WDR65 (CFAP57) (NM_152498) Human Tagged ORF Clone

CAT#: RC214887

WDR65 (Myc-DDK-tagged)-Human WD repeat domain 65 (WDR65), transcript variant 1

Product Images

Circular map for RC214887

• 

  Western blot validation of overexpression lysate (Cat# LY407504) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214887 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WDR65
• Synonyms: VWS2; WDR65
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214887 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGCCGTGGTAGCTCAGACGCTGCATGTTTTTGGTCTTCGATCCCACGTGGCCAACAATATCTTCT
  ACTTCGATGAACAGATCATTATATTTCCTTCAGGAAATCACTGTGTGAAGTACAATGTGGATCAGAAATG
  GCAAAAATTCATTCCAGGCTCAGAGAAGAGTCAGGGCATGTTGGCCTTGTCCATCAGTCCCAATCGGCGG
  TACCTCGCTATCTCTGAGACTGTGCAAGAAAAACCTGCCATCACCATTTATGAATTGTCATCCATCCCTT
  GCCGGAAGCGCAAAGTTCTTAATAATTTTGACTTCCAAGTTCAGAAATTTATTAGCATGGCTTTTTCTCC
  AGACTCCAAATACCTATTGGCTCAGACGTCACCTCCAGAGTCAAATCTTGTCTACTGGCTGTGGGAAAAA
  CAGAAAGTAATGGCCATTGTTAGAATCGACACTCAGAACAACCCTGTCTACCAGGTGAGCTTCAGTCCAC
  AGGATAACACTCAGGTGTGTGTCACTGGAAATGGGATGTTTAAGCTTCTCCGTTTTGCTGAGGGAACCCT
  GAAGCAAACCAGCTTTCAGAGGGGAGAACCCCAAAACTATCTAGCTCACACCTGGGTGGCTGATGACAAG
  ATTGTCGTTGGCACTGACACAGGCAAACTCTTCCTCTTTGAATCTGGAGATCAGCGTTGGGAGACCAGCA
  TAATGGTCAAGGAACCTACCAATGGCTCAAAGAGCCTGGATGTCATTCAGGAATCAGAGAGCCTGATTGA
  ATTTCCACCAGTCAGTTCTCCACTCCCTTCCTATGAACAGATGGTGGCGGCCAGTAGCCATAGCCAGATG
  TCCATGCCCCAGGTGTTTGCCATTGCAGCCTATTCAAAGGGATTTGCCTGTTCTGCTGGGCCAGGGAGAG
  TTCTGCTGTTTGAGAAGATGGAAGAAAAGGATTTTTACCGTGAGAGCAGAGAAATCAGGATTCCTGTGGA
  CCCGCAGAGCAATGATCCAAGTCAGTCTGACAAACAGGACGTTCTCTGCCTGTGCTTCAGCCCCTCAGAG
  GAAACTCTGGTTGCCAGCACCAGTAAGAACCAACTCTACAGCATCACCATGTCCCTGACAGAGATCAGCA
  AGGGGGAGCCTGCTCACTTTGAGTATTTGATGTATCCATTGCACTCAGCACCCATCACCGGTCTAGCTAC
  CTGCATCCGCAAACCCCTTATAGCCACCTGTTCTCTGGATCGATCCATCCGCCTTTGGAATTATGAAACA
  AACACCCTGGAACTATTTAAGGAATACCAAGAAGAGGCATATTCCATCAGCCTTCATCCATCTGGACACT
  TCATTGTAGTAGGGTTTGCTGACAAACTACGCCTCATGAATCTACTCATTGATGATATACGTTCTTTCAA
  AGAATACTCTGTTAGAGGATGCGGAGAGTGTTCCTTTAGCAATGGAGGTCACCTGTTTGCTGCAGTCAAT
  GGAAATGTGATTCACGTTTACACCACCACGAGCCTAGAGAACATCTCAAGCCTGAAAGGACACACAGGGA
  AGATTCGCTCAATTGTGTGGAATGCAGATGATAGCAAACTGATTTCTGGTGGCACAGATGGTGCTGTGTA
  TGAATGGAATCTGTCCACAGGAAAGAGAGAGACAGAATGCGTGCTCAAGTCTTGCAGCTACAACTGTGTT
  ACTGTCTCCCCCGATGCCAAAATTATCTTTGCTGTTGGATCAGACCACACCCTCAAGGAGATTGCAGATT
  CCTTGATCCTTCGAGAGATATCGGCGTTTGATGTCACCTACACCGCCATTGTCATCTCACATTCTGGACG
  CATGATGTTTGTGGGCACCTCGGTGGGAACCATTCGTGCCATGAAGTACCCTCTGCCTCTGCAGAAGGAA
  TTCAATGAGTACCAGGCCCATGCCGGTCCTATCACCAAGGTGAGCAGGGCCCTCTCCCCAGGAACCCAGT
  CCCACACCTGCCTGCTACGTGCCTTGTTCATCCCTTCAACCTCCCAATGTCTTTTCTCTCTCCTTCTTCT
  CTCTTATTTATTCATCCATCATTCATTGAATCACCATCTATTGACTATGAATATACTCTTTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214887 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSAVVAQTLHVFGLRSHVANNIFYFDEQIIIFPSGNHCVKYNVDQKWQKFIPGSEKSQGMLALSISPNRR
  YLAISETVQEKPAITIYELSSIPCRKRKVLNNFDFQVQKFISMAFSPDSKYLLAQTSPPESNLVYWLWEK
  QKVMAIVRIDTQNNPVYQVSFSPQDNTQVCVTGNGMFKLLRFAEGTLKQTSFQRGEPQNYLAHTWVADDK
  IVVGTDTGKLFLFESGDQRWETSIMVKEPTNGSKSLDVIQESESLIEFPPVSSPLPSYEQMVAASSHSQM
  SMPQVFAIAAYSKGFACSAGPGRVLLFEKMEEKDFYRESREIRIPVDPQSNDPSQSDKQDVLCLCFSPSE
  ETLVASTSKNQLYSITMSLTEISKGEPAHFEYLMYPLHSAPITGLATCIRKPLIATCSLDRSIRLWNYET
  NTLELFKEYQEEAYSISLHPSGHFIVVGFADKLRLMNLLIDDIRSFKEYSVRGCGECSFSNGGHLFAAVN
  GNVIHVYTTTSLENISSLKGHTGKIRSIVWNADDSKLISGGTDGAVYEWNLSTGKRETECVLKSCSYNCV
  TVSPDAKIIFAVGSDHTLKEIADSLILREISAFDVTYTAIVISHSGRMMFVGTSVGTIRAMKYPLPLQKE
  FNEYQAHAGPITKVSRALSPGTQSHTCLLRALFIPSTSQCLFSLLLLSYLFIHHSLNHHLLTMNILFV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152498
• ORF Size: 2094 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152498.3, NP_689711.2
• RefSeq Size: 3037 bp
• RefSeq ORF: 2097 bp
• Locus ID: 149465
• UniProt ID: Q96MR6
• Cytogenetics: 1p34.2
• MW: 78.2 kDa
• Gene Summary: This protein encoded by this gene belongs to the WD repeat-containing family of proteins, which function in the formation of protein-protein complexes in a variety of biological pathways. This family member is thought to function in craniofacial development, possibly in the fusion of lip and palate. A missense mutation in this gene is associated with Van der Woude syndrome 2. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2011]