Anoctamin 7 (ANO7) (NM_001001891) Human Tagged ORF Clone

CAT#: RC214788

ANO7 (Myc-DDK-tagged)-Human anoctamin 7 (ANO7), transcript variant NGEP-L

Product Images

Circular map for RC214788

• 

  Western blot validation of overexpression lysate (Cat# LY424262) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214788 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Anoctamin 7
• Synonyms: D-TMPP; DTMPP; IPCA-5; IPCA5; NGEP; PCANAP5; PCANAP5L; TMEM16G
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214788 representing NM_001001891
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGAATGGCTGCCACTGCCTGGGCGGGGCTCCAAGGGCCACCCCTCCCCACCCTCTGTCCCGCAGTGA
  GGACGGGACTCTACTGCCGAGACCAGGCTCACGCTGAGAGGTGGGCCATGACCTCCGAGACCTCTTCCGG
  AAGCCACTGTGCCAGGAGCAGGATGCTGCGGCGACGGGCCCAGGAAGAGGACAGCACCGTCCTGATCGAT
  GTGAGCCCCCCTGAGGCAGAGAAGAGGGGCTCTTACGGGAGCACAGCCCACGCCTCGGAGCCAGGTGGAC
  AGCAAGCGGCCGCCTGCAGAGCTGGGAGTCCTGCCAAGCCCCGGATCGACTTCGTCCTCGTTTGGGAGGA
  GGACCTGAAGCTAGACAGGCAGCAGGACAGTGCCGCCCGGGACAGAACAGACATGCACAGGACCTGGCGG
  GAGACTTTTCTGGATAATCTTCGTGCGGCTGGGCTGTGTGTAGACCAGCAGGACGTCCAGGACGGGAACA
  CCACAGTGCACTACGCCCTCCTCAGCGCCTCCTGGGCTGTGCTCTGCTACTACGCCGAAGACCTGCGCCT
  GAAGCTGCCCTTGCAGGAGTTACCCAACCAGGCCTCCAACTGGTCGGCCGGCCTGCTGGCATGGCTGGGC
  ATCCCCAACGTCCTGCTGGAGGTTGTGCCAGACGTACCCCCCGAGTACTACTCCTGCCGGTTCAGAGTGA
  ACAAGCTGCCACGCTTCCTCGGGAGTGACAACCAGGACACCTTCTTCACAAGCACCAAGAGGCACCAAAT
  TCTGTTTGAGATCCTGGCCAAGACCCCGTATGGCCACGAGAAGAAAAACCTGCTTGGGATCCACCAGCTG
  CTGGCAGAGGGTGTCCTCAGTGCCGCCTTCCCCCTGCATGACGGCCCCTTCAAGACGCCCCCAGAGGGCC
  CGCAGGCTCCACGCCTCAACCAGCGCCAAGTCCTTTTCCAGCACTGGGCGCGCTGGGGCAAGTGGAACAA
  GTACCAGCCCCTGGACCACGTGCGCAGGTACTTCGGGGAGAAGGTGGCCCTCTACTTCGCCTGGCTCGGG
  TTTTACACAGGCTGGCTCCTGCCAGCGGCAGTGGTGGGCACACTGGTGTTCCTGGTGGGCTGCTTCCTGG
  TGTTCTCAGACATACCCACGCAGGAACTGTGTGGCAGCAAGGACAGCTTCGAGATGTGCCCACTTTGCCT
  CGACTGCCCTTTCTGGCTGCTCTCCAGCGCCTGTGCCCTGGCCCAGGCCGGCCGGCTGTTCGACCACGGC
  GGCACCGTGTTCTTCAGCTTGTTCATGGCACTGTGGGCCGTGCTGCTGCTGGAGTACTGGAAGCGGAAGA
  GCGCCACGCTGGCCTACCGCTGGGACTGCTCTGACTACGAGGACACTGAGGAGAGGCCTCGGCCCCAGTT
  TGCCGCCTCAGCCCCCATGACAGCCCCGAACCCCATCACGGGTGAGGACGAGCCCTACTTCCCTGAGAGG
  AGCCGCGCGCGCCGCATGCTGGCCGGCTCTGTGGTGATCGTGGTGATGGTGGCCGTGGTGGTCATGTGCC
  TCGTGTCTATCATCCTGTACCGTGCCATCATGGCCATCGTGGTGTCCAGGTCGGGCAACACCCTTCTCGC
  AGCCTGGGCCTCTCGCATCGCCAGCCTCACGGGGTCTGTAGTGAACCTCGTCTTCATCCTCATCCTCTCC
  AAGATCTATGTATCCCTGGCCCACGTCCTGACACGATGGGAAATGCACCGCACCCAGACCAAGTTCGAGG
  ACGCCTTCACCCTCAAGGTGTTCATCTTCCAGTTCGTCAACTTCTACTCCTCACCCGTCTACATTGCCTT
  CTTCAAGGGCAGGTTTGTGGGATACCCAGGCAACTACCACACCTTGTTTGGAGTCCGCAATGAGGAGTGC
  GCGGCTGGAGGCTGCCTGATCGAGCTGGCACAGGAGCTCCTGGTCATCATGGTGGGCAAGCAGGTCATCA
  ACAACATGCAGGAGGTCCTCATCCCGAAGCTAAAGGGCTGGTGGCAGAAGTTCCGGCTTCGCTCCAAGAA
  GAGGAAGGCGGGAGCTTCTGCAGGGGCTAGCCAGGGGCCCTGGGAGGACGACTATGAGCTTGTGCCCTGT
  GAGGGTCTGTTTGACGAGTACCTGGAAATGGTGCTGCAGTTCGGCTTCGTCACCATCTTCGTGGCCGCCT
  GTCCGCTCGCGCCGCTCTTCGCCCTGCTCAACAACTGGGTGGAGATCCGCTTGGACGCGCGCAAGTTCGT
  CTGCGAGTACCGGCGCCCGGTGGCCGAGCGCGCCCAGGACATCGGCATCTGGTTCCACATCCTGGCGGGC
  CTCACGCACCTGGCGGTCATCAGCAACGCCTTCCTCCTGGCCTTCTCGTCCGACTTCCTGCCGCGCGCCT
  ACTACCGGTGGACCCGCGCCCACGACCTGCGCGGCTTCCTCAACTTCACGCTGGCGCGAGCCCCGTCCTC
  CTTCGCCGCCGCGCACAACCGCACGTGCAGGTATCGGGCTTTCCGGGATGACGATGGACATTATTCCCAG
  ACCTACTGGAATCTTCTTGCCATCCGCCTGGCCTTCGTCATTGTGTTTGAGCATGTGGTTTTCTCCGTTG
  GCCGCCTCCTGGACCTCCTGGTGCCTGACATCCCAGAGTCTGTGGAGATCAAAGTGAAGCGGGAGTACTA
  CCTGGCTAAGCAGGCACTGGCTGAGAATGAGGTTCTTTTTGGAACGAACGGAACAAAGGATGAGCAGCCC
  GAGGGCTCAGAGCTCAGCTCCCACTGGACACCCTTCACGGTTCCCAAGGCCAGCCAGCTGCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214788 representing NM_001001891
  Red=Cloning site Green=Tags(s)
  
