAEKQNWLHSYFHKWSAETSGRSNA
  MPHIKTYMRPSPDFSKIAWFLVTSANLSKAAWGALEKNGTQLMIRSYELGVLFLPSAFGLDSFKVKQKFF
  AGSQEPMATFPVPYDLPPELYGSKDRPWIWNIPYVKAPDTHGNMWVPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018319
• ORF Size: 1824 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018319.4
• RefSeq Size: 3763 bp
• RefSeq ORF: 1827 bp
• Locus ID: 55775
• UniProt ID: Q9NUW8
• Cytogenetics: 14q32.11
• Protein Families: Druggable Genome
• MW: 68.2 kDa
• Gene Summary: The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1). [provided by RefSeq, Aug 2016]