  MRMAATAWAGLQGPPLPTLCPAVRTGLYCRDQAHAERWAMTSETSSGSHCARSRMLRRRAQEEDSTVLID
  VSPPEAEKRGSYGSTAHASEPGGQQAAACRAGSPAKPRIDFVLVWEEDLKLDRQQDSAARDRTDMHRTWR
  ETFLDNLRAAGLCVDQQDVQDGNTTVHYALLSASWAVLCYYAEDLRLKLPLQELPNQASNWSAGLLAWLG
  IPNVLLEVVPDVPPEYYSCRFRVNKLPRFLGSDNQDTFFTSTKRHQILFEILAKTPYGHEKKNLLGIHQL
  LAEGVLSAAFPLHDGPFKTPPEGPQAPRLNQRQVLFQHWARWGKWNKYQPLDHVRRYFGEKVALYFAWLG
  FYTGWLLPAAVVGTLVFLVGCFLVFSDIPTQELCGSKDSFEMCPLCLDCPFWLLSSACALAQAGRLFDHG
  GTVFFSLFMALWAVLLLEYWKRKSATLAYRWDCSDYEDTEERPRPQFAASAPMTAPNPITGEDEPYFPER
  SRARRMLAGSVVIVVMVAVVVMCLVSIILYRAIMAIVVSRSGNTLLAAWASRIASLTGSVVNLVFILILS
  KIYVSLAHVLTRWEMHRTQTKFEDAFTLKVFIFQFVNFYSSPVYIAFFKGRFVGYPGNYHTLFGVRNEEC
  AAGGCLIELAQELLVIMVGKQVINNMQEVLIPKLKGWWQKFRLRSKKRKAGASAGASQGPWEDDYELVPC
  EGLFDEYLEMVLQFGFVTIFVAACPLAPLFALLNNWVEIRLDARKFVCEYRRPVAERAQDIGIWFHILAG
  LTHLAVISNAFLLAFSSDFLPRAYYRWTRAHDLRGFLNFTLARAPSSFAAAHNRTCRYRAFRDDDGHYSQ
  TYWNLLAIRLAFVIVFEHVVFSVGRLLDLLVPDIPESVEIKVKREYYLAKQALAENEVLFGTNGTKDEQP
  EGSELSSHWTPFTVPKASQLQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001891
• ORF Size: 2796 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001891.1, NP_001001891.1
• RefSeq Size: 3308 bp
• RefSeq ORF: 2802 bp
• Locus ID: 50636
• UniProt ID: Q6IWH7
• Cytogenetics: 2q37.3
• Protein Families: Transmembrane
• MW: 105.4 kDa
• Gene Summary: This prostate-specific gene encodes a cytoplasmic protein, as well as a polytopic membrane protein which may serve as a target in prostate cancer diagnosis and immunotherapy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2011]